In Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam
This study aimed to observe the effect of toxicity of the pesticide thiamethoxam (TMX) at sublethal concentrations in the liver and brain of Oreochromis niloticus . In the experiment, fish were exposed to 50, 100, and, 150 mg/L with thiamethoxam for 48 h and 15 days. The superoxide dismutase (SOD),...
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| description | This study aimed to observe the effect of toxicity of the pesticide thiamethoxam (TMX) at sublethal concentrations in the liver and brain of
Oreochromis niloticus
. In the experiment, fish were exposed to 50, 100, and, 150 mg/L with thiamethoxam for 48 h and 15 days. The superoxide dismutase (SOD), catalase (CAT), glutathione S-transferaz (GST), glutathione peroxidase (GPx), and ethoxyresorufin-
O
-deethylase (EROD) activities; and thiobarbituric acid reactive substance (TBARS), heat shock proteins 70 (HSP70), glutathione (GSH), and genotoxicity parameter 8-hydroxy-2′-deoxyguanosine (8-OHdG) were analyzed by spectrophotometric methods and ELISA techniques. Depending on time and dose in TMX exposure in liver tissue, a significant decrease in GSH level; an increase in SOD, GST, GPx, and EROD enzyme activities; and HSP70, TBARS, and 8-OHdG levels was determined. In brain tissue, SOD, GST, and EROD enzyme activities, an increase in HSP70, TBARS, and 8-OHdG levels, and a decrease in CAT enzyme activity and GSH levels were determined. In this study, TMX in the concentrations used showed that changes in oxidative stress biomarkers, genotoxicity parameter 8-OHdG levels, and HSP70 levels caused toxic effects in the model organism. As a result of the study, the changes and protective effects of the antioxidant system and stress proteins at the cellular level were determined in sublethal doses of toxic effects caused by TMX in the vital organs of the organism. In this toxicological study, TMX exposure resulted in toxicity to
O. niloticus
liver and brain tissues, in addition, responses of biomarkers to time and concentrations were determined. As a result of this study, the potential toxic effects of the commonly used pesticide TMX will reveal both the ecological risks of the aquatic organism and the basic data of the safety and risk assessments of
O. niloticus
consumed as food for human health. |
| doi_str_mv | 10.1007/s11270-021-05101-7 |
| format | Article |
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Oreochromis niloticus
. In the experiment, fish were exposed to 50, 100, and, 150 mg/L with thiamethoxam for 48 h and 15 days. The superoxide dismutase (SOD), catalase (CAT), glutathione S-transferaz (GST), glutathione peroxidase (GPx), and ethoxyresorufin-
O
-deethylase (EROD) activities; and thiobarbituric acid reactive substance (TBARS), heat shock proteins 70 (HSP70), glutathione (GSH), and genotoxicity parameter 8-hydroxy-2′-deoxyguanosine (8-OHdG) were analyzed by spectrophotometric methods and ELISA techniques. Depending on time and dose in TMX exposure in liver tissue, a significant decrease in GSH level; an increase in SOD, GST, GPx, and EROD enzyme activities; and HSP70, TBARS, and 8-OHdG levels was determined. In brain tissue, SOD, GST, and EROD enzyme activities, an increase in HSP70, TBARS, and 8-OHdG levels, and a decrease in CAT enzyme activity and GSH levels were determined. In this study, TMX in the concentrations used showed that changes in oxidative stress biomarkers, genotoxicity parameter 8-OHdG levels, and HSP70 levels caused toxic effects in the model organism. As a result of the study, the changes and protective effects of the antioxidant system and stress proteins at the cellular level were determined in sublethal doses of toxic effects caused by TMX in the vital organs of the organism. In this toxicological study, TMX exposure resulted in toxicity to
O. niloticus
liver and brain tissues, in addition, responses of biomarkers to time and concentrations were determined. As a result of this study, the potential toxic effects of the commonly used pesticide TMX will reveal both the ecological risks of the aquatic organism and the basic data of the safety and risk assessments of
O. niloticus
consumed as food for human health.</description><identifier>ISSN: 0049-6979</identifier><identifier>EISSN: 1573-2932</identifier><identifier>DOI: 10.1007/s11270-021-05101-7</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>8-Hydroxydeoxyguanosine ; Analysis ; Antioxidants ; Aquatic organisms ; Atmospheric Protection/Air Quality Control/Air Pollution ; Biocompatibility ; Biological stress ; Biomarkers ; Body organs ; Brain ; Catalase ; Chloramphenicol O-acetyltransferase ; Climate Change/Climate Change Impacts ; Deoxyguanosine ; Earth and Environmental Science ; ELISA ; Environment ; Environmental monitoring ; Enzymatic activity ; Enzyme activity ; Enzyme-linked immunosorbent assay ; Enzymes ; Exposure ; Fish ; Genotoxicity ; Glutathione ; Glutathione peroxidase ; Heat shock ; Heat shock proteins ; Hsp70 protein ; Hydrogeology ; Insecticides ; Liver ; Marine fishes ; Oreochromis niloticus ; Organisms ; Organs ; Oxidative stress ; Parameters ; Peroxidase ; Pesticide toxicity ; Pesticides ; Proteins ; Risk assessment ; Soil Science & Conservation ; Spectrophotometry ; Stress proteins ; Superoxide ; Superoxide dismutase ; Thiamethoxam ; Thiobarbituric acid ; Tissue ; Toxicity ; Toxicity testing ; Toxicity tests ; Toxicology ; Water Quality/Water Pollution</subject><ispartof>Water, air, and soil pollution, 2021-06, Vol.232 (6), Article 221</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-38749d9eb817510f77bf57708ef049985bfb58d6f7efa847e118981acbd646283</citedby><cites>FETCH-LOGICAL-c386t-38749d9eb817510f77bf57708ef049985bfb58d6f7efa847e118981acbd646283</cites><orcidid>0000-0001-6559-4397 ; 0000-0003-0668-5744 ; 0000-0002-7393-730X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11270-021-05101-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11270-021-05101-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Temiz, Özge</creatorcontrib><creatorcontrib>Kargin, Dicle</creatorcontrib><creatorcontrib>Çogun, Hikmet Y</creatorcontrib><title>In Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam</title><title>Water, air, and soil pollution</title><addtitle>Water Air Soil Pollut</addtitle><description>This study aimed to observe the effect of toxicity of the pesticide thiamethoxam (TMX) at sublethal concentrations in the liver and brain of
Oreochromis niloticus
. In the experiment, fish were exposed to 50, 100, and, 150 mg/L with thiamethoxam for 48 h and 15 days. The superoxide dismutase (SOD), catalase (CAT), glutathione S-transferaz (GST), glutathione peroxidase (GPx), and ethoxyresorufin-
O
-deethylase (EROD) activities; and thiobarbituric acid reactive substance (TBARS), heat shock proteins 70 (HSP70), glutathione (GSH), and genotoxicity parameter 8-hydroxy-2′-deoxyguanosine (8-OHdG) were analyzed by spectrophotometric methods and ELISA techniques. Depending on time and dose in TMX exposure in liver tissue, a significant decrease in GSH level; an increase in SOD, GST, GPx, and EROD enzyme activities; and HSP70, TBARS, and 8-OHdG levels was determined. In brain tissue, SOD, GST, and EROD enzyme activities, an increase in HSP70, TBARS, and 8-OHdG levels, and a decrease in CAT enzyme activity and GSH levels were determined. In this study, TMX in the concentrations used showed that changes in oxidative stress biomarkers, genotoxicity parameter 8-OHdG levels, and HSP70 levels caused toxic effects in the model organism. As a result of the study, the changes and protective effects of the antioxidant system and stress proteins at the cellular level were determined in sublethal doses of toxic effects caused by TMX in the vital organs of the organism. In this toxicological study, TMX exposure resulted in toxicity to
O. niloticus
liver and brain tissues, in addition, responses of biomarkers to time and concentrations were determined. As a result of this study, the potential toxic effects of the commonly used pesticide TMX will reveal both the ecological risks of the aquatic organism and the basic data of the safety and risk assessments of
O. niloticus
consumed as food for human health.</description><subject>8-Hydroxydeoxyguanosine</subject><subject>Analysis</subject><subject>Antioxidants</subject><subject>Aquatic organisms</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Biocompatibility</subject><subject>Biological stress</subject><subject>Biomarkers</subject><subject>Body organs</subject><subject>Brain</subject><subject>Catalase</subject><subject>Chloramphenicol O-acetyltransferase</subject><subject>Climate Change/Climate Change Impacts</subject><subject>Deoxyguanosine</subject><subject>Earth and Environmental Science</subject><subject>ELISA</subject><subject>Environment</subject><subject>Environmental monitoring</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Exposure</subject><subject>Fish</subject><subject>Genotoxicity</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Heat shock</subject><subject>Heat shock proteins</subject><subject>Hsp70 protein</subject><subject>Hydrogeology</subject><subject>Insecticides</subject><subject>Liver</subject><subject>Marine fishes</subject><subject>Oreochromis niloticus</subject><subject>Organisms</subject><subject>Organs</subject><subject>Oxidative stress</subject><subject>Parameters</subject><subject>Peroxidase</subject><subject>Pesticide toxicity</subject><subject>Pesticides</subject><subject>Proteins</subject><subject>Risk assessment</subject><subject>Soil Science & Conservation</subject><subject>Spectrophotometry</subject><subject>Stress proteins</subject><subject>Superoxide</subject><subject>Superoxide dismutase</subject><subject>Thiamethoxam</subject><subject>Thiobarbituric acid</subject><subject>Tissue</subject><subject>Toxicity</subject><subject>Toxicity testing</subject><subject>Toxicity tests</subject><subject>Toxicology</subject><subject>Water Quality/Water 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Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam</title><author>Temiz, Özge ; Kargin, Dicle ; Çogun, Hikmet Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-38749d9eb817510f77bf57708ef049985bfb58d6f7efa847e118981acbd646283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>8-Hydroxydeoxyguanosine</topic><topic>Analysis</topic><topic>Antioxidants</topic><topic>Aquatic organisms</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Biocompatibility</topic><topic>Biological stress</topic><topic>Biomarkers</topic><topic>Body organs</topic><topic>Brain</topic><topic>Catalase</topic><topic>Chloramphenicol O-acetyltransferase</topic><topic>Climate Change/Climate Change Impacts</topic><topic>Deoxyguanosine</topic><topic>Earth and Environmental Science</topic><topic>ELISA</topic><topic>Environment</topic><topic>Environmental monitoring</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Exposure</topic><topic>Fish</topic><topic>Genotoxicity</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Heat shock</topic><topic>Heat shock proteins</topic><topic>Hsp70 protein</topic><topic>Hydrogeology</topic><topic>Insecticides</topic><topic>Liver</topic><topic>Marine fishes</topic><topic>Oreochromis niloticus</topic><topic>Organisms</topic><topic>Organs</topic><topic>Oxidative stress</topic><topic>Parameters</topic><topic>Peroxidase</topic><topic>Pesticide toxicity</topic><topic>Pesticides</topic><topic>Proteins</topic><topic>Risk assessment</topic><topic>Soil Science & Conservation</topic><topic>Spectrophotometry</topic><topic>Stress proteins</topic><topic>Superoxide</topic><topic>Superoxide dismutase</topic><topic>Thiamethoxam</topic><topic>Thiobarbituric acid</topic><topic>Tissue</topic><topic>Toxicity</topic><topic>Toxicity testing</topic><topic>Toxicity tests</topic><topic>Toxicology</topic><topic>Water Quality/Water Pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Temiz, Özge</creatorcontrib><creatorcontrib>Kargin, Dicle</creatorcontrib><creatorcontrib>Çogun, Hikmet Y</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ABI/INFORM Complete</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest - Health & Medical 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One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Water, air, and soil pollution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Temiz, Özge</au><au>Kargin, Dicle</au><au>Çogun, Hikmet Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam</atitle><jtitle>Water, air, and soil pollution</jtitle><stitle>Water Air Soil Pollut</stitle><date>2021-06-01</date><risdate>2021</risdate><volume>232</volume><issue>6</issue><artnum>221</artnum><issn>0049-6979</issn><eissn>1573-2932</eissn><abstract>This study aimed to observe the effect of toxicity of the pesticide thiamethoxam (TMX) at sublethal concentrations in the liver and brain of
Oreochromis niloticus
. In the experiment, fish were exposed to 50, 100, and, 150 mg/L with thiamethoxam for 48 h and 15 days. The superoxide dismutase (SOD), catalase (CAT), glutathione S-transferaz (GST), glutathione peroxidase (GPx), and ethoxyresorufin-
O
-deethylase (EROD) activities; and thiobarbituric acid reactive substance (TBARS), heat shock proteins 70 (HSP70), glutathione (GSH), and genotoxicity parameter 8-hydroxy-2′-deoxyguanosine (8-OHdG) were analyzed by spectrophotometric methods and ELISA techniques. Depending on time and dose in TMX exposure in liver tissue, a significant decrease in GSH level; an increase in SOD, GST, GPx, and EROD enzyme activities; and HSP70, TBARS, and 8-OHdG levels was determined. In brain tissue, SOD, GST, and EROD enzyme activities, an increase in HSP70, TBARS, and 8-OHdG levels, and a decrease in CAT enzyme activity and GSH levels were determined. In this study, TMX in the concentrations used showed that changes in oxidative stress biomarkers, genotoxicity parameter 8-OHdG levels, and HSP70 levels caused toxic effects in the model organism. As a result of the study, the changes and protective effects of the antioxidant system and stress proteins at the cellular level were determined in sublethal doses of toxic effects caused by TMX in the vital organs of the organism. In this toxicological study, TMX exposure resulted in toxicity to
O. niloticus
liver and brain tissues, in addition, responses of biomarkers to time and concentrations were determined. As a result of this study, the potential toxic effects of the commonly used pesticide TMX will reveal both the ecological risks of the aquatic organism and the basic data of the safety and risk assessments of
O. niloticus
consumed as food for human health.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s11270-021-05101-7</doi><orcidid>https://orcid.org/0000-0001-6559-4397</orcidid><orcidid>https://orcid.org/0000-0003-0668-5744</orcidid><orcidid>https://orcid.org/0000-0002-7393-730X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-6979 |
| ispartof | Water, air, and soil pollution, 2021-06, Vol.232 (6), Article 221 |
| issn | 0049-6979 1573-2932 |
| language | eng |
| recordid | cdi_proquest_journals_2528635387 |
| source | SpringerLink (Online service) |
| subjects | 8-Hydroxydeoxyguanosine Analysis Antioxidants Aquatic organisms Atmospheric Protection/Air Quality Control/Air Pollution Biocompatibility Biological stress Biomarkers Body organs Brain Catalase Chloramphenicol O-acetyltransferase Climate Change/Climate Change Impacts Deoxyguanosine Earth and Environmental Science ELISA Environment Environmental monitoring Enzymatic activity Enzyme activity Enzyme-linked immunosorbent assay Enzymes Exposure Fish Genotoxicity Glutathione Glutathione peroxidase Heat shock Heat shock proteins Hsp70 protein Hydrogeology Insecticides Liver Marine fishes Oreochromis niloticus Organisms Organs Oxidative stress Parameters Peroxidase Pesticide toxicity Pesticides Proteins Risk assessment Soil Science & Conservation Spectrophotometry Stress proteins Superoxide Superoxide dismutase Thiamethoxam Thiobarbituric acid Tissue Toxicity Toxicity testing Toxicity tests Toxicology Water Quality/Water Pollution |
| title | In Vivo Effects on Stress Protein, Genotoxicity, and Oxidative Toxicity Parameters in Oreochromis niloticus Tissues Exposed to Thiamethoxam |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A02%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20Vivo%20Effects%20on%20Stress%20Protein,%20Genotoxicity,%20and%20Oxidative%20Toxicity%20Parameters%20in%20Oreochromis%20niloticus%20Tissues%20Exposed%20to%20Thiamethoxam&rft.jtitle=Water,%20air,%20and%20soil%20pollution&rft.au=Temiz,%20%C3%96zge&rft.date=2021-06-01&rft.volume=232&rft.issue=6&rft.artnum=221&rft.issn=0049-6979&rft.eissn=1573-2932&rft_id=info:doi/10.1007/s11270-021-05101-7&rft_dat=%3Cgale_proqu%3EA662385896%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2528635387&rft_id=info:pmid/&rft_galeid=A662385896&rfr_iscdi=true |