The regulatory role of root in cadmium accumulation in a high cadmium-accumulating rice line (Oryza sativa L.)

There are some key processes that regulate cadmium (Cd) accumulation in rice. Understanding the characteristics and mechanisms of Cd accumulation in high Cd-accumulating rice lines benefits for excavating relevant genes. Cd accumulation and distribution in roots of Lu527-8, a high Cd-accumulating rice line, were investigated by a hydroponic experiment, with a control of a normal rice line (Lu527-4). Lu527-8 showed significantly higher Cd concentrations in roots than Lu527-4. More than 81% of Cd in roots of two rice lines is distributed in soluble fraction and cell wall. In soluble fraction, there were more organic acids, amino acids, and phytochelatins in Lu527-8, benefiting Cd accumulation. Pectin and hemicellulose 1 (HC1), especially pectin, were main polysaccharides in cell wall. Lu527-8 showed more pectin and HC1 along with higher pectin methylesterase (PME) activity compared with Lu527-4, promoting Cd accumulation. Besides, Lu527-8 showed higher Cd translocation from root to shoot due to more amounts of ethanol-extractable Cd in roots than Lu527-4. In conclusion, specific characteristics of Cd chemical forms and subcellular distribution in roots of high Cd-accumulating rice line are important for Cd accumulation and translocation.