Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats
Our previous study showed that intravitreal delivery of self-complementary AAV2 (scAAV2)-mediated exoenzyme C3 transferase (C3) can attenuate retinal ischemia/reperfusion (I/R) injury. The current study investigated the neuroprotective effects of lentivirus (LV)-mediated C3 transgene expression on r...
Gespeichert in:
| Veröffentlicht in: | Life sciences (1973) 2021-05, Vol.272, p.119269, Article 119269 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 119269 |
| container_title | Life sciences (1973) |
| container_volume | 272 |
| creator | Tan, Junkai Liu, Guo Lan, Chunlin Pang, Iok-Hou Luo, Xiaolin Wu, Shen Fan, Ning Zhang, Jingxue Wang, Ningli Liu, Xuyang |
| description | Our previous study showed that intravitreal delivery of self-complementary AAV2 (scAAV2)-mediated exoenzyme C3 transferase (C3) can attenuate retinal ischemia/reperfusion (I/R) injury. The current study investigated the neuroprotective effects of lentivirus (LV)-mediated C3 transgene expression on rat retinal I/R injury.
The LV encoding C3 and green fluorescent protein (GFP) together (LV-C3-GFP) or GFP only (LV-GFP) was intravitreally injected to SPRAGUE-DAWLEY rats. On day 5 post-intravitreal injection, eyes were evaluated by slit-lamp examination. The GFP expression on retina was confirmed by in vivo and ex vivo assessments. RhoA GTPase expression in retina was examined by western blot. Retinal I/R injury was generated by transiently increasing intraocular pressure (110 mmHg, 90 min). Eyes were then enucleated, and retinas processed for morphological analysis and TdT-dUTP terminal nick-end labeling (TUNEL) assay.
No obvious inflammatory reactions or surgical complications were observed after intravitreal injection of LV vectors. There was a significant decrease of total RhoA GTPase level in the retina treated with LV-C3-GFP. Compared to the blank control group, LV-C3-GFP and LV-GFP did not affect the retinal thickness, cell density in ganglion cell layer (GCL), or numbers of apoptotic cells in retinal flat-mounts. In the LV-GFP-treated retinas, I/R decreased the retinal thickness and GCL cell density and increased apoptotic retinal cell numbers. LV-C3-GFP significantly protected against all these degenerative effects of I/R.
This study indicated that LV-mediated C3 transgene expression exhibits neuroprotective effects on the retinal I/R injury and holds potential as a novel neuroprotective approach targeting certain retinopathies. |
| doi_str_mv | 10.1016/j.lfs.2021.119269 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2508913415</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S002432052100254X</els_id><sourcerecordid>2508913415</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-85c8ceb56507709d3fa695eec445c53225a8e5750477dbe8d3c1171464289e1f3</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMo7rr6A7xIwHNrPpp-4EkWv2DBi55DNp1iym5bJ-mi_96sXT16Ghie92XmIeSSs5Qznt-06abxqWCCp5xXIq-OyJyXRZWwXPJjMmdMZIkUTM3ImfctY0ypQp6SmZQR4IWaE1xBF9zOodnQHdjQY7KF2pkANYXPAcF713e0b-hS0oCm8w2g8UBNCNCNkfMUIbgu5p2377B1hpqujssBsBl_0q5rR_yKg6IJ_pycNGbj4eIwF-Tt4f51-ZSsXh6fl3erxMqSh6RUtrSwVrliRcGqWjYmrxSAzTJllRRCmRJUoVhWFPUaylra-BLP8kyUFfBGLsj11Dtg_zGCD7rtR4yHei0UKysuM64ixSfKYu89QqMHdFuDX5ozvbesWx0t671lPVmOmatD87iOtv4Sv1ojcDsBEP_bOUDtrYPORrMYJeu6d__UfwOovo4c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2508913415</pqid></control><display><type>article</type><title>Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tan, Junkai ; Liu, Guo ; Lan, Chunlin ; Pang, Iok-Hou ; Luo, Xiaolin ; Wu, Shen ; Fan, Ning ; Zhang, Jingxue ; Wang, Ningli ; Liu, Xuyang</creator><creatorcontrib>Tan, Junkai ; Liu, Guo ; Lan, Chunlin ; Pang, Iok-Hou ; Luo, Xiaolin ; Wu, Shen ; Fan, Ning ; Zhang, Jingxue ; Wang, Ningli ; Liu, Xuyang</creatorcontrib><description>Our previous study showed that intravitreal delivery of self-complementary AAV2 (scAAV2)-mediated exoenzyme C3 transferase (C3) can attenuate retinal ischemia/reperfusion (I/R) injury. The current study investigated the neuroprotective effects of lentivirus (LV)-mediated C3 transgene expression on rat retinal I/R injury.
The LV encoding C3 and green fluorescent protein (GFP) together (LV-C3-GFP) or GFP only (LV-GFP) was intravitreally injected to SPRAGUE-DAWLEY rats. On day 5 post-intravitreal injection, eyes were evaluated by slit-lamp examination. The GFP expression on retina was confirmed by in vivo and ex vivo assessments. RhoA GTPase expression in retina was examined by western blot. Retinal I/R injury was generated by transiently increasing intraocular pressure (110 mmHg, 90 min). Eyes were then enucleated, and retinas processed for morphological analysis and TdT-dUTP terminal nick-end labeling (TUNEL) assay.
No obvious inflammatory reactions or surgical complications were observed after intravitreal injection of LV vectors. There was a significant decrease of total RhoA GTPase level in the retina treated with LV-C3-GFP. Compared to the blank control group, LV-C3-GFP and LV-GFP did not affect the retinal thickness, cell density in ganglion cell layer (GCL), or numbers of apoptotic cells in retinal flat-mounts. In the LV-GFP-treated retinas, I/R decreased the retinal thickness and GCL cell density and increased apoptotic retinal cell numbers. LV-C3-GFP significantly protected against all these degenerative effects of I/R.
This study indicated that LV-mediated C3 transgene expression exhibits neuroprotective effects on the retinal I/R injury and holds potential as a novel neuroprotective approach targeting certain retinopathies.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2021.119269</identifier><identifier>PMID: 33631175</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>ADP Ribose Transferases - metabolism ; ADP Ribose Transferases - pharmacology ; Animals ; Apoptosis ; Apoptosis - drug effects ; Botulinum Toxins - metabolism ; Botulinum Toxins - pharmacology ; Cell density ; Cell Survival - drug effects ; Complications ; Density ; DNA nucleotidylexotransferase ; Exoenzyme C3 transferase ; Eye (anatomy) ; Fluorescence ; Green fluorescent protein ; Green Fluorescent Proteins - metabolism ; Inflammation ; Injection ; Injuries ; Intraocular pressure ; Intraocular Pressure - drug effects ; Ischemia ; Ischemia - metabolism ; Ischemia - therapy ; Ischemia/reperfusion injury ; Lentivirus ; Lentivirus - genetics ; Lentivirus - metabolism ; Male ; Neuroprotection ; Neuroprotective Agents - pharmacology ; Rat ; Rats ; Rats, Sprague-Dawley ; Reperfusion ; Reperfusion Injury - metabolism ; Reperfusion Injury - therapy ; Retina ; Retina - metabolism ; Retinal cells ; Retinal Diseases - metabolism ; Retinal Diseases - therapy ; Retinal Ganglion Cells - metabolism ; RhoA protein ; Thickness</subject><ispartof>Life sciences (1973), 2021-05, Vol.272, p.119269, Article 119269</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV May 1, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-85c8ceb56507709d3fa695eec445c53225a8e5750477dbe8d3c1171464289e1f3</citedby><cites>FETCH-LOGICAL-c381t-85c8ceb56507709d3fa695eec445c53225a8e5750477dbe8d3c1171464289e1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S002432052100254X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33631175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, Junkai</creatorcontrib><creatorcontrib>Liu, Guo</creatorcontrib><creatorcontrib>Lan, Chunlin</creatorcontrib><creatorcontrib>Pang, Iok-Hou</creatorcontrib><creatorcontrib>Luo, Xiaolin</creatorcontrib><creatorcontrib>Wu, Shen</creatorcontrib><creatorcontrib>Fan, Ning</creatorcontrib><creatorcontrib>Zhang, Jingxue</creatorcontrib><creatorcontrib>Wang, Ningli</creatorcontrib><creatorcontrib>Liu, Xuyang</creatorcontrib><title>Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Our previous study showed that intravitreal delivery of self-complementary AAV2 (scAAV2)-mediated exoenzyme C3 transferase (C3) can attenuate retinal ischemia/reperfusion (I/R) injury. The current study investigated the neuroprotective effects of lentivirus (LV)-mediated C3 transgene expression on rat retinal I/R injury.
The LV encoding C3 and green fluorescent protein (GFP) together (LV-C3-GFP) or GFP only (LV-GFP) was intravitreally injected to SPRAGUE-DAWLEY rats. On day 5 post-intravitreal injection, eyes were evaluated by slit-lamp examination. The GFP expression on retina was confirmed by in vivo and ex vivo assessments. RhoA GTPase expression in retina was examined by western blot. Retinal I/R injury was generated by transiently increasing intraocular pressure (110 mmHg, 90 min). Eyes were then enucleated, and retinas processed for morphological analysis and TdT-dUTP terminal nick-end labeling (TUNEL) assay.
No obvious inflammatory reactions or surgical complications were observed after intravitreal injection of LV vectors. There was a significant decrease of total RhoA GTPase level in the retina treated with LV-C3-GFP. Compared to the blank control group, LV-C3-GFP and LV-GFP did not affect the retinal thickness, cell density in ganglion cell layer (GCL), or numbers of apoptotic cells in retinal flat-mounts. In the LV-GFP-treated retinas, I/R decreased the retinal thickness and GCL cell density and increased apoptotic retinal cell numbers. LV-C3-GFP significantly protected against all these degenerative effects of I/R.
This study indicated that LV-mediated C3 transgene expression exhibits neuroprotective effects on the retinal I/R injury and holds potential as a novel neuroprotective approach targeting certain retinopathies.</description><subject>ADP Ribose Transferases - metabolism</subject><subject>ADP Ribose Transferases - pharmacology</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Botulinum Toxins - metabolism</subject><subject>Botulinum Toxins - pharmacology</subject><subject>Cell density</subject><subject>Cell Survival - drug effects</subject><subject>Complications</subject><subject>Density</subject><subject>DNA nucleotidylexotransferase</subject><subject>Exoenzyme C3 transferase</subject><subject>Eye (anatomy)</subject><subject>Fluorescence</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Inflammation</subject><subject>Injection</subject><subject>Injuries</subject><subject>Intraocular pressure</subject><subject>Intraocular Pressure - drug effects</subject><subject>Ischemia</subject><subject>Ischemia - metabolism</subject><subject>Ischemia - therapy</subject><subject>Ischemia/reperfusion injury</subject><subject>Lentivirus</subject><subject>Lentivirus - genetics</subject><subject>Lentivirus - metabolism</subject><subject>Male</subject><subject>Neuroprotection</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - therapy</subject><subject>Retina</subject><subject>Retina - metabolism</subject><subject>Retinal cells</subject><subject>Retinal Diseases - metabolism</subject><subject>Retinal Diseases - therapy</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>RhoA protein</subject><subject>Thickness</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6A7xIwHNrPpp-4EkWv2DBi55DNp1iym5bJ-mi_96sXT16Ghie92XmIeSSs5Qznt-06abxqWCCp5xXIq-OyJyXRZWwXPJjMmdMZIkUTM3ImfctY0ypQp6SmZQR4IWaE1xBF9zOodnQHdjQY7KF2pkANYXPAcF713e0b-hS0oCm8w2g8UBNCNCNkfMUIbgu5p2377B1hpqujssBsBl_0q5rR_yKg6IJ_pycNGbj4eIwF-Tt4f51-ZSsXh6fl3erxMqSh6RUtrSwVrliRcGqWjYmrxSAzTJllRRCmRJUoVhWFPUaylra-BLP8kyUFfBGLsj11Dtg_zGCD7rtR4yHei0UKysuM64ixSfKYu89QqMHdFuDX5ozvbesWx0t671lPVmOmatD87iOtv4Sv1ojcDsBEP_bOUDtrYPORrMYJeu6d__UfwOovo4c</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Tan, Junkai</creator><creator>Liu, Guo</creator><creator>Lan, Chunlin</creator><creator>Pang, Iok-Hou</creator><creator>Luo, Xiaolin</creator><creator>Wu, Shen</creator><creator>Fan, Ning</creator><creator>Zhang, Jingxue</creator><creator>Wang, Ningli</creator><creator>Liu, Xuyang</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20210501</creationdate><title>Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats</title><author>Tan, Junkai ; Liu, Guo ; Lan, Chunlin ; Pang, Iok-Hou ; Luo, Xiaolin ; Wu, Shen ; Fan, Ning ; Zhang, Jingxue ; Wang, Ningli ; Liu, Xuyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-85c8ceb56507709d3fa695eec445c53225a8e5750477dbe8d3c1171464289e1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>ADP Ribose Transferases - metabolism</topic><topic>ADP Ribose Transferases - pharmacology</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Botulinum Toxins - metabolism</topic><topic>Botulinum Toxins - pharmacology</topic><topic>Cell density</topic><topic>Cell Survival - drug effects</topic><topic>Complications</topic><topic>Density</topic><topic>DNA nucleotidylexotransferase</topic><topic>Exoenzyme C3 transferase</topic><topic>Eye (anatomy)</topic><topic>Fluorescence</topic><topic>Green fluorescent protein</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Inflammation</topic><topic>Injection</topic><topic>Injuries</topic><topic>Intraocular pressure</topic><topic>Intraocular Pressure - drug effects</topic><topic>Ischemia</topic><topic>Ischemia - metabolism</topic><topic>Ischemia - therapy</topic><topic>Ischemia/reperfusion injury</topic><topic>Lentivirus</topic><topic>Lentivirus - genetics</topic><topic>Lentivirus - metabolism</topic><topic>Male</topic><topic>Neuroprotection</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion</topic><topic>Reperfusion Injury - metabolism</topic><topic>Reperfusion Injury - therapy</topic><topic>Retina</topic><topic>Retina - metabolism</topic><topic>Retinal cells</topic><topic>Retinal Diseases - metabolism</topic><topic>Retinal Diseases - therapy</topic><topic>Retinal Ganglion Cells - metabolism</topic><topic>RhoA protein</topic><topic>Thickness</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, Junkai</creatorcontrib><creatorcontrib>Liu, Guo</creatorcontrib><creatorcontrib>Lan, Chunlin</creatorcontrib><creatorcontrib>Pang, Iok-Hou</creatorcontrib><creatorcontrib>Luo, Xiaolin</creatorcontrib><creatorcontrib>Wu, Shen</creatorcontrib><creatorcontrib>Fan, Ning</creatorcontrib><creatorcontrib>Zhang, Jingxue</creatorcontrib><creatorcontrib>Wang, Ningli</creatorcontrib><creatorcontrib>Liu, Xuyang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, Junkai</au><au>Liu, Guo</au><au>Lan, Chunlin</au><au>Pang, Iok-Hou</au><au>Luo, Xiaolin</au><au>Wu, Shen</au><au>Fan, Ning</au><au>Zhang, Jingxue</au><au>Wang, Ningli</au><au>Liu, Xuyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>272</volume><spage>119269</spage><pages>119269-</pages><artnum>119269</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Our previous study showed that intravitreal delivery of self-complementary AAV2 (scAAV2)-mediated exoenzyme C3 transferase (C3) can attenuate retinal ischemia/reperfusion (I/R) injury. The current study investigated the neuroprotective effects of lentivirus (LV)-mediated C3 transgene expression on rat retinal I/R injury.
The LV encoding C3 and green fluorescent protein (GFP) together (LV-C3-GFP) or GFP only (LV-GFP) was intravitreally injected to SPRAGUE-DAWLEY rats. On day 5 post-intravitreal injection, eyes were evaluated by slit-lamp examination. The GFP expression on retina was confirmed by in vivo and ex vivo assessments. RhoA GTPase expression in retina was examined by western blot. Retinal I/R injury was generated by transiently increasing intraocular pressure (110 mmHg, 90 min). Eyes were then enucleated, and retinas processed for morphological analysis and TdT-dUTP terminal nick-end labeling (TUNEL) assay.
No obvious inflammatory reactions or surgical complications were observed after intravitreal injection of LV vectors. There was a significant decrease of total RhoA GTPase level in the retina treated with LV-C3-GFP. Compared to the blank control group, LV-C3-GFP and LV-GFP did not affect the retinal thickness, cell density in ganglion cell layer (GCL), or numbers of apoptotic cells in retinal flat-mounts. In the LV-GFP-treated retinas, I/R decreased the retinal thickness and GCL cell density and increased apoptotic retinal cell numbers. LV-C3-GFP significantly protected against all these degenerative effects of I/R.
This study indicated that LV-mediated C3 transgene expression exhibits neuroprotective effects on the retinal I/R injury and holds potential as a novel neuroprotective approach targeting certain retinopathies.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>33631175</pmid><doi>10.1016/j.lfs.2021.119269</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 2021-05, Vol.272, p.119269, Article 119269 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_journals_2508913415 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ADP Ribose Transferases - metabolism ADP Ribose Transferases - pharmacology Animals Apoptosis Apoptosis - drug effects Botulinum Toxins - metabolism Botulinum Toxins - pharmacology Cell density Cell Survival - drug effects Complications Density DNA nucleotidylexotransferase Exoenzyme C3 transferase Eye (anatomy) Fluorescence Green fluorescent protein Green Fluorescent Proteins - metabolism Inflammation Injection Injuries Intraocular pressure Intraocular Pressure - drug effects Ischemia Ischemia - metabolism Ischemia - therapy Ischemia/reperfusion injury Lentivirus Lentivirus - genetics Lentivirus - metabolism Male Neuroprotection Neuroprotective Agents - pharmacology Rat Rats Rats, Sprague-Dawley Reperfusion Reperfusion Injury - metabolism Reperfusion Injury - therapy Retina Retina - metabolism Retinal cells Retinal Diseases - metabolism Retinal Diseases - therapy Retinal Ganglion Cells - metabolism RhoA protein Thickness |
| title | Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T11%3A46%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lentiviral%20vector-mediated%20expression%20of%20C3%20transferase%20attenuates%20retinal%20ischemia%20and%20reperfusion%20injury%20in%20rats&rft.jtitle=Life%20sciences%20(1973)&rft.au=Tan,%20Junkai&rft.date=2021-05-01&rft.volume=272&rft.spage=119269&rft.pages=119269-&rft.artnum=119269&rft.issn=0024-3205&rft.eissn=1879-0631&rft_id=info:doi/10.1016/j.lfs.2021.119269&rft_dat=%3Cproquest_cross%3E2508913415%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2508913415&rft_id=info:pmid/33631175&rft_els_id=S002432052100254X&rfr_iscdi=true |