Determination of Macrolide Antimicrobials in Infant Formulas Using a Modified Alkaline QuEChERS and High-performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
Macrolides are antimicrobials widely used in dairy farming, classified as critically important for human and animal health, and a priority for actions against antimicrobial resistance. Infant formula is used as a substitute for breast milk and should be safe to support child growth and development....
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| Veröffentlicht in: | Food analytical methods 2021-04, Vol.14 (4), p.719-733 |
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| creator | de Mendonça Pereira, Bianca Figueiredo Pereira, Mararlene Ulberg Ferreira, Rosana Gomes Spisso, Bernardete Ferraz |
| description | Macrolides are antimicrobials widely used in dairy farming, classified as critically important for human and animal health, and a priority for actions against antimicrobial resistance. Infant formula is used as a substitute for breast milk and should be safe to support child growth and development. In this context, the aim of this study was to validate an analytical method for macrolide analysis in infant formulas produced from bovine milk. The fast and practical method, based on a modified QuEChERS alkaline extraction and liquid chromatography-tandem mass spectrometry, was validated according to European Community guidelines and showed to be quantitative for erythromycin, spiramycin, oleandomycin, and tylosin, while qualitative for tilmicosin. Limits of detection (LODs) for ready-to-eat infant formulas ranged from 0.04 to 0.37 ng mL
−1
and limits of quantification (LOQs) from 0.15 to 1.22 ng mL
−1
. LODs were sufficiently low to detect residues of at least one of the assessed antimicrobials in 76.7% of the samples analyzed by the validated method. |
| doi_str_mv | 10.1007/s12161-020-01905-1 |
| format | Article |
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−1
and limits of quantification (LOQs) from 0.15 to 1.22 ng mL
−1
. LODs were sufficiently low to detect residues of at least one of the assessed antimicrobials in 76.7% of the samples analyzed by the validated method.</description><identifier>ISSN: 1936-9751</identifier><identifier>EISSN: 1936-976X</identifier><identifier>DOI: 10.1007/s12161-020-01905-1</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Animal health ; Antimicrobial agents ; Antimicrobial resistance ; Baby foods ; Breast milk ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Chromatography ; Cow's milk ; Dairy farms ; Erythromycin ; Food Science ; High performance liquid chromatography ; Infant formulas ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Microbiology ; Milk ; Oleandomycin ; Scientific imaging ; Spectroscopy ; Spiramycin ; Tilmicosin ; Tylosin</subject><ispartof>Food analytical methods, 2021-04, Vol.14 (4), p.719-733</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-606d11089e8ad6cabbac4ba92421cbd8a14ccf9a470f1ecea3f2c770a59765723</citedby><cites>FETCH-LOGICAL-c319t-606d11089e8ad6cabbac4ba92421cbd8a14ccf9a470f1ecea3f2c770a59765723</cites><orcidid>0000-0002-6753-1258</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12161-020-01905-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12161-020-01905-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>de Mendonça Pereira, Bianca Figueiredo</creatorcontrib><creatorcontrib>Pereira, Mararlene Ulberg</creatorcontrib><creatorcontrib>Ferreira, Rosana Gomes</creatorcontrib><creatorcontrib>Spisso, Bernardete Ferraz</creatorcontrib><title>Determination of Macrolide Antimicrobials in Infant Formulas Using a Modified Alkaline QuEChERS and High-performance Liquid Chromatography Coupled to Tandem Mass Spectrometry</title><title>Food analytical methods</title><addtitle>Food Anal. Methods</addtitle><description>Macrolides are antimicrobials widely used in dairy farming, classified as critically important for human and animal health, and a priority for actions against antimicrobial resistance. Infant formula is used as a substitute for breast milk and should be safe to support child growth and development. In this context, the aim of this study was to validate an analytical method for macrolide analysis in infant formulas produced from bovine milk. The fast and practical method, based on a modified QuEChERS alkaline extraction and liquid chromatography-tandem mass spectrometry, was validated according to European Community guidelines and showed to be quantitative for erythromycin, spiramycin, oleandomycin, and tylosin, while qualitative for tilmicosin. Limits of detection (LODs) for ready-to-eat infant formulas ranged from 0.04 to 0.37 ng mL
−1
and limits of quantification (LOQs) from 0.15 to 1.22 ng mL
−1
. 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Methods</stitle><date>2021-04-01</date><risdate>2021</risdate><volume>14</volume><issue>4</issue><spage>719</spage><epage>733</epage><pages>719-733</pages><issn>1936-9751</issn><eissn>1936-976X</eissn><abstract>Macrolides are antimicrobials widely used in dairy farming, classified as critically important for human and animal health, and a priority for actions against antimicrobial resistance. Infant formula is used as a substitute for breast milk and should be safe to support child growth and development. In this context, the aim of this study was to validate an analytical method for macrolide analysis in infant formulas produced from bovine milk. The fast and practical method, based on a modified QuEChERS alkaline extraction and liquid chromatography-tandem mass spectrometry, was validated according to European Community guidelines and showed to be quantitative for erythromycin, spiramycin, oleandomycin, and tylosin, while qualitative for tilmicosin. Limits of detection (LODs) for ready-to-eat infant formulas ranged from 0.04 to 0.37 ng mL
−1
and limits of quantification (LOQs) from 0.15 to 1.22 ng mL
−1
. LODs were sufficiently low to detect residues of at least one of the assessed antimicrobials in 76.7% of the samples analyzed by the validated method.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12161-020-01905-1</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-6753-1258</orcidid></addata></record> |
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| subjects | Analytical Chemistry Animal health Antimicrobial agents Antimicrobial resistance Baby foods Breast milk Chemistry Chemistry and Materials Science Chemistry/Food Science Chromatography Cow's milk Dairy farms Erythromycin Food Science High performance liquid chromatography Infant formulas Liquid chromatography Mass spectrometry Mass spectroscopy Microbiology Milk Oleandomycin Scientific imaging Spectroscopy Spiramycin Tilmicosin Tylosin |
| title | Determination of Macrolide Antimicrobials in Infant Formulas Using a Modified Alkaline QuEChERS and High-performance Liquid Chromatography Coupled to Tandem Mass Spectrometry |
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