Z-scan method to measure the nonlinear optical behavior of cells for evaluating the cytotoxic effects of chemotherapy and hyperthermia treatments
The effects of new treatments must be investigated in vitro before using clinically or in vivo. The aim of this study was to introduce the Z-scan technique as a fast, accurate, inexpensive, and safe in vitro method to distinguish the cytotoxic effects of various treatments. C6 and OLN-93 cell lines...
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| Veröffentlicht in: | Lasers in medical science 2021-07, Vol.36 (5), p.1067-1075 |
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| creator | Minaei, Soraya Emamgholizadeh Ghader, Alireza Ardakani, Ali Abbasian Khoei, Samideh Asgari, Hamid Ara, Mohammad Hosein Majles |
| description | The effects of new treatments must be investigated in vitro before using clinically or in vivo. The aim of this study was to introduce the Z-scan technique as a fast, accurate, inexpensive, and safe in vitro method to distinguish the cytotoxic effects of various treatments. C6 and OLN-93 cell lines were prepared and treated with Temozolomide (TMZ), radiofrequency hyperthermia (HT), and chemo-hyperthermia (HT+TMZ). The cytotoxic effects of different treatments on both cell lines were evaluated using colony formation assay and Z-scan method. The results of colony assay showed that the surviving fraction (SF) of C6 cells treated with TMZ, HT, and HT + TMZ were significantly decreased compared to the control group. Whereas, hyperthermia treatment had no significant effect on the SF of OLN-93 cells. The results of Z-scan technique indicated that the control group of C6 cells had the negative nonlinear refractive index (
n
2
). Whereas, the C6 cells treated with HT, TMZ, and HT + TMZ had the positive
n
2
index. The sign of
n
2
index in the control and HT groups of OLN-93 cells was positive but treatment of cells with TMZ and HT + TMZ changed the sign of it. Moreover, with increasing the cytotoxic effects of different treatments, the SF value of both cell lines decreased and the magnitude of
n
2
index increased. The results of Z-scan technique were completely in line with the results of colony assay. Therefore, Z-scan method could distinguish the cytotoxic effects of various treatments by examining the nonlinear optical properties of the samples. |
| doi_str_mv | 10.1007/s10103-020-03148-2 |
| format | Article |
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n
2
). Whereas, the C6 cells treated with HT, TMZ, and HT + TMZ had the positive
n
2
index. The sign of
n
2
index in the control and HT groups of OLN-93 cells was positive but treatment of cells with TMZ and HT + TMZ changed the sign of it. Moreover, with increasing the cytotoxic effects of different treatments, the SF value of both cell lines decreased and the magnitude of
n
2
index increased. The results of Z-scan technique were completely in line with the results of colony assay. Therefore, Z-scan method could distinguish the cytotoxic effects of various treatments by examining the nonlinear optical properties of the samples.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-020-03148-2</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Assaying ; Cell lines ; Chemotherapy ; Colonies ; Cytotoxicity ; Dentistry ; Evaluation ; Fever ; Hyperthermia ; In vitro methods and tests ; In vivo methods and tests ; Lasers ; Medicine ; Medicine & Public Health ; Nonlinear optics ; Optical Devices ; Optical properties ; Optics ; Original Article ; Photonics ; Quantum Optics ; Radio frequency ; Refractivity ; Temozolomide</subject><ispartof>Lasers in medical science, 2021-07, Vol.36 (5), p.1067-1075</ispartof><rights>Springer-Verlag London Ltd., part of Springer Nature 2020</rights><rights>Springer-Verlag London Ltd., part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-2d0acdbe050ff7a23c8bb804e72358e101ad1a5c28dab91f2e2854430ff08c373</citedby><cites>FETCH-LOGICAL-c352t-2d0acdbe050ff7a23c8bb804e72358e101ad1a5c28dab91f2e2854430ff08c373</cites><orcidid>0000-0002-7191-5094</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-020-03148-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-020-03148-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Minaei, Soraya Emamgholizadeh</creatorcontrib><creatorcontrib>Ghader, Alireza</creatorcontrib><creatorcontrib>Ardakani, Ali Abbasian</creatorcontrib><creatorcontrib>Khoei, Samideh</creatorcontrib><creatorcontrib>Asgari, Hamid</creatorcontrib><creatorcontrib>Ara, Mohammad Hosein Majles</creatorcontrib><title>Z-scan method to measure the nonlinear optical behavior of cells for evaluating the cytotoxic effects of chemotherapy and hyperthermia treatments</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><description>The effects of new treatments must be investigated in vitro before using clinically or in vivo. The aim of this study was to introduce the Z-scan technique as a fast, accurate, inexpensive, and safe in vitro method to distinguish the cytotoxic effects of various treatments. C6 and OLN-93 cell lines were prepared and treated with Temozolomide (TMZ), radiofrequency hyperthermia (HT), and chemo-hyperthermia (HT+TMZ). The cytotoxic effects of different treatments on both cell lines were evaluated using colony formation assay and Z-scan method. The results of colony assay showed that the surviving fraction (SF) of C6 cells treated with TMZ, HT, and HT + TMZ were significantly decreased compared to the control group. Whereas, hyperthermia treatment had no significant effect on the SF of OLN-93 cells. The results of Z-scan technique indicated that the control group of C6 cells had the negative nonlinear refractive index (
n
2
). Whereas, the C6 cells treated with HT, TMZ, and HT + TMZ had the positive
n
2
index. The sign of
n
2
index in the control and HT groups of OLN-93 cells was positive but treatment of cells with TMZ and HT + TMZ changed the sign of it. Moreover, with increasing the cytotoxic effects of different treatments, the SF value of both cell lines decreased and the magnitude of
n
2
index increased. The results of Z-scan technique were completely in line with the results of colony assay. Therefore, Z-scan method could distinguish the cytotoxic effects of various treatments by examining the nonlinear optical properties of the samples.</description><subject>Assaying</subject><subject>Cell lines</subject><subject>Chemotherapy</subject><subject>Colonies</subject><subject>Cytotoxicity</subject><subject>Dentistry</subject><subject>Evaluation</subject><subject>Fever</subject><subject>Hyperthermia</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Lasers</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nonlinear optics</subject><subject>Optical Devices</subject><subject>Optical properties</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Quantum Optics</subject><subject>Radio frequency</subject><subject>Refractivity</subject><subject>Temozolomide</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kMFq3DAQhkVpoNu0L9CToGc1I8leK8cSkiYQ6KWF0osYy6PYwZZcSRu6j5E3rnY3kFtPGs18_wx8jH2S8EUCdBdZggQtQIEALRsj1Bu2kY1uxRaaX2_ZBtTWCHOp5Dv2PudHANltpd6w598iOwx8oTLGgZdYK8y7RLyMxEMM8xQIE49rmRzOvKcRn6ZYG547mufMff3QE847LFN4OMbcvsQS_06Ok_fkSj7SIy2xThOue45h4ON-pXRoLBPykgjLQqHkD-zM45zp48t7zn7eXP-4uhX337_dXX29F063qgg1ALqhJ2jB-w6VdqbvDTTUKd0aqj5wkNg6ZQbsL6VXpEzbNLrSYJzu9Dn7fNq7pvhnR7nYx7hLoZ606gAqMI2plDpRLsWcE3m7pmnBtLcS7EG9Pam3Vb09qreqhvQplCscHii9rv5P6h_TN4pH</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Minaei, Soraya Emamgholizadeh</creator><creator>Ghader, Alireza</creator><creator>Ardakani, Ali Abbasian</creator><creator>Khoei, Samideh</creator><creator>Asgari, Hamid</creator><creator>Ara, Mohammad Hosein Majles</creator><general>Springer London</general><general>Springer Nature 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method to measure the nonlinear optical behavior of cells for evaluating the cytotoxic effects of chemotherapy and hyperthermia treatments</title><author>Minaei, Soraya Emamgholizadeh ; Ghader, Alireza ; Ardakani, Ali Abbasian ; Khoei, Samideh ; Asgari, Hamid ; Ara, Mohammad Hosein Majles</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-2d0acdbe050ff7a23c8bb804e72358e101ad1a5c28dab91f2e2854430ff08c373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Assaying</topic><topic>Cell lines</topic><topic>Chemotherapy</topic><topic>Colonies</topic><topic>Cytotoxicity</topic><topic>Dentistry</topic><topic>Evaluation</topic><topic>Fever</topic><topic>Hyperthermia</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Lasers</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nonlinear optics</topic><topic>Optical Devices</topic><topic>Optical properties</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Quantum Optics</topic><topic>Radio frequency</topic><topic>Refractivity</topic><topic>Temozolomide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minaei, Soraya Emamgholizadeh</creatorcontrib><creatorcontrib>Ghader, Alireza</creatorcontrib><creatorcontrib>Ardakani, Ali Abbasian</creatorcontrib><creatorcontrib>Khoei, Samideh</creatorcontrib><creatorcontrib>Asgari, Hamid</creatorcontrib><creatorcontrib>Ara, Mohammad Hosein Majles</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical 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science</jtitle><stitle>Lasers Med Sci</stitle><date>2021-07-01</date><risdate>2021</risdate><volume>36</volume><issue>5</issue><spage>1067</spage><epage>1075</epage><pages>1067-1075</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>The effects of new treatments must be investigated in vitro before using clinically or in vivo. The aim of this study was to introduce the Z-scan technique as a fast, accurate, inexpensive, and safe in vitro method to distinguish the cytotoxic effects of various treatments. C6 and OLN-93 cell lines were prepared and treated with Temozolomide (TMZ), radiofrequency hyperthermia (HT), and chemo-hyperthermia (HT+TMZ). The cytotoxic effects of different treatments on both cell lines were evaluated using colony formation assay and Z-scan method. The results of colony assay showed that the surviving fraction (SF) of C6 cells treated with TMZ, HT, and HT + TMZ were significantly decreased compared to the control group. Whereas, hyperthermia treatment had no significant effect on the SF of OLN-93 cells. The results of Z-scan technique indicated that the control group of C6 cells had the negative nonlinear refractive index (
n
2
). Whereas, the C6 cells treated with HT, TMZ, and HT + TMZ had the positive
n
2
index. The sign of
n
2
index in the control and HT groups of OLN-93 cells was positive but treatment of cells with TMZ and HT + TMZ changed the sign of it. Moreover, with increasing the cytotoxic effects of different treatments, the SF value of both cell lines decreased and the magnitude of
n
2
index increased. The results of Z-scan technique were completely in line with the results of colony assay. Therefore, Z-scan method could distinguish the cytotoxic effects of various treatments by examining the nonlinear optical properties of the samples.</abstract><cop>London</cop><pub>Springer London</pub><doi>10.1007/s10103-020-03148-2</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-7191-5094</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Lasers in medical science, 2021-07, Vol.36 (5), p.1067-1075 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Assaying Cell lines Chemotherapy Colonies Cytotoxicity Dentistry Evaluation Fever Hyperthermia In vitro methods and tests In vivo methods and tests Lasers Medicine Medicine & Public Health Nonlinear optics Optical Devices Optical properties Optics Original Article Photonics Quantum Optics Radio frequency Refractivity Temozolomide |
| title | Z-scan method to measure the nonlinear optical behavior of cells for evaluating the cytotoxic effects of chemotherapy and hyperthermia treatments |
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