Bacillus tequilensis Isolated from Fermented Intestine of Holothuria Scabra Produces Fibrinolytic Protease with Thrombolysis Activity
Among essential treatment of cardiovascular disorders are fibrinolytic proteases. Most thrombolysis agents are fibrinolytic enzymes from group of bacterial proteases. This work reports a potent bacterium isolated from fermented intestine of H. scabra , which could produce fibrinolytic protease with...
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| Veröffentlicht in: | IOP conference series. Earth and environmental science 2021-03, Vol.707 (1), p.12008 |
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| creator | Hidayati, Nur Nurrahman, Nurrahman Fuad, Hayatun Munandar, Hendra Zilda, Dewi Seswita Ernanto, Aditya Rahman Samiasih, Amin Oedjijono, Oedjijono Ethica, Stalis Norma |
| description | Among essential treatment of cardiovascular disorders are fibrinolytic proteases. Most thrombolysis agents are fibrinolytic enzymes from group of bacterial proteases. This work reports a potent bacterium isolated from fermented intestine of
H. scabra
, which could produce fibrinolytic protease with high thrombolysis activity. Bacterial selection was conducted based on proteolytic and fibrinolytic activities indicated as clear zone on skim milk and fibrin agar media, respectively. Crude proteases from the selected bacterial isolates were subjected to thrombolytic activity test based on gravimetric method, which results were confirmed after 7 repetitions. As result, 4 fibrinolytic protease-producing bacterial isolates HFSI-3, HFSI-4, HFSI-5 and HFSI-8 were obtained. Among them, HFSI-5 isolate identified as
Bacillus tequilensis
on the basis of the 16S rRNA gene sequencing and morphological properties produced crude protease with the highest thrombolytic activity. The thrombolytic activity of crude protease produced by
B. tequilensis
HFSI-5 is worthy of comparing to that of standard fibrinolytic enzyme Nattokinase showing its potential as thrombolysis agent. |
| doi_str_mv | 10.1088/1755-1315/707/1/012008 |
| format | Article |
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H. scabra
, which could produce fibrinolytic protease with high thrombolysis activity. Bacterial selection was conducted based on proteolytic and fibrinolytic activities indicated as clear zone on skim milk and fibrin agar media, respectively. Crude proteases from the selected bacterial isolates were subjected to thrombolytic activity test based on gravimetric method, which results were confirmed after 7 repetitions. As result, 4 fibrinolytic protease-producing bacterial isolates HFSI-3, HFSI-4, HFSI-5 and HFSI-8 were obtained. Among them, HFSI-5 isolate identified as
Bacillus tequilensis
on the basis of the 16S rRNA gene sequencing and morphological properties produced crude protease with the highest thrombolytic activity. The thrombolytic activity of crude protease produced by
B. tequilensis
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H. scabra
, which could produce fibrinolytic protease with high thrombolysis activity. Bacterial selection was conducted based on proteolytic and fibrinolytic activities indicated as clear zone on skim milk and fibrin agar media, respectively. Crude proteases from the selected bacterial isolates were subjected to thrombolytic activity test based on gravimetric method, which results were confirmed after 7 repetitions. As result, 4 fibrinolytic protease-producing bacterial isolates HFSI-3, HFSI-4, HFSI-5 and HFSI-8 were obtained. Among them, HFSI-5 isolate identified as
Bacillus tequilensis
on the basis of the 16S rRNA gene sequencing and morphological properties produced crude protease with the highest thrombolytic activity. The thrombolytic activity of crude protease produced by
B. tequilensis
HFSI-5 is worthy of comparing to that of standard fibrinolytic enzyme Nattokinase showing its potential as thrombolysis agent.</description><subject>Bacillus tequilensis</subject><subject>Bacteria</subject><subject>Fibrin</subject><subject>Gene sequencing</subject><subject>Gravimetry</subject><subject>Intestine</subject><subject>Protease</subject><subject>Proteinase</subject><subject>Proteolysis</subject><subject>rRNA 16S</subject><subject>Skim milk</subject><subject>Thrombolysis</subject><issn>1755-1307</issn><issn>1755-1315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo9kN9KwzAUxosoOKevIAGva3OatGkv53BuMFBwXoc0PWUZXbMlqbIH8L1tmezm_PsO3-H8ougR6DPQokhAZFkMDLJEUJFAQiGltLiKJhfh-lJTcRvdeb-jNBeclZPo90Vp07a9JwGPvWmx88aTlbetCliTxtk9WaDbYze2qyH6YDoktiFL29qw7Z1R5FOryiny4Wzda_RkYSpnOtuegtHjNKDySH5M2JLNdrCsBmm8M9PBfJtwuo9uGtV6fPjP0-hr8bqZL-P1-9tqPlvHOi1YEedaC8yhqTWA1mWtGIdK84rWaVlXdVpghYoLAcU4SbnOGJYInItS5GXD2DR6OvsenD32wytyZ3vXDSdlmkFaZjnP-bCVn7e0s947bOTBmb1yJwlUjsjlSFOOZOWAXII8I2d_vsN3cw</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Hidayati, Nur</creator><creator>Nurrahman, Nurrahman</creator><creator>Fuad, Hayatun</creator><creator>Munandar, Hendra</creator><creator>Zilda, Dewi Seswita</creator><creator>Ernanto, Aditya Rahman</creator><creator>Samiasih, Amin</creator><creator>Oedjijono, Oedjijono</creator><creator>Ethica, Stalis Norma</creator><general>IOP Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope></search><sort><creationdate>20210301</creationdate><title>Bacillus tequilensis Isolated from Fermented Intestine of Holothuria Scabra Produces Fibrinolytic Protease with Thrombolysis Activity</title><author>Hidayati, Nur ; Nurrahman, Nurrahman ; Fuad, Hayatun ; Munandar, Hendra ; Zilda, Dewi Seswita ; Ernanto, Aditya Rahman ; Samiasih, Amin ; Oedjijono, Oedjijono ; Ethica, Stalis Norma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2838-6cc7e61fdc11cc9da341bc4b0d29dbd28ebea477180d2924c53e9e14479769f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bacillus tequilensis</topic><topic>Bacteria</topic><topic>Fibrin</topic><topic>Gene sequencing</topic><topic>Gravimetry</topic><topic>Intestine</topic><topic>Protease</topic><topic>Proteinase</topic><topic>Proteolysis</topic><topic>rRNA 16S</topic><topic>Skim milk</topic><topic>Thrombolysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hidayati, Nur</creatorcontrib><creatorcontrib>Nurrahman, Nurrahman</creatorcontrib><creatorcontrib>Fuad, Hayatun</creatorcontrib><creatorcontrib>Munandar, Hendra</creatorcontrib><creatorcontrib>Zilda, Dewi Seswita</creatorcontrib><creatorcontrib>Ernanto, Aditya Rahman</creatorcontrib><creatorcontrib>Samiasih, Amin</creatorcontrib><creatorcontrib>Oedjijono, Oedjijono</creatorcontrib><creatorcontrib>Ethica, Stalis Norma</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><jtitle>IOP conference series. Earth and environmental science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hidayati, Nur</au><au>Nurrahman, Nurrahman</au><au>Fuad, Hayatun</au><au>Munandar, Hendra</au><au>Zilda, Dewi Seswita</au><au>Ernanto, Aditya Rahman</au><au>Samiasih, Amin</au><au>Oedjijono, Oedjijono</au><au>Ethica, Stalis Norma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacillus tequilensis Isolated from Fermented Intestine of Holothuria Scabra Produces Fibrinolytic Protease with Thrombolysis Activity</atitle><jtitle>IOP conference series. Earth and environmental science</jtitle><date>2021-03-01</date><risdate>2021</risdate><volume>707</volume><issue>1</issue><spage>12008</spage><pages>12008-</pages><issn>1755-1307</issn><eissn>1755-1315</eissn><abstract>Among essential treatment of cardiovascular disorders are fibrinolytic proteases. Most thrombolysis agents are fibrinolytic enzymes from group of bacterial proteases. This work reports a potent bacterium isolated from fermented intestine of
H. scabra
, which could produce fibrinolytic protease with high thrombolysis activity. Bacterial selection was conducted based on proteolytic and fibrinolytic activities indicated as clear zone on skim milk and fibrin agar media, respectively. Crude proteases from the selected bacterial isolates were subjected to thrombolytic activity test based on gravimetric method, which results were confirmed after 7 repetitions. As result, 4 fibrinolytic protease-producing bacterial isolates HFSI-3, HFSI-4, HFSI-5 and HFSI-8 were obtained. Among them, HFSI-5 isolate identified as
Bacillus tequilensis
on the basis of the 16S rRNA gene sequencing and morphological properties produced crude protease with the highest thrombolytic activity. The thrombolytic activity of crude protease produced by
B. tequilensis
HFSI-5 is worthy of comparing to that of standard fibrinolytic enzyme Nattokinase showing its potential as thrombolysis agent.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1755-1315/707/1/012008</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus tequilensis Bacteria Fibrin Gene sequencing Gravimetry Intestine Protease Proteinase Proteolysis rRNA 16S Skim milk Thrombolysis |
| title | Bacillus tequilensis Isolated from Fermented Intestine of Holothuria Scabra Produces Fibrinolytic Protease with Thrombolysis Activity |
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