Analysis of Amylase in the Kitchen: An At-Home Biochemistry Experiment for the COVID-19 Pandemic

During the COVID-19 pandemic, an at-home enzyme assay was developed for a biochemistry laboratory course at Arizona State University. The experiment was designed to use items that could be easily obtained and safe to use. The experiment focused on the analysis of salivary amylase using starch from f...

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Veröffentlicht in:Journal of chemical education 2021-03, Vol.98 (3), p.858-865
Hauptverfasser: Maqsood, Sehrish, Kilpatrick, Samuel M, Truong, Chloe D, Lefler, Scott R
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container_title Journal of chemical education
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creator Maqsood, Sehrish
Kilpatrick, Samuel M
Truong, Chloe D
Lefler, Scott R
description During the COVID-19 pandemic, an at-home enzyme assay was developed for a biochemistry laboratory course at Arizona State University. The experiment was designed to use items that could be easily obtained and safe to use. The experiment focused on the analysis of salivary amylase using starch from food as a substrate, black tea as an inhibitor, and tincture of iodine to quantify the amount of starch present. In this article, we describe the design of the experiment and an analysis of the student performance with specific mention of the common problems that the students experienced. While this experiment was specifically designed as an at-home experiment, it could easily be adapted to use in a typical undergraduate biochemistry laboratory.
doi_str_mv 10.1021/acs.jchemed.0c01236
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source American Chemical Society Journals
subjects Amylases
Biochemistry
Black tea
College Science
College students
Colleges & universities
COVID-19
Distance Education
Experiments
Food
Hands on Science
Iodine
Laboratory Experiments
Pandemics
Safety
Starch
Studies
Substrate inhibition
Undergraduate Students
title Analysis of Amylase in the Kitchen: An At-Home Biochemistry Experiment for the COVID-19 Pandemic
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