Development of electrochemical aptasensor based on gold nanoparticles and electrospun carbon nanofibers for the detection of aflatoxin M1 in milk
An electrochemical aptasensor was developed based on electrospun carbon nanofiber (ECNF) mat for detection of aflatoxin M1 (AFM1) in the milk samples. The ECNF mat was firstly produced by electrospinning and heat treatment method utilized directly as a novel substrate electrode. This electrode was t...
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| Veröffentlicht in: | Journal of food measurement & characterization 2021-04, Vol.15 (2), p.1826-1833 |
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| creator | Rahmani, Hamid Reza Adabi, Mohsen Bagheri, Kamran Pooshang Karim, Giti |
| description | An electrochemical aptasensor was developed based on electrospun carbon nanofiber (ECNF) mat for detection of aflatoxin M1 (AFM1) in the milk samples. The ECNF mat was firstly produced by electrospinning and heat treatment method utilized directly as a novel substrate electrode. This electrode was then modified by electrodeposition of gold nanoparticles (AuNPs) and immobilization of a thiol-modified single stranded DNA (ss-HSDNA), respectively. The structure and morphology of prepared ECNF mat and AuNPs/ECNF mat electrode were characterized by means of Raman spectroscopy and scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The characterization results indicated that the gold nanopartciles were uniformly electrodeposited on ECNF mat electrode. To analysis each step of the aptasensor development, cyclic voltammetry (CV) experiments were carried out in the [Fe(CN)
6
]
−3/−4
solution. The biosensor exhibited a low detection limit (0.6 pg/mL), a wide and useful linear range (1-100 pg/mL), high sensitivity, excellent stability and reproducibility as well as good recovery. Moreover, the results obtained in this research were comparable to those obtained using the HPLC. Thus, good electrochemical performance and simple preparation procedure of the ss-HSDNA/AuNPs/ECNF mat electrode can provide promising potential for the detection of AFM1 in milk samples. |
| doi_str_mv | 10.1007/s11694-020-00780-y |
| format | Article |
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6
]
−3/−4
solution. The biosensor exhibited a low detection limit (0.6 pg/mL), a wide and useful linear range (1-100 pg/mL), high sensitivity, excellent stability and reproducibility as well as good recovery. Moreover, the results obtained in this research were comparable to those obtained using the HPLC. Thus, good electrochemical performance and simple preparation procedure of the ss-HSDNA/AuNPs/ECNF mat electrode can provide promising potential for the detection of AFM1 in milk samples.</description><identifier>ISSN: 2193-4126</identifier><identifier>EISSN: 2193-4134</identifier><identifier>DOI: 10.1007/s11694-020-00780-y</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aflatoxin M1 ; Aflatoxins ; Biosensors ; Carbon fibers ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; DNA structure ; Electrochemical analysis ; Electrochemistry ; Electrodes ; Electrospinning ; Engineering ; Food Science ; Gold ; Heat treatment ; Heat treatments ; High-performance liquid chromatography ; Immobilization ; Liquid chromatography ; Milk ; Morphology ; Nanofibers ; Nanoparticles ; Original Paper ; Raman spectroscopy ; Scanning electron microscopy ; Spectroscopy ; Spectrum analysis ; Substrates</subject><ispartof>Journal of food measurement & characterization, 2021-04, Vol.15 (2), p.1826-1833</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-3e385431c7f1ea64c9047e6574a0e6b18528bcbc53496bfb3877e93d086dd3253</citedby><cites>FETCH-LOGICAL-c319t-3e385431c7f1ea64c9047e6574a0e6b18528bcbc53496bfb3877e93d086dd3253</cites><orcidid>0000-0001-7985-9114</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11694-020-00780-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11694-020-00780-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Rahmani, Hamid Reza</creatorcontrib><creatorcontrib>Adabi, Mohsen</creatorcontrib><creatorcontrib>Bagheri, Kamran Pooshang</creatorcontrib><creatorcontrib>Karim, Giti</creatorcontrib><title>Development of electrochemical aptasensor based on gold nanoparticles and electrospun carbon nanofibers for the detection of aflatoxin M1 in milk</title><title>Journal of food measurement & characterization</title><addtitle>Food Measure</addtitle><description>An electrochemical aptasensor was developed based on electrospun carbon nanofiber (ECNF) mat for detection of aflatoxin M1 (AFM1) in the milk samples. The ECNF mat was firstly produced by electrospinning and heat treatment method utilized directly as a novel substrate electrode. This electrode was then modified by electrodeposition of gold nanoparticles (AuNPs) and immobilization of a thiol-modified single stranded DNA (ss-HSDNA), respectively. The structure and morphology of prepared ECNF mat and AuNPs/ECNF mat electrode were characterized by means of Raman spectroscopy and scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The characterization results indicated that the gold nanopartciles were uniformly electrodeposited on ECNF mat electrode. To analysis each step of the aptasensor development, cyclic voltammetry (CV) experiments were carried out in the [Fe(CN)
6
]
−3/−4
solution. The biosensor exhibited a low detection limit (0.6 pg/mL), a wide and useful linear range (1-100 pg/mL), high sensitivity, excellent stability and reproducibility as well as good recovery. Moreover, the results obtained in this research were comparable to those obtained using the HPLC. Thus, good electrochemical performance and simple preparation procedure of the ss-HSDNA/AuNPs/ECNF mat electrode can provide promising potential for the detection of AFM1 in milk samples.</description><subject>Aflatoxin M1</subject><subject>Aflatoxins</subject><subject>Biosensors</subject><subject>Carbon fibers</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>DNA structure</subject><subject>Electrochemical analysis</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Electrospinning</subject><subject>Engineering</subject><subject>Food Science</subject><subject>Gold</subject><subject>Heat treatment</subject><subject>Heat treatments</subject><subject>High-performance liquid chromatography</subject><subject>Immobilization</subject><subject>Liquid chromatography</subject><subject>Milk</subject><subject>Morphology</subject><subject>Nanofibers</subject><subject>Nanoparticles</subject><subject>Original Paper</subject><subject>Raman spectroscopy</subject><subject>Scanning electron microscopy</subject><subject>Spectroscopy</subject><subject>Spectrum analysis</subject><subject>Substrates</subject><issn>2193-4126</issn><issn>2193-4134</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kEtPAyEUhYnRxKb2D7gicT0KA_NamvpMatzomjDMpZ1KYQRq7M_wH0sdHzs3cG_4zjnhIHRKyTklpLoIlJYNz0hOsrTWJNsdoElOG5Zxyvjh75yXx2gWwpoQQmnFeckm6OMK3sC4YQM2YqcxGFDRO7WCTa-kwXKIMoANzuM2DR12Fi-d6bCV1g3Sx14ZCFja7kcahq3FSvo2kXtI9y34gHVyiCvAHcSE9ekxpUltZHTvvcUPFKdz05uXE3SkpQkw-76n6Pnm-ml-ly0eb-_nl4tMMdrEjAGrC86oqjQFWXLVEF5BWVRcEihbWhd53apWFYw3ZatbVlcVNKwjddl1LC_YFJ2NvoN3r1sIUazd1tsUKfKCpIyaMZqofKRU-lnwoMXg-430O0GJ2LcvxvZFal98tS92ScRGUUiwXYL_s_5H9QmM6Ip6</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Rahmani, Hamid Reza</creator><creator>Adabi, Mohsen</creator><creator>Bagheri, Kamran Pooshang</creator><creator>Karim, Giti</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><orcidid>https://orcid.org/0000-0001-7985-9114</orcidid></search><sort><creationdate>20210401</creationdate><title>Development of electrochemical aptasensor based on gold nanoparticles and electrospun carbon nanofibers for the detection of aflatoxin M1 in milk</title><author>Rahmani, Hamid Reza ; Adabi, Mohsen ; Bagheri, Kamran Pooshang ; Karim, Giti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-3e385431c7f1ea64c9047e6574a0e6b18528bcbc53496bfb3877e93d086dd3253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aflatoxin M1</topic><topic>Aflatoxins</topic><topic>Biosensors</topic><topic>Carbon fibers</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>DNA structure</topic><topic>Electrochemical analysis</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Electrospinning</topic><topic>Engineering</topic><topic>Food Science</topic><topic>Gold</topic><topic>Heat treatment</topic><topic>Heat treatments</topic><topic>High-performance liquid chromatography</topic><topic>Immobilization</topic><topic>Liquid chromatography</topic><topic>Milk</topic><topic>Morphology</topic><topic>Nanofibers</topic><topic>Nanoparticles</topic><topic>Original Paper</topic><topic>Raman spectroscopy</topic><topic>Scanning electron microscopy</topic><topic>Spectroscopy</topic><topic>Spectrum analysis</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahmani, Hamid Reza</creatorcontrib><creatorcontrib>Adabi, Mohsen</creatorcontrib><creatorcontrib>Bagheri, Kamran Pooshang</creatorcontrib><creatorcontrib>Karim, Giti</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><jtitle>Journal of food measurement & characterization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahmani, Hamid Reza</au><au>Adabi, Mohsen</au><au>Bagheri, Kamran Pooshang</au><au>Karim, Giti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of electrochemical aptasensor based on gold nanoparticles and electrospun carbon nanofibers for the detection of aflatoxin M1 in milk</atitle><jtitle>Journal of food measurement & characterization</jtitle><stitle>Food Measure</stitle><date>2021-04-01</date><risdate>2021</risdate><volume>15</volume><issue>2</issue><spage>1826</spage><epage>1833</epage><pages>1826-1833</pages><issn>2193-4126</issn><eissn>2193-4134</eissn><abstract>An electrochemical aptasensor was developed based on electrospun carbon nanofiber (ECNF) mat for detection of aflatoxin M1 (AFM1) in the milk samples. The ECNF mat was firstly produced by electrospinning and heat treatment method utilized directly as a novel substrate electrode. This electrode was then modified by electrodeposition of gold nanoparticles (AuNPs) and immobilization of a thiol-modified single stranded DNA (ss-HSDNA), respectively. The structure and morphology of prepared ECNF mat and AuNPs/ECNF mat electrode were characterized by means of Raman spectroscopy and scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The characterization results indicated that the gold nanopartciles were uniformly electrodeposited on ECNF mat electrode. To analysis each step of the aptasensor development, cyclic voltammetry (CV) experiments were carried out in the [Fe(CN)
6
]
−3/−4
solution. The biosensor exhibited a low detection limit (0.6 pg/mL), a wide and useful linear range (1-100 pg/mL), high sensitivity, excellent stability and reproducibility as well as good recovery. Moreover, the results obtained in this research were comparable to those obtained using the HPLC. Thus, good electrochemical performance and simple preparation procedure of the ss-HSDNA/AuNPs/ECNF mat electrode can provide promising potential for the detection of AFM1 in milk samples.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11694-020-00780-y</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7985-9114</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aflatoxin M1 Aflatoxins Biosensors Carbon fibers Chemistry Chemistry and Materials Science Chemistry/Food Science DNA structure Electrochemical analysis Electrochemistry Electrodes Electrospinning Engineering Food Science Gold Heat treatment Heat treatments High-performance liquid chromatography Immobilization Liquid chromatography Milk Morphology Nanofibers Nanoparticles Original Paper Raman spectroscopy Scanning electron microscopy Spectroscopy Spectrum analysis Substrates |
| title | Development of electrochemical aptasensor based on gold nanoparticles and electrospun carbon nanofibers for the detection of aflatoxin M1 in milk |
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