Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification
Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification...
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| Veröffentlicht in: | Waste and biomass valorization 2021-05, Vol.12 (5), p.2521-2539 |
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| creator | Teixeira, Wéllen Fabrícia Aviz Batista, Ryhára Dias do Amaral Santos, Claudia Cristina Auler Júnior, Aloísio Chagas Freitas Terrasan, César Rafael Fanchini de Santana, Marcio Waluce Pinheiro R. de Siqueira, Félix Gonçalves de Paula-Elias, Fabrício Coutinho de Almeida, Alex Fernando |
| description | Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of
Trichoderma
strains with
Aspergillus niger
or
Pleurotus ostreatus
were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of
Trichoderma asperellum
PEC-6 and
P. ostreatus
increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions.
T. asperellum
PEC-17 and
A. niger
increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production.
Graphic Abstract |
| doi_str_mv | 10.1007/s12649-020-01199-8 |
| format | Article |
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Trichoderma
strains with
Aspergillus niger
or
Pleurotus ostreatus
were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of
Trichoderma asperellum
PEC-6 and
P. ostreatus
increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions.
T. asperellum
PEC-17 and
A. niger
increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production.
Graphic Abstract</description><identifier>ISSN: 1877-2641</identifier><identifier>EISSN: 1877-265X</identifier><identifier>DOI: 10.1007/s12649-020-01199-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agricultural wastes ; Biorefineries ; Cellobiase ; Cellulose ; Consortia ; Cultivation ; Endoglucanase ; Engineering ; Environment ; Environmental Engineering/Biotechnology ; Enzymes ; Fruits ; Fungi ; Glucosidase ; Industrial Pollution Prevention ; Lignocellulose ; Monoculture ; Operating costs ; Original Paper ; Pectinase ; Pineapples ; Refining ; Renewable and Green Energy ; Saccharification ; Solid state ; Sugar ; Waste Management/Waste Technology ; Wastes ; Xylanase ; β-Glucosidase</subject><ispartof>Waste and biomass valorization, 2021-05, Vol.12 (5), p.2521-2539</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-16b0e06c189fe24067ac5fcdb585193c278ce1c5499ec901f0b1aad8b8c401eb3</citedby><cites>FETCH-LOGICAL-c356t-16b0e06c189fe24067ac5fcdb585193c278ce1c5499ec901f0b1aad8b8c401eb3</cites><orcidid>0000-0003-2397-3318 ; 0000-0002-7489-8701 ; 0000-0001-5512-6119 ; 0000-0001-5239-4994 ; 0000-0002-7988-4755 ; 0000-0001-8095-2156 ; 0000-0003-3753-7568 ; 0000-0001-5391-4621</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12649-020-01199-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12649-020-01199-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Teixeira, Wéllen Fabrícia Aviz</creatorcontrib><creatorcontrib>Batista, Ryhára Dias</creatorcontrib><creatorcontrib>do Amaral Santos, Claudia Cristina Auler</creatorcontrib><creatorcontrib>Júnior, Aloísio Chagas Freitas</creatorcontrib><creatorcontrib>Terrasan, César Rafael Fanchini</creatorcontrib><creatorcontrib>de Santana, Marcio Waluce Pinheiro R.</creatorcontrib><creatorcontrib>de Siqueira, Félix Gonçalves</creatorcontrib><creatorcontrib>de Paula-Elias, Fabrício Coutinho</creatorcontrib><creatorcontrib>de Almeida, Alex Fernando</creatorcontrib><title>Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification</title><title>Waste and biomass valorization</title><addtitle>Waste Biomass Valor</addtitle><description>Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of
Trichoderma
strains with
Aspergillus niger
or
Pleurotus ostreatus
were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of
Trichoderma asperellum
PEC-6 and
P. ostreatus
increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions.
T. asperellum
PEC-17 and
A. niger
increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production.
Graphic Abstract</description><subject>Agricultural wastes</subject><subject>Biorefineries</subject><subject>Cellobiase</subject><subject>Cellulose</subject><subject>Consortia</subject><subject>Cultivation</subject><subject>Endoglucanase</subject><subject>Engineering</subject><subject>Environment</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymes</subject><subject>Fruits</subject><subject>Fungi</subject><subject>Glucosidase</subject><subject>Industrial Pollution Prevention</subject><subject>Lignocellulose</subject><subject>Monoculture</subject><subject>Operating costs</subject><subject>Original Paper</subject><subject>Pectinase</subject><subject>Pineapples</subject><subject>Refining</subject><subject>Renewable and Green Energy</subject><subject>Saccharification</subject><subject>Solid state</subject><subject>Sugar</subject><subject>Waste Management/Waste Technology</subject><subject>Wastes</subject><subject>Xylanase</subject><subject>β-Glucosidase</subject><issn>1877-2641</issn><issn>1877-265X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kctOBCEQRTtGE436A65IXLdS9AuWZnTURKPJ-NoRmqEVZaAFWjN-kN8pThvduapK5Zwqws2yPcAHgHFzGIDUJcsxwTkGYCyna9kW0KbJSV09rP_2JWxmuyE8Y4wJACVFs5V9XmqrF8KgE_uxXKiAJk6-RKENOlZvyrh-oWxE7RJNtRHfvRsCmg72USfSBuejFkhbNHNGz_NZFFGhyWCifhNRO4uEnaM7YZzXH-PAdehaWyX63iTSu3eL7kVIVrqxekPCJJoJKZ-E152WK20n2-iECWr3p25nt9OTm8lZfnF1ej45ushlUdUxh7rFCtcSKOsUKXHdCFl1ct5WtAJWSNJQqUBWJWNKMgwdbkGIOW2pLDGottjO9se9vXevgwqRP7vB23SSkwpIwaCqi0SRkZLeheBVx3ufPtEvOWD-HQkfI-EpEr6KhNMkFaMUEmwflf9b_Y_1BQeYkoI</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Teixeira, Wéllen Fabrícia Aviz</creator><creator>Batista, Ryhára Dias</creator><creator>do Amaral Santos, Claudia Cristina Auler</creator><creator>Júnior, Aloísio Chagas Freitas</creator><creator>Terrasan, César Rafael Fanchini</creator><creator>de Santana, Marcio Waluce Pinheiro R.</creator><creator>de Siqueira, Félix Gonçalves</creator><creator>de Paula-Elias, Fabrício Coutinho</creator><creator>de Almeida, Alex Fernando</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-2397-3318</orcidid><orcidid>https://orcid.org/0000-0002-7489-8701</orcidid><orcidid>https://orcid.org/0000-0001-5512-6119</orcidid><orcidid>https://orcid.org/0000-0001-5239-4994</orcidid><orcidid>https://orcid.org/0000-0002-7988-4755</orcidid><orcidid>https://orcid.org/0000-0001-8095-2156</orcidid><orcidid>https://orcid.org/0000-0003-3753-7568</orcidid><orcidid>https://orcid.org/0000-0001-5391-4621</orcidid></search><sort><creationdate>20210501</creationdate><title>Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification</title><author>Teixeira, Wéllen Fabrícia Aviz ; Batista, Ryhára Dias ; do Amaral Santos, Claudia Cristina Auler ; Júnior, Aloísio Chagas Freitas ; Terrasan, César Rafael Fanchini ; de Santana, Marcio Waluce Pinheiro R. ; de Siqueira, Félix Gonçalves ; de Paula-Elias, Fabrício Coutinho ; de Almeida, Alex Fernando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-16b0e06c189fe24067ac5fcdb585193c278ce1c5499ec901f0b1aad8b8c401eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agricultural wastes</topic><topic>Biorefineries</topic><topic>Cellobiase</topic><topic>Cellulose</topic><topic>Consortia</topic><topic>Cultivation</topic><topic>Endoglucanase</topic><topic>Engineering</topic><topic>Environment</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Enzymes</topic><topic>Fruits</topic><topic>Fungi</topic><topic>Glucosidase</topic><topic>Industrial Pollution Prevention</topic><topic>Lignocellulose</topic><topic>Monoculture</topic><topic>Operating costs</topic><topic>Original Paper</topic><topic>Pectinase</topic><topic>Pineapples</topic><topic>Refining</topic><topic>Renewable and Green Energy</topic><topic>Saccharification</topic><topic>Solid state</topic><topic>Sugar</topic><topic>Waste Management/Waste Technology</topic><topic>Wastes</topic><topic>Xylanase</topic><topic>β-Glucosidase</topic><toplevel>online_resources</toplevel><creatorcontrib>Teixeira, Wéllen Fabrícia Aviz</creatorcontrib><creatorcontrib>Batista, Ryhára Dias</creatorcontrib><creatorcontrib>do Amaral Santos, Claudia Cristina Auler</creatorcontrib><creatorcontrib>Júnior, Aloísio Chagas Freitas</creatorcontrib><creatorcontrib>Terrasan, César Rafael Fanchini</creatorcontrib><creatorcontrib>de Santana, Marcio Waluce Pinheiro R.</creatorcontrib><creatorcontrib>de Siqueira, Félix Gonçalves</creatorcontrib><creatorcontrib>de Paula-Elias, Fabrício Coutinho</creatorcontrib><creatorcontrib>de Almeida, Alex Fernando</creatorcontrib><collection>CrossRef</collection><jtitle>Waste and biomass valorization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teixeira, Wéllen Fabrícia Aviz</au><au>Batista, Ryhára Dias</au><au>do Amaral Santos, Claudia Cristina Auler</au><au>Júnior, Aloísio Chagas Freitas</au><au>Terrasan, César Rafael Fanchini</au><au>de Santana, Marcio Waluce Pinheiro R.</au><au>de Siqueira, Félix Gonçalves</au><au>de Paula-Elias, Fabrício Coutinho</au><au>de Almeida, Alex Fernando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification</atitle><jtitle>Waste and biomass valorization</jtitle><stitle>Waste Biomass Valor</stitle><date>2021-05-01</date><risdate>2021</risdate><volume>12</volume><issue>5</issue><spage>2521</spage><epage>2539</epage><pages>2521-2539</pages><issn>1877-2641</issn><eissn>1877-265X</eissn><abstract>Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of
Trichoderma
strains with
Aspergillus niger
or
Pleurotus ostreatus
were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of
Trichoderma asperellum
PEC-6 and
P. ostreatus
increased 1.60-fold the xylanase and 1.42-fold the β-glucosidase productions.
T. asperellum
PEC-17 and
A. niger
increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production.
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| fulltext | fulltext |
| identifier | ISSN: 1877-2641 |
| ispartof | Waste and biomass valorization, 2021-05, Vol.12 (5), p.2521-2539 |
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| subjects | Agricultural wastes Biorefineries Cellobiase Cellulose Consortia Cultivation Endoglucanase Engineering Environment Environmental Engineering/Biotechnology Enzymes Fruits Fungi Glucosidase Industrial Pollution Prevention Lignocellulose Monoculture Operating costs Original Paper Pectinase Pineapples Refining Renewable and Green Energy Saccharification Solid state Sugar Waste Management/Waste Technology Wastes Xylanase β-Glucosidase |
| title | Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification |
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