Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria
Aims The nitrogen-fixing symbiosis fixation between legumes and Rhizobium helps the plant to survive and to compete effectively on nitrogen poor soils. The soil environment attached to the root system is characterized by intense microbial activity. This work aimed to study the diversity of indigenou...
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| Veröffentlicht in: | Plant and soil 2021-04, Vol.461 (1/2), p.369-388 |
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| creator | Gaci, Meriem Benhizia, Yacine Dems, Mohamed AbdEsselem Boukaous, Leyla Benguedouar, Ammar Hynes, Michael Francis |
| description | Aims
The nitrogen-fixing symbiosis fixation between legumes and
Rhizobium
helps the plant to survive and to compete effectively on nitrogen poor soils. The soil environment attached to the root system is characterized by intense microbial activity. This work aimed to study the diversity of indigenous rhizobia living in the rhizosphere of forage pea in eastern and central Algeria.
Methods
Bacteria were trapped in vitro from collected soils. They were isolated from nodules by direct isolation or by bacterial trapping, and tested for nodulation. Further characterization was carried out to identify the isolates.
Results
Ninety-four isolates were obtained. Thirty-four were rhizobial strains that could, with two exceptions, establish root-nodule symbioses with the host plant. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the
Rhizobium leguminosarum
group of species. The strains revealed diverse plasmid profiles, and diverse restriction patterns of
cpn60
gene amplicons and on Southern blot analysis of
nodABC
genes. Sequences of 16S rRNA gene showed that all isolates exhibited 99.85% identity with
R. leguminosarum
sv.
viciae
USDA 2370
T
,
R. laguerreae
FB206
T
and
R. anhuiense
CCBAU 23252
T
. Analysis of
cpn60
sequences revealed two groups. One of them clustered with ≤99% identity with
R. laguerreae
FB403. The second was clustered with ≤99% identity with
R. laguerreae
FB206
T
.
Conclusion
Our findings suggest that the isolates may belong to the species
R. laguerreae
. |
| doi_str_mv | 10.1007/s11104-020-04795-8 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_journals_2522237071</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>27292655</jstor_id><sourcerecordid>27292655</sourcerecordid><originalsourceid>FETCH-LOGICAL-c341t-cc897267305f6d82391136cd4918a9d6821b05fd5fd9fdab5953857c4d5cf2993</originalsourceid><addsrcrecordid>eNp9kFtLAzEQhYMoWKt_QBACPm_NZbPZPJbiDQoWUfAtpEm2TWk3NckW6qO_3NQVfRMGhmHOd4Y5AFxiNMII8ZuIMUZlgQgqUMkFK-ojMMCM04IhWh2DAUKUFIiLt1NwFuMKHWZcDcDnbLlf-4VtbXIaGrezIbq0h76BrjUuL3wX4fPSffi56zYwBbXdWgOb4POwtLBVqQtqDZdq7pJKB3DmYlZGldwu9-koO0GrYrKhhao1UNs2HZDxemGDU-fgpFHraC9--hC83t2-TB6K6dP942Q8LTQtcSq0rgUnFaeINZWpCRUY00qbUuBaCVPVBM_zyuQSjVFzJhitGdelYbohQtAhuO59t8G_dzYmufJdaPNJSRghhHLEcVaRXqWDjzHYRm6D26iwlxjJQ9ayz1rmrOV31rLOEO2hmMVt_urP-l_qqqdWMfnwe4dwIkjFGP0CceaMzQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2522237071</pqid></control><display><type>article</type><title>Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria</title><source>Springer Nature - Complete Springer Journals</source><creator>Gaci, Meriem ; Benhizia, Yacine ; Dems, Mohamed AbdEsselem ; Boukaous, Leyla ; Benguedouar, Ammar ; Hynes, Michael Francis</creator><creatorcontrib>Gaci, Meriem ; Benhizia, Yacine ; Dems, Mohamed AbdEsselem ; Boukaous, Leyla ; Benguedouar, Ammar ; Hynes, Michael Francis</creatorcontrib><description>Aims
The nitrogen-fixing symbiosis fixation between legumes and
Rhizobium
helps the plant to survive and to compete effectively on nitrogen poor soils. The soil environment attached to the root system is characterized by intense microbial activity. This work aimed to study the diversity of indigenous rhizobia living in the rhizosphere of forage pea in eastern and central Algeria.
Methods
Bacteria were trapped in vitro from collected soils. They were isolated from nodules by direct isolation or by bacterial trapping, and tested for nodulation. Further characterization was carried out to identify the isolates.
Results
Ninety-four isolates were obtained. Thirty-four were rhizobial strains that could, with two exceptions, establish root-nodule symbioses with the host plant. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the
Rhizobium leguminosarum
group of species. The strains revealed diverse plasmid profiles, and diverse restriction patterns of
cpn60
gene amplicons and on Southern blot analysis of
nodABC
genes. Sequences of 16S rRNA gene showed that all isolates exhibited 99.85% identity with
R. leguminosarum
sv.
viciae
USDA 2370
T
,
R. laguerreae
FB206
T
and
R. anhuiense
CCBAU 23252
T
. Analysis of
cpn60
sequences revealed two groups. One of them clustered with ≤99% identity with
R. laguerreae
FB403. The second was clustered with ≤99% identity with
R. laguerreae
FB206
T
.
Conclusion
Our findings suggest that the isolates may belong to the species
R. laguerreae
.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/s11104-020-04795-8</identifier><language>eng</language><publisher>Cham: Springer Science + Business Media</publisher><subject>Bacteria ; Biological activity ; Biomedical and Life Sciences ; Ecology ; Foraging habitats ; Genes ; Host plants ; Legumes ; Life Sciences ; Microbial activity ; Microorganisms ; Nitrogen ; Nitrogen fixation ; Nitrogenation ; Nodulation ; Nodules ; Phylogenetics ; Phylogeny ; Plant Physiology ; Plant Sciences ; Plasmids ; Regular Article ; REGULAR ARTICLES ; Rhizosphere ; rRNA 16S ; Soil environment ; Soil Science & Conservation ; Soils ; Strains (organisms) ; Symbiosis</subject><ispartof>Plant and soil, 2021-04, Vol.461 (1/2), p.369-388</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-cc897267305f6d82391136cd4918a9d6821b05fd5fd9fdab5953857c4d5cf2993</citedby><cites>FETCH-LOGICAL-c341t-cc897267305f6d82391136cd4918a9d6821b05fd5fd9fdab5953857c4d5cf2993</cites><orcidid>0000-0002-3608-2971</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11104-020-04795-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11104-020-04795-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Gaci, Meriem</creatorcontrib><creatorcontrib>Benhizia, Yacine</creatorcontrib><creatorcontrib>Dems, Mohamed AbdEsselem</creatorcontrib><creatorcontrib>Boukaous, Leyla</creatorcontrib><creatorcontrib>Benguedouar, Ammar</creatorcontrib><creatorcontrib>Hynes, Michael Francis</creatorcontrib><title>Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria</title><title>Plant and soil</title><addtitle>Plant Soil</addtitle><description>Aims
The nitrogen-fixing symbiosis fixation between legumes and
Rhizobium
helps the plant to survive and to compete effectively on nitrogen poor soils. The soil environment attached to the root system is characterized by intense microbial activity. This work aimed to study the diversity of indigenous rhizobia living in the rhizosphere of forage pea in eastern and central Algeria.
Methods
Bacteria were trapped in vitro from collected soils. They were isolated from nodules by direct isolation or by bacterial trapping, and tested for nodulation. Further characterization was carried out to identify the isolates.
Results
Ninety-four isolates were obtained. Thirty-four were rhizobial strains that could, with two exceptions, establish root-nodule symbioses with the host plant. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the
Rhizobium leguminosarum
group of species. The strains revealed diverse plasmid profiles, and diverse restriction patterns of
cpn60
gene amplicons and on Southern blot analysis of
nodABC
genes. Sequences of 16S rRNA gene showed that all isolates exhibited 99.85% identity with
R. leguminosarum
sv.
viciae
USDA 2370
T
,
R. laguerreae
FB206
T
and
R. anhuiense
CCBAU 23252
T
. Analysis of
cpn60
sequences revealed two groups. One of them clustered with ≤99% identity with
R. laguerreae
FB403. The second was clustered with ≤99% identity with
R. laguerreae
FB206
T
.
Conclusion
Our findings suggest that the isolates may belong to the species
R. laguerreae
.</description><subject>Bacteria</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Ecology</subject><subject>Foraging habitats</subject><subject>Genes</subject><subject>Host plants</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Microbial activity</subject><subject>Microorganisms</subject><subject>Nitrogen</subject><subject>Nitrogen fixation</subject><subject>Nitrogenation</subject><subject>Nodulation</subject><subject>Nodules</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plasmids</subject><subject>Regular Article</subject><subject>REGULAR ARTICLES</subject><subject>Rhizosphere</subject><subject>rRNA 16S</subject><subject>Soil environment</subject><subject>Soil Science & Conservation</subject><subject>Soils</subject><subject>Strains (organisms)</subject><subject>Symbiosis</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kFtLAzEQhYMoWKt_QBACPm_NZbPZPJbiDQoWUfAtpEm2TWk3NckW6qO_3NQVfRMGhmHOd4Y5AFxiNMII8ZuIMUZlgQgqUMkFK-ojMMCM04IhWh2DAUKUFIiLt1NwFuMKHWZcDcDnbLlf-4VtbXIaGrezIbq0h76BrjUuL3wX4fPSffi56zYwBbXdWgOb4POwtLBVqQtqDZdq7pJKB3DmYlZGldwu9-koO0GrYrKhhao1UNs2HZDxemGDU-fgpFHraC9--hC83t2-TB6K6dP942Q8LTQtcSq0rgUnFaeINZWpCRUY00qbUuBaCVPVBM_zyuQSjVFzJhitGdelYbohQtAhuO59t8G_dzYmufJdaPNJSRghhHLEcVaRXqWDjzHYRm6D26iwlxjJQ9ayz1rmrOV31rLOEO2hmMVt_urP-l_qqqdWMfnwe4dwIkjFGP0CceaMzQ</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Gaci, Meriem</creator><creator>Benhizia, Yacine</creator><creator>Dems, Mohamed AbdEsselem</creator><creator>Boukaous, Leyla</creator><creator>Benguedouar, Ammar</creator><creator>Hynes, Michael Francis</creator><general>Springer Science + Business Media</general><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7X2</scope><scope>88A</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-3608-2971</orcidid></search><sort><creationdate>20210401</creationdate><title>Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria</title><author>Gaci, Meriem ; Benhizia, Yacine ; Dems, Mohamed AbdEsselem ; Boukaous, Leyla ; Benguedouar, Ammar ; Hynes, Michael Francis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-cc897267305f6d82391136cd4918a9d6821b05fd5fd9fdab5953857c4d5cf2993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacteria</topic><topic>Biological activity</topic><topic>Biomedical and Life Sciences</topic><topic>Ecology</topic><topic>Foraging habitats</topic><topic>Genes</topic><topic>Host plants</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Microbial activity</topic><topic>Microorganisms</topic><topic>Nitrogen</topic><topic>Nitrogen fixation</topic><topic>Nitrogenation</topic><topic>Nodulation</topic><topic>Nodules</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plasmids</topic><topic>Regular Article</topic><topic>REGULAR ARTICLES</topic><topic>Rhizosphere</topic><topic>rRNA 16S</topic><topic>Soil environment</topic><topic>Soil Science & Conservation</topic><topic>Soils</topic><topic>Strains (organisms)</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaci, Meriem</creatorcontrib><creatorcontrib>Benhizia, Yacine</creatorcontrib><creatorcontrib>Dems, Mohamed AbdEsselem</creatorcontrib><creatorcontrib>Boukaous, Leyla</creatorcontrib><creatorcontrib>Benguedouar, Ammar</creatorcontrib><creatorcontrib>Hynes, Michael Francis</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaci, Meriem</au><au>Benhizia, Yacine</au><au>Dems, Mohamed AbdEsselem</au><au>Boukaous, Leyla</au><au>Benguedouar, Ammar</au><au>Hynes, Michael Francis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria</atitle><jtitle>Plant and soil</jtitle><stitle>Plant Soil</stitle><date>2021-04-01</date><risdate>2021</risdate><volume>461</volume><issue>1/2</issue><spage>369</spage><epage>388</epage><pages>369-388</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><abstract>Aims
The nitrogen-fixing symbiosis fixation between legumes and
Rhizobium
helps the plant to survive and to compete effectively on nitrogen poor soils. The soil environment attached to the root system is characterized by intense microbial activity. This work aimed to study the diversity of indigenous rhizobia living in the rhizosphere of forage pea in eastern and central Algeria.
Methods
Bacteria were trapped in vitro from collected soils. They were isolated from nodules by direct isolation or by bacterial trapping, and tested for nodulation. Further characterization was carried out to identify the isolates.
Results
Ninety-four isolates were obtained. Thirty-four were rhizobial strains that could, with two exceptions, establish root-nodule symbioses with the host plant. Different profiles of susceptibility to rhizobiophages indicated that the majority of isolates were likely to be members of the
Rhizobium leguminosarum
group of species. The strains revealed diverse plasmid profiles, and diverse restriction patterns of
cpn60
gene amplicons and on Southern blot analysis of
nodABC
genes. Sequences of 16S rRNA gene showed that all isolates exhibited 99.85% identity with
R. leguminosarum
sv.
viciae
USDA 2370
T
,
R. laguerreae
FB206
T
and
R. anhuiense
CCBAU 23252
T
. Analysis of
cpn60
sequences revealed two groups. One of them clustered with ≤99% identity with
R. laguerreae
FB403. The second was clustered with ≤99% identity with
R. laguerreae
FB206
T
.
Conclusion
Our findings suggest that the isolates may belong to the species
R. laguerreae
.</abstract><cop>Cham</cop><pub>Springer Science + Business Media</pub><doi>10.1007/s11104-020-04795-8</doi><tpages>20</tpages><orcidid>https://orcid.org/0000-0002-3608-2971</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-079X |
| ispartof | Plant and soil, 2021-04, Vol.461 (1/2), p.369-388 |
| issn | 0032-079X 1573-5036 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Bacteria Biological activity Biomedical and Life Sciences Ecology Foraging habitats Genes Host plants Legumes Life Sciences Microbial activity Microorganisms Nitrogen Nitrogen fixation Nitrogenation Nodulation Nodules Phylogenetics Phylogeny Plant Physiology Plant Sciences Plasmids Regular Article REGULAR ARTICLES Rhizosphere rRNA 16S Soil environment Soil Science & Conservation Soils Strains (organisms) Symbiosis |
| title | Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria |
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