Construction of a recombinant vector containing SAI gene using Gateway-based RNAi system: a preliminary study in the development of high sucrose sugarcane

Recombinant plasmid construction that produces hairpin molecules for gene knockdown is not an easy method. Gateway cloning system is a technology to transfer DNA fragments utilizing clonase and specific sites. This study aimed to construct RNAi expression vector containing soluble acid invertase ( S...

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Veröffentlicht in:	IOP conference series. Earth and environmental science 2021-05, Vol.762 (1), p.12068
Hauptverfasser:	Hadiarto, T, Riyanti, E I, Listanto, E, Polosoro, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Amplification Cloning Construction Deoxyribonucleic acid DNA Fragments Invertase Nucleotide sequence RNA-mediated interference Sucrose Sugarcane Technology transfer Thymine
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