Study of the relationship between untypable and typable isolates of Streptococcus suis recovered from clinically ill and healthy nursery pigs
•Study isolates grouped into 17 distinct groups based on a cps gene tree.•Untypable isolates were detected in 12 phylogenetic tree groups.•Most farms had untypable and typable isolates in different cps groups.•All untypable isolates in systemic sites were detected in sampling sites with typable isol...
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| Veröffentlicht in: | Veterinary microbiology 2021-06, Vol.257, p.109064-109064, Article 109064 |
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| container_title | Veterinary microbiology |
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| creator | Denich, Leann Farzan, Abdolvahab Friendship, Robert Arndt, Emily Ricker, Nicole Gottschalk, Marcelo Poljak, Zvonimir |
| description | •Study isolates grouped into 17 distinct groups based on a cps gene tree.•Untypable isolates were detected in 12 phylogenetic tree groups.•Most farms had untypable and typable isolates in different cps groups.•All untypable isolates in systemic sites were detected in sampling sites with typable isolates.•All untypable isolates in systemic sites had protein sequences |
| doi_str_mv | 10.1016/j.vetmic.2021.109064 |
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Streptococcus suis naturally colonizes the upper respiratory tract of pigs and can lead to severe disease conditions. Although there are several serotypes associated with disease, untypable isolates have also been observed. The objective of this study was to investigate the relatedness of untypable S. suis isolates detected in clinical cases and healthy pigs in Ontario, Canada, and their relation to typing serotypes. One hundred fifty-six isolates obtained from 33 cases and 26 farm-and-pen-matched control pigs were sequenced using Illumina HiSeq sequencing. Protein sequences of the capsular polysaccharide genes (cps) were identified and analyzed using a maximum likelihood tree. Among the 27 untypable isolates, 3 were from systemic sites of cases and 13 and 11 were from upper respiratory sites of cases and controls, respectively. One hundred fifty-six isolates were grouped into 17 distinct groups based on the cps gene tree. Isolates from these 17 distinct individual cps groups were distributed among a minimum of one farm and maximum of eight farms. Untypable isolates were detected in 12 of those groups and each cps group had untypable isolates present amongst multiple farms. Interestingly, the three systemic untypable isolates not only coexisted with other serotypes found in the same location of the same pigs but were also found among different cps groups. These isolates are of interest and warrant further investigation. Overall, a wide diversity of S. suis among untypable isolates was observed in this study.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2021.109064</identifier><identifier>PMID: 33892450</identifier><language>eng</language><publisher>AMSTERDAM: Elsevier B.V</publisher><subject>Capsular polysaccharides ; CPS gene ; Farms ; Life Sciences & Biomedicine ; Microbiology ; Respiratory tract ; Science & Technology ; Serotypes ; Streptococcus infections ; Streptococcus suis ; Swine ; Untypable ; Veterinary Sciences</subject><ispartof>Veterinary microbiology, 2021-06, Vol.257, p.109064-109064, Article 109064</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Jun 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>2</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000651345500003</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c390t-d4268974c376783b4cfd65f71460e60c5db0ab130ea8738d982d7dc582ba6b823</citedby><cites>FETCH-LOGICAL-c390t-d4268974c376783b4cfd65f71460e60c5db0ab130ea8738d982d7dc582ba6b823</cites><orcidid>0000-0001-5706-5399 ; 0000-0003-1979-6937</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2021.109064$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,39263,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33892450$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Denich, Leann</creatorcontrib><creatorcontrib>Farzan, Abdolvahab</creatorcontrib><creatorcontrib>Friendship, Robert</creatorcontrib><creatorcontrib>Arndt, Emily</creatorcontrib><creatorcontrib>Ricker, Nicole</creatorcontrib><creatorcontrib>Gottschalk, Marcelo</creatorcontrib><creatorcontrib>Poljak, Zvonimir</creatorcontrib><title>Study of the relationship between untypable and typable isolates of Streptococcus suis recovered from clinically ill and healthy nursery pigs</title><title>Veterinary microbiology</title><addtitle>VET MICROBIOL</addtitle><addtitle>Vet Microbiol</addtitle><description>•Study isolates grouped into 17 distinct groups based on a cps gene tree.•Untypable isolates were detected in 12 phylogenetic tree groups.•Most farms had untypable and typable isolates in different cps groups.•All untypable isolates in systemic sites were detected in sampling sites with typable isolates.•All untypable isolates in systemic sites had protein sequences <50 % similar to any other typable isolate in the study.
Streptococcus suis naturally colonizes the upper respiratory tract of pigs and can lead to severe disease conditions. Although there are several serotypes associated with disease, untypable isolates have also been observed. The objective of this study was to investigate the relatedness of untypable S. suis isolates detected in clinical cases and healthy pigs in Ontario, Canada, and their relation to typing serotypes. One hundred fifty-six isolates obtained from 33 cases and 26 farm-and-pen-matched control pigs were sequenced using Illumina HiSeq sequencing. Protein sequences of the capsular polysaccharide genes (cps) were identified and analyzed using a maximum likelihood tree. Among the 27 untypable isolates, 3 were from systemic sites of cases and 13 and 11 were from upper respiratory sites of cases and controls, respectively. One hundred fifty-six isolates were grouped into 17 distinct groups based on the cps gene tree. Isolates from these 17 distinct individual cps groups were distributed among a minimum of one farm and maximum of eight farms. Untypable isolates were detected in 12 of those groups and each cps group had untypable isolates present amongst multiple farms. Interestingly, the three systemic untypable isolates not only coexisted with other serotypes found in the same location of the same pigs but were also found among different cps groups. These isolates are of interest and warrant further investigation. Overall, a wide diversity of S. suis among untypable isolates was observed in this study.</description><subject>Capsular polysaccharides</subject><subject>CPS gene</subject><subject>Farms</subject><subject>Life Sciences & Biomedicine</subject><subject>Microbiology</subject><subject>Respiratory tract</subject><subject>Science & Technology</subject><subject>Serotypes</subject><subject>Streptococcus infections</subject><subject>Streptococcus suis</subject><subject>Swine</subject><subject>Untypable</subject><subject>Veterinary Sciences</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><recordid>eNqNkc2KFDEURgtRnHb0DUQCboSh2_xVKrURpHFUGHAxug6p5JadJl0pk1QP9RC-s-mpnlm4EFe5hPN9JPdU1WuCNwQT8X6_OUI-OLOhmJJy1WLBn1QrIhu2pjWnT6sVZo1cE8Lqi-pFSnuMMW8Ffl5dMCZbymu8qn7f5snOKPQo7wBF8Dq7MKSdG1EH-Q5gQNOQ51F3HpAeLHqYXQqFhXSK3uYIYw4mGDMllCaXSpMJR4hgUR_DARnvBme09zNy3t8X7UD7vJvRMMUEcUaj-5leVs967RO8Op-X1Y_rT9-3X9Y33z5_3X68WRvW4ry2nArZNtywRjSSddz0VtR9Q7jAILCpbYd1RxgGXbYhbSupbaypJe206CRll9W7pXeM4dcEKauDSwa81wOEKSlaE0lpQ2hb0Ld_ofswxaG8rlCcF0hQVii-UCaGlCL0aozuoOOsCFYnXWqvFl3qpEstukrszbl86g5gH0MPfgogF-AOutAn42Aw8IgVoaImjNd1mTDbunxvbxuKshK9-v9ooT8sNJS1Hx1EdU5YV1RmZYP791f-ALDtyt4</recordid><startdate>202106</startdate><enddate>202106</enddate><creator>Denich, Leann</creator><creator>Farzan, Abdolvahab</creator><creator>Friendship, Robert</creator><creator>Arndt, Emily</creator><creator>Ricker, Nicole</creator><creator>Gottschalk, Marcelo</creator><creator>Poljak, Zvonimir</creator><general>Elsevier B.V</general><general>Elsevier</general><general>Elsevier BV</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5706-5399</orcidid><orcidid>https://orcid.org/0000-0003-1979-6937</orcidid></search><sort><creationdate>202106</creationdate><title>Study of the relationship between untypable and typable isolates of Streptococcus suis recovered from clinically ill and healthy nursery pigs</title><author>Denich, Leann ; Farzan, Abdolvahab ; Friendship, Robert ; Arndt, Emily ; Ricker, Nicole ; Gottschalk, Marcelo ; Poljak, Zvonimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-d4268974c376783b4cfd65f71460e60c5db0ab130ea8738d982d7dc582ba6b823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Capsular polysaccharides</topic><topic>CPS gene</topic><topic>Farms</topic><topic>Life Sciences & Biomedicine</topic><topic>Microbiology</topic><topic>Respiratory tract</topic><topic>Science & Technology</topic><topic>Serotypes</topic><topic>Streptococcus infections</topic><topic>Streptococcus suis</topic><topic>Swine</topic><topic>Untypable</topic><topic>Veterinary Sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Denich, Leann</creatorcontrib><creatorcontrib>Farzan, Abdolvahab</creatorcontrib><creatorcontrib>Friendship, Robert</creatorcontrib><creatorcontrib>Arndt, Emily</creatorcontrib><creatorcontrib>Ricker, Nicole</creatorcontrib><creatorcontrib>Gottschalk, Marcelo</creatorcontrib><creatorcontrib>Poljak, Zvonimir</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Denich, Leann</au><au>Farzan, Abdolvahab</au><au>Friendship, Robert</au><au>Arndt, Emily</au><au>Ricker, Nicole</au><au>Gottschalk, Marcelo</au><au>Poljak, Zvonimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of the relationship between untypable and typable isolates of Streptococcus suis recovered from clinically ill and healthy nursery pigs</atitle><jtitle>Veterinary microbiology</jtitle><stitle>VET MICROBIOL</stitle><addtitle>Vet Microbiol</addtitle><date>2021-06</date><risdate>2021</risdate><volume>257</volume><spage>109064</spage><epage>109064</epage><pages>109064-109064</pages><artnum>109064</artnum><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>•Study isolates grouped into 17 distinct groups based on a cps gene tree.•Untypable isolates were detected in 12 phylogenetic tree groups.•Most farms had untypable and typable isolates in different cps groups.•All untypable isolates in systemic sites were detected in sampling sites with typable isolates.•All untypable isolates in systemic sites had protein sequences <50 % similar to any other typable isolate in the study.
Streptococcus suis naturally colonizes the upper respiratory tract of pigs and can lead to severe disease conditions. Although there are several serotypes associated with disease, untypable isolates have also been observed. The objective of this study was to investigate the relatedness of untypable S. suis isolates detected in clinical cases and healthy pigs in Ontario, Canada, and their relation to typing serotypes. One hundred fifty-six isolates obtained from 33 cases and 26 farm-and-pen-matched control pigs were sequenced using Illumina HiSeq sequencing. Protein sequences of the capsular polysaccharide genes (cps) were identified and analyzed using a maximum likelihood tree. Among the 27 untypable isolates, 3 were from systemic sites of cases and 13 and 11 were from upper respiratory sites of cases and controls, respectively. One hundred fifty-six isolates were grouped into 17 distinct groups based on the cps gene tree. Isolates from these 17 distinct individual cps groups were distributed among a minimum of one farm and maximum of eight farms. Untypable isolates were detected in 12 of those groups and each cps group had untypable isolates present amongst multiple farms. Interestingly, the three systemic untypable isolates not only coexisted with other serotypes found in the same location of the same pigs but were also found among different cps groups. These isolates are of interest and warrant further investigation. Overall, a wide diversity of S. suis among untypable isolates was observed in this study.</abstract><cop>AMSTERDAM</cop><pub>Elsevier B.V</pub><pmid>33892450</pmid><doi>10.1016/j.vetmic.2021.109064</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5706-5399</orcidid><orcidid>https://orcid.org/0000-0003-1979-6937</orcidid></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present); Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /> |
| subjects | Capsular polysaccharides CPS gene Farms Life Sciences & Biomedicine Microbiology Respiratory tract Science & Technology Serotypes Streptococcus infections Streptococcus suis Swine Untypable Veterinary Sciences |
| title | Study of the relationship between untypable and typable isolates of Streptococcus suis recovered from clinically ill and healthy nursery pigs |
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