The effectiveness of different culture media on the growth and proliferation of Acanthamoeba T4 genotype
Acanthamoeba spp. is one of the free-living amoebas in the environment that many studies focused on. This parasite is of veterinary and public health importance. The present study aimed to evaluate several available media for Acanthamoeba spp. culture to find a suitable axenic liquid medium. In the...
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| Veröffentlicht in: | Comparative clinical pathology 2021-06, Vol.30 (3), p.515-518 |
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| creator | Hezarjaribi, Hajar Ziaei Dadimoghaddam, Yousef Saravi, Karan Hassanjani Faridnia, Roghiyeh Kalani, Hamed Fakhar, Mahdi Jorjani, Ogholniaz Saberi, Reza Tolouei, Elahe Bazgir, Mahdi |
| description | Acanthamoeba
spp. is one of the free-living amoebas in the environment that many studies focused on. This parasite is of veterinary and public health importance. The present study aimed to evaluate several available media for
Acanthamoeba
spp. culture to find a suitable axenic liquid medium. In the present study four culture media, including TYM, RPMI-1640, and hydatid cyst fluid (HCF) as well as non-nutrient agar (NNA) as the control group, were examined to evaluate the proliferation of
Acanthamoeba
T4 genotype at 24, 48, and 72 h. Based on our results, at 24 and 48 h, the highest number of cysts was observed in TYM medium and the lowest in NNA medium. Moreover, at 72 h, the highest and lowest number of cysts was detected in the HCF and NNA media, respectively. In the present study, the best medium for proliferation of the parasite in the short term was TYM. Furthermore, the parasite proliferated continuously in a considerable number in HCF medium, and this medium can be used if continuative examinations are needed on the parasite. |
| doi_str_mv | 10.1007/s00580-021-03245-w |
| format | Article |
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spp. is one of the free-living amoebas in the environment that many studies focused on. This parasite is of veterinary and public health importance. The present study aimed to evaluate several available media for
Acanthamoeba
spp. culture to find a suitable axenic liquid medium. In the present study four culture media, including TYM, RPMI-1640, and hydatid cyst fluid (HCF) as well as non-nutrient agar (NNA) as the control group, were examined to evaluate the proliferation of
Acanthamoeba
T4 genotype at 24, 48, and 72 h. Based on our results, at 24 and 48 h, the highest number of cysts was observed in TYM medium and the lowest in NNA medium. Moreover, at 72 h, the highest and lowest number of cysts was detected in the HCF and NNA media, respectively. In the present study, the best medium for proliferation of the parasite in the short term was TYM. Furthermore, the parasite proliferated continuously in a considerable number in HCF medium, and this medium can be used if continuative examinations are needed on the parasite.</description><identifier>ISSN: 1618-5641</identifier><identifier>EISSN: 1618-565X</identifier><identifier>DOI: 10.1007/s00580-021-03245-w</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Acanthamoeba ; Amoeba ; Culture media ; Cysts ; Genotypes ; Germfree ; Hematology ; Medicine ; Medicine & Public Health ; Oncology ; Original Article ; Parasites ; Pathology ; Public health</subject><ispartof>Comparative clinical pathology, 2021-06, Vol.30 (3), p.515-518</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c185w-964ada3933d97f68dc00f22de6fd95471ca156fa5864dc78d74c1bc4e182658b3</cites><orcidid>0000-0001-8325-0467</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00580-021-03245-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00580-021-03245-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Hezarjaribi, Hajar Ziaei</creatorcontrib><creatorcontrib>Dadimoghaddam, Yousef</creatorcontrib><creatorcontrib>Saravi, Karan Hassanjani</creatorcontrib><creatorcontrib>Faridnia, Roghiyeh</creatorcontrib><creatorcontrib>Kalani, Hamed</creatorcontrib><creatorcontrib>Fakhar, Mahdi</creatorcontrib><creatorcontrib>Jorjani, Ogholniaz</creatorcontrib><creatorcontrib>Saberi, Reza</creatorcontrib><creatorcontrib>Tolouei, Elahe</creatorcontrib><creatorcontrib>Bazgir, Mahdi</creatorcontrib><title>The effectiveness of different culture media on the growth and proliferation of Acanthamoeba T4 genotype</title><title>Comparative clinical pathology</title><addtitle>Comp Clin Pathol</addtitle><description>Acanthamoeba
spp. is one of the free-living amoebas in the environment that many studies focused on. This parasite is of veterinary and public health importance. The present study aimed to evaluate several available media for
Acanthamoeba
spp. culture to find a suitable axenic liquid medium. In the present study four culture media, including TYM, RPMI-1640, and hydatid cyst fluid (HCF) as well as non-nutrient agar (NNA) as the control group, were examined to evaluate the proliferation of
Acanthamoeba
T4 genotype at 24, 48, and 72 h. Based on our results, at 24 and 48 h, the highest number of cysts was observed in TYM medium and the lowest in NNA medium. Moreover, at 72 h, the highest and lowest number of cysts was detected in the HCF and NNA media, respectively. In the present study, the best medium for proliferation of the parasite in the short term was TYM. Furthermore, the parasite proliferated continuously in a considerable number in HCF medium, and this medium can be used if continuative examinations are needed on the parasite.</description><subject>Acanthamoeba</subject><subject>Amoeba</subject><subject>Culture media</subject><subject>Cysts</subject><subject>Genotypes</subject><subject>Germfree</subject><subject>Hematology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Parasites</subject><subject>Pathology</subject><subject>Public health</subject><issn>1618-5641</issn><issn>1618-565X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LAzEQhhdRsFb_gKeA59Vk87HZYyl-QcFLBW8hTSbdLW22JqlL_73RFb15mmHmed8Z3qK4JviWYFzfRYy5xCWuSIlpxXg5nBQTIogsueBvp789I-fFRYwbjAmXlE6KdtkCAufApO4DPMSIeodslycBfELmsE2HAGgHttOo9yhlfh36IbVIe4v2od92mdWpy8ssnRntU6t3Paw0WjK0Bt-n4x4uizOntxGufuq0eH24X86fysXL4_N8tigNkXwoG8G01bSh1Da1E9IajF1VWRDONpzVxGjChdNcCmZNLW3NDFkZBkRWgssVnRY3o2_-7P0AMalNfwg-n1QVp1I2omIkU9VImdDHGMCpfeh2OhwVweorUTUmqnKi6jtRNWQRHUUxw34N4c_6H9Un0796uw</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Hezarjaribi, Hajar Ziaei</creator><creator>Dadimoghaddam, Yousef</creator><creator>Saravi, Karan Hassanjani</creator><creator>Faridnia, Roghiyeh</creator><creator>Kalani, Hamed</creator><creator>Fakhar, Mahdi</creator><creator>Jorjani, Ogholniaz</creator><creator>Saberi, Reza</creator><creator>Tolouei, Elahe</creator><creator>Bazgir, Mahdi</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0001-8325-0467</orcidid></search><sort><creationdate>20210601</creationdate><title>The effectiveness of different culture media on the growth and proliferation of Acanthamoeba T4 genotype</title><author>Hezarjaribi, Hajar Ziaei ; 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spp. is one of the free-living amoebas in the environment that many studies focused on. This parasite is of veterinary and public health importance. The present study aimed to evaluate several available media for
Acanthamoeba
spp. culture to find a suitable axenic liquid medium. In the present study four culture media, including TYM, RPMI-1640, and hydatid cyst fluid (HCF) as well as non-nutrient agar (NNA) as the control group, were examined to evaluate the proliferation of
Acanthamoeba
T4 genotype at 24, 48, and 72 h. Based on our results, at 24 and 48 h, the highest number of cysts was observed in TYM medium and the lowest in NNA medium. Moreover, at 72 h, the highest and lowest number of cysts was detected in the HCF and NNA media, respectively. In the present study, the best medium for proliferation of the parasite in the short term was TYM. Furthermore, the parasite proliferated continuously in a considerable number in HCF medium, and this medium can be used if continuative examinations are needed on the parasite.</abstract><cop>London</cop><pub>Springer London</pub><doi>10.1007/s00580-021-03245-w</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0001-8325-0467</orcidid></addata></record> |
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| subjects | Acanthamoeba Amoeba Culture media Cysts Genotypes Germfree Hematology Medicine Medicine & Public Health Oncology Original Article Parasites Pathology Public health |
| title | The effectiveness of different culture media on the growth and proliferation of Acanthamoeba T4 genotype |
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