Genome‐wide identification of long non‐coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis
BACKGROUND Long non‐coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified in many species including insects. However, the association between lncRNAs and pesticide resistance in insect species such as Bactrocera dorsalis is unknown. RESULTS RNA...
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| Veröffentlicht in: | Pest management science 2021-05, Vol.77 (5), p.2292-2301 |
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| creator | Meng, Li‐Wei Yuan, Guo‐Rui Chen, Meng‐Ling Dou, Wei Jing, Tian‐Xing Zheng, Li‐Sha Peng, Meng‐Lan Bai, Wen‐Jie Wang, Jin‐Jun |
| description | BACKGROUND
Long non‐coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified in many species including insects. However, the association between lncRNAs and pesticide resistance in insect species such as Bactrocera dorsalis is unknown.
RESULTS
RNA‐seq was performed on malathion resistant (MR1) and susceptible (MS) strains of B. dorsalis and a total of 6171 lncRNAs transcripts were identified. These included 3728 lincRNAs, 653 antisense lncRNAs, 1402 intronic lncRNAs, and 388 sense lncRNAs. A total of 40 and 52 upregulated lncRNAs were found in females and males of the MR1 strain compared to 54 and 49 in the same sexes of the MS strain, respectively. Twenty‐seven of these lncRNAs showed the same trend of expression in both females and males in the MR1 strain, in which 15 lncRNAs were upregulated and 12 were downregulated. RT‐qPCR results indicated that the differentially expressed lncRNAs were associated with malathion resistance. The lnc15010.10 and lnc3774.2 were highly expressed in the cuticle of the MR1 strain, indicating that these two lncRNAs may be related to malathion resistance. RNAi of lnc3774.2 and a bioassay showed that malathion resistance was possibly influenced by changes in the B. dorsalis cuticle.
CONCLUSION
LncRNAs of B. dorsalis potentially related to the malathion resistance were identified. Two lncRNAs appear to influence malathion resistance via modulating the structure, or components, of the cuticle. © 2021 Society of Chemical Industry
(1) The 6,171 long non‐coding RNAs of Bactrocera dorsalis were first identified in genome‐wide. (2) lnc3774.2 and lnc15010.10 might participated in malathion resistance of B. dorsalis via modulate cuticle. |
| doi_str_mv | 10.1002/ps.6256 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2509526517</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2509526517</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3456-87b57d88e30eab1d74e9117bd7d41a3bac2b8160d4750f27598bcbd28c7763063</originalsourceid><addsrcrecordid>eNp1kM1KAzEQx4MoVqv4BhLwoCKt-dhsdo-1aBWKih_gbckmWZuyTWqypRQ8-Ag-o09iarU3D8PMwG9-A38ADjDqYoTI-TR0U8LSDbCDGUk7SZ5nm-s5e2mB3RDGCKE8z8k2aFGaEEpTtgPeB9q6if76-JwbpWEs25jKSNEYZ6GrYO3sK7TORkI6ZeLycNsL8KS2cjmcQhGCk0Y0WsG5aUZwImrRjJbXXgcTGmFl9Fp4IWTjndReQOV8ELUJe2CrEnXQ-7-9DZ6vLp_6153h3eCm3xt2JE1Y2sl4ybjKMk2RFiVWPNE5xrxUXCVY0FJIUmY4RSrhDFWEszwrZalIJjlPKUppGxytvFPv3mY6NMXYzbyNLwvCUB5TYphH6nhFSe9C8Loqpt5MhF8UGBXLlItpKJYpR_Lw1zcrJ1qtub9YI3C2Auam1ov_PMX944_uGwl7h7w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2509526517</pqid></control><display><type>article</type><title>Genome‐wide identification of long non‐coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Meng, Li‐Wei ; Yuan, Guo‐Rui ; Chen, Meng‐Ling ; Dou, Wei ; Jing, Tian‐Xing ; Zheng, Li‐Sha ; Peng, Meng‐Lan ; Bai, Wen‐Jie ; Wang, Jin‐Jun</creator><creatorcontrib>Meng, Li‐Wei ; Yuan, Guo‐Rui ; Chen, Meng‐Ling ; Dou, Wei ; Jing, Tian‐Xing ; Zheng, Li‐Sha ; Peng, Meng‐Lan ; Bai, Wen‐Jie ; Wang, Jin‐Jun</creatorcontrib><description>BACKGROUND
Long non‐coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified in many species including insects. However, the association between lncRNAs and pesticide resistance in insect species such as Bactrocera dorsalis is unknown.
RESULTS
RNA‐seq was performed on malathion resistant (MR1) and susceptible (MS) strains of B. dorsalis and a total of 6171 lncRNAs transcripts were identified. These included 3728 lincRNAs, 653 antisense lncRNAs, 1402 intronic lncRNAs, and 388 sense lncRNAs. A total of 40 and 52 upregulated lncRNAs were found in females and males of the MR1 strain compared to 54 and 49 in the same sexes of the MS strain, respectively. Twenty‐seven of these lncRNAs showed the same trend of expression in both females and males in the MR1 strain, in which 15 lncRNAs were upregulated and 12 were downregulated. RT‐qPCR results indicated that the differentially expressed lncRNAs were associated with malathion resistance. The lnc15010.10 and lnc3774.2 were highly expressed in the cuticle of the MR1 strain, indicating that these two lncRNAs may be related to malathion resistance. RNAi of lnc3774.2 and a bioassay showed that malathion resistance was possibly influenced by changes in the B. dorsalis cuticle.
CONCLUSION
LncRNAs of B. dorsalis potentially related to the malathion resistance were identified. Two lncRNAs appear to influence malathion resistance via modulating the structure, or components, of the cuticle. © 2021 Society of Chemical Industry
(1) The 6,171 long non‐coding RNAs of Bactrocera dorsalis were first identified in genome‐wide. (2) lnc3774.2 and lnc15010.10 might participated in malathion resistance of B. dorsalis via modulate cuticle.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.6256</identifier><identifier>PMID: 33423365</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Bactrocera dorsalis ; Bioassays ; Biological activity ; cuticle ; Epicuticle ; Female ; Females ; Genomes ; Insecticide resistance ; Insecticide Resistance - genetics ; Insecticides - pharmacology ; Insects ; long non‐coding RNA ; Malathion ; Malathion - pharmacology ; malathion resistance ; Male ; Males ; Non-coding RNA ; Pesticide resistance ; Pesticides ; RNA, Long Noncoding - genetics ; RNA-mediated interference ; RNA‐seq ; Tephritidae - genetics</subject><ispartof>Pest management science, 2021-05, Vol.77 (5), p.2292-2301</ispartof><rights>2021 Society of Chemical Industry</rights><rights>2021 Society of Chemical Industry.</rights><rights>Copyright © 2021 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3456-87b57d88e30eab1d74e9117bd7d41a3bac2b8160d4750f27598bcbd28c7763063</citedby><cites>FETCH-LOGICAL-c3456-87b57d88e30eab1d74e9117bd7d41a3bac2b8160d4750f27598bcbd28c7763063</cites><orcidid>0000-0002-9678-5425 ; 0000-0002-8777-5268</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.6256$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.6256$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33423365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meng, Li‐Wei</creatorcontrib><creatorcontrib>Yuan, Guo‐Rui</creatorcontrib><creatorcontrib>Chen, Meng‐Ling</creatorcontrib><creatorcontrib>Dou, Wei</creatorcontrib><creatorcontrib>Jing, Tian‐Xing</creatorcontrib><creatorcontrib>Zheng, Li‐Sha</creatorcontrib><creatorcontrib>Peng, Meng‐Lan</creatorcontrib><creatorcontrib>Bai, Wen‐Jie</creatorcontrib><creatorcontrib>Wang, Jin‐Jun</creatorcontrib><title>Genome‐wide identification of long non‐coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Long non‐coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified in many species including insects. However, the association between lncRNAs and pesticide resistance in insect species such as Bactrocera dorsalis is unknown.
RESULTS
RNA‐seq was performed on malathion resistant (MR1) and susceptible (MS) strains of B. dorsalis and a total of 6171 lncRNAs transcripts were identified. These included 3728 lincRNAs, 653 antisense lncRNAs, 1402 intronic lncRNAs, and 388 sense lncRNAs. A total of 40 and 52 upregulated lncRNAs were found in females and males of the MR1 strain compared to 54 and 49 in the same sexes of the MS strain, respectively. Twenty‐seven of these lncRNAs showed the same trend of expression in both females and males in the MR1 strain, in which 15 lncRNAs were upregulated and 12 were downregulated. RT‐qPCR results indicated that the differentially expressed lncRNAs were associated with malathion resistance. The lnc15010.10 and lnc3774.2 were highly expressed in the cuticle of the MR1 strain, indicating that these two lncRNAs may be related to malathion resistance. RNAi of lnc3774.2 and a bioassay showed that malathion resistance was possibly influenced by changes in the B. dorsalis cuticle.
CONCLUSION
LncRNAs of B. dorsalis potentially related to the malathion resistance were identified. Two lncRNAs appear to influence malathion resistance via modulating the structure, or components, of the cuticle. © 2021 Society of Chemical Industry
(1) The 6,171 long non‐coding RNAs of Bactrocera dorsalis were first identified in genome‐wide. (2) lnc3774.2 and lnc15010.10 might participated in malathion resistance of B. dorsalis via modulate cuticle.</description><subject>Animals</subject><subject>Bactrocera dorsalis</subject><subject>Bioassays</subject><subject>Biological activity</subject><subject>cuticle</subject><subject>Epicuticle</subject><subject>Female</subject><subject>Females</subject><subject>Genomes</subject><subject>Insecticide resistance</subject><subject>Insecticide Resistance - genetics</subject><subject>Insecticides - pharmacology</subject><subject>Insects</subject><subject>long non‐coding RNA</subject><subject>Malathion</subject><subject>Malathion - pharmacology</subject><subject>malathion resistance</subject><subject>Male</subject><subject>Males</subject><subject>Non-coding RNA</subject><subject>Pesticide resistance</subject><subject>Pesticides</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA-mediated interference</subject><subject>RNA‐seq</subject><subject>Tephritidae - genetics</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1KAzEQx4MoVqv4BhLwoCKt-dhsdo-1aBWKih_gbckmWZuyTWqypRQ8-Ag-o09iarU3D8PMwG9-A38ADjDqYoTI-TR0U8LSDbCDGUk7SZ5nm-s5e2mB3RDGCKE8z8k2aFGaEEpTtgPeB9q6if76-JwbpWEs25jKSNEYZ6GrYO3sK7TORkI6ZeLycNsL8KS2cjmcQhGCk0Y0WsG5aUZwImrRjJbXXgcTGmFl9Fp4IWTjndReQOV8ELUJe2CrEnXQ-7-9DZ6vLp_6153h3eCm3xt2JE1Y2sl4ybjKMk2RFiVWPNE5xrxUXCVY0FJIUmY4RSrhDFWEszwrZalIJjlPKUppGxytvFPv3mY6NMXYzbyNLwvCUB5TYphH6nhFSe9C8Loqpt5MhF8UGBXLlItpKJYpR_Lw1zcrJ1qtub9YI3C2Auam1ov_PMX944_uGwl7h7w</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Meng, Li‐Wei</creator><creator>Yuan, Guo‐Rui</creator><creator>Chen, Meng‐Ling</creator><creator>Dou, Wei</creator><creator>Jing, Tian‐Xing</creator><creator>Zheng, Li‐Sha</creator><creator>Peng, Meng‐Lan</creator><creator>Bai, Wen‐Jie</creator><creator>Wang, Jin‐Jun</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-9678-5425</orcidid><orcidid>https://orcid.org/0000-0002-8777-5268</orcidid></search><sort><creationdate>202105</creationdate><title>Genome‐wide identification of long non‐coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis</title><author>Meng, Li‐Wei ; Yuan, Guo‐Rui ; Chen, Meng‐Ling ; Dou, Wei ; Jing, Tian‐Xing ; Zheng, Li‐Sha ; Peng, Meng‐Lan ; Bai, Wen‐Jie ; Wang, Jin‐Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3456-87b57d88e30eab1d74e9117bd7d41a3bac2b8160d4750f27598bcbd28c7763063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Bactrocera dorsalis</topic><topic>Bioassays</topic><topic>Biological activity</topic><topic>cuticle</topic><topic>Epicuticle</topic><topic>Female</topic><topic>Females</topic><topic>Genomes</topic><topic>Insecticide resistance</topic><topic>Insecticide Resistance - genetics</topic><topic>Insecticides - pharmacology</topic><topic>Insects</topic><topic>long non‐coding RNA</topic><topic>Malathion</topic><topic>Malathion - pharmacology</topic><topic>malathion resistance</topic><topic>Male</topic><topic>Males</topic><topic>Non-coding RNA</topic><topic>Pesticide resistance</topic><topic>Pesticides</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA-mediated interference</topic><topic>RNA‐seq</topic><topic>Tephritidae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meng, Li‐Wei</creatorcontrib><creatorcontrib>Yuan, Guo‐Rui</creatorcontrib><creatorcontrib>Chen, Meng‐Ling</creatorcontrib><creatorcontrib>Dou, Wei</creatorcontrib><creatorcontrib>Jing, Tian‐Xing</creatorcontrib><creatorcontrib>Zheng, Li‐Sha</creatorcontrib><creatorcontrib>Peng, Meng‐Lan</creatorcontrib><creatorcontrib>Bai, Wen‐Jie</creatorcontrib><creatorcontrib>Wang, Jin‐Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meng, Li‐Wei</au><au>Yuan, Guo‐Rui</au><au>Chen, Meng‐Ling</au><au>Dou, Wei</au><au>Jing, Tian‐Xing</au><au>Zheng, Li‐Sha</au><au>Peng, Meng‐Lan</au><au>Bai, Wen‐Jie</au><au>Wang, Jin‐Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome‐wide identification of long non‐coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2021-05</date><risdate>2021</risdate><volume>77</volume><issue>5</issue><spage>2292</spage><epage>2301</epage><pages>2292-2301</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Long non‐coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified in many species including insects. However, the association between lncRNAs and pesticide resistance in insect species such as Bactrocera dorsalis is unknown.
RESULTS
RNA‐seq was performed on malathion resistant (MR1) and susceptible (MS) strains of B. dorsalis and a total of 6171 lncRNAs transcripts were identified. These included 3728 lincRNAs, 653 antisense lncRNAs, 1402 intronic lncRNAs, and 388 sense lncRNAs. A total of 40 and 52 upregulated lncRNAs were found in females and males of the MR1 strain compared to 54 and 49 in the same sexes of the MS strain, respectively. Twenty‐seven of these lncRNAs showed the same trend of expression in both females and males in the MR1 strain, in which 15 lncRNAs were upregulated and 12 were downregulated. RT‐qPCR results indicated that the differentially expressed lncRNAs were associated with malathion resistance. The lnc15010.10 and lnc3774.2 were highly expressed in the cuticle of the MR1 strain, indicating that these two lncRNAs may be related to malathion resistance. RNAi of lnc3774.2 and a bioassay showed that malathion resistance was possibly influenced by changes in the B. dorsalis cuticle.
CONCLUSION
LncRNAs of B. dorsalis potentially related to the malathion resistance were identified. Two lncRNAs appear to influence malathion resistance via modulating the structure, or components, of the cuticle. © 2021 Society of Chemical Industry
(1) The 6,171 long non‐coding RNAs of Bactrocera dorsalis were first identified in genome‐wide. (2) lnc3774.2 and lnc15010.10 might participated in malathion resistance of B. dorsalis via modulate cuticle.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>33423365</pmid><doi>10.1002/ps.6256</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9678-5425</orcidid><orcidid>https://orcid.org/0000-0002-8777-5268</orcidid></addata></record> |
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| subjects | Animals Bactrocera dorsalis Bioassays Biological activity cuticle Epicuticle Female Females Genomes Insecticide resistance Insecticide Resistance - genetics Insecticides - pharmacology Insects long non‐coding RNA Malathion Malathion - pharmacology malathion resistance Male Males Non-coding RNA Pesticide resistance Pesticides RNA, Long Noncoding - genetics RNA-mediated interference RNA‐seq Tephritidae - genetics |
| title | Genome‐wide identification of long non‐coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis |
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