Immunostimulatory activities of a high molecular weight fraction of Cynanchum auriculatum royle ex wight root obtained by ultrafiltration
Background: The root of Cynanchum auriculatum Royle ex Wight has been traditionally used as a folk medicine in the eastern Asia. Objectives: In this study, we explored the immune-enhancing effect of the high-molecular weight fraction of C. auriculatum (CAHF) root extracts in RAW 264.7 macrophages an...
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| Veröffentlicht in: | Pharmacognosy Magazine 2020-07, Vol.16 (71), p.493-498 |
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| creator | Jang, Mi Lim, Tae-Gyu Lee, Eunjung Hong, Hee-Do Rhee, Young Lee, Yun Lee, Jeong Cho, Chang-Won |
| description | Background: The root of Cynanchum auriculatum Royle ex Wight has been traditionally used as a folk medicine in the eastern Asia. Objectives: In this study, we explored the immune-enhancing effect of the high-molecular weight fraction of C. auriculatum (CAHF) root extracts in RAW 264.7 macrophages and cyclophosphamide (CYP)-induced immune-suppressed mice.
Materials and Methods: To obtain the CAHF, ultrafiltration was performed using 30-kDa molecular weight cutoff polyethersulfone membranes in a cross-flow microfiltration system. In vitro and in vivo experiments were performed to evaluate the immunostimulatory effects of CAHF. Results: CAHF increased the production of nitric oxide and immunostimulatory cytokines (interleukin-6 and tumor necrosis factor-α) in recombinant interferon-γ primed macrophages. In addition, the phagocytic activity of macrophage cells was enhanced by CAHF. Furthermore, CAHF treatment markedly restored the decreased body weight, immune organs (spleen and thymus) weight, natural killer cell activity, and T- and B-lymphocyte proliferation in CYP-induced immune-suppressed mice. Conclusion: These results suggest that CAHF is an effective immunostimulatory agent. |
| doi_str_mv | 10.4103/pm.pm_449_19 |
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Materials and Methods: To obtain the CAHF, ultrafiltration was performed using 30-kDa molecular weight cutoff polyethersulfone membranes in a cross-flow microfiltration system. In vitro and in vivo experiments were performed to evaluate the immunostimulatory effects of CAHF. Results: CAHF increased the production of nitric oxide and immunostimulatory cytokines (interleukin-6 and tumor necrosis factor-α) in recombinant interferon-γ primed macrophages. In addition, the phagocytic activity of macrophage cells was enhanced by CAHF. Furthermore, CAHF treatment markedly restored the decreased body weight, immune organs (spleen and thymus) weight, natural killer cell activity, and T- and B-lymphocyte proliferation in CYP-induced immune-suppressed mice. Conclusion: These results suggest that CAHF is an effective immunostimulatory agent.</description><identifier>ISSN: 0973-1296</identifier><identifier>EISSN: 0976-4062</identifier><identifier>DOI: 10.4103/pm.pm_449_19</identifier><language>eng</language><publisher>London: Wolters Kluwer India Pvt. Ltd</publisher><subject>Cyclophosphamide ; Cytochrome P-450 ; Macrophages ; Molecular weight</subject><ispartof>Pharmacognosy Magazine, 2020-07, Vol.16 (71), p.493-498</ispartof><rights>COPYRIGHT 2020 Medknow Publications and Media Pvt. Ltd.</rights><rights>2020. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-b6710f457efd096f43663c111e4521773c4ebdc7b5b08df4d8086281dec95ade3</citedby><cites>FETCH-LOGICAL-c315t-b6710f457efd096f43663c111e4521773c4ebdc7b5b08df4d8086281dec95ade3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Jang, Mi</creatorcontrib><creatorcontrib>Lim, Tae-Gyu</creatorcontrib><creatorcontrib>Lee, Eunjung</creatorcontrib><creatorcontrib>Hong, Hee-Do</creatorcontrib><creatorcontrib>Rhee, Young</creatorcontrib><creatorcontrib>Lee, Yun</creatorcontrib><creatorcontrib>Lee, Jeong</creatorcontrib><creatorcontrib>Cho, Chang-Won</creatorcontrib><title>Immunostimulatory activities of a high molecular weight fraction of Cynanchum auriculatum royle ex wight root obtained by ultrafiltration</title><title>Pharmacognosy Magazine</title><description>Background: The root of Cynanchum auriculatum Royle ex Wight has been traditionally used as a folk medicine in the eastern Asia. Objectives: In this study, we explored the immune-enhancing effect of the high-molecular weight fraction of C. auriculatum (CAHF) root extracts in RAW 264.7 macrophages and cyclophosphamide (CYP)-induced immune-suppressed mice.
Materials and Methods: To obtain the CAHF, ultrafiltration was performed using 30-kDa molecular weight cutoff polyethersulfone membranes in a cross-flow microfiltration system. In vitro and in vivo experiments were performed to evaluate the immunostimulatory effects of CAHF. Results: CAHF increased the production of nitric oxide and immunostimulatory cytokines (interleukin-6 and tumor necrosis factor-α) in recombinant interferon-γ primed macrophages. In addition, the phagocytic activity of macrophage cells was enhanced by CAHF. Furthermore, CAHF treatment markedly restored the decreased body weight, immune organs (spleen and thymus) weight, natural killer cell activity, and T- and B-lymphocyte proliferation in CYP-induced immune-suppressed mice. Conclusion: These results suggest that CAHF is an effective immunostimulatory agent.</description><subject>Cyclophosphamide</subject><subject>Cytochrome P-450</subject><subject>Macrophages</subject><subject>Molecular weight</subject><issn>0973-1296</issn><issn>0976-4062</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkdFqFTEQhhdRsFbvfICAt-5psslmNzdCOagtFLzR65DNTnrSbpJjknW7j-Bbm-3RglAGMjPw_ZNM_qp6T_COEUwvjm53dJIxIYl4UZ1h0fGaYd68fKxpTRrBX1dvUrrDuO0J7s6q39fOzT6kbN08qRziipTO9pfNFhIKBil0sLcH5MIEuhARLVD6jEzcuOA3Zr965fVhdkjN0W5YLnUM6wQIHtDyKIghZBSGrKyHEQ0rmqcclbHbuQ16W70yakrw7m8-r358-fx9f1XffPt6vb-8qTUlba4H3hFsWNuBGbHghlHOqSaEAGsb0nVUMxhG3Q3tgPvRsLHHPW96MoIWrRqBnlcfTnOPMfycIWV5F-boy5WyaWkjGtyXv3qibtUE0noTyjO1s0nLS05F3_dU4ELtnqFKjOCsDh7KevC_4ONJoGNIKYKRx2idiqskWG4eymLgk4cF_3TClzBliOl-mheI0sF478PyrEYyQeU_U-kfvzuosA</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Jang, Mi</creator><creator>Lim, Tae-Gyu</creator><creator>Lee, Eunjung</creator><creator>Hong, Hee-Do</creator><creator>Rhee, Young</creator><creator>Lee, Yun</creator><creator>Lee, Jeong</creator><creator>Cho, Chang-Won</creator><general>Wolters Kluwer India Pvt. 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Objectives: In this study, we explored the immune-enhancing effect of the high-molecular weight fraction of C. auriculatum (CAHF) root extracts in RAW 264.7 macrophages and cyclophosphamide (CYP)-induced immune-suppressed mice.
Materials and Methods: To obtain the CAHF, ultrafiltration was performed using 30-kDa molecular weight cutoff polyethersulfone membranes in a cross-flow microfiltration system. In vitro and in vivo experiments were performed to evaluate the immunostimulatory effects of CAHF. Results: CAHF increased the production of nitric oxide and immunostimulatory cytokines (interleukin-6 and tumor necrosis factor-α) in recombinant interferon-γ primed macrophages. In addition, the phagocytic activity of macrophage cells was enhanced by CAHF. Furthermore, CAHF treatment markedly restored the decreased body weight, immune organs (spleen and thymus) weight, natural killer cell activity, and T- and B-lymphocyte proliferation in CYP-induced immune-suppressed mice. Conclusion: These results suggest that CAHF is an effective immunostimulatory agent.</abstract><cop>London</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><doi>10.4103/pm.pm_449_19</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cyclophosphamide Cytochrome P-450 Macrophages Molecular weight |
| title | Immunostimulatory activities of a high molecular weight fraction of Cynanchum auriculatum royle ex wight root obtained by ultrafiltration |
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