Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)
Channel catfish ( Ictalurus punctatus ) is the primary culture species in the US along with its hybrid made with male blue catfish, I. furcatus . In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin prom...
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| creator | Huang, Yingqi Bugg, William Bangs, Max Qin, Guyu Drescher, David Backenstose, Nathan Weng, Chia Chen Zhang, Yiliu Khalil, Karim Dong, Sheng Elaswad, Ahmed Ye, Zhi Lu, Cuiyu Vo, Khoi Simora, Rhoda Mae Ma, Xiaoli Taylor, Zachary Yang, Yujia Zhou, Tao Guo, Jingping Salze, Guillaume Qin, Zhenkui Wang, Yi Dunham, Rex A. |
| description | Channel catfish (
Ictalurus punctatus
) is the primary culture species in the US along with its hybrid made with male blue catfish,
I. furcatus
. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (
P
|
| doi_str_mv | 10.1007/s11248-021-00242-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2509430026</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2509430026</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-778d2f95d769327bc724e65760aae8e0cbd2b51a28102c9c58378b8f0c74c4453</originalsourceid><addsrcrecordid>eNp9kc1uFSEYhonR2GP1BlwYEjd1QeVnGGBpelptUuNCXROG-aZnmjkw8rMw8WK8Fq-s1FM16cIVBJ73_QgPQi8ZPWWUqreZMd5pQjkjlPKOE_YIbZhUghjR68doQ03PidbMHKFnOd9Q2mJaPEVHQijDlZEb9GM7J_AFuzDidYE5lhTX2WOYpnaccZxw2QH-6HKs-LNb9jHgLSzFEfnr5xayKzW5DLgkF_I1BMBzwBcM-50LARbsXZnmvMMnl764paaa8VpD25ea3zxHTya3ZHhxvx6jrxfnX84-kKtP7y_P3l0RL5QsRCk98snIUfVGcDV4xTvopeqpc6CB-mHkg2SOa0a5N15qofSgJ-pV57tOimN0cuhdU_xWIRe7n7OHZXEBYs2WS6qUELzXDX39AL2JNYX2ujvKdKL9dN8ofqB8ijknmOya5r1L3y2j9s6NPbixzY397cayFnp1X12HPYx_I39kNEAcgNyuwjWkf7P_U3sLDj6Zxw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2509430026</pqid></control><display><type>article</type><title>Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Huang, Yingqi ; Bugg, William ; Bangs, Max ; Qin, Guyu ; Drescher, David ; Backenstose, Nathan ; Weng, Chia Chen ; Zhang, Yiliu ; Khalil, Karim ; Dong, Sheng ; Elaswad, Ahmed ; Ye, Zhi ; Lu, Cuiyu ; Vo, Khoi ; Simora, Rhoda Mae ; Ma, Xiaoli ; Taylor, Zachary ; Yang, Yujia ; Zhou, Tao ; Guo, Jingping ; Salze, Guillaume ; Qin, Zhenkui ; Wang, Yi ; Dunham, Rex A.</creator><creatorcontrib>Huang, Yingqi ; Bugg, William ; Bangs, Max ; Qin, Guyu ; Drescher, David ; Backenstose, Nathan ; Weng, Chia Chen ; Zhang, Yiliu ; Khalil, Karim ; Dong, Sheng ; Elaswad, Ahmed ; Ye, Zhi ; Lu, Cuiyu ; Vo, Khoi ; Simora, Rhoda Mae ; Ma, Xiaoli ; Taylor, Zachary ; Yang, Yujia ; Zhou, Tao ; Guo, Jingping ; Salze, Guillaume ; Qin, Zhenkui ; Wang, Yi ; Dunham, Rex A.</creatorcontrib><description>Channel catfish (
Ictalurus punctatus
) is the primary culture species in the US along with its hybrid made with male blue catfish,
I. furcatus
. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (
P
< 0.01,
P
< 0.01,
P
< 0.01). However, while the relative proportion of n-3 fatty acids was achieved, the total amount of fatty acids in the transgenic fish decreased resulting in a reduction of all fatty acids. Insertion of the βactin-D5D transgene into channel catfish also had large effects on the body composition, and growth of channel catfish. Transgenic channel catfish grew faster, were more disease resistant, had higher protein and moisture percentage, but lower fat percentage than full-sib controls. There were sex effects as performance changes were more dramatic and significant in males. The βactin-D5D transgenic channel catfish were also more uniform in their fatty acid composition, growth and other traits.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-021-00242-1</identifier><identifier>PMID: 33792795</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Acid production ; Actin ; Animal Genetics and Genomics ; Animals ; Animals, Genetically Modified - growth & development ; Animals, Genetically Modified - immunology ; Animals, Genetically Modified - metabolism ; Animals, Genetically Modified - microbiology ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Body composition ; Catfish ; Delta-5 Fatty Acid Desaturase - genetics ; Delta-5 Fatty Acid Desaturase - metabolism ; Desaturase ; Disease resistance ; Fatty acid composition ; Fatty acids ; Fatty Acids - metabolism ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Flavobacterium - physiology ; Genetic Engineering ; Ictaluridae - growth & development ; Ictaluridae - immunology ; Ictaluridae - metabolism ; Ictaluridae - microbiology ; Ictalurus punctatus ; Life Sciences ; Molecular Medicine ; Nutritive value ; Oncorhynchus masou ; Original Paper ; Plant Genetics and Genomics ; Transgenes ; Transgenic fish ; Transgenics</subject><ispartof>Transgenic research, 2021-04, Vol.30 (2), p.185-200</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-778d2f95d769327bc724e65760aae8e0cbd2b51a28102c9c58378b8f0c74c4453</citedby><cites>FETCH-LOGICAL-c375t-778d2f95d769327bc724e65760aae8e0cbd2b51a28102c9c58378b8f0c74c4453</cites><orcidid>0000-0002-6149-6017</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-021-00242-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-021-00242-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33792795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yingqi</creatorcontrib><creatorcontrib>Bugg, William</creatorcontrib><creatorcontrib>Bangs, Max</creatorcontrib><creatorcontrib>Qin, Guyu</creatorcontrib><creatorcontrib>Drescher, David</creatorcontrib><creatorcontrib>Backenstose, Nathan</creatorcontrib><creatorcontrib>Weng, Chia Chen</creatorcontrib><creatorcontrib>Zhang, Yiliu</creatorcontrib><creatorcontrib>Khalil, Karim</creatorcontrib><creatorcontrib>Dong, Sheng</creatorcontrib><creatorcontrib>Elaswad, Ahmed</creatorcontrib><creatorcontrib>Ye, Zhi</creatorcontrib><creatorcontrib>Lu, Cuiyu</creatorcontrib><creatorcontrib>Vo, Khoi</creatorcontrib><creatorcontrib>Simora, Rhoda Mae</creatorcontrib><creatorcontrib>Ma, Xiaoli</creatorcontrib><creatorcontrib>Taylor, Zachary</creatorcontrib><creatorcontrib>Yang, Yujia</creatorcontrib><creatorcontrib>Zhou, Tao</creatorcontrib><creatorcontrib>Guo, Jingping</creatorcontrib><creatorcontrib>Salze, Guillaume</creatorcontrib><creatorcontrib>Qin, Zhenkui</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Dunham, Rex A.</creatorcontrib><title>Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Channel catfish (
Ictalurus punctatus
) is the primary culture species in the US along with its hybrid made with male blue catfish,
I. furcatus
. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (
P
< 0.01,
P
< 0.01,
P
< 0.01). However, while the relative proportion of n-3 fatty acids was achieved, the total amount of fatty acids in the transgenic fish decreased resulting in a reduction of all fatty acids. Insertion of the βactin-D5D transgene into channel catfish also had large effects on the body composition, and growth of channel catfish. Transgenic channel catfish grew faster, were more disease resistant, had higher protein and moisture percentage, but lower fat percentage than full-sib controls. There were sex effects as performance changes were more dramatic and significant in males. The βactin-D5D transgenic channel catfish were also more uniform in their fatty acid composition, growth and other traits.</description><subject>Acid production</subject><subject>Actin</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Animals, Genetically Modified - growth & development</subject><subject>Animals, Genetically Modified - immunology</subject><subject>Animals, Genetically Modified - metabolism</subject><subject>Animals, Genetically Modified - microbiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Body composition</subject><subject>Catfish</subject><subject>Delta-5 Fatty Acid Desaturase - genetics</subject><subject>Delta-5 Fatty Acid Desaturase - metabolism</subject><subject>Desaturase</subject><subject>Disease resistance</subject><subject>Fatty acid composition</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Flavobacterium - physiology</subject><subject>Genetic Engineering</subject><subject>Ictaluridae - growth & development</subject><subject>Ictaluridae - immunology</subject><subject>Ictaluridae - metabolism</subject><subject>Ictaluridae - microbiology</subject><subject>Ictalurus punctatus</subject><subject>Life Sciences</subject><subject>Molecular Medicine</subject><subject>Nutritive value</subject><subject>Oncorhynchus masou</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Transgenes</subject><subject>Transgenic fish</subject><subject>Transgenics</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1uFSEYhonR2GP1BlwYEjd1QeVnGGBpelptUuNCXROG-aZnmjkw8rMw8WK8Fq-s1FM16cIVBJ73_QgPQi8ZPWWUqreZMd5pQjkjlPKOE_YIbZhUghjR68doQ03PidbMHKFnOd9Q2mJaPEVHQijDlZEb9GM7J_AFuzDidYE5lhTX2WOYpnaccZxw2QH-6HKs-LNb9jHgLSzFEfnr5xayKzW5DLgkF_I1BMBzwBcM-50LARbsXZnmvMMnl764paaa8VpD25ea3zxHTya3ZHhxvx6jrxfnX84-kKtP7y_P3l0RL5QsRCk98snIUfVGcDV4xTvopeqpc6CB-mHkg2SOa0a5N15qofSgJ-pV57tOimN0cuhdU_xWIRe7n7OHZXEBYs2WS6qUELzXDX39AL2JNYX2ujvKdKL9dN8ofqB8ijknmOya5r1L3y2j9s6NPbixzY397cayFnp1X12HPYx_I39kNEAcgNyuwjWkf7P_U3sLDj6Zxw</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Huang, Yingqi</creator><creator>Bugg, William</creator><creator>Bangs, Max</creator><creator>Qin, Guyu</creator><creator>Drescher, David</creator><creator>Backenstose, Nathan</creator><creator>Weng, Chia Chen</creator><creator>Zhang, Yiliu</creator><creator>Khalil, Karim</creator><creator>Dong, Sheng</creator><creator>Elaswad, Ahmed</creator><creator>Ye, Zhi</creator><creator>Lu, Cuiyu</creator><creator>Vo, Khoi</creator><creator>Simora, Rhoda Mae</creator><creator>Ma, Xiaoli</creator><creator>Taylor, Zachary</creator><creator>Yang, Yujia</creator><creator>Zhou, Tao</creator><creator>Guo, Jingping</creator><creator>Salze, Guillaume</creator><creator>Qin, Zhenkui</creator><creator>Wang, Yi</creator><creator>Dunham, Rex A.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6149-6017</orcidid></search><sort><creationdate>20210401</creationdate><title>Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)</title><author>Huang, Yingqi ; Bugg, William ; Bangs, Max ; Qin, Guyu ; Drescher, David ; Backenstose, Nathan ; Weng, Chia Chen ; Zhang, Yiliu ; Khalil, Karim ; Dong, Sheng ; Elaswad, Ahmed ; Ye, Zhi ; Lu, Cuiyu ; Vo, Khoi ; Simora, Rhoda Mae ; Ma, Xiaoli ; Taylor, Zachary ; Yang, Yujia ; Zhou, Tao ; Guo, Jingping ; Salze, Guillaume ; Qin, Zhenkui ; Wang, Yi ; Dunham, Rex A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-778d2f95d769327bc724e65760aae8e0cbd2b51a28102c9c58378b8f0c74c4453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acid production</topic><topic>Actin</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Animals, Genetically Modified - growth & development</topic><topic>Animals, Genetically Modified - immunology</topic><topic>Animals, Genetically Modified - metabolism</topic><topic>Animals, Genetically Modified - microbiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Body composition</topic><topic>Catfish</topic><topic>Delta-5 Fatty Acid Desaturase - genetics</topic><topic>Delta-5 Fatty Acid Desaturase - metabolism</topic><topic>Desaturase</topic><topic>Disease resistance</topic><topic>Fatty acid composition</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Flavobacterium - physiology</topic><topic>Genetic Engineering</topic><topic>Ictaluridae - growth & development</topic><topic>Ictaluridae - immunology</topic><topic>Ictaluridae - metabolism</topic><topic>Ictaluridae - microbiology</topic><topic>Ictalurus punctatus</topic><topic>Life Sciences</topic><topic>Molecular Medicine</topic><topic>Nutritive value</topic><topic>Oncorhynchus masou</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Transgenes</topic><topic>Transgenic fish</topic><topic>Transgenics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yingqi</creatorcontrib><creatorcontrib>Bugg, William</creatorcontrib><creatorcontrib>Bangs, Max</creatorcontrib><creatorcontrib>Qin, Guyu</creatorcontrib><creatorcontrib>Drescher, David</creatorcontrib><creatorcontrib>Backenstose, Nathan</creatorcontrib><creatorcontrib>Weng, Chia Chen</creatorcontrib><creatorcontrib>Zhang, Yiliu</creatorcontrib><creatorcontrib>Khalil, Karim</creatorcontrib><creatorcontrib>Dong, Sheng</creatorcontrib><creatorcontrib>Elaswad, Ahmed</creatorcontrib><creatorcontrib>Ye, Zhi</creatorcontrib><creatorcontrib>Lu, Cuiyu</creatorcontrib><creatorcontrib>Vo, Khoi</creatorcontrib><creatorcontrib>Simora, Rhoda Mae</creatorcontrib><creatorcontrib>Ma, Xiaoli</creatorcontrib><creatorcontrib>Taylor, Zachary</creatorcontrib><creatorcontrib>Yang, Yujia</creatorcontrib><creatorcontrib>Zhou, Tao</creatorcontrib><creatorcontrib>Guo, Jingping</creatorcontrib><creatorcontrib>Salze, Guillaume</creatorcontrib><creatorcontrib>Qin, Zhenkui</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Dunham, Rex A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yingqi</au><au>Bugg, William</au><au>Bangs, Max</au><au>Qin, Guyu</au><au>Drescher, David</au><au>Backenstose, Nathan</au><au>Weng, Chia Chen</au><au>Zhang, Yiliu</au><au>Khalil, Karim</au><au>Dong, Sheng</au><au>Elaswad, Ahmed</au><au>Ye, Zhi</au><au>Lu, Cuiyu</au><au>Vo, Khoi</au><au>Simora, Rhoda Mae</au><au>Ma, Xiaoli</au><au>Taylor, Zachary</au><au>Yang, Yujia</au><au>Zhou, Tao</au><au>Guo, Jingping</au><au>Salze, Guillaume</au><au>Qin, Zhenkui</au><au>Wang, Yi</au><au>Dunham, Rex A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>30</volume><issue>2</issue><spage>185</spage><epage>200</epage><pages>185-200</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Channel catfish (
Ictalurus punctatus
) is the primary culture species in the US along with its hybrid made with male blue catfish,
I. furcatus
. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (
P
< 0.01,
P
< 0.01,
P
< 0.01). However, while the relative proportion of n-3 fatty acids was achieved, the total amount of fatty acids in the transgenic fish decreased resulting in a reduction of all fatty acids. Insertion of the βactin-D5D transgene into channel catfish also had large effects on the body composition, and growth of channel catfish. Transgenic channel catfish grew faster, were more disease resistant, had higher protein and moisture percentage, but lower fat percentage than full-sib controls. There were sex effects as performance changes were more dramatic and significant in males. The βactin-D5D transgenic channel catfish were also more uniform in their fatty acid composition, growth and other traits.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>33792795</pmid><doi>10.1007/s11248-021-00242-1</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-6149-6017</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0962-8819 |
| ispartof | Transgenic research, 2021-04, Vol.30 (2), p.185-200 |
| issn | 0962-8819 1573-9368 |
| language | eng |
| recordid | cdi_proquest_journals_2509430026 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Acid production Actin Animal Genetics and Genomics Animals Animals, Genetically Modified - growth & development Animals, Genetically Modified - immunology Animals, Genetically Modified - metabolism Animals, Genetically Modified - microbiology Biomedical and Life Sciences Biomedical Engineering/Biotechnology Body composition Catfish Delta-5 Fatty Acid Desaturase - genetics Delta-5 Fatty Acid Desaturase - metabolism Desaturase Disease resistance Fatty acid composition Fatty acids Fatty Acids - metabolism Fish Proteins - genetics Fish Proteins - metabolism Flavobacterium - physiology Genetic Engineering Ictaluridae - growth & development Ictaluridae - immunology Ictaluridae - metabolism Ictaluridae - microbiology Ictalurus punctatus Life Sciences Molecular Medicine Nutritive value Oncorhynchus masou Original Paper Plant Genetics and Genomics Transgenes Transgenic fish Transgenics |
| title | Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T23%3A06%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20and%20pleiotropic%20effects%20of%20the%20Masou%20Salmon%20Delta-5%C2%A0Desaturase%20transgene%20in%20F1%20channel%20catfish%20(Ictalurus%20punctatus)&rft.jtitle=Transgenic%20research&rft.au=Huang,%20Yingqi&rft.date=2021-04-01&rft.volume=30&rft.issue=2&rft.spage=185&rft.epage=200&rft.pages=185-200&rft.issn=0962-8819&rft.eissn=1573-9368&rft_id=info:doi/10.1007/s11248-021-00242-1&rft_dat=%3Cproquest_cross%3E2509430026%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2509430026&rft_id=info:pmid/33792795&rfr_iscdi=true |