Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran
One of the mechanisms of Klebsiella pneumoniae and Escherichia coli resistance to β-lactam antibiotics is the production of β-lactamase enzymes. Among these are the AmpC β-lactamases, which confer resistance to a class of antibiotics. However, little is known about the AmpC β-lactamases of K. pneumo...
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| description | One of the mechanisms of
Klebsiella pneumoniae
and
Escherichia coli
resistance to β-lactam antibiotics is the production of β-lactamase enzymes. Among these are the AmpC β-lactamases, which confer resistance to a class of antibiotics. However, little is known about the AmpC β-lactamases of
K. pneumoniae
and
E. coli
clinical isolates in Qazvin, Iran. This study was designed to assess the AmpC β‑lactamases-producing strains and also identify the prevalence of
AmpC
β‑lactamases genes. Antimicrobial susceptibility tests were performed on 435
K. pneumoniae
and
E. coli
isolates using disk diffusion technique. Plasmid-mediated
AmpC
genes were studied using a multiplex PCR assay. The AmpC β-lactamase-producer isolates were studied by employing cefoxitin disk diffusion test, AmpC induction test, AmpC cefoxitin-EDTA test, and boronic acid disk test. Our results showed that of 46 (18.4%) cefoxitin-insensitive
E. coli
isolates, 10 (21.7%) were positive for
AmpC
β-lactamase genes, among them 4 (8.69%) isolates were positive for
bla
DHA
genes and 6 (13%) for
bla
CIT
genes. Of 57 (30.4%) cefoxitin-insensitive
K. pneumoniae
isolates, 10 (17.5%) were positive for AmpC gene with 4 (6.34%) and 6 (9.5%) isolates positive for
bla
DHA
and
bla
CIT
genes, respectively. However, no
MOX
,
ACC
,
FOX
, or
EBC
genes were detected in the isolates. Considering the results of different confirmatory phenotypic tests, the AmpC cefoxitin-EDTA test showed a higher discriminatory power for detecting AmpC β-lactamase-producing strains. The specificity and sensitivity of AmpC cefoxitin-EDTA were 77%, 100% for
K. pneumonia
and 70%, 90% for
E. coli
higher than the other two tests, respectively. Also, the authors demonstrated high prevalence rate for resistance to certain antibiotics, such as cefuroxime, trimethoprim-sulfamethoxazole, ampicillin, and cefotaxime. In conclusion, our study provided valuable information regarding the plasmid-mediated
AmpC
β-lactamase gene content, antibiotic resistance, and confirmatory phenotypic tests for AmpC β-lactamases in
E. coli
and
K. pneumoniae
isolates from clinical sources. |
| doi_str_mv | 10.1007/s00284-021-02479-9 |
| format | Article |
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Klebsiella pneumoniae
and
Escherichia coli
resistance to β-lactam antibiotics is the production of β-lactamase enzymes. Among these are the AmpC β-lactamases, which confer resistance to a class of antibiotics. However, little is known about the AmpC β-lactamases of
K. pneumoniae
and
E. coli
clinical isolates in Qazvin, Iran. This study was designed to assess the AmpC β‑lactamases-producing strains and also identify the prevalence of
AmpC
β‑lactamases genes. Antimicrobial susceptibility tests were performed on 435
K. pneumoniae
and
E. coli
isolates using disk diffusion technique. Plasmid-mediated
AmpC
genes were studied using a multiplex PCR assay. The AmpC β-lactamase-producer isolates were studied by employing cefoxitin disk diffusion test, AmpC induction test, AmpC cefoxitin-EDTA test, and boronic acid disk test. Our results showed that of 46 (18.4%) cefoxitin-insensitive
E. coli
isolates, 10 (21.7%) were positive for
AmpC
β-lactamase genes, among them 4 (8.69%) isolates were positive for
bla
DHA
genes and 6 (13%) for
bla
CIT
genes. Of 57 (30.4%) cefoxitin-insensitive
K. pneumoniae
isolates, 10 (17.5%) were positive for AmpC gene with 4 (6.34%) and 6 (9.5%) isolates positive for
bla
DHA
and
bla
CIT
genes, respectively. However, no
MOX
,
ACC
,
FOX
, or
EBC
genes were detected in the isolates. Considering the results of different confirmatory phenotypic tests, the AmpC cefoxitin-EDTA test showed a higher discriminatory power for detecting AmpC β-lactamase-producing strains. The specificity and sensitivity of AmpC cefoxitin-EDTA were 77%, 100% for
K. pneumonia
and 70%, 90% for
E. coli
higher than the other two tests, respectively. Also, the authors demonstrated high prevalence rate for resistance to certain antibiotics, such as cefuroxime, trimethoprim-sulfamethoxazole, ampicillin, and cefotaxime. In conclusion, our study provided valuable information regarding the plasmid-mediated
AmpC
β-lactamase gene content, antibiotic resistance, and confirmatory phenotypic tests for AmpC β-lactamases in
E. coli
and
K. pneumoniae
isolates from clinical sources.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-021-02479-9</identifier><identifier>PMID: 33837818</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Amides ; AmpC gene ; Ampicillin ; Antibiotic resistance ; Antibiotics ; Antiinfectives and antibacterials ; Biomedical and Life Sciences ; Biotechnology ; Cefotaxime ; Cefoxitin ; Cefuroxime ; Clinical isolates ; E coli ; Edetic acid ; Escherichia coli ; Ethylenediaminetetraacetic acids ; Genes ; Klebsiella ; Klebsiella pneumoniae ; Life Sciences ; Life Sciences & Biomedicine ; Microbiology ; Pneumonia ; Science & Technology ; Short Communication ; Strains (organisms) ; Sulfamethoxazole ; Trimethoprim ; Trimethoprim-sulfamethoxazole ; β Lactamase ; β-Lactam antibiotics</subject><ispartof>Current microbiology, 2021-06, Vol.78 (6), p.2317-2323</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>14</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000638820700002</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c419t-52e157d8d6dcb9c2b059e26d98dbd343494e3d84c2d8f3efbb6186b9e3c7dfd93</citedby><cites>FETCH-LOGICAL-c419t-52e157d8d6dcb9c2b059e26d98dbd343494e3d84c2d8f3efbb6186b9e3c7dfd93</cites><orcidid>0000-0003-0369-8179</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-021-02479-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-021-02479-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,39263,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33837818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robatjazi, Saeedeh</creatorcontrib><creatorcontrib>Nikkhahi, Farhad</creatorcontrib><creatorcontrib>Niazadeh, Mojtaba</creatorcontrib><creatorcontrib>Amin Marashi, Seyed Mahmoud</creatorcontrib><creatorcontrib>Peymani, Amir</creatorcontrib><creatorcontrib>Javadi, Amir</creatorcontrib><creatorcontrib>Kashani, Amir Hossein</creatorcontrib><title>Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>CURR MICROBIOL</addtitle><addtitle>Curr Microbiol</addtitle><description>One of the mechanisms of
Klebsiella pneumoniae
and
Escherichia coli
resistance to β-lactam antibiotics is the production of β-lactamase enzymes. Among these are the AmpC β-lactamases, which confer resistance to a class of antibiotics. However, little is known about the AmpC β-lactamases of
K. pneumoniae
and
E. coli
clinical isolates in Qazvin, Iran. This study was designed to assess the AmpC β‑lactamases-producing strains and also identify the prevalence of
AmpC
β‑lactamases genes. Antimicrobial susceptibility tests were performed on 435
K. pneumoniae
and
E. coli
isolates using disk diffusion technique. Plasmid-mediated
AmpC
genes were studied using a multiplex PCR assay. The AmpC β-lactamase-producer isolates were studied by employing cefoxitin disk diffusion test, AmpC induction test, AmpC cefoxitin-EDTA test, and boronic acid disk test. Our results showed that of 46 (18.4%) cefoxitin-insensitive
E. coli
isolates, 10 (21.7%) were positive for
AmpC
β-lactamase genes, among them 4 (8.69%) isolates were positive for
bla
DHA
genes and 6 (13%) for
bla
CIT
genes. Of 57 (30.4%) cefoxitin-insensitive
K. pneumoniae
isolates, 10 (17.5%) were positive for AmpC gene with 4 (6.34%) and 6 (9.5%) isolates positive for
bla
DHA
and
bla
CIT
genes, respectively. However, no
MOX
,
ACC
,
FOX
, or
EBC
genes were detected in the isolates. Considering the results of different confirmatory phenotypic tests, the AmpC cefoxitin-EDTA test showed a higher discriminatory power for detecting AmpC β-lactamase-producing strains. The specificity and sensitivity of AmpC cefoxitin-EDTA were 77%, 100% for
K. pneumonia
and 70%, 90% for
E. coli
higher than the other two tests, respectively. Also, the authors demonstrated high prevalence rate for resistance to certain antibiotics, such as cefuroxime, trimethoprim-sulfamethoxazole, ampicillin, and cefotaxime. In conclusion, our study provided valuable information regarding the plasmid-mediated
AmpC
β-lactamase gene content, antibiotic resistance, and confirmatory phenotypic tests for AmpC β-lactamases in
E. coli
and
K. pneumoniae
isolates from clinical sources.</description><subject>Amides</subject><subject>AmpC gene</subject><subject>Ampicillin</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cefotaxime</subject><subject>Cefoxitin</subject><subject>Cefuroxime</subject><subject>Clinical isolates</subject><subject>E coli</subject><subject>Edetic acid</subject><subject>Escherichia coli</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>Genes</subject><subject>Klebsiella</subject><subject>Klebsiella pneumoniae</subject><subject>Life Sciences</subject><subject>Life Sciences & Biomedicine</subject><subject>Microbiology</subject><subject>Pneumonia</subject><subject>Science & Technology</subject><subject>Short Communication</subject><subject>Strains (organisms)</subject><subject>Sulfamethoxazole</subject><subject>Trimethoprim</subject><subject>Trimethoprim-sulfamethoxazole</subject><subject>β Lactamase</subject><subject>β-Lactam 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Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran</title><author>Robatjazi, Saeedeh ; Nikkhahi, Farhad ; Niazadeh, Mojtaba ; Amin Marashi, Seyed Mahmoud ; Peymani, Amir ; Javadi, Amir ; Kashani, Amir Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-52e157d8d6dcb9c2b059e26d98dbd343494e3d84c2d8f3efbb6186b9e3c7dfd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amides</topic><topic>AmpC gene</topic><topic>Ampicillin</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cefotaxime</topic><topic>Cefoxitin</topic><topic>Cefuroxime</topic><topic>Clinical isolates</topic><topic>E coli</topic><topic>Edetic acid</topic><topic>Escherichia coli</topic><topic>Ethylenediaminetetraacetic acids</topic><topic>Genes</topic><topic>Klebsiella</topic><topic>Klebsiella pneumoniae</topic><topic>Life Sciences</topic><topic>Life Sciences & Biomedicine</topic><topic>Microbiology</topic><topic>Pneumonia</topic><topic>Science & Technology</topic><topic>Short Communication</topic><topic>Strains (organisms)</topic><topic>Sulfamethoxazole</topic><topic>Trimethoprim</topic><topic>Trimethoprim-sulfamethoxazole</topic><topic>β Lactamase</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robatjazi, Saeedeh</creatorcontrib><creatorcontrib>Nikkhahi, Farhad</creatorcontrib><creatorcontrib>Niazadeh, Mojtaba</creatorcontrib><creatorcontrib>Amin Marashi, Seyed Mahmoud</creatorcontrib><creatorcontrib>Peymani, Amir</creatorcontrib><creatorcontrib>Javadi, Amir</creatorcontrib><creatorcontrib>Kashani, Amir Hossein</creatorcontrib><collection>Web 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Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robatjazi, Saeedeh</au><au>Nikkhahi, Farhad</au><au>Niazadeh, Mojtaba</au><au>Amin Marashi, Seyed Mahmoud</au><au>Peymani, Amir</au><au>Javadi, Amir</au><au>Kashani, Amir Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><stitle>CURR MICROBIOL</stitle><addtitle>Curr Microbiol</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>78</volume><issue>6</issue><spage>2317</spage><epage>2323</epage><pages>2317-2323</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>One of the mechanisms of
Klebsiella pneumoniae
and
Escherichia coli
resistance to β-lactam antibiotics is the production of β-lactamase enzymes. Among these are the AmpC β-lactamases, which confer resistance to a class of antibiotics. However, little is known about the AmpC β-lactamases of
K. pneumoniae
and
E. coli
clinical isolates in Qazvin, Iran. This study was designed to assess the AmpC β‑lactamases-producing strains and also identify the prevalence of
AmpC
β‑lactamases genes. Antimicrobial susceptibility tests were performed on 435
K. pneumoniae
and
E. coli
isolates using disk diffusion technique. Plasmid-mediated
AmpC
genes were studied using a multiplex PCR assay. The AmpC β-lactamase-producer isolates were studied by employing cefoxitin disk diffusion test, AmpC induction test, AmpC cefoxitin-EDTA test, and boronic acid disk test. Our results showed that of 46 (18.4%) cefoxitin-insensitive
E. coli
isolates, 10 (21.7%) were positive for
AmpC
β-lactamase genes, among them 4 (8.69%) isolates were positive for
bla
DHA
genes and 6 (13%) for
bla
CIT
genes. Of 57 (30.4%) cefoxitin-insensitive
K. pneumoniae
isolates, 10 (17.5%) were positive for AmpC gene with 4 (6.34%) and 6 (9.5%) isolates positive for
bla
DHA
and
bla
CIT
genes, respectively. However, no
MOX
,
ACC
,
FOX
, or
EBC
genes were detected in the isolates. Considering the results of different confirmatory phenotypic tests, the AmpC cefoxitin-EDTA test showed a higher discriminatory power for detecting AmpC β-lactamase-producing strains. The specificity and sensitivity of AmpC cefoxitin-EDTA were 77%, 100% for
K. pneumonia
and 70%, 90% for
E. coli
higher than the other two tests, respectively. Also, the authors demonstrated high prevalence rate for resistance to certain antibiotics, such as cefuroxime, trimethoprim-sulfamethoxazole, ampicillin, and cefotaxime. In conclusion, our study provided valuable information regarding the plasmid-mediated
AmpC
β-lactamase gene content, antibiotic resistance, and confirmatory phenotypic tests for AmpC β-lactamases in
E. coli
and
K. pneumoniae
isolates from clinical sources.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33837818</pmid><doi>10.1007/s00284-021-02479-9</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0369-8179</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 2021-06, Vol.78 (6), p.2317-2323 |
| issn | 0343-8651 1432-0991 |
| language | eng |
| recordid | cdi_proquest_journals_2533058371 |
| source | SpringerNature Journals; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /> |
| subjects | Amides AmpC gene Ampicillin Antibiotic resistance Antibiotics Antiinfectives and antibacterials Biomedical and Life Sciences Biotechnology Cefotaxime Cefoxitin Cefuroxime Clinical isolates E coli Edetic acid Escherichia coli Ethylenediaminetetraacetic acids Genes Klebsiella Klebsiella pneumoniae Life Sciences Life Sciences & Biomedicine Microbiology Pneumonia Science & Technology Short Communication Strains (organisms) Sulfamethoxazole Trimethoprim Trimethoprim-sulfamethoxazole β Lactamase β-Lactam antibiotics |
| title | Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T21%3A11%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phenotypic%20Identification%20and%20Genotypic%20Characterization%20of%20Plasmid-Mediated%20AmpC%20%CE%B2-Lactamase-Producing%20Escherichia%20coli%20and%20Klebsiella%20pneumoniae%20Isolates%20in%20Iran&rft.jtitle=Current%20microbiology&rft.au=Robatjazi,%20Saeedeh&rft.date=2021-06-01&rft.volume=78&rft.issue=6&rft.spage=2317&rft.epage=2323&rft.pages=2317-2323&rft.issn=0343-8651&rft.eissn=1432-0991&rft_id=info:doi/10.1007/s00284-021-02479-9&rft_dat=%3Cproquest_pubme%3E2511237873%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2533058371&rft_id=info:pmid/33837818&rfr_iscdi=true |