A panel of 96 SNPs to identify individuals and differentiate subspecies of elk (Cervus canadensis) for microfluidic and other genotyping platforms
The tule elk ( Cervus canadensis nannodes ) is endemic to California, USA, where it experienced an extreme genetic bottleneck in the 1870s. Two other subspecies of elk co-occur in California, Roosevelt ( C . c. roosevelti ) and Rocky Mountain ( C. c. nelsoni ) elk. These subspecies potentially inter...
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| Veröffentlicht in: | Conservation genetics resources 2021-06, Vol.13 (2), p.111-113 |
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| creator | Sacks, Benjamin N. Bali, Gunmeet K. Lounsberry, Zachary T. Vanderzwan, Stevi L. |
| description | The tule elk (
Cervus canadensis nannodes
) is endemic to California, USA, where it experienced an extreme genetic bottleneck in the 1870s. Two other subspecies of elk co-occur in California, Roosevelt (
C
. c.
roosevelti
) and Rocky Mountain (
C. c. nelsoni
) elk. These subspecies potentially interbreed in contact zones. For monitoring and genetic management of fragmented populations, markers are needed to differentiate both individuals and subspecies. Using Illumina HiSeq sequencing of 12 individuals, including four from each subspecies, we developed a suite of 96 SNP markers for a Fluidigm platform. This marker set enabled differentiation of individuals and subspecies with high confidence and will facilitate genetic monitoring of elk throughout their range. |
| doi_str_mv | 10.1007/s12686-020-01184-2 |
| format | Article |
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Cervus canadensis nannodes
) is endemic to California, USA, where it experienced an extreme genetic bottleneck in the 1870s. Two other subspecies of elk co-occur in California, Roosevelt (
C
. c.
roosevelti
) and Rocky Mountain (
C. c. nelsoni
) elk. These subspecies potentially interbreed in contact zones. For monitoring and genetic management of fragmented populations, markers are needed to differentiate both individuals and subspecies. Using Illumina HiSeq sequencing of 12 individuals, including four from each subspecies, we developed a suite of 96 SNP markers for a Fluidigm platform. This marker set enabled differentiation of individuals and subspecies with high confidence and will facilitate genetic monitoring of elk throughout their range.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-020-01184-2</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Genetics and Genomics ; Biodiversity ; Biomedical and Life Sciences ; Cervus canadensis ; Cervus elaphus ; Conservation Biology/Ecology ; Ecology ; Evolutionary Biology ; Genotyping ; Life Sciences ; Microfluidics ; Plant Genetics and Genomics ; Population genetics ; Single-nucleotide polymorphism ; Technical Note</subject><ispartof>Conservation genetics resources, 2021-06, Vol.13 (2), p.111-113</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-ebda5f81fd46b089a24688cfe343825cc5f180ed248ea4b32225a135197171303</citedby><cites>FETCH-LOGICAL-c319t-ebda5f81fd46b089a24688cfe343825cc5f180ed248ea4b32225a135197171303</cites><orcidid>0000-0003-0143-6589</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-020-01184-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-020-01184-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Sacks, Benjamin N.</creatorcontrib><creatorcontrib>Bali, Gunmeet K.</creatorcontrib><creatorcontrib>Lounsberry, Zachary T.</creatorcontrib><creatorcontrib>Vanderzwan, Stevi L.</creatorcontrib><title>A panel of 96 SNPs to identify individuals and differentiate subspecies of elk (Cervus canadensis) for microfluidic and other genotyping platforms</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>The tule elk (
Cervus canadensis nannodes
) is endemic to California, USA, where it experienced an extreme genetic bottleneck in the 1870s. Two other subspecies of elk co-occur in California, Roosevelt (
C
. c.
roosevelti
) and Rocky Mountain (
C. c. nelsoni
) elk. These subspecies potentially interbreed in contact zones. For monitoring and genetic management of fragmented populations, markers are needed to differentiate both individuals and subspecies. Using Illumina HiSeq sequencing of 12 individuals, including four from each subspecies, we developed a suite of 96 SNP markers for a Fluidigm platform. This marker set enabled differentiation of individuals and subspecies with high confidence and will facilitate genetic monitoring of elk throughout their range.</description><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Cervus canadensis</subject><subject>Cervus elaphus</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Genotyping</subject><subject>Life Sciences</subject><subject>Microfluidics</subject><subject>Plant Genetics and Genomics</subject><subject>Population genetics</subject><subject>Single-nucleotide polymorphism</subject><subject>Technical Note</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMtOwzAQRSMEEqXwA6wssYFFwI_EcZZVxUuqAAlYW048Li6pE-ykUn-DL8YlCHasZqS5987MSZJTgi8JxsVVIJQLnmKKU0yIyFK6l0yIKIq0oBzv__Y5PUyOQlhhzAWjdJJ8zlCnHDSoNajk6PnhKaC-RVaD663ZIuu03Vg9qCYg5TTS1hjwu6HqAYWhCh3UFsLOD807Op-D3wwB1cqpmBFsuECm9Whta9-aZrDa1t9Bbf8GHi3Btf22s26Jukb1UbkOx8mBievg5KdOk9eb65f5Xbp4vL2fzxZpzUjZp1BplRtBjM54hUWpaMaFqA2wjAma13VuiMCgaSZAZVX8luaKsJyUBSkIw2yanI25nW8_Bgi9XLWDd3GlpDkpeMExZlFFR1W8PwQPRnberpXfSoLljr0c2cvIXn6zlzSa2GgKUeyW4P-i_3F9AcF2iFQ</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Sacks, Benjamin N.</creator><creator>Bali, Gunmeet K.</creator><creator>Lounsberry, Zachary T.</creator><creator>Vanderzwan, Stevi L.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0003-0143-6589</orcidid></search><sort><creationdate>20210601</creationdate><title>A panel of 96 SNPs to identify individuals and differentiate subspecies of elk (Cervus canadensis) for microfluidic and other genotyping platforms</title><author>Sacks, Benjamin N. ; Bali, Gunmeet K. ; Lounsberry, Zachary T. ; Vanderzwan, Stevi L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-ebda5f81fd46b089a24688cfe343825cc5f180ed248ea4b32225a135197171303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Cervus canadensis</topic><topic>Cervus elaphus</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Genotyping</topic><topic>Life Sciences</topic><topic>Microfluidics</topic><topic>Plant Genetics and Genomics</topic><topic>Population genetics</topic><topic>Single-nucleotide polymorphism</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sacks, Benjamin N.</creatorcontrib><creatorcontrib>Bali, Gunmeet K.</creatorcontrib><creatorcontrib>Lounsberry, Zachary T.</creatorcontrib><creatorcontrib>Vanderzwan, Stevi L.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sacks, Benjamin N.</au><au>Bali, Gunmeet K.</au><au>Lounsberry, Zachary T.</au><au>Vanderzwan, Stevi L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A panel of 96 SNPs to identify individuals and differentiate subspecies of elk (Cervus canadensis) for microfluidic and other genotyping platforms</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2021-06-01</date><risdate>2021</risdate><volume>13</volume><issue>2</issue><spage>111</spage><epage>113</epage><pages>111-113</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>The tule elk (
Cervus canadensis nannodes
) is endemic to California, USA, where it experienced an extreme genetic bottleneck in the 1870s. Two other subspecies of elk co-occur in California, Roosevelt (
C
. c.
roosevelti
) and Rocky Mountain (
C. c. nelsoni
) elk. These subspecies potentially interbreed in contact zones. For monitoring and genetic management of fragmented populations, markers are needed to differentiate both individuals and subspecies. Using Illumina HiSeq sequencing of 12 individuals, including four from each subspecies, we developed a suite of 96 SNP markers for a Fluidigm platform. This marker set enabled differentiation of individuals and subspecies with high confidence and will facilitate genetic monitoring of elk throughout their range.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-020-01184-2</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0003-0143-6589</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1877-7252 |
| ispartof | Conservation genetics resources, 2021-06, Vol.13 (2), p.111-113 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Cervus canadensis Cervus elaphus Conservation Biology/Ecology Ecology Evolutionary Biology Genotyping Life Sciences Microfluidics Plant Genetics and Genomics Population genetics Single-nucleotide polymorphism Technical Note |
| title | A panel of 96 SNPs to identify individuals and differentiate subspecies of elk (Cervus canadensis) for microfluidic and other genotyping platforms |
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