Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata
The tobacco N gene encodes the N resistance factor that recognizes a tobacco mosaic virus elicitor called p50 to induce a cell-death-associated hypersensitive reaction, which confines virus infections to local areas. Coexpression of N and p50 cDNAs in Nicotiana benthamiana induces resistance against...
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| Veröffentlicht in: | Journal of general plant pathology : JGPP 2021, Vol.87 (3), p.170-177 |
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| container_title | Journal of general plant pathology : JGPP |
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| creator | Sasaki, Nobumitsu Murakami, Tomoya Yoshimoto, Nanae Komatsu, Ken Matsushita, Yasuhiko Nyunoya, Hiroshi |
| description | The tobacco
N
gene encodes the N resistance factor that recognizes a tobacco mosaic virus elicitor called p50 to induce a cell-death-associated hypersensitive reaction, which confines virus infections to local areas. Coexpression of
N
and
p50
cDNAs in
Nicotiana benthamiana
induces resistance against virus infection without visible cell death. In this study, we examined how this N-mediated resistance in the heterologous plant inhibits virus infection. After inoculation with GFP-encoding tomato mosaic virus (ToMV), plants coexpressing
N
and
p50
had fewer and smaller infection sites as the levels of coexpressed proteins of N and p50 increased. This antiviral response was associated with production of reactive oxygen species but not ion leakage or expression of
PR1a
(encoding a salicylic acid signaling marker). In addition, it was likely that GFP-tagged movement protein (MP) of ToMV seemed to have failed to localize at plasmodesmata during virus infection in leaves coexpressing
N
and
p50
. These findings indicate that the cell death-independent, N-mediated antiviral response in
N. benthamiana
may involve interference with the intracellular transport function of MP. |
| doi_str_mv | 10.1007/s10327-021-00984-0 |
| format | Article |
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N
gene encodes the N resistance factor that recognizes a tobacco mosaic virus elicitor called p50 to induce a cell-death-associated hypersensitive reaction, which confines virus infections to local areas. Coexpression of
N
and
p50
cDNAs in
Nicotiana benthamiana
induces resistance against virus infection without visible cell death. In this study, we examined how this N-mediated resistance in the heterologous plant inhibits virus infection. After inoculation with GFP-encoding tomato mosaic virus (ToMV), plants coexpressing
N
and
p50
had fewer and smaller infection sites as the levels of coexpressed proteins of N and p50 increased. This antiviral response was associated with production of reactive oxygen species but not ion leakage or expression of
PR1a
(encoding a salicylic acid signaling marker). In addition, it was likely that GFP-tagged movement protein (MP) of ToMV seemed to have failed to localize at plasmodesmata during virus infection in leaves coexpressing
N
and
p50
. These findings indicate that the cell death-independent, N-mediated antiviral response in
N. benthamiana
may involve interference with the intracellular transport function of MP.</description><identifier>ISSN: 1345-2630</identifier><identifier>EISSN: 1610-739X</identifier><identifier>DOI: 10.1007/s10327-021-00984-0</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Agriculture ; Antiviral drugs ; Apoptosis ; Biomedical and Life Sciences ; Cell death ; Disease resistance ; Infections ; Inoculation ; Life Sciences ; Localization ; Microbiology ; Mortality ; Movement protein ; N gene ; Nicotiana benthamiana ; Plant Pathology ; Plant virus diseases ; Plasmodesmata ; Proteins ; Reactive oxygen species ; Resistance factors ; Salicylic acid ; Tobacco ; Tomatoes ; Viral and Viroid Diseases ; Viruses</subject><ispartof>Journal of general plant pathology : JGPP, 2021, Vol.87 (3), p.170-177</ispartof><rights>The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2021</rights><rights>The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p157t-3d93830b42d709aa8153bd46fbdaf66c93bbe323496ccedfdcb6a39268738f1d3</cites><orcidid>0000-0001-9202-5149</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10327-021-00984-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10327-021-00984-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Sasaki, Nobumitsu</creatorcontrib><creatorcontrib>Murakami, Tomoya</creatorcontrib><creatorcontrib>Yoshimoto, Nanae</creatorcontrib><creatorcontrib>Komatsu, Ken</creatorcontrib><creatorcontrib>Matsushita, Yasuhiko</creatorcontrib><creatorcontrib>Nyunoya, Hiroshi</creatorcontrib><title>Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata</title><title>Journal of general plant pathology : JGPP</title><addtitle>J Gen Plant Pathol</addtitle><description>The tobacco
N
gene encodes the N resistance factor that recognizes a tobacco mosaic virus elicitor called p50 to induce a cell-death-associated hypersensitive reaction, which confines virus infections to local areas. Coexpression of
N
and
p50
cDNAs in
Nicotiana benthamiana
induces resistance against virus infection without visible cell death. In this study, we examined how this N-mediated resistance in the heterologous plant inhibits virus infection. After inoculation with GFP-encoding tomato mosaic virus (ToMV), plants coexpressing
N
and
p50
had fewer and smaller infection sites as the levels of coexpressed proteins of N and p50 increased. This antiviral response was associated with production of reactive oxygen species but not ion leakage or expression of
PR1a
(encoding a salicylic acid signaling marker). In addition, it was likely that GFP-tagged movement protein (MP) of ToMV seemed to have failed to localize at plasmodesmata during virus infection in leaves coexpressing
N
and
p50
. These findings indicate that the cell death-independent, N-mediated antiviral response in
N. benthamiana
may involve interference with the intracellular transport function of MP.</description><subject>Agriculture</subject><subject>Antiviral drugs</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Cell death</subject><subject>Disease resistance</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Microbiology</subject><subject>Mortality</subject><subject>Movement protein</subject><subject>N gene</subject><subject>Nicotiana benthamiana</subject><subject>Plant Pathology</subject><subject>Plant virus diseases</subject><subject>Plasmodesmata</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Resistance factors</subject><subject>Salicylic acid</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Viral and Viroid Diseases</subject><subject>Viruses</subject><issn>1345-2630</issn><issn>1610-739X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpFUcFq3DAQNSWFbrb9gZ4EOSuRNLZsH8vSJoFlc0mhNzOyxl0ttuRY2oXkt_qD1WYLvcx7DI83j3lF8VWKWylEfRelAFVzoSQXom1KLj4UK6ml4DW0v64yh7LiSoP4VFzHeBBZCXW1Kv5saBy5JUx77rylmfLwiaFP7uQWHNlCcQ4-EpvIOkxkmXllu_PaxYS-JzZgn8LCnGc714fk0CMz2WSP0zt3_hTGE8VM9s64s8UYehzdGyYXPAsDS8HgFPLBY2QZaTpnmJeQKLumwOYR4xQsxQkTfi4-DjhG-vIP18XPH9-fNw98-3T_uPm25bOs6sTBttCAMKWytWgRG1mBsaUejMVB674FYwgUlK3ue7KD7Y1GaJVuamgGaWFd3Fx8c5CXI8XUHcJx8flkpyqplZagqqyCiyrOi_O_afmvkqI7t9Nd2unyz7v3djoBfwFyvYkA</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Sasaki, Nobumitsu</creator><creator>Murakami, Tomoya</creator><creator>Yoshimoto, Nanae</creator><creator>Komatsu, Ken</creator><creator>Matsushita, Yasuhiko</creator><creator>Nyunoya, Hiroshi</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0001-9202-5149</orcidid></search><sort><creationdate>2021</creationdate><title>Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata</title><author>Sasaki, Nobumitsu ; Murakami, Tomoya ; Yoshimoto, Nanae ; Komatsu, Ken ; Matsushita, Yasuhiko ; Nyunoya, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p157t-3d93830b42d709aa8153bd46fbdaf66c93bbe323496ccedfdcb6a39268738f1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agriculture</topic><topic>Antiviral drugs</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Cell death</topic><topic>Disease resistance</topic><topic>Infections</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Microbiology</topic><topic>Mortality</topic><topic>Movement protein</topic><topic>N gene</topic><topic>Nicotiana benthamiana</topic><topic>Plant Pathology</topic><topic>Plant virus diseases</topic><topic>Plasmodesmata</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Resistance factors</topic><topic>Salicylic acid</topic><topic>Tobacco</topic><topic>Tomatoes</topic><topic>Viral and Viroid Diseases</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, Nobumitsu</creatorcontrib><creatorcontrib>Murakami, Tomoya</creatorcontrib><creatorcontrib>Yoshimoto, Nanae</creatorcontrib><creatorcontrib>Komatsu, Ken</creatorcontrib><creatorcontrib>Matsushita, Yasuhiko</creatorcontrib><creatorcontrib>Nyunoya, Hiroshi</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of general plant pathology : JGPP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sasaki, Nobumitsu</au><au>Murakami, Tomoya</au><au>Yoshimoto, Nanae</au><au>Komatsu, Ken</au><au>Matsushita, Yasuhiko</au><au>Nyunoya, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata</atitle><jtitle>Journal of general plant pathology : JGPP</jtitle><stitle>J Gen Plant Pathol</stitle><date>2021</date><risdate>2021</risdate><volume>87</volume><issue>3</issue><spage>170</spage><epage>177</epage><pages>170-177</pages><issn>1345-2630</issn><eissn>1610-739X</eissn><abstract>The tobacco
N
gene encodes the N resistance factor that recognizes a tobacco mosaic virus elicitor called p50 to induce a cell-death-associated hypersensitive reaction, which confines virus infections to local areas. Coexpression of
N
and
p50
cDNAs in
Nicotiana benthamiana
induces resistance against virus infection without visible cell death. In this study, we examined how this N-mediated resistance in the heterologous plant inhibits virus infection. After inoculation with GFP-encoding tomato mosaic virus (ToMV), plants coexpressing
N
and
p50
had fewer and smaller infection sites as the levels of coexpressed proteins of N and p50 increased. This antiviral response was associated with production of reactive oxygen species but not ion leakage or expression of
PR1a
(encoding a salicylic acid signaling marker). In addition, it was likely that GFP-tagged movement protein (MP) of ToMV seemed to have failed to localize at plasmodesmata during virus infection in leaves coexpressing
N
and
p50
. These findings indicate that the cell death-independent, N-mediated antiviral response in
N. benthamiana
may involve interference with the intracellular transport function of MP.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s10327-021-00984-0</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9202-5149</orcidid></addata></record> |
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| ispartof | Journal of general plant pathology : JGPP, 2021, Vol.87 (3), p.170-177 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Agriculture Antiviral drugs Apoptosis Biomedical and Life Sciences Cell death Disease resistance Infections Inoculation Life Sciences Localization Microbiology Mortality Movement protein N gene Nicotiana benthamiana Plant Pathology Plant virus diseases Plasmodesmata Proteins Reactive oxygen species Resistance factors Salicylic acid Tobacco Tomatoes Viral and Viroid Diseases Viruses |
| title | Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata |
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