Dilution of nutrient elements formulation in culture media for in vitro conservation of Coffea arabica AS2K variety

In vitro conservation technology is one of the strategies to maintain the genetic diversity of perennial plants, such as Arabica coffee. The purpose of this study was to examine the effect of nutrient elements dilution on the emphasis of growth of Arabica coffee micro cuttings of AS2K variety. The plant materials were Arabica coffee plantlets of AS2K variety which were reproduced using Murashige and Skoog (MS) media and given BA 2 g L −1 , sucrose 30 g L −1 , and phytagel 2.5 g L −1 . The treatment conservation used MS medium without adding growth regulator with sucrose 30 g L −1 and phytagel 2.5 g L −1 . The examined treatment was the dilution of MS nutrient elements into a half (1/2) and a quarter (1/4) of the basic concentration, the full (basic) MS as the control. The experimental design used was a completely randomized design with 10 replications. The results showed that Arabica coffee culture on 1/2 MS media can be considered for conservation since it is cost-effective, able to reduce the growth of shoots and produce more rooted plantlets than controls. This media culture can be stored for 8 months without morphological changes so that it can recover spontaneously.