Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella
Members of the Raoultella planticola species isolated from soils contaminated with chemical waste were able to use 2,4-dichlorophenoxyacetic/2,4,5-trichlorophenoxyacetic acid as the sole source of carbon and energy. It was found that cells of strains 33-4ch, 36D, and 36T contained plasmids that were...
Gespeichert in:
| Veröffentlicht in: | Applied biochemistry and microbiology 2021-05, Vol.57 (3), p.335-343 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 343 |
|---|---|
| container_issue | 3 |
| container_start_page | 335 |
| container_title | Applied biochemistry and microbiology |
| container_volume | 57 |
| creator | Zharikova, N. V. Iasakov, T. R. Zhurenko, E. I. Korobov, V. V. Markusheva, T. V. |
| description | Members of the
Raoultella planticola
species isolated from soils contaminated with chemical waste were able to use 2,4-dichlorophenoxyacetic/2,4,5-trichlorophenoxyacetic acid as the sole source of carbon and energy. It was found that cells of strains 33-4ch, 36D, and 36T contained plasmids that were designated as pRP33-4ch, pRP36D and pRP36T, respectively. Restriction fragment length polymorphism (RFLP) analysis showed that pRP33-4ch and pRP36T were probably the same plasmid, while pRP36D contained additional fragments in the restriction profiles. Plasmid elimination showed that the genes for the degradation of chlorophenoxyacetic acids have extrachromosomal localization. Polymerase chain reaction (PCR) demonstrated the absence in the genomes of the studied strains of known initiation genes (
tfdA
and
tftA
) for the conversion of chlorophenoxyacetic acids. The identified intermediate metabolites (chlorophenoxyacetic and phenoxyacetic acids and 2-keto-3-methylmuconic semialdehyde) indicated that the strains 33-4ch, 36D, and 36T perform complete dechlorination of the aromatic ring of the substrate with its subsequent meta-cleavage. |
| doi_str_mv | 10.1134/S0003683821030157 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2536819300</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2536819300</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-27cbd8f8a08a1c39e6f752e02a98ff401349cc112582edf844e80a5ce3189ebc3</originalsourceid><addsrcrecordid>eNp1kM1Lw0AQxRdRsEb_AG8Bz9H9SNLdY61ahYLiB3gL081sm5Jm6-4G7H_vhioexNMwzPu94T1Czhm9ZEzkVy-UUlFKITmjgrJifEBGrKQyE5Tnh2Q0nLPhfkxOvF_HVZVSjcj7Uwt-09Q-tSYNK0ynq9Y6u11hZz93oDE0Opvopk5vcOmghtDYbtBegw7oGvjhZtj1Pn0G27cB2xZOyZGB1uPZ90zI293t6_Q-mz_OHqaTeaZFIULGx3pRSyOBSmBaKCzNuOBIOShpTE5jNqU1Y7yQHGsj8xwlhUKjYFLhQouEXOx9t85-9OhDtba96-LLihexEaZEjJ4QtldpZ713aKqtazbgdhWj1VBg9afAyPA946O2W6L7df4f-gKPM3G6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2536819300</pqid></control><display><type>article</type><title>Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella</title><source>SpringerLink Journals - AutoHoldings</source><creator>Zharikova, N. V. ; Iasakov, T. R. ; Zhurenko, E. I. ; Korobov, V. V. ; Markusheva, T. V.</creator><creatorcontrib>Zharikova, N. V. ; Iasakov, T. R. ; Zhurenko, E. I. ; Korobov, V. V. ; Markusheva, T. V.</creatorcontrib><description>Members of the
Raoultella planticola
species isolated from soils contaminated with chemical waste were able to use 2,4-dichlorophenoxyacetic/2,4,5-trichlorophenoxyacetic acid as the sole source of carbon and energy. It was found that cells of strains 33-4ch, 36D, and 36T contained plasmids that were designated as pRP33-4ch, pRP36D and pRP36T, respectively. Restriction fragment length polymorphism (RFLP) analysis showed that pRP33-4ch and pRP36T were probably the same plasmid, while pRP36D contained additional fragments in the restriction profiles. Plasmid elimination showed that the genes for the degradation of chlorophenoxyacetic acids have extrachromosomal localization. Polymerase chain reaction (PCR) demonstrated the absence in the genomes of the studied strains of known initiation genes (
tfdA
and
tftA
) for the conversion of chlorophenoxyacetic acids. The identified intermediate metabolites (chlorophenoxyacetic and phenoxyacetic acids and 2-keto-3-methylmuconic semialdehyde) indicated that the strains 33-4ch, 36D, and 36T perform complete dechlorination of the aromatic ring of the substrate with its subsequent meta-cleavage.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683821030157</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Acids ; Aromatic compounds ; Biochemistry ; Biodegradation ; Biomedical and Life Sciences ; Chemical wastes ; Chlorophenoxyacetic acids ; Dechlorination ; Degradation ; Genes ; Genomes ; Life Sciences ; Localization ; Medical Microbiology ; Metabolites ; Microbiology ; Plasmids ; Polymerase chain reaction ; Polymorphism ; Raoultella ; Restriction fragment length polymorphism ; Soil chemistry ; Soil contamination ; Soil pollution ; Strains (organisms) ; Substrates</subject><ispartof>Applied biochemistry and microbiology, 2021-05, Vol.57 (3), p.335-343</ispartof><rights>Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 3, pp. 335–343. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 3, pp. 235–244.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-27cbd8f8a08a1c39e6f752e02a98ff401349cc112582edf844e80a5ce3189ebc3</citedby><cites>FETCH-LOGICAL-c353t-27cbd8f8a08a1c39e6f752e02a98ff401349cc112582edf844e80a5ce3189ebc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683821030157$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683821030157$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Zharikova, N. V.</creatorcontrib><creatorcontrib>Iasakov, T. R.</creatorcontrib><creatorcontrib>Zhurenko, E. I.</creatorcontrib><creatorcontrib>Korobov, V. V.</creatorcontrib><creatorcontrib>Markusheva, T. V.</creatorcontrib><title>Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Members of the
Raoultella planticola
species isolated from soils contaminated with chemical waste were able to use 2,4-dichlorophenoxyacetic/2,4,5-trichlorophenoxyacetic acid as the sole source of carbon and energy. It was found that cells of strains 33-4ch, 36D, and 36T contained plasmids that were designated as pRP33-4ch, pRP36D and pRP36T, respectively. Restriction fragment length polymorphism (RFLP) analysis showed that pRP33-4ch and pRP36T were probably the same plasmid, while pRP36D contained additional fragments in the restriction profiles. Plasmid elimination showed that the genes for the degradation of chlorophenoxyacetic acids have extrachromosomal localization. Polymerase chain reaction (PCR) demonstrated the absence in the genomes of the studied strains of known initiation genes (
tfdA
and
tftA
) for the conversion of chlorophenoxyacetic acids. The identified intermediate metabolites (chlorophenoxyacetic and phenoxyacetic acids and 2-keto-3-methylmuconic semialdehyde) indicated that the strains 33-4ch, 36D, and 36T perform complete dechlorination of the aromatic ring of the substrate with its subsequent meta-cleavage.</description><subject>Acids</subject><subject>Aromatic compounds</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Chemical wastes</subject><subject>Chlorophenoxyacetic acids</subject><subject>Dechlorination</subject><subject>Degradation</subject><subject>Genes</subject><subject>Genomes</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Medical Microbiology</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Raoultella</subject><subject>Restriction fragment length polymorphism</subject><subject>Soil chemistry</subject><subject>Soil contamination</subject><subject>Soil pollution</subject><subject>Strains (organisms)</subject><subject>Substrates</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kM1Lw0AQxRdRsEb_AG8Bz9H9SNLdY61ahYLiB3gL081sm5Jm6-4G7H_vhioexNMwzPu94T1Czhm9ZEzkVy-UUlFKITmjgrJifEBGrKQyE5Tnh2Q0nLPhfkxOvF_HVZVSjcj7Uwt-09Q-tSYNK0ynq9Y6u11hZz93oDE0Opvopk5vcOmghtDYbtBegw7oGvjhZtj1Pn0G27cB2xZOyZGB1uPZ90zI293t6_Q-mz_OHqaTeaZFIULGx3pRSyOBSmBaKCzNuOBIOShpTE5jNqU1Y7yQHGsj8xwlhUKjYFLhQouEXOx9t85-9OhDtba96-LLihexEaZEjJ4QtldpZ713aKqtazbgdhWj1VBg9afAyPA946O2W6L7df4f-gKPM3G6</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Zharikova, N. V.</creator><creator>Iasakov, T. R.</creator><creator>Zhurenko, E. I.</creator><creator>Korobov, V. V.</creator><creator>Markusheva, T. V.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20210501</creationdate><title>Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella</title><author>Zharikova, N. V. ; Iasakov, T. R. ; Zhurenko, E. I. ; Korobov, V. V. ; Markusheva, T. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-27cbd8f8a08a1c39e6f752e02a98ff401349cc112582edf844e80a5ce3189ebc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acids</topic><topic>Aromatic compounds</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Chemical wastes</topic><topic>Chlorophenoxyacetic acids</topic><topic>Dechlorination</topic><topic>Degradation</topic><topic>Genes</topic><topic>Genomes</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Medical Microbiology</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Raoultella</topic><topic>Restriction fragment length polymorphism</topic><topic>Soil chemistry</topic><topic>Soil contamination</topic><topic>Soil pollution</topic><topic>Strains (organisms)</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zharikova, N. V.</creatorcontrib><creatorcontrib>Iasakov, T. R.</creatorcontrib><creatorcontrib>Zhurenko, E. I.</creatorcontrib><creatorcontrib>Korobov, V. V.</creatorcontrib><creatorcontrib>Markusheva, T. V.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zharikova, N. V.</au><au>Iasakov, T. R.</au><au>Zhurenko, E. I.</au><au>Korobov, V. V.</au><au>Markusheva, T. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2021-05-01</date><risdate>2021</risdate><volume>57</volume><issue>3</issue><spage>335</spage><epage>343</epage><pages>335-343</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Members of the
Raoultella planticola
species isolated from soils contaminated with chemical waste were able to use 2,4-dichlorophenoxyacetic/2,4,5-trichlorophenoxyacetic acid as the sole source of carbon and energy. It was found that cells of strains 33-4ch, 36D, and 36T contained plasmids that were designated as pRP33-4ch, pRP36D and pRP36T, respectively. Restriction fragment length polymorphism (RFLP) analysis showed that pRP33-4ch and pRP36T were probably the same plasmid, while pRP36D contained additional fragments in the restriction profiles. Plasmid elimination showed that the genes for the degradation of chlorophenoxyacetic acids have extrachromosomal localization. Polymerase chain reaction (PCR) demonstrated the absence in the genomes of the studied strains of known initiation genes (
tfdA
and
tftA
) for the conversion of chlorophenoxyacetic acids. The identified intermediate metabolites (chlorophenoxyacetic and phenoxyacetic acids and 2-keto-3-methylmuconic semialdehyde) indicated that the strains 33-4ch, 36D, and 36T perform complete dechlorination of the aromatic ring of the substrate with its subsequent meta-cleavage.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683821030157</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-6838 |
| ispartof | Applied biochemistry and microbiology, 2021-05, Vol.57 (3), p.335-343 |
| issn | 0003-6838 1608-3024 |
| language | eng |
| recordid | cdi_proquest_journals_2536819300 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Acids Aromatic compounds Biochemistry Biodegradation Biomedical and Life Sciences Chemical wastes Chlorophenoxyacetic acids Dechlorination Degradation Genes Genomes Life Sciences Localization Medical Microbiology Metabolites Microbiology Plasmids Polymerase chain reaction Polymorphism Raoultella Restriction fragment length polymorphism Soil chemistry Soil contamination Soil pollution Strains (organisms) Substrates |
| title | Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T03%3A46%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasmids%20of%20the%20Chlorophenoxyacetic-Acid%20Degradation%20of%20Bacteria%20of%20the%20Genus%20Raoultella&rft.jtitle=Applied%20biochemistry%20and%20microbiology&rft.au=Zharikova,%20N.%20V.&rft.date=2021-05-01&rft.volume=57&rft.issue=3&rft.spage=335&rft.epage=343&rft.pages=335-343&rft.issn=0003-6838&rft.eissn=1608-3024&rft_id=info:doi/10.1134/S0003683821030157&rft_dat=%3Cproquest_cross%3E2536819300%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2536819300&rft_id=info:pmid/&rfr_iscdi=true |