Basophil activation test for Staphylococcus aureus enterotoxins in severe asthmatic patients
Background Several studies have shown an association between severe asthma and serum immunoglobulins E (IgE) against Staphylococcus aureus enterotoxins (SEs). SEs—the prototypes being types A (SEA), B (SEB) and toxic shock syndrome toxin 1 (TSST‐1)—can induce both polyclonal and specific IgE respons...
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| Veröffentlicht in: | Clinical and experimental allergy 2021-04, Vol.51 (4), p.536-545 |
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| creator | Flora, Martina Perna, Francesco Abbadessa, Salvatore Garziano, Federica Maffucci, Rosalba Maniscalco, Mauro Mollica, Mariano Pelaia, Corrado Tremante, Eugenio Maffei, Marianna Calabrese, Cecilia |
| description | Background
Several studies have shown an association between severe asthma and serum immunoglobulins E (IgE) against Staphylococcus aureus enterotoxins (SEs). SEs—the prototypes being types A (SEA), B (SEB) and toxic shock syndrome toxin 1 (TSST‐1)—can induce both polyclonal and specific IgE responses.
Objective
The aim of the study was to evaluate the ability of SEs to induce basophil activation in severe asthmatic patients using the basophil activation test (BAT).
Methods
57 severe asthmatic patients were enrolled. BAT in response to SEA, SEB and TSST‐1 was performed in all patients, while serum IgE to SEA, SEB and SEC was available in 49 patients. BAT was considered positive when CD203c+ basophils to SEs were ≥5%, and the stimulation index (SI, ratio between % of CD203c+ basophils to SEs and to negative control) was >2. Two threshold values (>0.1 kU/L and >0.35 kU/L, respectively) were used to assess serum SEsIgE.
Results
36.8% of severe asthmatic patients had a BAT positive for at least one SE (BAT SEs+). Serum SEsIgE >0.35 kU/L (SEs IgE+) was associated with BAT SEs positivity. Among patients with negative skin prick test, 35% were BAT SEs+, 30% SEs IgE+, 55% BAT or IgE− SEs+. A negative correlation between SI of BAT to SEs and both clinical (ACT score) and functional parameters was observed, together with a positive correlation of BAT with asthma exacerbations.
Conclusions
The positivity of BAT for SEs in a subgroup of severe asthmatic patients further supports the pathogenic role of Staphylococcus aureus in severe asthma. |
| doi_str_mv | 10.1111/cea.13772 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2509259630</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2509259630</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3532-e32b871c35de2e81132469d78de467d69aba5b49be02e2791fd54d469506528c3</originalsourceid><addsrcrecordid>eNp1kD1PwzAQhi0EoqUw8AeQJSaGtP6Ik3gsVfmQKjEAG5LlOBc1VRoHOyn032NIYeOGO1l67jnrReiSkikNNTOgp5SnKTtCY8oTEbFQx2hMpIijNJPxCJ15vyGEcCGzUzTinPKwyMbo7VZ7266rGmvTVTvdVbbBHfgOl9bh5063631tjTWm91j3DsKApgNnO_tZNR5XDfawAwdY-269DQKD29AD5M_RSalrDxeHOUGvd8uXxUO0erp_XMxXkeGCswg4y7OUhkcBDDJKOYsTWaRZAXGSFonUuRZ5LHMgDFgqaVmIuAiIIIlgmeETdD14W2ff-_B5tbG9a8JJxQSRTMiEk0DdDJRx1nsHpWpdtdVuryhR3zmqkKP6yTGwVwdjn2-h-CN_gwvAbAA-qhr2_5vUYjkflF-Y-3zE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2509259630</pqid></control><display><type>article</type><title>Basophil activation test for Staphylococcus aureus enterotoxins in severe asthmatic patients</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><creator>Flora, Martina ; Perna, Francesco ; Abbadessa, Salvatore ; Garziano, Federica ; Maffucci, Rosalba ; Maniscalco, Mauro ; Mollica, Mariano ; Pelaia, Corrado ; Tremante, Eugenio ; Maffei, Marianna ; Calabrese, Cecilia</creator><creatorcontrib>Flora, Martina ; Perna, Francesco ; Abbadessa, Salvatore ; Garziano, Federica ; Maffucci, Rosalba ; Maniscalco, Mauro ; Mollica, Mariano ; Pelaia, Corrado ; Tremante, Eugenio ; Maffei, Marianna ; Calabrese, Cecilia</creatorcontrib><description>Background
Several studies have shown an association between severe asthma and serum immunoglobulins E (IgE) against Staphylococcus aureus enterotoxins (SEs). SEs—the prototypes being types A (SEA), B (SEB) and toxic shock syndrome toxin 1 (TSST‐1)—can induce both polyclonal and specific IgE responses.
Objective
The aim of the study was to evaluate the ability of SEs to induce basophil activation in severe asthmatic patients using the basophil activation test (BAT).
Methods
57 severe asthmatic patients were enrolled. BAT in response to SEA, SEB and TSST‐1 was performed in all patients, while serum IgE to SEA, SEB and SEC was available in 49 patients. BAT was considered positive when CD203c+ basophils to SEs were ≥5%, and the stimulation index (SI, ratio between % of CD203c+ basophils to SEs and to negative control) was >2. Two threshold values (>0.1 kU/L and >0.35 kU/L, respectively) were used to assess serum SEsIgE.
Results
36.8% of severe asthmatic patients had a BAT positive for at least one SE (BAT SEs+). Serum SEsIgE >0.35 kU/L (SEs IgE+) was associated with BAT SEs positivity. Among patients with negative skin prick test, 35% were BAT SEs+, 30% SEs IgE+, 55% BAT or IgE− SEs+. A negative correlation between SI of BAT to SEs and both clinical (ACT score) and functional parameters was observed, together with a positive correlation of BAT with asthma exacerbations.
Conclusions
The positivity of BAT for SEs in a subgroup of severe asthmatic patients further supports the pathogenic role of Staphylococcus aureus in severe asthma.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/cea.13772</identifier><identifier>PMID: 33131112</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Asthma ; Asthma - immunology ; Bacterial Toxins - immunology ; basophil activation test ; Basophil Degranulation Test ; disability ; Enterotoxins ; Enterotoxins - immunology ; Female ; Humans ; Immunoglobulin E ; Immunoglobulin E - immunology ; Leukocytes (basophilic) ; Male ; Middle Aged ; outcome ; Septic shock ; severe asthma ; Severity of Illness Index ; Skin Tests ; Staphylococcal enterotoxin F ; Staphylococcus aureus ; Staphylococcus aureus - immunology ; Staphylococcus aureus enterotoxins ; Superantigens - immunology ; Toxic shock syndrome</subject><ispartof>Clinical and experimental allergy, 2021-04, Vol.51 (4), p.536-545</ispartof><rights>2020 John Wiley & Sons Ltd</rights><rights>2020 John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-e32b871c35de2e81132469d78de467d69aba5b49be02e2791fd54d469506528c3</citedby><cites>FETCH-LOGICAL-c3532-e32b871c35de2e81132469d78de467d69aba5b49be02e2791fd54d469506528c3</cites><orcidid>0000-0002-4236-7367 ; 0000-0001-6751-9921 ; 0000-0003-4293-4461</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fcea.13772$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fcea.13772$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33131112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flora, Martina</creatorcontrib><creatorcontrib>Perna, Francesco</creatorcontrib><creatorcontrib>Abbadessa, Salvatore</creatorcontrib><creatorcontrib>Garziano, Federica</creatorcontrib><creatorcontrib>Maffucci, Rosalba</creatorcontrib><creatorcontrib>Maniscalco, Mauro</creatorcontrib><creatorcontrib>Mollica, Mariano</creatorcontrib><creatorcontrib>Pelaia, Corrado</creatorcontrib><creatorcontrib>Tremante, Eugenio</creatorcontrib><creatorcontrib>Maffei, Marianna</creatorcontrib><creatorcontrib>Calabrese, Cecilia</creatorcontrib><title>Basophil activation test for Staphylococcus aureus enterotoxins in severe asthmatic patients</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Background
Several studies have shown an association between severe asthma and serum immunoglobulins E (IgE) against Staphylococcus aureus enterotoxins (SEs). SEs—the prototypes being types A (SEA), B (SEB) and toxic shock syndrome toxin 1 (TSST‐1)—can induce both polyclonal and specific IgE responses.
Objective
The aim of the study was to evaluate the ability of SEs to induce basophil activation in severe asthmatic patients using the basophil activation test (BAT).
Methods
57 severe asthmatic patients were enrolled. BAT in response to SEA, SEB and TSST‐1 was performed in all patients, while serum IgE to SEA, SEB and SEC was available in 49 patients. BAT was considered positive when CD203c+ basophils to SEs were ≥5%, and the stimulation index (SI, ratio between % of CD203c+ basophils to SEs and to negative control) was >2. Two threshold values (>0.1 kU/L and >0.35 kU/L, respectively) were used to assess serum SEsIgE.
Results
36.8% of severe asthmatic patients had a BAT positive for at least one SE (BAT SEs+). Serum SEsIgE >0.35 kU/L (SEs IgE+) was associated with BAT SEs positivity. Among patients with negative skin prick test, 35% were BAT SEs+, 30% SEs IgE+, 55% BAT or IgE− SEs+. A negative correlation between SI of BAT to SEs and both clinical (ACT score) and functional parameters was observed, together with a positive correlation of BAT with asthma exacerbations.
Conclusions
The positivity of BAT for SEs in a subgroup of severe asthmatic patients further supports the pathogenic role of Staphylococcus aureus in severe asthma.</description><subject>Adult</subject><subject>Aged</subject><subject>Asthma</subject><subject>Asthma - immunology</subject><subject>Bacterial Toxins - immunology</subject><subject>basophil activation test</subject><subject>Basophil Degranulation Test</subject><subject>disability</subject><subject>Enterotoxins</subject><subject>Enterotoxins - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin E - immunology</subject><subject>Leukocytes (basophilic)</subject><subject>Male</subject><subject>Middle Aged</subject><subject>outcome</subject><subject>Septic shock</subject><subject>severe asthma</subject><subject>Severity of Illness Index</subject><subject>Skin Tests</subject><subject>Staphylococcal enterotoxin F</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - immunology</subject><subject>Staphylococcus aureus enterotoxins</subject><subject>Superantigens - immunology</subject><subject>Toxic shock syndrome</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EoqUw8AeQJSaGtP6Ik3gsVfmQKjEAG5LlOBc1VRoHOyn032NIYeOGO1l67jnrReiSkikNNTOgp5SnKTtCY8oTEbFQx2hMpIijNJPxCJ15vyGEcCGzUzTinPKwyMbo7VZ7266rGmvTVTvdVbbBHfgOl9bh5063631tjTWm91j3DsKApgNnO_tZNR5XDfawAwdY-269DQKD29AD5M_RSalrDxeHOUGvd8uXxUO0erp_XMxXkeGCswg4y7OUhkcBDDJKOYsTWaRZAXGSFonUuRZ5LHMgDFgqaVmIuAiIIIlgmeETdD14W2ff-_B5tbG9a8JJxQSRTMiEk0DdDJRx1nsHpWpdtdVuryhR3zmqkKP6yTGwVwdjn2-h-CN_gwvAbAA-qhr2_5vUYjkflF-Y-3zE</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Flora, Martina</creator><creator>Perna, Francesco</creator><creator>Abbadessa, Salvatore</creator><creator>Garziano, Federica</creator><creator>Maffucci, Rosalba</creator><creator>Maniscalco, Mauro</creator><creator>Mollica, Mariano</creator><creator>Pelaia, Corrado</creator><creator>Tremante, Eugenio</creator><creator>Maffei, Marianna</creator><creator>Calabrese, Cecilia</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-4236-7367</orcidid><orcidid>https://orcid.org/0000-0001-6751-9921</orcidid><orcidid>https://orcid.org/0000-0003-4293-4461</orcidid></search><sort><creationdate>202104</creationdate><title>Basophil activation test for Staphylococcus aureus enterotoxins in severe asthmatic patients</title><author>Flora, Martina ; Perna, Francesco ; Abbadessa, Salvatore ; Garziano, Federica ; Maffucci, Rosalba ; Maniscalco, Mauro ; Mollica, Mariano ; Pelaia, Corrado ; Tremante, Eugenio ; Maffei, Marianna ; Calabrese, Cecilia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-e32b871c35de2e81132469d78de467d69aba5b49be02e2791fd54d469506528c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Asthma</topic><topic>Asthma - immunology</topic><topic>Bacterial Toxins - immunology</topic><topic>basophil activation test</topic><topic>Basophil Degranulation Test</topic><topic>disability</topic><topic>Enterotoxins</topic><topic>Enterotoxins - immunology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin E - immunology</topic><topic>Leukocytes (basophilic)</topic><topic>Male</topic><topic>Middle Aged</topic><topic>outcome</topic><topic>Septic shock</topic><topic>severe asthma</topic><topic>Severity of Illness Index</topic><topic>Skin Tests</topic><topic>Staphylococcal enterotoxin F</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - immunology</topic><topic>Staphylococcus aureus enterotoxins</topic><topic>Superantigens - immunology</topic><topic>Toxic shock syndrome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flora, Martina</creatorcontrib><creatorcontrib>Perna, Francesco</creatorcontrib><creatorcontrib>Abbadessa, Salvatore</creatorcontrib><creatorcontrib>Garziano, Federica</creatorcontrib><creatorcontrib>Maffucci, Rosalba</creatorcontrib><creatorcontrib>Maniscalco, Mauro</creatorcontrib><creatorcontrib>Mollica, Mariano</creatorcontrib><creatorcontrib>Pelaia, Corrado</creatorcontrib><creatorcontrib>Tremante, Eugenio</creatorcontrib><creatorcontrib>Maffei, Marianna</creatorcontrib><creatorcontrib>Calabrese, Cecilia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flora, Martina</au><au>Perna, Francesco</au><au>Abbadessa, Salvatore</au><au>Garziano, Federica</au><au>Maffucci, Rosalba</au><au>Maniscalco, Mauro</au><au>Mollica, Mariano</au><au>Pelaia, Corrado</au><au>Tremante, Eugenio</au><au>Maffei, Marianna</au><au>Calabrese, Cecilia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Basophil activation test for Staphylococcus aureus enterotoxins in severe asthmatic patients</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2021-04</date><risdate>2021</risdate><volume>51</volume><issue>4</issue><spage>536</spage><epage>545</epage><pages>536-545</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Background
Several studies have shown an association between severe asthma and serum immunoglobulins E (IgE) against Staphylococcus aureus enterotoxins (SEs). SEs—the prototypes being types A (SEA), B (SEB) and toxic shock syndrome toxin 1 (TSST‐1)—can induce both polyclonal and specific IgE responses.
Objective
The aim of the study was to evaluate the ability of SEs to induce basophil activation in severe asthmatic patients using the basophil activation test (BAT).
Methods
57 severe asthmatic patients were enrolled. BAT in response to SEA, SEB and TSST‐1 was performed in all patients, while serum IgE to SEA, SEB and SEC was available in 49 patients. BAT was considered positive when CD203c+ basophils to SEs were ≥5%, and the stimulation index (SI, ratio between % of CD203c+ basophils to SEs and to negative control) was >2. Two threshold values (>0.1 kU/L and >0.35 kU/L, respectively) were used to assess serum SEsIgE.
Results
36.8% of severe asthmatic patients had a BAT positive for at least one SE (BAT SEs+). Serum SEsIgE >0.35 kU/L (SEs IgE+) was associated with BAT SEs positivity. Among patients with negative skin prick test, 35% were BAT SEs+, 30% SEs IgE+, 55% BAT or IgE− SEs+. A negative correlation between SI of BAT to SEs and both clinical (ACT score) and functional parameters was observed, together with a positive correlation of BAT with asthma exacerbations.
Conclusions
The positivity of BAT for SEs in a subgroup of severe asthmatic patients further supports the pathogenic role of Staphylococcus aureus in severe asthma.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33131112</pmid><doi>10.1111/cea.13772</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4236-7367</orcidid><orcidid>https://orcid.org/0000-0001-6751-9921</orcidid><orcidid>https://orcid.org/0000-0003-4293-4461</orcidid></addata></record> |
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| ispartof | Clinical and experimental allergy, 2021-04, Vol.51 (4), p.536-545 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Adult Aged Asthma Asthma - immunology Bacterial Toxins - immunology basophil activation test Basophil Degranulation Test disability Enterotoxins Enterotoxins - immunology Female Humans Immunoglobulin E Immunoglobulin E - immunology Leukocytes (basophilic) Male Middle Aged outcome Septic shock severe asthma Severity of Illness Index Skin Tests Staphylococcal enterotoxin F Staphylococcus aureus Staphylococcus aureus - immunology Staphylococcus aureus enterotoxins Superantigens - immunology Toxic shock syndrome |
| title | Basophil activation test for Staphylococcus aureus enterotoxins in severe asthmatic patients |
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