Short Communication - The Inhibition of Cell Proliferation of Bile Duct Scar Fibroblasts in Miniature Pigs by 5-fluorouracil and its Mechanism
The purpose of this study was to investigate the effect of 5-fluorouracil (5-FU) on the expression of TGF-β1, cyclinD1, and CDK4 in fibroblasts of miniature pig bile duct scar model cultured in vitro. The bile duct scar fibroblasts were replicated by incision and anastomosis in miniature pigs and bi...
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| Veröffentlicht in: | Pakistan journal of zoology 2021-04, Vol.53 (2), p.765 |
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| creator | Deng, Shikang Jin, Yan Xu, Jing Zhu, Xiufang Hu, Pinghai Jiao, Li Zhang, Li Tang, Jianzhong |
| description | The purpose of this study was to investigate the effect of 5-fluorouracil (5-FU) on the expression of TGF-β1, cyclinD1, and CDK4 in fibroblasts of miniature pig bile duct scar model cultured in vitro. The bile duct scar fibroblasts were replicated by incision and anastomosis in miniature pigs and bile duct scar fibroblasts were obtained. After cell identification, the bile duct scar fibroblasts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibited, and the expressions of TGF-β1, cyclinD1, CDK4 mRNA and protein in the cells were down-regulated (P |
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| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2506714778</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A661699806</galeid><sourcerecordid>A661699806</sourcerecordid><originalsourceid>FETCH-LOGICAL-g958-65708cda2ddcee4da5c8f9920809667c167108d10019fd9698fc399890a245203</originalsourceid><addsrcrecordid>eNptUMtOwzAQzAEkqtJ_WIlzkJMmjn0sgUKlVlRq75Hj2M1Wjg12cuAn-GYsHoIDu4eVRjOzs3uRzAhZkpTzfHmVLEI4k1gFLfOczZL3Q-_8CLUbhsmiFCM6CykcewUb22OLn4DTUCtjYO-dQa28-EHv0Ci4n-QIByk8rLH1rjUijAHQwg4tinHyCvZ4CtC-QZlqMznvJi8kGhC2A4zcnZK9sBiG6-RSCxPU4nvOk-P64Vg_pdvnx0292qYnXrKUlhVhshN510mlik6Ukul4H2GEU1rJjFYZYV1GSMZ1xylnWi45Z5yIvChzspwnN1-2L969TiqMzTlGsnFjk5ckqouqYr-skzCqQavdGGMPGGSzojSj0ZHQyLr9hxW7UwNKZ5WOP_or-ACZbXql</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2506714778</pqid></control><display><type>article</type><title>Short Communication - The Inhibition of Cell Proliferation of Bile Duct Scar Fibroblasts in Miniature Pigs by 5-fluorouracil and its Mechanism</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Deng, Shikang ; Jin, Yan ; Xu, Jing ; Zhu, Xiufang ; Hu, Pinghai ; Jiao, Li ; Zhang, Li ; Tang, Jianzhong</creator><creatorcontrib>Deng, Shikang ; Jin, Yan ; Xu, Jing ; Zhu, Xiufang ; Hu, Pinghai ; Jiao, Li ; Zhang, Li ; Tang, Jianzhong</creatorcontrib><description>The purpose of this study was to investigate the effect of 5-fluorouracil (5-FU) on the expression of TGF-β1, cyclinD1, and CDK4 in fibroblasts of miniature pig bile duct scar model cultured in vitro. The bile duct scar fibroblasts were replicated by incision and anastomosis in miniature pigs and bile duct scar fibroblasts were obtained. After cell identification, the bile duct scar fibroblasts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibited, and the expressions of TGF-β1, cyclinD1, CDK4 mRNA and protein in the cells were down-regulated (P<0.05), showing concentration-dependence. In conclusion, 5-FU may have a role in inhibiting bile duct scars. One of the mechanisms may be related to its ability to inhibit the expression of TGF-β1, cyclinD1, and CDK4 in bile duct scar fibroblasts.</description><identifier>ISSN: 0030-9923</identifier><language>eng</language><publisher>Lahore: Knowledge Bylanes</publisher><subject>5-Fluorouracil ; Abdomen ; Anastomosis ; Bile ; Bile ducts ; Cell growth ; Cell interactions ; Cell proliferation ; Cholecystokinin ; Cyclin-dependent kinase 4 ; Experiments ; Fibroblasts ; Growth factors ; Hogs ; Hyperplasia ; mRNA ; Observations ; Physiological aspects ; Polymerase chain reaction ; Proteins ; Scars ; Software ; Swine ; Transforming growth factor-b1</subject><ispartof>Pakistan journal of zoology, 2021-04, Vol.53 (2), p.765</ispartof><rights>COPYRIGHT 2021 Knowledge Bylanes</rights><rights>(c)2021 Pakistan Journal of Zoology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Deng, Shikang</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Xu, Jing</creatorcontrib><creatorcontrib>Zhu, Xiufang</creatorcontrib><creatorcontrib>Hu, Pinghai</creatorcontrib><creatorcontrib>Jiao, Li</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Tang, Jianzhong</creatorcontrib><title>Short Communication - The Inhibition of Cell Proliferation of Bile Duct Scar Fibroblasts in Miniature Pigs by 5-fluorouracil and its Mechanism</title><title>Pakistan journal of zoology</title><description>The purpose of this study was to investigate the effect of 5-fluorouracil (5-FU) on the expression of TGF-β1, cyclinD1, and CDK4 in fibroblasts of miniature pig bile duct scar model cultured in vitro. The bile duct scar fibroblasts were replicated by incision and anastomosis in miniature pigs and bile duct scar fibroblasts were obtained. After cell identification, the bile duct scar fibroblasts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibited, and the expressions of TGF-β1, cyclinD1, CDK4 mRNA and protein in the cells were down-regulated (P<0.05), showing concentration-dependence. In conclusion, 5-FU may have a role in inhibiting bile duct scars. One of the mechanisms may be related to its ability to inhibit the expression of TGF-β1, cyclinD1, and CDK4 in bile duct scar fibroblasts.</description><subject>5-Fluorouracil</subject><subject>Abdomen</subject><subject>Anastomosis</subject><subject>Bile</subject><subject>Bile ducts</subject><subject>Cell growth</subject><subject>Cell interactions</subject><subject>Cell proliferation</subject><subject>Cholecystokinin</subject><subject>Cyclin-dependent kinase 4</subject><subject>Experiments</subject><subject>Fibroblasts</subject><subject>Growth factors</subject><subject>Hogs</subject><subject>Hyperplasia</subject><subject>mRNA</subject><subject>Observations</subject><subject>Physiological aspects</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Scars</subject><subject>Software</subject><subject>Swine</subject><subject>Transforming growth factor-b1</subject><issn>0030-9923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptUMtOwzAQzAEkqtJ_WIlzkJMmjn0sgUKlVlRq75Hj2M1Wjg12cuAn-GYsHoIDu4eVRjOzs3uRzAhZkpTzfHmVLEI4k1gFLfOczZL3Q-_8CLUbhsmiFCM6CykcewUb22OLn4DTUCtjYO-dQa28-EHv0Ci4n-QIByk8rLH1rjUijAHQwg4tinHyCvZ4CtC-QZlqMznvJi8kGhC2A4zcnZK9sBiG6-RSCxPU4nvOk-P64Vg_pdvnx0292qYnXrKUlhVhshN510mlik6Ukul4H2GEU1rJjFYZYV1GSMZ1xylnWi45Z5yIvChzspwnN1-2L969TiqMzTlGsnFjk5ckqouqYr-skzCqQavdGGMPGGSzojSj0ZHQyLr9hxW7UwNKZ5WOP_or-ACZbXql</recordid><startdate>20210430</startdate><enddate>20210430</enddate><creator>Deng, Shikang</creator><creator>Jin, Yan</creator><creator>Xu, Jing</creator><creator>Zhu, Xiufang</creator><creator>Hu, Pinghai</creator><creator>Jiao, Li</creator><creator>Zhang, Li</creator><creator>Tang, Jianzhong</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><scope>7QG</scope><scope>7SS</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20210430</creationdate><title>Short Communication - The Inhibition of Cell Proliferation of Bile Duct Scar Fibroblasts in Miniature Pigs by 5-fluorouracil and its Mechanism</title><author>Deng, Shikang ; Jin, Yan ; Xu, Jing ; Zhu, Xiufang ; Hu, Pinghai ; Jiao, Li ; Zhang, Li ; Tang, Jianzhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g958-65708cda2ddcee4da5c8f9920809667c167108d10019fd9698fc399890a245203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>5-Fluorouracil</topic><topic>Abdomen</topic><topic>Anastomosis</topic><topic>Bile</topic><topic>Bile ducts</topic><topic>Cell growth</topic><topic>Cell interactions</topic><topic>Cell proliferation</topic><topic>Cholecystokinin</topic><topic>Cyclin-dependent kinase 4</topic><topic>Experiments</topic><topic>Fibroblasts</topic><topic>Growth factors</topic><topic>Hogs</topic><topic>Hyperplasia</topic><topic>mRNA</topic><topic>Observations</topic><topic>Physiological aspects</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Scars</topic><topic>Software</topic><topic>Swine</topic><topic>Transforming growth factor-b1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Shikang</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><creatorcontrib>Xu, Jing</creatorcontrib><creatorcontrib>Zhu, Xiufang</creatorcontrib><creatorcontrib>Hu, Pinghai</creatorcontrib><creatorcontrib>Jiao, Li</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Tang, Jianzhong</creatorcontrib><collection>Animal Behavior Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Pakistan journal of zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Shikang</au><au>Jin, Yan</au><au>Xu, Jing</au><au>Zhu, Xiufang</au><au>Hu, Pinghai</au><au>Jiao, Li</au><au>Zhang, Li</au><au>Tang, Jianzhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short Communication - The Inhibition of Cell Proliferation of Bile Duct Scar Fibroblasts in Miniature Pigs by 5-fluorouracil and its Mechanism</atitle><jtitle>Pakistan journal of zoology</jtitle><date>2021-04-30</date><risdate>2021</risdate><volume>53</volume><issue>2</issue><spage>765</spage><pages>765-</pages><issn>0030-9923</issn><abstract>The purpose of this study was to investigate the effect of 5-fluorouracil (5-FU) on the expression of TGF-β1, cyclinD1, and CDK4 in fibroblasts of miniature pig bile duct scar model cultured in vitro. The bile duct scar fibroblasts were replicated by incision and anastomosis in miniature pigs and bile duct scar fibroblasts were obtained. After cell identification, the bile duct scar fibroblasts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibited, and the expressions of TGF-β1, cyclinD1, CDK4 mRNA and protein in the cells were down-regulated (P<0.05), showing concentration-dependence. In conclusion, 5-FU may have a role in inhibiting bile duct scars. One of the mechanisms may be related to its ability to inhibit the expression of TGF-β1, cyclinD1, and CDK4 in bile duct scar fibroblasts.</abstract><cop>Lahore</cop><pub>Knowledge Bylanes</pub></addata></record> |
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| subjects | 5-Fluorouracil Abdomen Anastomosis Bile Bile ducts Cell growth Cell interactions Cell proliferation Cholecystokinin Cyclin-dependent kinase 4 Experiments Fibroblasts Growth factors Hogs Hyperplasia mRNA Observations Physiological aspects Polymerase chain reaction Proteins Scars Software Swine Transforming growth factor-b1 |
| title | Short Communication - The Inhibition of Cell Proliferation of Bile Duct Scar Fibroblasts in Miniature Pigs by 5-fluorouracil and its Mechanism |
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