A high‐throughput genetically directed protein crosslinking analysis reveals the physiological relevance of the ATP synthase ‘inserted’ state

We developed a large scale in vivo protein photocrosslinking analysis pipeline based on the introduction of unnatural amino acid into target protein via scarless genome‐targeted site‐directed mutagenesis, and probing cross‐linked products via high‐throughput polyacrylamide gel electrophoresis. We un...

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Veröffentlicht in:	The FEBS journal 2021-05, Vol.288 (9), p.2989-3009
Hauptverfasser:	Liu, Yang, Yu, Jiayu, Wang, Mengyuan, Zeng, Qingfang, Fu, Xinmiao, Chang, Zengyi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - genetics Adenosine Triphosphate - metabolism Amino Acid Sequence - genetics Amino acids Amino Acids - genetics ATP ATP synthase ATPase Inhibitory Protein Bacteria Crosslinking Electrophoresis Energy Metabolism - genetics Environmental changes Environmental conditions Escherichia coli - enzymology Gel electrophoresis Genomes genome‐based site‐directed mutagenesis high‐throughput PAGE Multiprotein Complexes - genetics Multiprotein Complexes - ultrastructure Mutagenesis, Site-Directed photo‐cross‐linking Physiology Polyacrylamide Protein Conformation Protein interaction Protein Subunits - genetics Proteins Proteins - genetics Proteins - ultrastructure Proton-Translocating ATPases - genetics Proton-Translocating ATPases - ultrastructure Site-directed mutagenesis Subunit structure unnatural amino acid
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