Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment
We describe a method for experimental assessment of endothelial dysfunction in an isolated using extracorporeal machine perfusion and vasoactive substrates agents (norepinephrine and acetylcholine). Quantitative assessment of endothelial vasomotor dysfunction was proposed, based on registration of c...
Gespeichert in:
| Veröffentlicht in: | Bulletin of experimental biology and medicine 2021-02, Vol.170 (4), p.511-514 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 514 |
|---|---|
| container_issue | 4 |
| container_start_page | 511 |
| container_title | Bulletin of experimental biology and medicine |
| container_volume | 170 |
| creator | Ermolaev, P. A. Khramykh, T. P. Korpacheva, O. V. Barskaya, L. O. Zolotov, A. N. |
| description | We describe a method for experimental assessment of endothelial dysfunction in an isolated using extracorporeal machine perfusion and vasoactive substrates agents (norepinephrine and acetylcholine). Quantitative assessment of endothelial vasomotor dysfunction was proposed, based on registration of changes in volumetric perfusate flow rate through the isolated vessel. Functional activity of the isolated aorta endothelium and total content of stable NO degradation products in blood plasma were studied in outbred white male rats after subtotal liver resection. Impaired vasodilation ability of the aorta and increased content of NO degradation products in blood plasma observed after surgery indicate the development of vasomotor endothelial dysfunction associated with oxidative stress, cholemia, and endotoxemia. |
| doi_str_mv | 10.1007/s10517-021-05098-3 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2503525221</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A665614810</galeid><sourcerecordid>A665614810</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-4b99f22f58138d4552c8e0ebbcec8a3777ddeb02d6c8798474246b874b1e9f203</originalsourceid><addsrcrecordid>eNqNkl1rFDEUhgdR7Fr9A17IgCCCTM3H5GMvy7LVQqU32ktDJnNmNyWbrElG7b9vplNbKyKSi3zwvCc575uqeonREUZIvE8YMSwaRHCDGFrKhj6qFpgJ2khC8ONqgQrVtFLKg-pZSpfTFnH8tDqgVGBKKF1UX8-j3VivXf0J8jb09RBivf6u3aiz9Zt67fuQt-BsIS50CruQC3AyepNt8HUY6tMUnM7Q1xeQErja-nr9cw_R7sDn59WTQbsEL27nw-rLyfrz6mNzdv7hdHV81piWtLlpu-VyIGRgElPZt4wRIwFB1xkwUlMhRN9Dh0jPjRRL2Yqi4p0UbYehCBE9rN7OdfcxfBshZbWzyYBz2kMYkyIMYcI5lrSgr_9AL8MYiwM3FGWEFfPuqY12oKwfQo7aTEXVMeeM41bi6dqjv1Bl9LCzJngYbDl_IHjzm2AL2uVt8W-czEwPQTKDJoaUIgxqXxzV8UphpKb01Zy-Kumrm_TV1Nqr29bGbgf9neRX3AV4NwM_oAtDMha8gTus_A9OZCspLys00fL_6ZXNeupiFUafi5TO0lRwv4F4b_I_3n8NJrnXzg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2503525221</pqid></control><display><type>article</type><title>Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment</title><source>SpringerNature Journals</source><creator>Ermolaev, P. A. ; Khramykh, T. P. ; Korpacheva, O. V. ; Barskaya, L. O. ; Zolotov, A. N.</creator><creatorcontrib>Ermolaev, P. A. ; Khramykh, T. P. ; Korpacheva, O. V. ; Barskaya, L. O. ; Zolotov, A. N.</creatorcontrib><description>We describe a method for experimental assessment of endothelial dysfunction in an isolated using extracorporeal machine perfusion and vasoactive substrates agents (norepinephrine and acetylcholine). Quantitative assessment of endothelial vasomotor dysfunction was proposed, based on registration of changes in volumetric perfusate flow rate through the isolated vessel. Functional activity of the isolated aorta endothelium and total content of stable NO degradation products in blood plasma were studied in outbred white male rats after subtotal liver resection. Impaired vasodilation ability of the aorta and increased content of NO degradation products in blood plasma observed after surgery indicate the development of vasomotor endothelial dysfunction associated with oxidative stress, cholemia, and endotoxemia.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-021-05098-3</identifier><identifier>PMID: 33713233</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acetylcholine ; Aorta ; Biomedical and Life Sciences ; Biomedicine ; Blood vessels ; Cell Biology ; Coronary vessels ; Degradation products ; Dilatation ; Endothelium ; Endotoxemia ; Internal Medicine ; Laboratory Medicine ; Life Sciences & Biomedicine ; Liver ; Medicine, Research & Experimental ; Methods ; Norepinephrine ; Oxidative stress ; Pathology ; Perfusion ; Research & Experimental Medicine ; Science & Technology ; Surgery ; Vasoactive agents ; Vasodilation</subject><ispartof>Bulletin of experimental biology and medicine, 2021-02, Vol.170 (4), p.511-514</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>0</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000628483600003</woscitedreferencesoriginalsourcerecordid><cites>FETCH-LOGICAL-c424t-4b99f22f58138d4552c8e0ebbcec8a3777ddeb02d6c8798474246b874b1e9f203</cites><orcidid>0000-0001-6110-3933 ; 0000-0002-6775-323X ; 0000-0002-5508-6679 ; 0000-0001-8733-6102</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-021-05098-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-021-05098-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33713233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ermolaev, P. A.</creatorcontrib><creatorcontrib>Khramykh, T. P.</creatorcontrib><creatorcontrib>Korpacheva, O. V.</creatorcontrib><creatorcontrib>Barskaya, L. O.</creatorcontrib><creatorcontrib>Zolotov, A. N.</creatorcontrib><title>Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>B EXP BIOL MED</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We describe a method for experimental assessment of endothelial dysfunction in an isolated using extracorporeal machine perfusion and vasoactive substrates agents (norepinephrine and acetylcholine). Quantitative assessment of endothelial vasomotor dysfunction was proposed, based on registration of changes in volumetric perfusate flow rate through the isolated vessel. Functional activity of the isolated aorta endothelium and total content of stable NO degradation products in blood plasma were studied in outbred white male rats after subtotal liver resection. Impaired vasodilation ability of the aorta and increased content of NO degradation products in blood plasma observed after surgery indicate the development of vasomotor endothelial dysfunction associated with oxidative stress, cholemia, and endotoxemia.</description><subject>Acetylcholine</subject><subject>Aorta</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood vessels</subject><subject>Cell Biology</subject><subject>Coronary vessels</subject><subject>Degradation products</subject><subject>Dilatation</subject><subject>Endothelium</subject><subject>Endotoxemia</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Life Sciences & Biomedicine</subject><subject>Liver</subject><subject>Medicine, Research & Experimental</subject><subject>Methods</subject><subject>Norepinephrine</subject><subject>Oxidative stress</subject><subject>Pathology</subject><subject>Perfusion</subject><subject>Research & Experimental Medicine</subject><subject>Science & Technology</subject><subject>Surgery</subject><subject>Vasoactive agents</subject><subject>Vasodilation</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl1rFDEUhgdR7Fr9A17IgCCCTM3H5GMvy7LVQqU32ktDJnNmNyWbrElG7b9vplNbKyKSi3zwvCc575uqeonREUZIvE8YMSwaRHCDGFrKhj6qFpgJ2khC8ONqgQrVtFLKg-pZSpfTFnH8tDqgVGBKKF1UX8-j3VivXf0J8jb09RBivf6u3aiz9Zt67fuQt-BsIS50CruQC3AyepNt8HUY6tMUnM7Q1xeQErja-nr9cw_R7sDn59WTQbsEL27nw-rLyfrz6mNzdv7hdHV81piWtLlpu-VyIGRgElPZt4wRIwFB1xkwUlMhRN9Dh0jPjRRL2Yqi4p0UbYehCBE9rN7OdfcxfBshZbWzyYBz2kMYkyIMYcI5lrSgr_9AL8MYiwM3FGWEFfPuqY12oKwfQo7aTEXVMeeM41bi6dqjv1Bl9LCzJngYbDl_IHjzm2AL2uVt8W-czEwPQTKDJoaUIgxqXxzV8UphpKb01Zy-Kumrm_TV1Nqr29bGbgf9neRX3AV4NwM_oAtDMha8gTus_A9OZCspLys00fL_6ZXNeupiFUafi5TO0lRwv4F4b_I_3n8NJrnXzg</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Ermolaev, P. A.</creator><creator>Khramykh, T. P.</creator><creator>Korpacheva, O. V.</creator><creator>Barskaya, L. O.</creator><creator>Zolotov, A. N.</creator><general>Springer US</general><general>Springer Nature</general><general>Springer</general><general>Springer Nature B.V</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6110-3933</orcidid><orcidid>https://orcid.org/0000-0002-6775-323X</orcidid><orcidid>https://orcid.org/0000-0002-5508-6679</orcidid><orcidid>https://orcid.org/0000-0001-8733-6102</orcidid></search><sort><creationdate>20210201</creationdate><title>Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment</title><author>Ermolaev, P. A. ; Khramykh, T. P. ; Korpacheva, O. V. ; Barskaya, L. O. ; Zolotov, A. N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-4b99f22f58138d4552c8e0ebbcec8a3777ddeb02d6c8798474246b874b1e9f203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acetylcholine</topic><topic>Aorta</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood vessels</topic><topic>Cell Biology</topic><topic>Coronary vessels</topic><topic>Degradation products</topic><topic>Dilatation</topic><topic>Endothelium</topic><topic>Endotoxemia</topic><topic>Internal Medicine</topic><topic>Laboratory Medicine</topic><topic>Life Sciences & Biomedicine</topic><topic>Liver</topic><topic>Medicine, Research & Experimental</topic><topic>Methods</topic><topic>Norepinephrine</topic><topic>Oxidative stress</topic><topic>Pathology</topic><topic>Perfusion</topic><topic>Research & Experimental Medicine</topic><topic>Science & Technology</topic><topic>Surgery</topic><topic>Vasoactive agents</topic><topic>Vasodilation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ermolaev, P. A.</creatorcontrib><creatorcontrib>Khramykh, T. P.</creatorcontrib><creatorcontrib>Korpacheva, O. V.</creatorcontrib><creatorcontrib>Barskaya, L. O.</creatorcontrib><creatorcontrib>Zolotov, A. N.</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Bulletin of experimental biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ermolaev, P. A.</au><au>Khramykh, T. P.</au><au>Korpacheva, O. V.</au><au>Barskaya, L. O.</au><au>Zolotov, A. N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><stitle>B EXP BIOL MED</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>170</volume><issue>4</issue><spage>511</spage><epage>514</epage><pages>511-514</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>We describe a method for experimental assessment of endothelial dysfunction in an isolated using extracorporeal machine perfusion and vasoactive substrates agents (norepinephrine and acetylcholine). Quantitative assessment of endothelial vasomotor dysfunction was proposed, based on registration of changes in volumetric perfusate flow rate through the isolated vessel. Functional activity of the isolated aorta endothelium and total content of stable NO degradation products in blood plasma were studied in outbred white male rats after subtotal liver resection. Impaired vasodilation ability of the aorta and increased content of NO degradation products in blood plasma observed after surgery indicate the development of vasomotor endothelial dysfunction associated with oxidative stress, cholemia, and endotoxemia.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33713233</pmid><doi>10.1007/s10517-021-05098-3</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0001-6110-3933</orcidid><orcidid>https://orcid.org/0000-0002-6775-323X</orcidid><orcidid>https://orcid.org/0000-0002-5508-6679</orcidid><orcidid>https://orcid.org/0000-0001-8733-6102</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-4888 |
| ispartof | Bulletin of experimental biology and medicine, 2021-02, Vol.170 (4), p.511-514 |
| issn | 0007-4888 1573-8221 |
| language | eng |
| recordid | cdi_proquest_journals_2503525221 |
| source | SpringerNature Journals |
| subjects | Acetylcholine Aorta Biomedical and Life Sciences Biomedicine Blood vessels Cell Biology Coronary vessels Degradation products Dilatation Endothelium Endotoxemia Internal Medicine Laboratory Medicine Life Sciences & Biomedicine Liver Medicine, Research & Experimental Methods Norepinephrine Oxidative stress Pathology Perfusion Research & Experimental Medicine Science & Technology Surgery Vasoactive agents Vasodilation |
| title | Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T20%3A13%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Original%20Method%20for%20Evaluating%20Endothelial%20Vasomotor%20Function%20of%20Isolated%20Vessel%20in%20Experiment&rft.jtitle=Bulletin%20of%20experimental%20biology%20and%20medicine&rft.au=Ermolaev,%20P.%20A.&rft.date=2021-02-01&rft.volume=170&rft.issue=4&rft.spage=511&rft.epage=514&rft.pages=511-514&rft.issn=0007-4888&rft.eissn=1573-8221&rft_id=info:doi/10.1007/s10517-021-05098-3&rft_dat=%3Cgale_proqu%3EA665614810%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2503525221&rft_id=info:pmid/33713233&rft_galeid=A665614810&rfr_iscdi=true |