Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin-agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles
Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr 2 O 4 ) nanoparticles. Considering the cell v...
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| Veröffentlicht in: | RSC advances 2021-05, Vol.11 (29), p.17914-17923 |
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| creator | Eivazzadeh-Keihan, Reza Moghim Aliabadi, Hooman Aghamirza Radinekiyan, Fateme Sobhani, Mohammad Farzane khalili Maleki, Ali Madanchi, Hamid Mahdavi, Mohammad Shalan, Ahmed Esmail |
| description | Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles. Considering the cell viability technique, red blood cell hemolysis in addition to anti-biofilm assays, it was determined that after three days, the toxicity of the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite was less than 13%. Moreover, the small hemolytic effect (1.67%) and high level of prevention in forming a
P. aeruginosa
biofilm with low OD value (0.18) showed signs of considerable hemocompatibility and antibacterial activity. Besides, according to an
in vivo
assay study, the wounds of mice treated with the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite scaffold were almost completely healed in five days. Aside from these biological tests, the structural features were evaluated by FT-IR, EDX, FE-SEM, and TG analyses, as well as swelling ratio, rheological, and compressive mechanical study tests. Additionally, it was concluded that adding silk fibroin and ZnCr
2
O
4
nanoparticles could enhance the mechanical tensile properties of cross-linked lignin-agarose hydrogel, and also an elastic network was characterized for this designed nanobiocomposite.
Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles. |
| doi_str_mv | 10.1039/d1ra01300a |
| format | Article |
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2
O
4
) nanoparticles. Considering the cell viability technique, red blood cell hemolysis in addition to anti-biofilm assays, it was determined that after three days, the toxicity of the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite was less than 13%. Moreover, the small hemolytic effect (1.67%) and high level of prevention in forming a
P. aeruginosa
biofilm with low OD value (0.18) showed signs of considerable hemocompatibility and antibacterial activity. Besides, according to an
in vivo
assay study, the wounds of mice treated with the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite scaffold were almost completely healed in five days. Aside from these biological tests, the structural features were evaluated by FT-IR, EDX, FE-SEM, and TG analyses, as well as swelling ratio, rheological, and compressive mechanical study tests. Additionally, it was concluded that adding silk fibroin and ZnCr
2
O
4
nanoparticles could enhance the mechanical tensile properties of cross-linked lignin-agarose hydrogel, and also an elastic network was characterized for this designed nanobiocomposite.
Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d1ra01300a</identifier><identifier>PMID: 35480185</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Biocompatibility ; Biofilms ; Biological activity ; Biological properties ; Chemistry ; Chromite ; Crosslinking ; Erythrocytes ; Hydrogels ; In vivo methods and tests ; Infrared spectroscopy ; Lignin ; Mechanical properties ; Nanoparticles ; Rheological properties ; Scaffolds ; Silk fibroin ; Swelling ratio ; Tensile properties ; Toxicity ; Wound healing ; Zinc</subject><ispartof>RSC advances, 2021-05, Vol.11 (29), p.17914-17923</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2021</rights><rights>This journal is © The Royal Society of Chemistry 2021 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-c93f30415295c95b605d0f282106021109ac550a18b11970bdf6701ab503c8183</citedby><cites>FETCH-LOGICAL-c428t-c93f30415295c95b605d0f282106021109ac550a18b11970bdf6701ab503c8183</cites><orcidid>0000-0002-3424-1609 ; 0000-0001-5490-3350 ; 0000-0003-2150-5721 ; 0000-0002-8007-2543</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033182/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033182/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35480185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eivazzadeh-Keihan, Reza</creatorcontrib><creatorcontrib>Moghim Aliabadi, Hooman Aghamirza</creatorcontrib><creatorcontrib>Radinekiyan, Fateme</creatorcontrib><creatorcontrib>Sobhani, Mohammad</creatorcontrib><creatorcontrib>Farzane khalili</creatorcontrib><creatorcontrib>Maleki, Ali</creatorcontrib><creatorcontrib>Madanchi, Hamid</creatorcontrib><creatorcontrib>Mahdavi, Mohammad</creatorcontrib><creatorcontrib>Shalan, Ahmed Esmail</creatorcontrib><title>Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin-agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles. Considering the cell viability technique, red blood cell hemolysis in addition to anti-biofilm assays, it was determined that after three days, the toxicity of the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite was less than 13%. Moreover, the small hemolytic effect (1.67%) and high level of prevention in forming a
P. aeruginosa
biofilm with low OD value (0.18) showed signs of considerable hemocompatibility and antibacterial activity. Besides, according to an
in vivo
assay study, the wounds of mice treated with the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite scaffold were almost completely healed in five days. Aside from these biological tests, the structural features were evaluated by FT-IR, EDX, FE-SEM, and TG analyses, as well as swelling ratio, rheological, and compressive mechanical study tests. Additionally, it was concluded that adding silk fibroin and ZnCr
2
O
4
nanoparticles could enhance the mechanical tensile properties of cross-linked lignin-agarose hydrogel, and also an elastic network was characterized for this designed nanobiocomposite.
Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles.</description><subject>Biocompatibility</subject><subject>Biofilms</subject><subject>Biological activity</subject><subject>Biological properties</subject><subject>Chemistry</subject><subject>Chromite</subject><subject>Crosslinking</subject><subject>Erythrocytes</subject><subject>Hydrogels</subject><subject>In vivo methods and tests</subject><subject>Infrared spectroscopy</subject><subject>Lignin</subject><subject>Mechanical properties</subject><subject>Nanoparticles</subject><subject>Rheological properties</subject><subject>Scaffolds</subject><subject>Silk fibroin</subject><subject>Swelling ratio</subject><subject>Tensile properties</subject><subject>Toxicity</subject><subject>Wound healing</subject><subject>Zinc</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkk9v1DAQxSMEolXphTvIEhdUERg7sTe-VFqVf5UqISE4RxPHSVwcO9jJouUT9mPh7bZLwQfb8vz89Gb0suw5hbcUCvmupQGBFgD4KDtmUIqcgZCPH9yPstMYryEtwSkT9Gl2VPCyAlrx4-zm0m10nE2Ps_GO-I7MgyaN8db3RqElqGazMfP2DRm1GtDdPk7BTzrMRkeCriW__JL2QaM1ric4TTZR93pInN9oS6LCrvO2JQ1G3ZJUtKZ3xuXYY_BRk2HbBt8ncicZjf1BOtMEbxzRY6PbNn36bZwiagh-NLMmDp2fMNlQVsdn2ZMObdSnd-dJ9v3jh28Xn_OrL58uL9ZXuSpZNedKFl0BJeVMciV5I4C30LGKURDAKAWJinNAWjWUyhU0bSdWQLHhUKiKVsVJdr7XnZZm1K3Sbg5o6ymYEcO29mjqfyvODHXvN7WEoqAVSwKv7wSC_7mk2dejiUpbi077JdZMcLEqqSwhoa_-Q6_9Elxqr2apAZBCljtHZ3tKpTHGoLuDGQr1LiP1e_p1fZuRdYJfPrR_QO8TkYAXeyBEdaj-DVnxB8HNxNo</recordid><startdate>20210518</startdate><enddate>20210518</enddate><creator>Eivazzadeh-Keihan, Reza</creator><creator>Moghim Aliabadi, Hooman Aghamirza</creator><creator>Radinekiyan, Fateme</creator><creator>Sobhani, Mohammad</creator><creator>Farzane khalili</creator><creator>Maleki, Ali</creator><creator>Madanchi, Hamid</creator><creator>Mahdavi, Mohammad</creator><creator>Shalan, Ahmed Esmail</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3424-1609</orcidid><orcidid>https://orcid.org/0000-0001-5490-3350</orcidid><orcidid>https://orcid.org/0000-0003-2150-5721</orcidid><orcidid>https://orcid.org/0000-0002-8007-2543</orcidid></search><sort><creationdate>20210518</creationdate><title>Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin-agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles</title><author>Eivazzadeh-Keihan, Reza ; Moghim Aliabadi, Hooman Aghamirza ; Radinekiyan, Fateme ; Sobhani, Mohammad ; Farzane khalili ; Maleki, Ali ; Madanchi, Hamid ; Mahdavi, Mohammad ; Shalan, Ahmed Esmail</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-c93f30415295c95b605d0f282106021109ac550a18b11970bdf6701ab503c8183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biocompatibility</topic><topic>Biofilms</topic><topic>Biological activity</topic><topic>Biological properties</topic><topic>Chemistry</topic><topic>Chromite</topic><topic>Crosslinking</topic><topic>Erythrocytes</topic><topic>Hydrogels</topic><topic>In vivo methods and tests</topic><topic>Infrared spectroscopy</topic><topic>Lignin</topic><topic>Mechanical properties</topic><topic>Nanoparticles</topic><topic>Rheological properties</topic><topic>Scaffolds</topic><topic>Silk fibroin</topic><topic>Swelling ratio</topic><topic>Tensile properties</topic><topic>Toxicity</topic><topic>Wound healing</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eivazzadeh-Keihan, Reza</creatorcontrib><creatorcontrib>Moghim Aliabadi, Hooman Aghamirza</creatorcontrib><creatorcontrib>Radinekiyan, Fateme</creatorcontrib><creatorcontrib>Sobhani, Mohammad</creatorcontrib><creatorcontrib>Farzane khalili</creatorcontrib><creatorcontrib>Maleki, Ali</creatorcontrib><creatorcontrib>Madanchi, Hamid</creatorcontrib><creatorcontrib>Mahdavi, Mohammad</creatorcontrib><creatorcontrib>Shalan, Ahmed Esmail</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eivazzadeh-Keihan, Reza</au><au>Moghim Aliabadi, Hooman Aghamirza</au><au>Radinekiyan, Fateme</au><au>Sobhani, Mohammad</au><au>Farzane khalili</au><au>Maleki, Ali</au><au>Madanchi, Hamid</au><au>Mahdavi, Mohammad</au><au>Shalan, Ahmed Esmail</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin-agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2021-05-18</date><risdate>2021</risdate><volume>11</volume><issue>29</issue><spage>17914</spage><epage>17923</epage><pages>17914-17923</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles. Considering the cell viability technique, red blood cell hemolysis in addition to anti-biofilm assays, it was determined that after three days, the toxicity of the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite was less than 13%. Moreover, the small hemolytic effect (1.67%) and high level of prevention in forming a
P. aeruginosa
biofilm with low OD value (0.18) showed signs of considerable hemocompatibility and antibacterial activity. Besides, according to an
in vivo
assay study, the wounds of mice treated with the cross-linked lignin-agarose/SF/ZnCr
2
O
4
nanobiocomposite scaffold were almost completely healed in five days. Aside from these biological tests, the structural features were evaluated by FT-IR, EDX, FE-SEM, and TG analyses, as well as swelling ratio, rheological, and compressive mechanical study tests. Additionally, it was concluded that adding silk fibroin and ZnCr
2
O
4
nanoparticles could enhance the mechanical tensile properties of cross-linked lignin-agarose hydrogel, and also an elastic network was characterized for this designed nanobiocomposite.
Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin-agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr
2
O
4
) nanoparticles.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35480185</pmid><doi>10.1039/d1ra01300a</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3424-1609</orcidid><orcidid>https://orcid.org/0000-0001-5490-3350</orcidid><orcidid>https://orcid.org/0000-0003-2150-5721</orcidid><orcidid>https://orcid.org/0000-0002-8007-2543</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | RSC advances, 2021-05, Vol.11 (29), p.17914-17923 |
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| language | eng |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Biocompatibility Biofilms Biological activity Biological properties Chemistry Chromite Crosslinking Erythrocytes Hydrogels In vivo methods and tests Infrared spectroscopy Lignin Mechanical properties Nanoparticles Rheological properties Scaffolds Silk fibroin Swelling ratio Tensile properties Toxicity Wound healing Zinc |
| title | Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin-agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles |
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