PsGRGH, a TOS1 family-like gene, is involved in the vegetative growth, environmental stress response, and pathogenicity of Phytophthora sojae
Phytophthora sojae can cause soybean phytophthora blight, and greatly reduce soybean production. It is helpful to understand the pathogenic mechanism of P. sojae to prevent soybean phytophthora blight and ensures food security. Glycosyl hydrolases (GHs) disintegrate plant cell walls for nutrition an...
Gespeichert in:
| Veröffentlicht in: | European journal of plant pathology 2021-05, Vol.160 (1), p.67-79 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 79 |
|---|---|
| container_issue | 1 |
| container_start_page | 67 |
| container_title | European journal of plant pathology |
| container_volume | 160 |
| creator | Liu, Dong Li, Kunyuan Hu, Jiulong Li, Ping Liu, Xiao Gao, Zhimou |
| description | Phytophthora sojae
can cause soybean phytophthora blight, and greatly reduce soybean production. It is helpful to understand the pathogenic mechanism of
P. sojae
to prevent soybean phytophthora blight and ensures food security. Glycosyl hydrolases (GHs) disintegrate plant cell walls for nutrition and invasion, and they may act as an important virulence factor during
P. sojae
infection. To reveal the role of GHs in the main stages of
P. sojae
life cycle, we measured the expression level of genes, which encoded GHs, and constructed
P. sojae
transformants for biological function verification by the gene editing technology CRISPR-Cas9. In this study, four genes, encoded special GH proteins, with conserved glycine-rich were identified in the
P. sojae
genome, and their sequences were similar to those of the TOS1 family genes. One of them, the xp_009520987.1, was named
PsGRGH,
and was significantly upregulated during the germination of spores and in the early infection stages. The results revealed that
PsGRGH
was involved in the growth and mycelial morphology regulation in
P. sojae
, and was essential for its sporangium development and virulence. In addition,
PsGRGH
is localized on the cell membrane and plays an important role in tolerance toward
Bacillus
and abiotic stress. |
| doi_str_mv | 10.1007/s10658-021-02221-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2513091503</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2513091503</sourcerecordid><originalsourceid>FETCH-LOGICAL-c319t-66ea9516fdfb742638cf3a6cd0c1f5198ce4490612294ab7c9fb781a2f20055e3</originalsourceid><addsrcrecordid>eNp9kMtKAzEUhoMoWC8v4CrgtqO5zC1LKdoKQsXLOsTpSSd1moxJHJmH8J2NVnDn4lw4fP9_4EfojJILSkh1GSgpizojjKZiqY97aEKLimd1Xop9NCGCiYzWFT9ERyFsSBIJwSbo8z7MH-aLKVb4aflIsVZb041ZZ14Br8HCFJuAjR1cN8AqLTi2gAdYQ1TRDInx7iO2Uwx2MN7ZLdioOhyihxBwar2zIZkou8K9iq1LnqYxccRO4_t2jK5v09UrHNxGwQk60KoLcPo7j9HzzfXTbJHdLee3s6u7rOFUxKwsQYmClnqlX6qclbxuNFdlsyIN1QUVdQN5LkhJGRO5eqkakbiaKqYZIUUB_Bid73x7797eIUS5ce_eppeSFZQTQQvCE8V2VONdCB607L3ZKj9KSuR37HIXu0yxy5_Y5ZhEfCcKCbZr8H_W_6i-AAPNh28</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2513091503</pqid></control><display><type>article</type><title>PsGRGH, a TOS1 family-like gene, is involved in the vegetative growth, environmental stress response, and pathogenicity of Phytophthora sojae</title><source>SpringerLink Journals - AutoHoldings</source><creator>Liu, Dong ; Li, Kunyuan ; Hu, Jiulong ; Li, Ping ; Liu, Xiao ; Gao, Zhimou</creator><creatorcontrib>Liu, Dong ; Li, Kunyuan ; Hu, Jiulong ; Li, Ping ; Liu, Xiao ; Gao, Zhimou</creatorcontrib><description>Phytophthora sojae
can cause soybean phytophthora blight, and greatly reduce soybean production. It is helpful to understand the pathogenic mechanism of
P. sojae
to prevent soybean phytophthora blight and ensures food security. Glycosyl hydrolases (GHs) disintegrate plant cell walls for nutrition and invasion, and they may act as an important virulence factor during
P. sojae
infection. To reveal the role of GHs in the main stages of
P. sojae
life cycle, we measured the expression level of genes, which encoded GHs, and constructed
P. sojae
transformants for biological function verification by the gene editing technology CRISPR-Cas9. In this study, four genes, encoded special GH proteins, with conserved glycine-rich were identified in the
P. sojae
genome, and their sequences were similar to those of the TOS1 family genes. One of them, the xp_009520987.1, was named
PsGRGH,
and was significantly upregulated during the germination of spores and in the early infection stages. The results revealed that
PsGRGH
was involved in the growth and mycelial morphology regulation in
P. sojae
, and was essential for its sporangium development and virulence. In addition,
PsGRGH
is localized on the cell membrane and plays an important role in tolerance toward
Bacillus
and abiotic stress.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-021-02221-y</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Biomedical and Life Sciences ; Blight ; Cell membranes ; Cell walls ; CRISPR ; Crop production ; Ecology ; Environmental stress ; Food security ; Gene expression ; Gene sequencing ; Genes ; Genetic modification ; Genomes ; Germination ; Glycine ; Glycosidases ; Glycosyl hydrolase ; Life cycles ; Life Sciences ; Morphology ; Mycelia ; Nutrition ; Pathogenicity ; Pathogens ; Phytophthora sojae ; Plant Pathology ; Plant Sciences ; Soybeans ; Spores ; Virulence ; Virulence factors</subject><ispartof>European journal of plant pathology, 2021-05, Vol.160 (1), p.67-79</ispartof><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2021</rights><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-66ea9516fdfb742638cf3a6cd0c1f5198ce4490612294ab7c9fb781a2f20055e3</citedby><cites>FETCH-LOGICAL-c319t-66ea9516fdfb742638cf3a6cd0c1f5198ce4490612294ab7c9fb781a2f20055e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-021-02221-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-021-02221-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41466,42535,51296</link.rule.ids></links><search><creatorcontrib>Liu, Dong</creatorcontrib><creatorcontrib>Li, Kunyuan</creatorcontrib><creatorcontrib>Hu, Jiulong</creatorcontrib><creatorcontrib>Li, Ping</creatorcontrib><creatorcontrib>Liu, Xiao</creatorcontrib><creatorcontrib>Gao, Zhimou</creatorcontrib><title>PsGRGH, a TOS1 family-like gene, is involved in the vegetative growth, environmental stress response, and pathogenicity of Phytophthora sojae</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>Phytophthora sojae
can cause soybean phytophthora blight, and greatly reduce soybean production. It is helpful to understand the pathogenic mechanism of
P. sojae
to prevent soybean phytophthora blight and ensures food security. Glycosyl hydrolases (GHs) disintegrate plant cell walls for nutrition and invasion, and they may act as an important virulence factor during
P. sojae
infection. To reveal the role of GHs in the main stages of
P. sojae
life cycle, we measured the expression level of genes, which encoded GHs, and constructed
P. sojae
transformants for biological function verification by the gene editing technology CRISPR-Cas9. In this study, four genes, encoded special GH proteins, with conserved glycine-rich were identified in the
P. sojae
genome, and their sequences were similar to those of the TOS1 family genes. One of them, the xp_009520987.1, was named
PsGRGH,
and was significantly upregulated during the germination of spores and in the early infection stages. The results revealed that
PsGRGH
was involved in the growth and mycelial morphology regulation in
P. sojae
, and was essential for its sporangium development and virulence. In addition,
PsGRGH
is localized on the cell membrane and plays an important role in tolerance toward
Bacillus
and abiotic stress.</description><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>CRISPR</subject><subject>Crop production</subject><subject>Ecology</subject><subject>Environmental stress</subject><subject>Food security</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic modification</subject><subject>Genomes</subject><subject>Germination</subject><subject>Glycine</subject><subject>Glycosidases</subject><subject>Glycosyl hydrolase</subject><subject>Life cycles</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Mycelia</subject><subject>Nutrition</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Phytophthora sojae</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Soybeans</subject><subject>Spores</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kMtKAzEUhoMoWC8v4CrgtqO5zC1LKdoKQsXLOsTpSSd1moxJHJmH8J2NVnDn4lw4fP9_4EfojJILSkh1GSgpizojjKZiqY97aEKLimd1Xop9NCGCiYzWFT9ERyFsSBIJwSbo8z7MH-aLKVb4aflIsVZb041ZZ14Br8HCFJuAjR1cN8AqLTi2gAdYQ1TRDInx7iO2Uwx2MN7ZLdioOhyihxBwar2zIZkou8K9iq1LnqYxccRO4_t2jK5v09UrHNxGwQk60KoLcPo7j9HzzfXTbJHdLee3s6u7rOFUxKwsQYmClnqlX6qclbxuNFdlsyIN1QUVdQN5LkhJGRO5eqkakbiaKqYZIUUB_Bid73x7797eIUS5ce_eppeSFZQTQQvCE8V2VONdCB607L3ZKj9KSuR37HIXu0yxy5_Y5ZhEfCcKCbZr8H_W_6i-AAPNh28</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Liu, Dong</creator><creator>Li, Kunyuan</creator><creator>Hu, Jiulong</creator><creator>Li, Ping</creator><creator>Liu, Xiao</creator><creator>Gao, Zhimou</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20210501</creationdate><title>PsGRGH, a TOS1 family-like gene, is involved in the vegetative growth, environmental stress response, and pathogenicity of Phytophthora sojae</title><author>Liu, Dong ; Li, Kunyuan ; Hu, Jiulong ; Li, Ping ; Liu, Xiao ; Gao, Zhimou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-66ea9516fdfb742638cf3a6cd0c1f5198ce4490612294ab7c9fb781a2f20055e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agriculture</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>Cell membranes</topic><topic>Cell walls</topic><topic>CRISPR</topic><topic>Crop production</topic><topic>Ecology</topic><topic>Environmental stress</topic><topic>Food security</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic modification</topic><topic>Genomes</topic><topic>Germination</topic><topic>Glycine</topic><topic>Glycosidases</topic><topic>Glycosyl hydrolase</topic><topic>Life cycles</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Mycelia</topic><topic>Nutrition</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Phytophthora sojae</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Soybeans</topic><topic>Spores</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Dong</creatorcontrib><creatorcontrib>Li, Kunyuan</creatorcontrib><creatorcontrib>Hu, Jiulong</creatorcontrib><creatorcontrib>Li, Ping</creatorcontrib><creatorcontrib>Liu, Xiao</creatorcontrib><creatorcontrib>Gao, Zhimou</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Dong</au><au>Li, Kunyuan</au><au>Hu, Jiulong</au><au>Li, Ping</au><au>Liu, Xiao</au><au>Gao, Zhimou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PsGRGH, a TOS1 family-like gene, is involved in the vegetative growth, environmental stress response, and pathogenicity of Phytophthora sojae</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2021-05-01</date><risdate>2021</risdate><volume>160</volume><issue>1</issue><spage>67</spage><epage>79</epage><pages>67-79</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>Phytophthora sojae
can cause soybean phytophthora blight, and greatly reduce soybean production. It is helpful to understand the pathogenic mechanism of
P. sojae
to prevent soybean phytophthora blight and ensures food security. Glycosyl hydrolases (GHs) disintegrate plant cell walls for nutrition and invasion, and they may act as an important virulence factor during
P. sojae
infection. To reveal the role of GHs in the main stages of
P. sojae
life cycle, we measured the expression level of genes, which encoded GHs, and constructed
P. sojae
transformants for biological function verification by the gene editing technology CRISPR-Cas9. In this study, four genes, encoded special GH proteins, with conserved glycine-rich were identified in the
P. sojae
genome, and their sequences were similar to those of the TOS1 family genes. One of them, the xp_009520987.1, was named
PsGRGH,
and was significantly upregulated during the germination of spores and in the early infection stages. The results revealed that
PsGRGH
was involved in the growth and mycelial morphology regulation in
P. sojae
, and was essential for its sporangium development and virulence. In addition,
PsGRGH
is localized on the cell membrane and plays an important role in tolerance toward
Bacillus
and abiotic stress.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10658-021-02221-y</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0929-1873 |
| ispartof | European journal of plant pathology, 2021-05, Vol.160 (1), p.67-79 |
| issn | 0929-1873 1573-8469 |
| language | eng |
| recordid | cdi_proquest_journals_2513091503 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Biomedical and Life Sciences Blight Cell membranes Cell walls CRISPR Crop production Ecology Environmental stress Food security Gene expression Gene sequencing Genes Genetic modification Genomes Germination Glycine Glycosidases Glycosyl hydrolase Life cycles Life Sciences Morphology Mycelia Nutrition Pathogenicity Pathogens Phytophthora sojae Plant Pathology Plant Sciences Soybeans Spores Virulence Virulence factors |
| title | PsGRGH, a TOS1 family-like gene, is involved in the vegetative growth, environmental stress response, and pathogenicity of Phytophthora sojae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T08%3A21%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PsGRGH,%20a%20TOS1%20family-like%20gene,%20is%20involved%20in%20the%20vegetative%20growth,%20environmental%20stress%20response,%20and%20pathogenicity%20of%20Phytophthora%20sojae&rft.jtitle=European%20journal%20of%20plant%20pathology&rft.au=Liu,%20Dong&rft.date=2021-05-01&rft.volume=160&rft.issue=1&rft.spage=67&rft.epage=79&rft.pages=67-79&rft.issn=0929-1873&rft.eissn=1573-8469&rft_id=info:doi/10.1007/s10658-021-02221-y&rft_dat=%3Cproquest_cross%3E2513091503%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2513091503&rft_id=info:pmid/&rfr_iscdi=true |