Antifungal activity of marine‐derived actinomycetes against Talaromyces marneffei
Aims This study aimed to isolate actinomycetes from marine environments and examine their antifungal activity against Talaromyces marneffei both in vitro and in vivo. Methods and Results Nineteen out of 101 actinomycete extracts were active and further determined for their minimum inhibitory concent...
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| Veröffentlicht in: | Journal of applied microbiology 2021-05, Vol.130 (5), p.1508-1522 |
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| creator | Sangkanu, S. Rukachaisirikul, V. Suriyachadkun, C. Phongpaichit, S. |
| description | Aims
This study aimed to isolate actinomycetes from marine environments and examine their antifungal activity against Talaromyces marneffei both in vitro and in vivo.
Methods and Results
Nineteen out of 101 actinomycete extracts were active and further determined for their minimum inhibitory concentrations (MIC). Three extracts of AMA50 that isolated from sediment showed strong antifungal activity against T. marneffei yeast (MICs ≤0·03–0·25 µg ml−1) and mould (MICs 0·5–16 µg ml−1) forms. The hexane extract from the cells of AMA50 (AMA50CH) exhibited the best activity against both the forms (MIC ≤ 1 µg ml−1). Three extracts from AMA50 killed the melanized yeast cells at 0·5 µg ml−1. The AMA50CH was further tested for protective effects in Caenorhabditis elegans model. At concentrations of 1–8 µg ml−1, the AMA50CH prolonged survival of T. marneffei‐infected C. elegans with a 60–70% survival rate. The composition of AMA50CH was determined by gas chromatography‐mass spectrometry. The major components were n‐hexadecanoic acid, tetradecanoic acid and pentadecanoic acid. Sequencing analysis revealed that isolate AMA50 belonged to the genus Streptomyces.
Conclusions
The AMA50CH from Streptomyces sp. AMA50 was the most effective extract against T. marneffei.
Significance and Impact of the Study
Talaromyces marneffei is one of the most important thermally dimorphic pathogenic fungi. These results indicated the potency of marine‐derived actinomycete extracts against T. marneffei both in vitro and in vivo. |
| doi_str_mv | 10.1111/jam.14877 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2511101852</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2511101852</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2687-2ff6d6e4ce7393a6ccafeff44a60e4c4388363bfb6618adfa7cae6594290c6783</originalsourceid><addsrcrecordid>eNp1kLtOwzAUhi0EoqUw8AIoEhNDWl8SOxmriquKGCiz5Th25SqXYidF2XgEnpEnwU0KG16Odc53flsfAJcITpE_s40opyhKGDsCY0RoHGLK8HF_j8IYMjwCZ85tIEQExvQUjAiBCMKUjsHrvGqMbqu1KAIhG7MzTRfUOiiFNZX6_vzKlTU7lffDqi47qRrlArEWpnJNsBKFsH3X7VcqpbUy5-BEi8Kpi0OdgLe729XiIVy-3D8u5stQYpqwEGtNc6oiqRhJiaBSCu33o0hQ6LsRSRJCSaYzSlEici2YFIrGaYRTKClLyARcD7lbW7-3yjV8U7e28k9yHHsvECUx9tTNQElbO2eV5ltr_F87jiDf6-NeH-_1efbqkNhmpcr_yF9fHpgNwIcpVPd_En-aPw-RP2Sre50</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2511101852</pqid></control><display><type>article</type><title>Antifungal activity of marine‐derived actinomycetes against Talaromyces marneffei</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Sangkanu, S. ; Rukachaisirikul, V. ; Suriyachadkun, C. ; Phongpaichit, S.</creator><creatorcontrib>Sangkanu, S. ; Rukachaisirikul, V. ; Suriyachadkun, C. ; Phongpaichit, S.</creatorcontrib><description>Aims
This study aimed to isolate actinomycetes from marine environments and examine their antifungal activity against Talaromyces marneffei both in vitro and in vivo.
Methods and Results
Nineteen out of 101 actinomycete extracts were active and further determined for their minimum inhibitory concentrations (MIC). Three extracts of AMA50 that isolated from sediment showed strong antifungal activity against T. marneffei yeast (MICs ≤0·03–0·25 µg ml−1) and mould (MICs 0·5–16 µg ml−1) forms. The hexane extract from the cells of AMA50 (AMA50CH) exhibited the best activity against both the forms (MIC ≤ 1 µg ml−1). Three extracts from AMA50 killed the melanized yeast cells at 0·5 µg ml−1. The AMA50CH was further tested for protective effects in Caenorhabditis elegans model. At concentrations of 1–8 µg ml−1, the AMA50CH prolonged survival of T. marneffei‐infected C. elegans with a 60–70% survival rate. The composition of AMA50CH was determined by gas chromatography‐mass spectrometry. The major components were n‐hexadecanoic acid, tetradecanoic acid and pentadecanoic acid. Sequencing analysis revealed that isolate AMA50 belonged to the genus Streptomyces.
Conclusions
The AMA50CH from Streptomyces sp. AMA50 was the most effective extract against T. marneffei.
Significance and Impact of the Study
Talaromyces marneffei is one of the most important thermally dimorphic pathogenic fungi. These results indicated the potency of marine‐derived actinomycete extracts against T. marneffei both in vitro and in vivo.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14877</identifier><identifier>PMID: 33010096</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Actinobacteria - chemistry ; Actinobacteria - isolation & purification ; Actinobacteria - physiology ; Actinomycetes ; Animals ; Antibiosis ; Antifungal activity ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; Antifungal Agents - toxicity ; Aquatic Organisms - microbiology ; Caenorhabditis elegans ; Caenorhabditis elegans - drug effects ; Caenorhabditis elegans - microbiology ; Fungicides ; Gas chromatography ; Geologic Sediments - microbiology ; Hexanes ; In vitro methods and tests ; In vivo methods and tests ; Marine environment ; marine‐derived actinomycetes ; Mass spectrometry ; Mass spectroscopy ; Microbial Sensitivity Tests ; Minimum inhibitory concentration ; Palmitic acid ; Sequence analysis ; Streptomyces ; Survival ; Talaromyces ; Talaromyces - drug effects ; Talaromyces - physiology ; Talaromyces - ultrastructure ; Talaromyces marneffei ; Yeast ; Yeasts</subject><ispartof>Journal of applied microbiology, 2021-05, Vol.130 (5), p.1508-1522</ispartof><rights>2020 The Society for Applied Microbiology</rights><rights>2020 The Society for Applied Microbiology.</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2687-2ff6d6e4ce7393a6ccafeff44a60e4c4388363bfb6618adfa7cae6594290c6783</citedby><cites>FETCH-LOGICAL-c2687-2ff6d6e4ce7393a6ccafeff44a60e4c4388363bfb6618adfa7cae6594290c6783</cites><orcidid>0000-0001-9924-6075</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14877$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14877$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33010096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sangkanu, S.</creatorcontrib><creatorcontrib>Rukachaisirikul, V.</creatorcontrib><creatorcontrib>Suriyachadkun, C.</creatorcontrib><creatorcontrib>Phongpaichit, S.</creatorcontrib><title>Antifungal activity of marine‐derived actinomycetes against Talaromyces marneffei</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
This study aimed to isolate actinomycetes from marine environments and examine their antifungal activity against Talaromyces marneffei both in vitro and in vivo.
Methods and Results
Nineteen out of 101 actinomycete extracts were active and further determined for their minimum inhibitory concentrations (MIC). Three extracts of AMA50 that isolated from sediment showed strong antifungal activity against T. marneffei yeast (MICs ≤0·03–0·25 µg ml−1) and mould (MICs 0·5–16 µg ml−1) forms. The hexane extract from the cells of AMA50 (AMA50CH) exhibited the best activity against both the forms (MIC ≤ 1 µg ml−1). Three extracts from AMA50 killed the melanized yeast cells at 0·5 µg ml−1. The AMA50CH was further tested for protective effects in Caenorhabditis elegans model. At concentrations of 1–8 µg ml−1, the AMA50CH prolonged survival of T. marneffei‐infected C. elegans with a 60–70% survival rate. The composition of AMA50CH was determined by gas chromatography‐mass spectrometry. The major components were n‐hexadecanoic acid, tetradecanoic acid and pentadecanoic acid. Sequencing analysis revealed that isolate AMA50 belonged to the genus Streptomyces.
Conclusions
The AMA50CH from Streptomyces sp. AMA50 was the most effective extract against T. marneffei.
Significance and Impact of the Study
Talaromyces marneffei is one of the most important thermally dimorphic pathogenic fungi. These results indicated the potency of marine‐derived actinomycete extracts against T. marneffei both in vitro and in vivo.</description><subject>Actinobacteria - chemistry</subject><subject>Actinobacteria - isolation & purification</subject><subject>Actinobacteria - physiology</subject><subject>Actinomycetes</subject><subject>Animals</subject><subject>Antibiosis</subject><subject>Antifungal activity</subject><subject>Antifungal Agents - isolation & purification</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antifungal Agents - toxicity</subject><subject>Aquatic Organisms - microbiology</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - drug effects</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>Fungicides</subject><subject>Gas chromatography</subject><subject>Geologic Sediments - microbiology</subject><subject>Hexanes</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Marine environment</subject><subject>marine‐derived actinomycetes</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microbial Sensitivity Tests</subject><subject>Minimum inhibitory concentration</subject><subject>Palmitic acid</subject><subject>Sequence analysis</subject><subject>Streptomyces</subject><subject>Survival</subject><subject>Talaromyces</subject><subject>Talaromyces - drug effects</subject><subject>Talaromyces - physiology</subject><subject>Talaromyces - ultrastructure</subject><subject>Talaromyces marneffei</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtOwzAUhi0EoqUw8AIoEhNDWl8SOxmriquKGCiz5Th25SqXYidF2XgEnpEnwU0KG16Odc53flsfAJcITpE_s40opyhKGDsCY0RoHGLK8HF_j8IYMjwCZ85tIEQExvQUjAiBCMKUjsHrvGqMbqu1KAIhG7MzTRfUOiiFNZX6_vzKlTU7lffDqi47qRrlArEWpnJNsBKFsH3X7VcqpbUy5-BEi8Kpi0OdgLe729XiIVy-3D8u5stQYpqwEGtNc6oiqRhJiaBSCu33o0hQ6LsRSRJCSaYzSlEici2YFIrGaYRTKClLyARcD7lbW7-3yjV8U7e28k9yHHsvECUx9tTNQElbO2eV5ltr_F87jiDf6-NeH-_1efbqkNhmpcr_yF9fHpgNwIcpVPd_En-aPw-RP2Sre50</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Sangkanu, S.</creator><creator>Rukachaisirikul, V.</creator><creator>Suriyachadkun, C.</creator><creator>Phongpaichit, S.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-9924-6075</orcidid></search><sort><creationdate>202105</creationdate><title>Antifungal activity of marine‐derived actinomycetes against Talaromyces marneffei</title><author>Sangkanu, S. ; Rukachaisirikul, V. ; Suriyachadkun, C. ; Phongpaichit, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2687-2ff6d6e4ce7393a6ccafeff44a60e4c4388363bfb6618adfa7cae6594290c6783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Actinobacteria - chemistry</topic><topic>Actinobacteria - isolation & purification</topic><topic>Actinobacteria - physiology</topic><topic>Actinomycetes</topic><topic>Animals</topic><topic>Antibiosis</topic><topic>Antifungal activity</topic><topic>Antifungal Agents - isolation & purification</topic><topic>Antifungal Agents - pharmacology</topic><topic>Antifungal Agents - toxicity</topic><topic>Aquatic Organisms - microbiology</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - drug effects</topic><topic>Caenorhabditis elegans - microbiology</topic><topic>Fungicides</topic><topic>Gas chromatography</topic><topic>Geologic Sediments - microbiology</topic><topic>Hexanes</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Marine environment</topic><topic>marine‐derived actinomycetes</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microbial Sensitivity Tests</topic><topic>Minimum inhibitory concentration</topic><topic>Palmitic acid</topic><topic>Sequence analysis</topic><topic>Streptomyces</topic><topic>Survival</topic><topic>Talaromyces</topic><topic>Talaromyces - drug effects</topic><topic>Talaromyces - physiology</topic><topic>Talaromyces - ultrastructure</topic><topic>Talaromyces marneffei</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sangkanu, S.</creatorcontrib><creatorcontrib>Rukachaisirikul, V.</creatorcontrib><creatorcontrib>Suriyachadkun, C.</creatorcontrib><creatorcontrib>Phongpaichit, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sangkanu, S.</au><au>Rukachaisirikul, V.</au><au>Suriyachadkun, C.</au><au>Phongpaichit, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antifungal activity of marine‐derived actinomycetes against Talaromyces marneffei</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2021-05</date><risdate>2021</risdate><volume>130</volume><issue>5</issue><spage>1508</spage><epage>1522</epage><pages>1508-1522</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
This study aimed to isolate actinomycetes from marine environments and examine their antifungal activity against Talaromyces marneffei both in vitro and in vivo.
Methods and Results
Nineteen out of 101 actinomycete extracts were active and further determined for their minimum inhibitory concentrations (MIC). Three extracts of AMA50 that isolated from sediment showed strong antifungal activity against T. marneffei yeast (MICs ≤0·03–0·25 µg ml−1) and mould (MICs 0·5–16 µg ml−1) forms. The hexane extract from the cells of AMA50 (AMA50CH) exhibited the best activity against both the forms (MIC ≤ 1 µg ml−1). Three extracts from AMA50 killed the melanized yeast cells at 0·5 µg ml−1. The AMA50CH was further tested for protective effects in Caenorhabditis elegans model. At concentrations of 1–8 µg ml−1, the AMA50CH prolonged survival of T. marneffei‐infected C. elegans with a 60–70% survival rate. The composition of AMA50CH was determined by gas chromatography‐mass spectrometry. The major components were n‐hexadecanoic acid, tetradecanoic acid and pentadecanoic acid. Sequencing analysis revealed that isolate AMA50 belonged to the genus Streptomyces.
Conclusions
The AMA50CH from Streptomyces sp. AMA50 was the most effective extract against T. marneffei.
Significance and Impact of the Study
Talaromyces marneffei is one of the most important thermally dimorphic pathogenic fungi. These results indicated the potency of marine‐derived actinomycete extracts against T. marneffei both in vitro and in vivo.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33010096</pmid><doi>10.1111/jam.14877</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-9924-6075</orcidid></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Actinobacteria - chemistry Actinobacteria - isolation & purification Actinobacteria - physiology Actinomycetes Animals Antibiosis Antifungal activity Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Antifungal Agents - toxicity Aquatic Organisms - microbiology Caenorhabditis elegans Caenorhabditis elegans - drug effects Caenorhabditis elegans - microbiology Fungicides Gas chromatography Geologic Sediments - microbiology Hexanes In vitro methods and tests In vivo methods and tests Marine environment marine‐derived actinomycetes Mass spectrometry Mass spectroscopy Microbial Sensitivity Tests Minimum inhibitory concentration Palmitic acid Sequence analysis Streptomyces Survival Talaromyces Talaromyces - drug effects Talaromyces - physiology Talaromyces - ultrastructure Talaromyces marneffei Yeast Yeasts |
| title | Antifungal activity of marine‐derived actinomycetes against Talaromyces marneffei |
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