Heterotrophic Microalgae Cultivation Using Jellyfish Protein Extract to Produce Bioactive Peptides
Jellyfish are often seen as a nuisance, and while attempts have been made to use jellyfish waste as fertilizer and food, they have not proved fruitful. In this study, proteins extracted from jellyfish ( Aurelia aurita ) are investigated as a novel nutrient source in culture medium and tested on aeri...
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| Veröffentlicht in: | Waste and biomass valorization 2021, Vol.12 (7), p.3695-3702 |
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| creator | Aburai, Nobuhiro Oe, Junko Abe, Katsuya |
| description | Jellyfish are often seen as a nuisance, and while attempts have been made to use jellyfish waste as fertilizer and food, they have not proved fruitful. In this study, proteins extracted from jellyfish (
Aurelia aurita
) are investigated as a novel nutrient source in culture medium and tested on aerial microalgae under heterotrophic condition. Results demonstrated that
Vischeria helvetica
KGU-Y001 heterotrophically grew in the extract-containing culture medium in the presence of light. Decomposition of the extracted protein in the culture medium containing
V
.
helvetica
cells was verified both in the presence and absence of light. The inhibitory activity of angiotensin I-converting enzyme (ACE) was measured in the culture medium when in the presence of light. The hydrolysates in the medium were separated using a gel filtration column and a peptide fraction with approximately 400 Da, and high ACE inhibitory activity (IC
50
= 72 µg mL
−1
) was obtained. The results presented here will help to facilitate the development of culture systems with jellyfish waste as a resource for culture medium.
Graphic Abstract |
| doi_str_mv | 10.1007/s12649-020-01261-5 |
| format | Article |
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Aurelia aurita
) are investigated as a novel nutrient source in culture medium and tested on aerial microalgae under heterotrophic condition. Results demonstrated that
Vischeria helvetica
KGU-Y001 heterotrophically grew in the extract-containing culture medium in the presence of light. Decomposition of the extracted protein in the culture medium containing
V
.
helvetica
cells was verified both in the presence and absence of light. The inhibitory activity of angiotensin I-converting enzyme (ACE) was measured in the culture medium when in the presence of light. The hydrolysates in the medium were separated using a gel filtration column and a peptide fraction with approximately 400 Da, and high ACE inhibitory activity (IC
50
= 72 µg mL
−1
) was obtained. The results presented here will help to facilitate the development of culture systems with jellyfish waste as a resource for culture medium.
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Aurelia aurita
) are investigated as a novel nutrient source in culture medium and tested on aerial microalgae under heterotrophic condition. Results demonstrated that
Vischeria helvetica
KGU-Y001 heterotrophically grew in the extract-containing culture medium in the presence of light. Decomposition of the extracted protein in the culture medium containing
V
.
helvetica
cells was verified both in the presence and absence of light. The inhibitory activity of angiotensin I-converting enzyme (ACE) was measured in the culture medium when in the presence of light. The hydrolysates in the medium were separated using a gel filtration column and a peptide fraction with approximately 400 Da, and high ACE inhibitory activity (IC
50
= 72 µg mL
−1
) was obtained. The results presented here will help to facilitate the development of culture systems with jellyfish waste as a resource for culture medium.
Graphic Abstract</description><subject>Algae</subject><subject>Angiotensin</subject><subject>Angiotensin I</subject><subject>Aquatic microorganisms</subject><subject>Cell culture</subject><subject>Cnidaria</subject><subject>Culture media</subject><subject>Engineering</subject><subject>Environment</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Gel filtration</subject><subject>Hydrolysates</subject><subject>Industrial Pollution Prevention</subject><subject>Microalgae</subject><subject>Original Paper</subject><subject>Peptides</subject><subject>Peptidyl-dipeptidase A</subject><subject>Proteins</subject><subject>Renewable and Green Energy</subject><subject>Waste Management/Waste Technology</subject><issn>1877-2641</issn><issn>1877-265X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9UMFOAjEQ3RhNJMgPeGriebXttnT3qARFg5GDJN6adplCybpd2y6Rv7eI0ZuneZl5783My7JLgq8JxuImEDpmVY4pznGCJOcn2YCUQuR0zN9OfzEj59kohC3GmBJS0kIMMj2DCN5F77qNrdGzrb1TzVoBmvRNtDsVrWvRMth2jZ6gafbGhg1aJAXYFk0_o1d1RNEdWqu-BnRnXerYHaAFdNGuIFxkZ0Y1AUY_dZgt76evk1k-f3l4nNzO87rAIuaG6FUpFDaagSkVUG20EryGdK0AIWiFCWNYkALrMQhTMcKJ1qTihT5MimF2dfTtvPvoIUS5db1v00pJOSMlZyWliUWPrPRoCB6M7Lx9V34vCZaHOOUxTpnilN9xSp5ExVEUErldg_-z_kf1BZHYeIc</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Aburai, Nobuhiro</creator><creator>Oe, Junko</creator><creator>Abe, Katsuya</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-4977-9962</orcidid></search><sort><creationdate>2021</creationdate><title>Heterotrophic Microalgae Cultivation Using Jellyfish Protein Extract to Produce Bioactive Peptides</title><author>Aburai, Nobuhiro ; Oe, Junko ; Abe, Katsuya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-f1bd87a0fb4ef8ae2bfba75ce0027e7729014407130b6e7f94151bb1953b01443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Algae</topic><topic>Angiotensin</topic><topic>Angiotensin I</topic><topic>Aquatic microorganisms</topic><topic>Cell culture</topic><topic>Cnidaria</topic><topic>Culture media</topic><topic>Engineering</topic><topic>Environment</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Gel filtration</topic><topic>Hydrolysates</topic><topic>Industrial Pollution Prevention</topic><topic>Microalgae</topic><topic>Original Paper</topic><topic>Peptides</topic><topic>Peptidyl-dipeptidase A</topic><topic>Proteins</topic><topic>Renewable and Green Energy</topic><topic>Waste Management/Waste Technology</topic><toplevel>online_resources</toplevel><creatorcontrib>Aburai, Nobuhiro</creatorcontrib><creatorcontrib>Oe, Junko</creatorcontrib><creatorcontrib>Abe, Katsuya</creatorcontrib><collection>CrossRef</collection><jtitle>Waste and biomass valorization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aburai, Nobuhiro</au><au>Oe, Junko</au><au>Abe, Katsuya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterotrophic Microalgae Cultivation Using Jellyfish Protein Extract to Produce Bioactive Peptides</atitle><jtitle>Waste and biomass valorization</jtitle><stitle>Waste Biomass Valor</stitle><date>2021</date><risdate>2021</risdate><volume>12</volume><issue>7</issue><spage>3695</spage><epage>3702</epage><pages>3695-3702</pages><issn>1877-2641</issn><eissn>1877-265X</eissn><abstract>Jellyfish are often seen as a nuisance, and while attempts have been made to use jellyfish waste as fertilizer and food, they have not proved fruitful. In this study, proteins extracted from jellyfish (
Aurelia aurita
) are investigated as a novel nutrient source in culture medium and tested on aerial microalgae under heterotrophic condition. Results demonstrated that
Vischeria helvetica
KGU-Y001 heterotrophically grew in the extract-containing culture medium in the presence of light. Decomposition of the extracted protein in the culture medium containing
V
.
helvetica
cells was verified both in the presence and absence of light. The inhibitory activity of angiotensin I-converting enzyme (ACE) was measured in the culture medium when in the presence of light. The hydrolysates in the medium were separated using a gel filtration column and a peptide fraction with approximately 400 Da, and high ACE inhibitory activity (IC
50
= 72 µg mL
−1
) was obtained. The results presented here will help to facilitate the development of culture systems with jellyfish waste as a resource for culture medium.
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| ispartof | Waste and biomass valorization, 2021, Vol.12 (7), p.3695-3702 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Algae Angiotensin Angiotensin I Aquatic microorganisms Cell culture Cnidaria Culture media Engineering Environment Environmental Engineering/Biotechnology Gel filtration Hydrolysates Industrial Pollution Prevention Microalgae Original Paper Peptides Peptidyl-dipeptidase A Proteins Renewable and Green Energy Waste Management/Waste Technology |
| title | Heterotrophic Microalgae Cultivation Using Jellyfish Protein Extract to Produce Bioactive Peptides |
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