The Role of Phytohormones in the Induction of Somatic Embryogenesis in Pinus sibirica and Larix sibirica
The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in...
Gespeichert in:
| Veröffentlicht in: | CYTOLOGIA 2021/03/25, Vol.86(1), pp.55-60 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 60 |
|---|---|
| container_issue | 1 |
| container_start_page | 55 |
| container_title | CYTOLOGIA |
| container_volume | 86 |
| creator | Tretyakova, Iraida N. Shuklina, Alla S. Park, Maria E. Yang, Ling Akhiyarova, Guzel R. Kudoyarova, Guzel R. |
| description | The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells. |
| doi_str_mv | 10.1508/cytologia.86.55 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2507996556</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2507996556</sourcerecordid><originalsourceid>FETCH-LOGICAL-c544t-bf30f30497441c0c58cefd9f90c66b7ad52ed2ee84ffe1fb40a97105476d63e23</originalsourceid><addsrcrecordid>eNpVkN9LwzAQx4MoOHTPvgZ87ry0Sdo8yvzNQNH5HNI0WTPWRpMW3H9v5mQiHHdw9_l-D74IXRCYEQbVld4OfuNXTs0qPmPsCE1IQausBCKO0QSAkIwyyk7RNEZXA-S8BFHmE9QuW4Nf_cZgb_FLm2xaHzrfm4hdj4d0fOybUQ_O9zvizXdqcBrfdnXY-pVJnPshX1w_Rpy8XXBaYdU3eKGC-zqsztGJVZtopr_zDL3f3S7nD9ni-f5xfr3INKN0yGpbQCoqSkqJBs0qbWwjrADNeV2qhuWmyY2pqLWG2JqCEiUBRkve8MLkxRm63Pt-BP85mjjItR9Dn17KnEEpBGeMJ-pqT-ngYwzGyo_gOhW2koDcJSoPicqKS8aS4mmvWMdBrcyBVyHlsTH_ebJrNzIHCQAC_qBWBWn64hsO9Ie6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2507996556</pqid></control><display><type>article</type><title>The Role of Phytohormones in the Induction of Somatic Embryogenesis in Pinus sibirica and Larix sibirica</title><source>J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese</source><creator>Tretyakova, Iraida N. ; Shuklina, Alla S. ; Park, Maria E. ; Yang, Ling ; Akhiyarova, Guzel R. ; Kudoyarova, Guzel R.</creator><creatorcontrib>Tretyakova, Iraida N. ; Shuklina, Alla S. ; Park, Maria E. ; Yang, Ling ; Akhiyarova, Guzel R. ; Kudoyarova, Guzel R.</creatorcontrib><description>The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells.</description><identifier>ISSN: 0011-4545</identifier><identifier>EISSN: 1348-7019</identifier><identifier>DOI: 10.1508/cytologia.86.55</identifier><language>eng</language><publisher>Tokyo: Japan Mendel Society, International Society of Cytology</publisher><subject>Abscisic acid ; Acetic acid ; Callus ; Cytokinins ; Elongation ; Embryonic growth stage ; Embryos ; Explants ; Hormones ; Indoleacetic acid ; Indolylacetic acid ; Larix sibirica ; Localization ; Phytohormones ; Pinus sibirica ; Plant hormones ; Somatic cells ; Somatic embryogenesis</subject><ispartof>CYTOLOGIA, 2021/03/25, Vol.86(1), pp.55-60</ispartof><rights>2021 The Japan Mendel Society</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-bf30f30497441c0c58cefd9f90c66b7ad52ed2ee84ffe1fb40a97105476d63e23</citedby><cites>FETCH-LOGICAL-c544t-bf30f30497441c0c58cefd9f90c66b7ad52ed2ee84ffe1fb40a97105476d63e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids></links><search><creatorcontrib>Tretyakova, Iraida N.</creatorcontrib><creatorcontrib>Shuklina, Alla S.</creatorcontrib><creatorcontrib>Park, Maria E.</creatorcontrib><creatorcontrib>Yang, Ling</creatorcontrib><creatorcontrib>Akhiyarova, Guzel R.</creatorcontrib><creatorcontrib>Kudoyarova, Guzel R.</creatorcontrib><title>The Role of Phytohormones in the Induction of Somatic Embryogenesis in Pinus sibirica and Larix sibirica</title><title>CYTOLOGIA</title><description>The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells.</description><subject>Abscisic acid</subject><subject>Acetic acid</subject><subject>Callus</subject><subject>Cytokinins</subject><subject>Elongation</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Explants</subject><subject>Hormones</subject><subject>Indoleacetic acid</subject><subject>Indolylacetic acid</subject><subject>Larix sibirica</subject><subject>Localization</subject><subject>Phytohormones</subject><subject>Pinus sibirica</subject><subject>Plant hormones</subject><subject>Somatic cells</subject><subject>Somatic embryogenesis</subject><issn>0011-4545</issn><issn>1348-7019</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpVkN9LwzAQx4MoOHTPvgZ87ry0Sdo8yvzNQNH5HNI0WTPWRpMW3H9v5mQiHHdw9_l-D74IXRCYEQbVld4OfuNXTs0qPmPsCE1IQausBCKO0QSAkIwyyk7RNEZXA-S8BFHmE9QuW4Nf_cZgb_FLm2xaHzrfm4hdj4d0fOybUQ_O9zvizXdqcBrfdnXY-pVJnPshX1w_Rpy8XXBaYdU3eKGC-zqsztGJVZtopr_zDL3f3S7nD9ni-f5xfr3INKN0yGpbQCoqSkqJBs0qbWwjrADNeV2qhuWmyY2pqLWG2JqCEiUBRkve8MLkxRm63Pt-BP85mjjItR9Dn17KnEEpBGeMJ-pqT-ngYwzGyo_gOhW2koDcJSoPicqKS8aS4mmvWMdBrcyBVyHlsTH_ebJrNzIHCQAC_qBWBWn64hsO9Ie6</recordid><startdate>20210325</startdate><enddate>20210325</enddate><creator>Tretyakova, Iraida N.</creator><creator>Shuklina, Alla S.</creator><creator>Park, Maria E.</creator><creator>Yang, Ling</creator><creator>Akhiyarova, Guzel R.</creator><creator>Kudoyarova, Guzel R.</creator><general>Japan Mendel Society, International Society of Cytology</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20210325</creationdate><title>The Role of Phytohormones in the Induction of Somatic Embryogenesis in Pinus sibirica and Larix sibirica</title><author>Tretyakova, Iraida N. ; Shuklina, Alla S. ; Park, Maria E. ; Yang, Ling ; Akhiyarova, Guzel R. ; Kudoyarova, Guzel R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c544t-bf30f30497441c0c58cefd9f90c66b7ad52ed2ee84ffe1fb40a97105476d63e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Abscisic acid</topic><topic>Acetic acid</topic><topic>Callus</topic><topic>Cytokinins</topic><topic>Elongation</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Explants</topic><topic>Hormones</topic><topic>Indoleacetic acid</topic><topic>Indolylacetic acid</topic><topic>Larix sibirica</topic><topic>Localization</topic><topic>Phytohormones</topic><topic>Pinus sibirica</topic><topic>Plant hormones</topic><topic>Somatic cells</topic><topic>Somatic embryogenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tretyakova, Iraida N.</creatorcontrib><creatorcontrib>Shuklina, Alla S.</creatorcontrib><creatorcontrib>Park, Maria E.</creatorcontrib><creatorcontrib>Yang, Ling</creatorcontrib><creatorcontrib>Akhiyarova, Guzel R.</creatorcontrib><creatorcontrib>Kudoyarova, Guzel R.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>CYTOLOGIA</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tretyakova, Iraida N.</au><au>Shuklina, Alla S.</au><au>Park, Maria E.</au><au>Yang, Ling</au><au>Akhiyarova, Guzel R.</au><au>Kudoyarova, Guzel R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of Phytohormones in the Induction of Somatic Embryogenesis in Pinus sibirica and Larix sibirica</atitle><jtitle>CYTOLOGIA</jtitle><date>2021-03-25</date><risdate>2021</risdate><volume>86</volume><issue>1</issue><spage>55</spage><epage>60</epage><pages>55-60</pages><issn>0011-4545</issn><eissn>1348-7019</eissn><abstract>The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells.</abstract><cop>Tokyo</cop><pub>Japan Mendel Society, International Society of Cytology</pub><doi>10.1508/cytologia.86.55</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-4545 |
| ispartof | CYTOLOGIA, 2021/03/25, Vol.86(1), pp.55-60 |
| issn | 0011-4545 1348-7019 |
| language | eng |
| recordid | cdi_proquest_journals_2507996556 |
| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Abscisic acid Acetic acid Callus Cytokinins Elongation Embryonic growth stage Embryos Explants Hormones Indoleacetic acid Indolylacetic acid Larix sibirica Localization Phytohormones Pinus sibirica Plant hormones Somatic cells Somatic embryogenesis |
| title | The Role of Phytohormones in the Induction of Somatic Embryogenesis in Pinus sibirica and Larix sibirica |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T19%3A09%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Role%20of%20Phytohormones%20in%20the%20Induction%20of%20Somatic%20Embryogenesis%20in%20Pinus%20sibirica%20and%20Larix%20sibirica&rft.jtitle=CYTOLOGIA&rft.au=Tretyakova,%20Iraida%20N.&rft.date=2021-03-25&rft.volume=86&rft.issue=1&rft.spage=55&rft.epage=60&rft.pages=55-60&rft.issn=0011-4545&rft.eissn=1348-7019&rft_id=info:doi/10.1508/cytologia.86.55&rft_dat=%3Cproquest_cross%3E2507996556%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2507996556&rft_id=info:pmid/&rfr_iscdi=true |