Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model
Introduction: Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment. This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic...
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| Veröffentlicht in: | Folia Medica 2020-12, Vol.62 (4), p.817-824 |
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| creator | Hosseinpour nader, Armin Sodagar, Ahmad Akhavan, Azam Pourhajibagher, Maryam Bahador, Abbas |
| description | Introduction:
Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment. This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.
Materials and methods:
Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared. The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs. The oral cavities of the rats were infected with cariogenic bacteria. After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds. Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer. Another drop (10 µL) of primer was applied and light-cured for 40 seconds. The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.
Results:
Adding 1% (p=0.005), 5% (p |
| doi_str_mv | 10.3897/folmed.62.e50200 |
| format | Article |
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Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment. This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.
Materials and methods:
Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared. The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs. The oral cavities of the rats were infected with cariogenic bacteria. After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds. Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer. Another drop (10 µL) of primer was applied and light-cured for 40 seconds. The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.
Results:
Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group. At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group. Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05). At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05). At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).
Conclusions:
The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.</description><identifier>ISSN: 0204-8043</identifier><identifier>EISSN: 1314-2143</identifier><identifier>DOI: 10.3897/folmed.62.e50200</identifier><language>eng</language><publisher>Plovdiv: MEDICAL UNIVERSITY- PLOVDIV</publisher><subject>Adhesives ; Bacteria ; Biofilms ; cariogenic bacteria ; chitosan ; composite ; Laboratory animals ; Microorganisms ; nanopart ; Nanoparticles ; Orthodontics ; Rodents ; Streptococcus infections</subject><ispartof>Folia Medica, 2020-12, Vol.62 (4), p.817-824</ispartof><rights>Copyright MEDICAL UNIVERSITY- PLOVDIV 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3270-fbc334936db05ca6265bfaea1fb1ddb116566e7344f150d0cbcda402877a3e053</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Hosseinpour nader, Armin</creatorcontrib><creatorcontrib>Sodagar, Ahmad</creatorcontrib><creatorcontrib>Akhavan, Azam</creatorcontrib><creatorcontrib>Pourhajibagher, Maryam</creatorcontrib><creatorcontrib>Bahador, Abbas</creatorcontrib><title>Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model</title><title>Folia Medica</title><description>Introduction:
Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment. This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.
Materials and methods:
Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared. The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs. The oral cavities of the rats were infected with cariogenic bacteria. After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds. Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer. Another drop (10 µL) of primer was applied and light-cured for 40 seconds. The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.
Results:
Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group. At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group. Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05). At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05). At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).
Conclusions:
The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.</description><subject>Adhesives</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>cariogenic bacteria</subject><subject>chitosan</subject><subject>composite</subject><subject>Laboratory animals</subject><subject>Microorganisms</subject><subject>nanopart</subject><subject>Nanoparticles</subject><subject>Orthodontics</subject><subject>Rodents</subject><subject>Streptococcus infections</subject><issn>0204-8043</issn><issn>1314-2143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DOA</sourceid><recordid>eNo9kUtv2zAQhImiBeKmvedIoGc5y6fkY2KkSYA8iqI9Eys-bBqy6JL0Ib-jfzhyZfS0wO7imxkMIVcMlqJbtdchDXvvlpovvQIO8IEsmGCy4UyKj2QxrWTTgRQX5HMpOwCtANSC_L0Za-zRVp8jDvQuBG9roSnQ11y3yaXpbOmPHPc-0wfMfcpx3ND1NtZUcKQvOKYD5ulp8IXiBuNYKq1bT5-PQ43l4G2cDrcxhTjsT9w15pg2fpywt2ddGkeK9CdW-pycH76QTwGH4r-e5yX5_f3u1_qheXq9f1zfPDVW8Baa0Fsh5Epo14OyqLlWfUCPLPTMuZ4xrbT2rZAyMAUObG8dSuBd26LwoMQleZy5LuHOHKaMmN9Mwmj-LVLemHMyw1odVgJRuzZI3ttVx3THbesYxw4ETqxvM-uQ05-jL9Xs0jGPk33DFcjJiZQnRZi_bE6lZB_-qzIwpxrNXKPR3Mw1inceDpO-</recordid><startdate>20201231</startdate><enddate>20201231</enddate><creator>Hosseinpour nader, Armin</creator><creator>Sodagar, Ahmad</creator><creator>Akhavan, Azam</creator><creator>Pourhajibagher, Maryam</creator><creator>Bahador, Abbas</creator><general>MEDICAL UNIVERSITY- PLOVDIV</general><general>Pensoft Publishers</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20201231</creationdate><title>Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model</title><author>Hosseinpour nader, Armin ; Sodagar, Ahmad ; Akhavan, Azam ; Pourhajibagher, Maryam ; Bahador, Abbas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3270-fbc334936db05ca6265bfaea1fb1ddb116566e7344f150d0cbcda402877a3e053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adhesives</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>cariogenic bacteria</topic><topic>chitosan</topic><topic>composite</topic><topic>Laboratory animals</topic><topic>Microorganisms</topic><topic>nanopart</topic><topic>Nanoparticles</topic><topic>Orthodontics</topic><topic>Rodents</topic><topic>Streptococcus infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hosseinpour nader, Armin</creatorcontrib><creatorcontrib>Sodagar, Ahmad</creatorcontrib><creatorcontrib>Akhavan, Azam</creatorcontrib><creatorcontrib>Pourhajibagher, Maryam</creatorcontrib><creatorcontrib>Bahador, Abbas</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Folia Medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hosseinpour nader, Armin</au><au>Sodagar, Ahmad</au><au>Akhavan, Azam</au><au>Pourhajibagher, Maryam</au><au>Bahador, Abbas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model</atitle><jtitle>Folia Medica</jtitle><date>2020-12-31</date><risdate>2020</risdate><volume>62</volume><issue>4</issue><spage>817</spage><epage>824</epage><pages>817-824</pages><issn>0204-8043</issn><eissn>1314-2143</eissn><abstract>Introduction:
Microbial biofilm accumulation around orthodontic brackets and composite is a common complication of fixed orth-odontic treatment. This study assessed the antibacterial effects of orthodontic primer containing chitosan nanoparticles (CNPs) against the multispecies biofilm of cariogenic bacteria in а rat model.
Materials and methods:
Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% CNPs was experimentally prepared. The Wistar rats were randomly divided into four groups (n=7) of control (0% CNPs), 1%, 5% and 10% CNPs. The oral cavities of the rats were infected with cariogenic bacteria. After anesthetizing the rats, 1 drop (10 µL) of primer with different concentrations of CNPs was applied to their central incisor and light-cured for 20 seconds. Transbond XT orthodontic adhesive (2 × 2 mm) was applied on the primer. Another drop (10 µL) of primer was applied and light-cured for 40 seconds. The number of Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus colonies in the saliva of rats was quantified at 24 hours, 4 days and 7 days.
Results:
Adding 1% (p=0.005), 5% (p<0.001) and 10% (p<0.001) of CNPs to orthodontic primer significantly reduced the S. mutans colony count at 24 hours compared with the control group. At 24 hours, the mean S. sanguinis colony counts in the 5% (p=0.04) and 10% (p=0.02) CNP groups were significantly lower than that in the control group. Also, at 4 and 7 days, the mean colony counts in the 5% and 10% CNP groups were significantly lower than that in the control group (p<0.05). At 24 hours and 4 days, the mean L. acidophilus colony count in the 10% CNP group was significantly lower than that in the control group (p<0.05). At 7 days, rats with failed adhesive showed a significantly higher count of all three bacteria compared with rats with adhesive (p<0.05).
Conclusions:
The addition of 5% CNPs to orthodontic primer significantly decreased the colony count of cariogenic bacteria in rats.</abstract><cop>Plovdiv</cop><pub>MEDICAL UNIVERSITY- PLOVDIV</pub><doi>10.3897/folmed.62.e50200</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesives Bacteria Biofilms cariogenic bacteria chitosan composite Laboratory animals Microorganisms nanopart Nanoparticles Orthodontics Rodents Streptococcus infections |
| title | Antibacterial Effects of Orthodontic Primer Harboring Chitosan Nanoparticles against the Multispecies Biofilm of Cariogenic Bacteria in a Rat Model |
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