The genome of the European estuarine calanoid copepod Eurytemora affinis: Potential use in molecular ecotoxicology

In this study, we sequenced and assembled the genome of a European estuarine calanoid copepod using Oxford Nanopore PromethION and Illumina HiSeq 2500 platforms. The length of the assembled genome was 776.1 Mb with N50 = 474.9 kb (BUSCO 85.9%), and the genome consisted of 2473 contigs. A total of 18,014 genes were annotated and orthologous gene clusters were analyzed in comparison to other copepods. In addition, genome-wide identification of cytochrome P450s, glutathione S-transferases, and ATP-binding cassette transporters in E. affinis was performed to determine gene repertoire of these detoxification-related gene families. Results revealed the presence of species-specific gene inventories, indicating that these gene families have evolved through species-specific gene loss/expansion processes, possibly due to adaptation to different environmental stressors. Our study provides a new inventory of the European estuarine calanoid copepod E. affinis genome with emphasis on phase I, II, and III detoxification systems. •The length of the assembled genome was 776.1 Mb with N50 = 474.9 kb (BUSCO 85.9%).•A total of 18,014 genes were annotated in European calanoid copepod Eurytemora affinis.•Cytochrome P450s, glutathione S-transferases, and ATP-binding cassette proteins genes were fully identified.