Determination of Total Tannins and Antibacterial Activities Ethanol Extraction Seri (Muntingia calabura L.) Leaves
This research aims were to determine the total tannin level and to test the antibacterial activity of the ethanol extract of Muntingia calabura L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric...
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| Veröffentlicht in: | Journal of physics. Conference series 2021-03, Vol.1811 (1), p.12121 |
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| creator | Gurning, K Simanjuntak, H A Purba, H Situmorang, R F R Barus, L Silaban, S |
| description | This research aims were to determine the total tannin level and to test the antibacterial activity of the ethanol extract of
Muntingia calabura
L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric method which was measured at a wavelength of 745 nm using UV-Vis spectrometry and tannic acid was used as the standard. The antibacterial activity test was carried out by diffusion disc method against, such as
Escherichia coli
,
Salmonella typhi
and
Propionebacterium acnes
bacteria. The variations in the concentration of the ethanol extract of
M. calabura
included 12.5%; 25%; 50%, and 75%. Chloramphenicol was used as positive control and DMSO 10% as negative control. The results showed that the ethanol extract of the
M. calabura
leaves had a total tannin level of 0.0655±0.0002 mg/g d.w (Mean±SD). Antibacterial activity of ethanol extraction by the
M. calabura
leaves against
E. coli
(14.18±0.96; 15.53±0.40; 15.92±1.27; and 16.50±0.52),
S. typhi
(13.37±0.35; 14.47±1.14; 14.97±0.87; and 15.50±0.66), and
P. acnes
(14.13±0.24; 14.60±0.20; 15.52±1.14; and 16.37±0.46). Antibacterial activity of Chloramphenicol against
E. coli
,
S. typhi
and
P. acnes
are 31.25±2.08; 25.15±1.61; and 23.25±4.42. |
| doi_str_mv | 10.1088/1742-6596/1811/1/012121 |
| format | Article |
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Muntingia calabura
L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric method which was measured at a wavelength of 745 nm using UV-Vis spectrometry and tannic acid was used as the standard. The antibacterial activity test was carried out by diffusion disc method against, such as
Escherichia coli
,
Salmonella typhi
and
Propionebacterium acnes
bacteria. The variations in the concentration of the ethanol extract of
M. calabura
included 12.5%; 25%; 50%, and 75%. Chloramphenicol was used as positive control and DMSO 10% as negative control. The results showed that the ethanol extract of the
M. calabura
leaves had a total tannin level of 0.0655±0.0002 mg/g d.w (Mean±SD). Antibacterial activity of ethanol extraction by the
M. calabura
leaves against
E. coli
(14.18±0.96; 15.53±0.40; 15.92±1.27; and 16.50±0.52),
S. typhi
(13.37±0.35; 14.47±1.14; 14.97±0.87; and 15.50±0.66), and
P. acnes
(14.13±0.24; 14.60±0.20; 15.52±1.14; and 16.37±0.46). Antibacterial activity of Chloramphenicol against
E. coli
,
S. typhi
and
P. acnes
are 31.25±2.08; 25.15±1.61; and 23.25±4.42.</description><identifier>ISSN: 1742-6588</identifier><identifier>EISSN: 1742-6596</identifier><identifier>DOI: 10.1088/1742-6596/1811/1/012121</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Chloromycetin ; Colorimetry ; E coli ; Ethanol ; Physics ; Salmonella ; Tannic acid ; Tannins</subject><ispartof>Journal of physics. Conference series, 2021-03, Vol.1811 (1), p.12121</ispartof><rights>2021. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2861-b79000c6d654b20804a2919037957d9804954fddb6437b541bf220b2f1e1c7b23</citedby><cites>FETCH-LOGICAL-c2861-b79000c6d654b20804a2919037957d9804954fddb6437b541bf220b2f1e1c7b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Gurning, K</creatorcontrib><creatorcontrib>Simanjuntak, H A</creatorcontrib><creatorcontrib>Purba, H</creatorcontrib><creatorcontrib>Situmorang, R F R</creatorcontrib><creatorcontrib>Barus, L</creatorcontrib><creatorcontrib>Silaban, S</creatorcontrib><title>Determination of Total Tannins and Antibacterial Activities Ethanol Extraction Seri (Muntingia calabura L.) Leaves</title><title>Journal of physics. Conference series</title><description>This research aims were to determine the total tannin level and to test the antibacterial activity of the ethanol extract of
Muntingia calabura
L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric method which was measured at a wavelength of 745 nm using UV-Vis spectrometry and tannic acid was used as the standard. The antibacterial activity test was carried out by diffusion disc method against, such as
Escherichia coli
,
Salmonella typhi
and
Propionebacterium acnes
bacteria. The variations in the concentration of the ethanol extract of
M. calabura
included 12.5%; 25%; 50%, and 75%. Chloramphenicol was used as positive control and DMSO 10% as negative control. The results showed that the ethanol extract of the
M. calabura
leaves had a total tannin level of 0.0655±0.0002 mg/g d.w (Mean±SD). Antibacterial activity of ethanol extraction by the
M. calabura
leaves against
E. coli
(14.18±0.96; 15.53±0.40; 15.92±1.27; and 16.50±0.52),
S. typhi
(13.37±0.35; 14.47±1.14; 14.97±0.87; and 15.50±0.66), and
P. acnes
(14.13±0.24; 14.60±0.20; 15.52±1.14; and 16.37±0.46). Antibacterial activity of Chloramphenicol against
E. coli
,
S. typhi
and
P. acnes
are 31.25±2.08; 25.15±1.61; and 23.25±4.42.</description><subject>Chloromycetin</subject><subject>Colorimetry</subject><subject>E coli</subject><subject>Ethanol</subject><subject>Physics</subject><subject>Salmonella</subject><subject>Tannic acid</subject><subject>Tannins</subject><issn>1742-6588</issn><issn>1742-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNo9kMtOwzAQRS0EEqXwDVhiA4s0HufheFlBeUhBLChry04ccJU6xXYq-HscFXVmMR7dO9fSQegayAJIVaXAcpqUBS9TqABSSAnQ2CdodlROj--qOkcX3m8IyWKxGXIPOmi3NVYGM1g8dHg9BNnjtbTWWI-lbfHSBqNkE30mKssmmL0JRnu8Cl_SDj1e_QQX9SngPZrw7esYT-ynkbiRvVSjk7he3OFay732l-isk73XV_9zjj4eV-v756R-e3q5X9ZJQ6sSEsU4IaQp27LIFSUVySXlwEnGeMFaHnde5F3bqjLPmCpyUB2lRNEONDRM0WyObg65Ozd8j9oHsRlGZ-OXghYQs0oeGcwRO7gaN3jvdCd2zmyl-xVAxARYTOjEhFFMgAWIA-DsD-hxbUs</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Gurning, K</creator><creator>Simanjuntak, H A</creator><creator>Purba, H</creator><creator>Situmorang, R F R</creator><creator>Barus, L</creator><creator>Silaban, S</creator><general>IOP Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>L7M</scope><scope>P5Z</scope><scope>P62</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20210301</creationdate><title>Determination of Total Tannins and Antibacterial Activities Ethanol Extraction Seri (Muntingia calabura L.) Leaves</title><author>Gurning, K ; Simanjuntak, H A ; Purba, H ; Situmorang, R F R ; Barus, L ; Silaban, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2861-b79000c6d654b20804a2919037957d9804954fddb6437b541bf220b2f1e1c7b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Chloromycetin</topic><topic>Colorimetry</topic><topic>E coli</topic><topic>Ethanol</topic><topic>Physics</topic><topic>Salmonella</topic><topic>Tannic acid</topic><topic>Tannins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gurning, K</creatorcontrib><creatorcontrib>Simanjuntak, H A</creatorcontrib><creatorcontrib>Purba, H</creatorcontrib><creatorcontrib>Situmorang, R F R</creatorcontrib><creatorcontrib>Barus, L</creatorcontrib><creatorcontrib>Silaban, S</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of physics. Conference series</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gurning, K</au><au>Simanjuntak, H A</au><au>Purba, H</au><au>Situmorang, R F R</au><au>Barus, L</au><au>Silaban, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Total Tannins and Antibacterial Activities Ethanol Extraction Seri (Muntingia calabura L.) Leaves</atitle><jtitle>Journal of physics. Conference series</jtitle><date>2021-03-01</date><risdate>2021</risdate><volume>1811</volume><issue>1</issue><spage>12121</spage><pages>12121-</pages><issn>1742-6588</issn><eissn>1742-6596</eissn><abstract>This research aims were to determine the total tannin level and to test the antibacterial activity of the ethanol extract of
Muntingia calabura
L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric method which was measured at a wavelength of 745 nm using UV-Vis spectrometry and tannic acid was used as the standard. The antibacterial activity test was carried out by diffusion disc method against, such as
Escherichia coli
,
Salmonella typhi
and
Propionebacterium acnes
bacteria. The variations in the concentration of the ethanol extract of
M. calabura
included 12.5%; 25%; 50%, and 75%. Chloramphenicol was used as positive control and DMSO 10% as negative control. The results showed that the ethanol extract of the
M. calabura
leaves had a total tannin level of 0.0655±0.0002 mg/g d.w (Mean±SD). Antibacterial activity of ethanol extraction by the
M. calabura
leaves against
E. coli
(14.18±0.96; 15.53±0.40; 15.92±1.27; and 16.50±0.52),
S. typhi
(13.37±0.35; 14.47±1.14; 14.97±0.87; and 15.50±0.66), and
P. acnes
(14.13±0.24; 14.60±0.20; 15.52±1.14; and 16.37±0.46). Antibacterial activity of Chloramphenicol against
E. coli
,
S. typhi
and
P. acnes
are 31.25±2.08; 25.15±1.61; and 23.25±4.42.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><doi>10.1088/1742-6596/1811/1/012121</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Chloromycetin Colorimetry E coli Ethanol Physics Salmonella Tannic acid Tannins |
| title | Determination of Total Tannins and Antibacterial Activities Ethanol Extraction Seri (Muntingia calabura L.) Leaves |
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