Evaluation of Mycobacterium tuberculosis ripA gene to detect antibiotic resistance
Mycobacterium tuberculosis infection has remained a public health threat in Indonesia. This infection is complicated by the antibiotic-resistant of M. tuberculosis strains. The common essential in resistance comes from a mutation in genomic DNA. ripA gene is one of the regions critical in the replic...
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| Veröffentlicht in: | Journal of physics. Conference series 2021-06, Vol.1918 (5), p.52014 |
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| creator | Koentjoro, M P Rahayu, D S Donastin, A Prasetyo, E N |
| description | Mycobacterium tuberculosis
infection has remained a public health threat in Indonesia. This infection is complicated by the antibiotic-resistant of
M. tuberculosis
strains. The common essential in resistance comes from a mutation in genomic DNA. ripA gene is one of the regions critical in the replication and persistence of
M. tuberculosis
in their resistance. This gene has responsible for cell wall polymer peptidoglycan. The objective of this research was to evaluate the ripA gene in antibiotic resistance. This is to investigate and compare the ripA gene sequences of
M. tuberculosis
at an unprecedented rate. A total of five specimens of
M. tuberculosis
were isolated from tuberculosis patients with rifampicin resistance. The ripA gene from
M. tuberculosis
was isolated and amplified using a design primer for ripA N-terminal domain of peptidoglycan hydrolase. Further, ripA gene was analyzed using the Sanger method sequencing. The data were analyzed and compared using
M. tuberculosis
H37Rv from the National Center for Biotechnology Information (NCBI). In addition, the sequence was analyzed with multiple sequence alignment (Clustal IW) to identify the mutation. Our result suggests that the evaluation of genes in M. tuberculosis isolates revealed sequence variation in ripA regions (Ala701Gly). Understanding these mutations implies an evaluation of antibiotic-resistant. Furthermore, this information implies local diagnostic and treatment guidelines to cell-wall targeting antibiotics. |
| doi_str_mv | 10.1088/1742-6596/1918/5/052014 |
| format | Article |
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infection has remained a public health threat in Indonesia. This infection is complicated by the antibiotic-resistant of
M. tuberculosis
strains. The common essential in resistance comes from a mutation in genomic DNA. ripA gene is one of the regions critical in the replication and persistence of
M. tuberculosis
in their resistance. This gene has responsible for cell wall polymer peptidoglycan. The objective of this research was to evaluate the ripA gene in antibiotic resistance. This is to investigate and compare the ripA gene sequences of
M. tuberculosis
at an unprecedented rate. A total of five specimens of
M. tuberculosis
were isolated from tuberculosis patients with rifampicin resistance. The ripA gene from
M. tuberculosis
was isolated and amplified using a design primer for ripA N-terminal domain of peptidoglycan hydrolase. Further, ripA gene was analyzed using the Sanger method sequencing. The data were analyzed and compared using
M. tuberculosis
H37Rv from the National Center for Biotechnology Information (NCBI). In addition, the sequence was analyzed with multiple sequence alignment (Clustal IW) to identify the mutation. Our result suggests that the evaluation of genes in M. tuberculosis isolates revealed sequence variation in ripA regions (Ala701Gly). Understanding these mutations implies an evaluation of antibiotic-resistant. Furthermore, this information implies local diagnostic and treatment guidelines to cell-wall targeting antibiotics.</description><identifier>ISSN: 1742-6588</identifier><identifier>EISSN: 1742-6596</identifier><identifier>DOI: 10.1088/1742-6596/1918/5/052014</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Antibiotics ; Deoxyribonucleic acid ; DNA ; Drug resistance ; Gene sequencing ; Mutation ; Public health ; Tuberculosis</subject><ispartof>Journal of physics. Conference series, 2021-06, Vol.1918 (5), p.52014</ispartof><rights>Published under licence by IOP Publishing Ltd</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2764-d43fbf015f81ae375a4bab34b6f3b42e0a178f7dd982b4d4c70a996bc58171f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1742-6596/1918/5/052014/pdf$$EPDF$$P50$$Giop$$Hfree_for_read</linktopdf><link.rule.ids>314,776,780,27903,27904,38847,38869,53818,53845</link.rule.ids></links><search><creatorcontrib>Koentjoro, M P</creatorcontrib><creatorcontrib>Rahayu, D S</creatorcontrib><creatorcontrib>Donastin, A</creatorcontrib><creatorcontrib>Prasetyo, E N</creatorcontrib><title>Evaluation of Mycobacterium tuberculosis ripA gene to detect antibiotic resistance</title><title>Journal of physics. Conference series</title><addtitle>J. Phys.: Conf. Ser</addtitle><description>Mycobacterium tuberculosis
infection has remained a public health threat in Indonesia. This infection is complicated by the antibiotic-resistant of
M. tuberculosis
strains. The common essential in resistance comes from a mutation in genomic DNA. ripA gene is one of the regions critical in the replication and persistence of
M. tuberculosis
in their resistance. This gene has responsible for cell wall polymer peptidoglycan. The objective of this research was to evaluate the ripA gene in antibiotic resistance. This is to investigate and compare the ripA gene sequences of
M. tuberculosis
at an unprecedented rate. A total of five specimens of
M. tuberculosis
were isolated from tuberculosis patients with rifampicin resistance. The ripA gene from
M. tuberculosis
was isolated and amplified using a design primer for ripA N-terminal domain of peptidoglycan hydrolase. Further, ripA gene was analyzed using the Sanger method sequencing. The data were analyzed and compared using
M. tuberculosis
H37Rv from the National Center for Biotechnology Information (NCBI). In addition, the sequence was analyzed with multiple sequence alignment (Clustal IW) to identify the mutation. Our result suggests that the evaluation of genes in M. tuberculosis isolates revealed sequence variation in ripA regions (Ala701Gly). Understanding these mutations implies an evaluation of antibiotic-resistant. Furthermore, this information implies local diagnostic and treatment guidelines to cell-wall targeting antibiotics.</description><subject>Antibiotics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>Gene sequencing</subject><subject>Mutation</subject><subject>Public health</subject><subject>Tuberculosis</subject><issn>1742-6588</issn><issn>1742-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>O3W</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkF1LwzAUhoMoOKe_wYDXtUmTNOnlGPMDJoLodUjSRDK2piapsH9vS2Veem7OgfN-wAPALUb3GAlRYk6romZNXeIGi5KViFUI0zOwOH3OT7cQl-AqpR1CZBy-AG-bb7UfVPahg8HBl6MJWplsox8OMA_aRjPsQ_IJRt-v4KftLMwBtjZbk6Hqstc-ZG9gtKMoq87Ya3Dh1D7Zm9-9BB8Pm_f1U7F9fXxer7aFqXhNi5YSpx3CzAmsLOFMUa00obp2RNPKIoW5cLxtG1Fp2lLDkWqaWhsmMMeuIUtwN-f2MXwNNmW5C0PsxkpZMYo4RxUlo4rPKhNDStE62Ud_UPEoMZITQDmhkRMmOQGUTM4ARyeZnT70f9H_uX4AeCBzSQ</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Koentjoro, M P</creator><creator>Rahayu, D S</creator><creator>Donastin, A</creator><creator>Prasetyo, E N</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>L7M</scope><scope>P5Z</scope><scope>P62</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20210601</creationdate><title>Evaluation of Mycobacterium tuberculosis ripA gene to detect antibiotic resistance</title><author>Koentjoro, M P ; Rahayu, D S ; Donastin, A ; Prasetyo, E N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2764-d43fbf015f81ae375a4bab34b6f3b42e0a178f7dd982b4d4c70a996bc58171f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibiotics</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Gene sequencing</topic><topic>Mutation</topic><topic>Public health</topic><topic>Tuberculosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koentjoro, M P</creatorcontrib><creatorcontrib>Rahayu, D S</creatorcontrib><creatorcontrib>Donastin, A</creatorcontrib><creatorcontrib>Prasetyo, E N</creatorcontrib><collection>IOP Publishing Free Content</collection><collection>IOPscience (Open Access)</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of physics. Conference series</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koentjoro, M P</au><au>Rahayu, D S</au><au>Donastin, A</au><au>Prasetyo, E N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Mycobacterium tuberculosis ripA gene to detect antibiotic resistance</atitle><jtitle>Journal of physics. Conference series</jtitle><addtitle>J. Phys.: Conf. Ser</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>1918</volume><issue>5</issue><spage>52014</spage><pages>52014-</pages><issn>1742-6588</issn><eissn>1742-6596</eissn><abstract>Mycobacterium tuberculosis
infection has remained a public health threat in Indonesia. This infection is complicated by the antibiotic-resistant of
M. tuberculosis
strains. The common essential in resistance comes from a mutation in genomic DNA. ripA gene is one of the regions critical in the replication and persistence of
M. tuberculosis
in their resistance. This gene has responsible for cell wall polymer peptidoglycan. The objective of this research was to evaluate the ripA gene in antibiotic resistance. This is to investigate and compare the ripA gene sequences of
M. tuberculosis
at an unprecedented rate. A total of five specimens of
M. tuberculosis
were isolated from tuberculosis patients with rifampicin resistance. The ripA gene from
M. tuberculosis
was isolated and amplified using a design primer for ripA N-terminal domain of peptidoglycan hydrolase. Further, ripA gene was analyzed using the Sanger method sequencing. The data were analyzed and compared using
M. tuberculosis
H37Rv from the National Center for Biotechnology Information (NCBI). In addition, the sequence was analyzed with multiple sequence alignment (Clustal IW) to identify the mutation. Our result suggests that the evaluation of genes in M. tuberculosis isolates revealed sequence variation in ripA regions (Ala701Gly). Understanding these mutations implies an evaluation of antibiotic-resistant. Furthermore, this information implies local diagnostic and treatment guidelines to cell-wall targeting antibiotics.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1742-6596/1918/5/052014</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Deoxyribonucleic acid DNA Drug resistance Gene sequencing Mutation Public health Tuberculosis |
| title | Evaluation of Mycobacterium tuberculosis ripA gene to detect antibiotic resistance |
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