Effects of Different Ammonia Levels on Tribenuron Methyl Toxicity in Daphnia magna
The present study investigates the toxicity of the herbicide tribenuron methyl (TBM) as an anthropogenic agent and ammonia as an abiotic factor on Daphnia magna at environmentally relevant concentrations. These stressors may coexist in surface waters in agricultural regions. To achieve this objectiv...
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| Veröffentlicht in: | Archives of environmental contamination and toxicology 2021-07, Vol.81 (1), p.46-57 |
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| creator | Başalan Över, Sevgi Guven, Celal Taskin, Eylem Çakmak, Arif Piner Benli, Petek Sevgiler, Yusuf |
| description | The present study investigates the toxicity of the herbicide tribenuron methyl (TBM) as an anthropogenic agent and ammonia as an abiotic factor on
Daphnia magna
at environmentally relevant concentrations. These stressors may coexist in surface waters in agricultural regions. To achieve this objective,
D. magna
were exposed to TBM at a nominal concentration of 0.81 μg/L in association with a low ammonia (LA) concentration of 0.65 mg/L and a high ammonia (HA) concentration of 1.61 mg/L in acute toxicity tests of 96-h duration and chronic toxicity tests of 21-day duration. The
D. magna
also were exposed to TBM, HA, and LA singly. The
D. magna
were analysed for various biomarkers of sublethal toxicity. Glutathione peroxidase (GPx), glutathione
S
-transferase (GST), cholinesterase (ChE) enzyme activities, and levels of thiobarbituric acid reactive substances (TBARS) and total protein were determined spectrophotometrically. Mitochondrial membrane potential (MMP) was analysed by microscopy with fluorescence staining. Cytochrome c and 5′ AMP-activated protein kinase (AMPK) were analysed by Western blotting. Morphometric properties were examined microscopically. This is the first study in which AMPK, an indicator of intracellular energy, was measured in
D. magna
. GST and ChE enzyme activities and TBARS and total protein levels did not change during acute exposures (i.e., 96 h) in all treatments. GPx activity increased in
D. magna
from the HA + TBM treatment compared with single-exposure groups. The level of cytochrome c protein was elevated in
D. magna
from the LA and LA + TBM treatments. AMPK protein levels increased in all treatments with daphnids, except in the LA group. MMP was depolarised in
D. magna
from all treatments, whereas the most notable change was observed in HA + TBM mixture group in chronic exposures. The results show that GST and ChE may not be sensitive biomarkers for evaluating the sublethal toxic effects to
D. magna
exposed to environmentally relevant concentrations of ammonia and TBM. Acute and chronic exposure to ammonia and TBM probably caused an energetic crisis in
D. magna
. Therefore, AMPK and MMP are promising biomarkers for these toxicants. |
| doi_str_mv | 10.1007/s00244-021-00841-3 |
| format | Article |
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Daphnia magna
at environmentally relevant concentrations. These stressors may coexist in surface waters in agricultural regions. To achieve this objective,
D. magna
were exposed to TBM at a nominal concentration of 0.81 μg/L in association with a low ammonia (LA) concentration of 0.65 mg/L and a high ammonia (HA) concentration of 1.61 mg/L in acute toxicity tests of 96-h duration and chronic toxicity tests of 21-day duration. The
D. magna
also were exposed to TBM, HA, and LA singly. The
D. magna
were analysed for various biomarkers of sublethal toxicity. Glutathione peroxidase (GPx), glutathione
S
-transferase (GST), cholinesterase (ChE) enzyme activities, and levels of thiobarbituric acid reactive substances (TBARS) and total protein were determined spectrophotometrically. Mitochondrial membrane potential (MMP) was analysed by microscopy with fluorescence staining. Cytochrome c and 5′ AMP-activated protein kinase (AMPK) were analysed by Western blotting. Morphometric properties were examined microscopically. This is the first study in which AMPK, an indicator of intracellular energy, was measured in
D. magna
. GST and ChE enzyme activities and TBARS and total protein levels did not change during acute exposures (i.e., 96 h) in all treatments. GPx activity increased in
D. magna
from the HA + TBM treatment compared with single-exposure groups. The level of cytochrome c protein was elevated in
D. magna
from the LA and LA + TBM treatments. AMPK protein levels increased in all treatments with daphnids, except in the LA group. MMP was depolarised in
D. magna
from all treatments, whereas the most notable change was observed in HA + TBM mixture group in chronic exposures. The results show that GST and ChE may not be sensitive biomarkers for evaluating the sublethal toxic effects to
D. magna
exposed to environmentally relevant concentrations of ammonia and TBM. Acute and chronic exposure to ammonia and TBM probably caused an energetic crisis in
D. magna
. Therefore, AMPK and MMP are promising biomarkers for these toxicants.</description><identifier>ISSN: 0090-4341</identifier><identifier>EISSN: 1432-0703</identifier><identifier>DOI: 10.1007/s00244-021-00841-3</identifier><identifier>PMID: 33864096</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Abiotic factors ; Acute toxicity ; Ammonia ; AMP-activated protein kinase ; Anthropogenic factors ; Biomarkers ; C protein ; Cholinesterase ; Chronic exposure ; Chronic toxicity ; Cytochrome ; Cytochrome c ; Cytochromes ; Daphnia magna ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Enzymatic activity ; Enzymes ; Exposure ; Fluorescence ; Glutathione ; Glutathione peroxidase ; Glutathione transferase ; Herbicides ; Kinases ; Membrane potential ; Mitochondria ; Monitoring/Environmental Analysis ; Peroxidase ; Pollution ; Proteins ; Sensitivity analysis ; Soil Science & Conservation ; Spectrophotometry ; Surface water ; Thiobarbituric acid ; Toxicants ; Toxicity ; Toxicity testing ; Waterways ; Western blotting</subject><ispartof>Archives of environmental contamination and toxicology, 2021-07, Vol.81 (1), p.46-57</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-c5a7a2ad9202ba919d2567cc6a68f0ad8949f11ef45e358b0b648ba207b42d373</citedby><cites>FETCH-LOGICAL-c375t-c5a7a2ad9202ba919d2567cc6a68f0ad8949f11ef45e358b0b648ba207b42d373</cites><orcidid>0000-0001-8172-4980 ; 0000-0003-0499-7787 ; 0000-0002-4450-7124 ; 0000-0002-4373-2389 ; 0000-0002-8646-3553 ; 0000-0003-2324-9047</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00244-021-00841-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00244-021-00841-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33864096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Başalan Över, Sevgi</creatorcontrib><creatorcontrib>Guven, Celal</creatorcontrib><creatorcontrib>Taskin, Eylem</creatorcontrib><creatorcontrib>Çakmak, Arif</creatorcontrib><creatorcontrib>Piner Benli, Petek</creatorcontrib><creatorcontrib>Sevgiler, Yusuf</creatorcontrib><title>Effects of Different Ammonia Levels on Tribenuron Methyl Toxicity in Daphnia magna</title><title>Archives of environmental contamination and toxicology</title><addtitle>Arch Environ Contam Toxicol</addtitle><addtitle>Arch Environ Contam Toxicol</addtitle><description>The present study investigates the toxicity of the herbicide tribenuron methyl (TBM) as an anthropogenic agent and ammonia as an abiotic factor on
Daphnia magna
at environmentally relevant concentrations. These stressors may coexist in surface waters in agricultural regions. To achieve this objective,
D. magna
were exposed to TBM at a nominal concentration of 0.81 μg/L in association with a low ammonia (LA) concentration of 0.65 mg/L and a high ammonia (HA) concentration of 1.61 mg/L in acute toxicity tests of 96-h duration and chronic toxicity tests of 21-day duration. The
D. magna
also were exposed to TBM, HA, and LA singly. The
D. magna
were analysed for various biomarkers of sublethal toxicity. Glutathione peroxidase (GPx), glutathione
S
-transferase (GST), cholinesterase (ChE) enzyme activities, and levels of thiobarbituric acid reactive substances (TBARS) and total protein were determined spectrophotometrically. Mitochondrial membrane potential (MMP) was analysed by microscopy with fluorescence staining. Cytochrome c and 5′ AMP-activated protein kinase (AMPK) were analysed by Western blotting. Morphometric properties were examined microscopically. This is the first study in which AMPK, an indicator of intracellular energy, was measured in
D. magna
. GST and ChE enzyme activities and TBARS and total protein levels did not change during acute exposures (i.e., 96 h) in all treatments. GPx activity increased in
D. magna
from the HA + TBM treatment compared with single-exposure groups. The level of cytochrome c protein was elevated in
D. magna
from the LA and LA + TBM treatments. AMPK protein levels increased in all treatments with daphnids, except in the LA group. MMP was depolarised in
D. magna
from all treatments, whereas the most notable change was observed in HA + TBM mixture group in chronic exposures. The results show that GST and ChE may not be sensitive biomarkers for evaluating the sublethal toxic effects to
D. magna
exposed to environmentally relevant concentrations of ammonia and TBM. Acute and chronic exposure to ammonia and TBM probably caused an energetic crisis in
D. magna
. Therefore, AMPK and MMP are promising biomarkers for these toxicants.</description><subject>Abiotic factors</subject><subject>Acute toxicity</subject><subject>Ammonia</subject><subject>AMP-activated protein kinase</subject><subject>Anthropogenic factors</subject><subject>Biomarkers</subject><subject>C protein</subject><subject>Cholinesterase</subject><subject>Chronic exposure</subject><subject>Chronic toxicity</subject><subject>Cytochrome</subject><subject>Cytochrome c</subject><subject>Cytochromes</subject><subject>Daphnia magna</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Exposure</subject><subject>Fluorescence</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Glutathione transferase</subject><subject>Herbicides</subject><subject>Kinases</subject><subject>Membrane potential</subject><subject>Mitochondria</subject><subject>Monitoring/Environmental Analysis</subject><subject>Peroxidase</subject><subject>Pollution</subject><subject>Proteins</subject><subject>Sensitivity analysis</subject><subject>Soil Science & Conservation</subject><subject>Spectrophotometry</subject><subject>Surface water</subject><subject>Thiobarbituric acid</subject><subject>Toxicants</subject><subject>Toxicity</subject><subject>Toxicity testing</subject><subject>Waterways</subject><subject>Western 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of Different Ammonia Levels on Tribenuron Methyl Toxicity in Daphnia magna</title><author>Başalan Över, Sevgi ; Guven, Celal ; Taskin, Eylem ; Çakmak, Arif ; Piner Benli, Petek ; Sevgiler, Yusuf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-c5a7a2ad9202ba919d2567cc6a68f0ad8949f11ef45e358b0b648ba207b42d373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Abiotic factors</topic><topic>Acute toxicity</topic><topic>Ammonia</topic><topic>AMP-activated protein kinase</topic><topic>Anthropogenic factors</topic><topic>Biomarkers</topic><topic>C protein</topic><topic>Cholinesterase</topic><topic>Chronic exposure</topic><topic>Chronic toxicity</topic><topic>Cytochrome</topic><topic>Cytochrome c</topic><topic>Cytochromes</topic><topic>Daphnia magna</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Exposure</topic><topic>Fluorescence</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Glutathione transferase</topic><topic>Herbicides</topic><topic>Kinases</topic><topic>Membrane potential</topic><topic>Mitochondria</topic><topic>Monitoring/Environmental Analysis</topic><topic>Peroxidase</topic><topic>Pollution</topic><topic>Proteins</topic><topic>Sensitivity analysis</topic><topic>Soil Science & Conservation</topic><topic>Spectrophotometry</topic><topic>Surface water</topic><topic>Thiobarbituric acid</topic><topic>Toxicants</topic><topic>Toxicity</topic><topic>Toxicity testing</topic><topic>Waterways</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Başalan Över, Sevgi</creatorcontrib><creatorcontrib>Guven, Celal</creatorcontrib><creatorcontrib>Taskin, 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Toxicol</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>81</volume><issue>1</issue><spage>46</spage><epage>57</epage><pages>46-57</pages><issn>0090-4341</issn><eissn>1432-0703</eissn><abstract>The present study investigates the toxicity of the herbicide tribenuron methyl (TBM) as an anthropogenic agent and ammonia as an abiotic factor on
Daphnia magna
at environmentally relevant concentrations. These stressors may coexist in surface waters in agricultural regions. To achieve this objective,
D. magna
were exposed to TBM at a nominal concentration of 0.81 μg/L in association with a low ammonia (LA) concentration of 0.65 mg/L and a high ammonia (HA) concentration of 1.61 mg/L in acute toxicity tests of 96-h duration and chronic toxicity tests of 21-day duration. The
D. magna
also were exposed to TBM, HA, and LA singly. The
D. magna
were analysed for various biomarkers of sublethal toxicity. Glutathione peroxidase (GPx), glutathione
S
-transferase (GST), cholinesterase (ChE) enzyme activities, and levels of thiobarbituric acid reactive substances (TBARS) and total protein were determined spectrophotometrically. Mitochondrial membrane potential (MMP) was analysed by microscopy with fluorescence staining. Cytochrome c and 5′ AMP-activated protein kinase (AMPK) were analysed by Western blotting. Morphometric properties were examined microscopically. This is the first study in which AMPK, an indicator of intracellular energy, was measured in
D. magna
. GST and ChE enzyme activities and TBARS and total protein levels did not change during acute exposures (i.e., 96 h) in all treatments. GPx activity increased in
D. magna
from the HA + TBM treatment compared with single-exposure groups. The level of cytochrome c protein was elevated in
D. magna
from the LA and LA + TBM treatments. AMPK protein levels increased in all treatments with daphnids, except in the LA group. MMP was depolarised in
D. magna
from all treatments, whereas the most notable change was observed in HA + TBM mixture group in chronic exposures. The results show that GST and ChE may not be sensitive biomarkers for evaluating the sublethal toxic effects to
D. magna
exposed to environmentally relevant concentrations of ammonia and TBM. Acute and chronic exposure to ammonia and TBM probably caused an energetic crisis in
D. magna
. Therefore, AMPK and MMP are promising biomarkers for these toxicants.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33864096</pmid><doi>10.1007/s00244-021-00841-3</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8172-4980</orcidid><orcidid>https://orcid.org/0000-0003-0499-7787</orcidid><orcidid>https://orcid.org/0000-0002-4450-7124</orcidid><orcidid>https://orcid.org/0000-0002-4373-2389</orcidid><orcidid>https://orcid.org/0000-0002-8646-3553</orcidid><orcidid>https://orcid.org/0000-0003-2324-9047</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Archives of environmental contamination and toxicology, 2021-07, Vol.81 (1), p.46-57 |
| issn | 0090-4341 1432-0703 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Abiotic factors Acute toxicity Ammonia AMP-activated protein kinase Anthropogenic factors Biomarkers C protein Cholinesterase Chronic exposure Chronic toxicity Cytochrome Cytochrome c Cytochromes Daphnia magna Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Enzymatic activity Enzymes Exposure Fluorescence Glutathione Glutathione peroxidase Glutathione transferase Herbicides Kinases Membrane potential Mitochondria Monitoring/Environmental Analysis Peroxidase Pollution Proteins Sensitivity analysis Soil Science & Conservation Spectrophotometry Surface water Thiobarbituric acid Toxicants Toxicity Toxicity testing Waterways Western blotting |
| title | Effects of Different Ammonia Levels on Tribenuron Methyl Toxicity in Daphnia magna |
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