In Vitro Antiplasmodial Activity of an Azadirachta indica Cell Culture Extract
Azadirachta indica A. Juss (neem tree) is used in Nigeria and India to control malaria. Due to the difficulty to obtain secondary metabolites from this plant, this study aimed to evaluate the in vitro antiplasmodial activity of extracts and fractions of A. indica cell culture on chloroquine-resistan...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2021-03, Vol.91 (1), p.81-88 |
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| creator | Zuleta-Castro, Carolina Ríos, Alexandra Durango, Diego Hoyos, Rodrigo Pabón, Adriana Orozco-Sánchez, Fernando |
| description | Azadirachta indica
A. Juss (neem tree) is used in Nigeria and India to control malaria. Due to the difficulty to obtain secondary metabolites from this plant, this study aimed to evaluate the in vitro antiplasmodial activity of extracts and fractions of
A. indica
cell culture on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains.
A. indica
cell culture was carried on in stirred tank bioreactor. Biomass from this process was extracted with ethanol and subsequently fractionated with different solvents. Ethanolic extract and fractions obtained were tested in bioassays on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains. Chloroquine was used as positive control. Finally, hexane fraction was analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to tentatively identify some compounds that might be involved in antiplasmodial activity. The extract showed promising activity with a half-maximal (50%) inhibitory concentration (IC
50
) of 6.14 μg ml
−1
, which decreased with the time (IC
50
: 33.64 µg ml
−1
for at > 18 months). Some extract fractions showed impressive activity against
P. falciparum
with IC
50
values of 13.66 and 9.80 µg ml
−1
for the hexane and dichloromethane fractions at > 18 months, which was higher than that of the crude extract during the same time frame. Also, 16 compounds were partially identified in hexane fraction. This is the first study reporting in vitro antiplasmodial activity of extracts of
A. indica
obtained by biotechnology way. The in vitro antiplasmodial activity of the
A. indica
cell culture ethanolic extract was demonstrated, and this extract could be a potential future alternative for treating and controlling malaria. |
| doi_str_mv | 10.1007/s40011-020-01204-z |
| format | Article |
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A. Juss (neem tree) is used in Nigeria and India to control malaria. Due to the difficulty to obtain secondary metabolites from this plant, this study aimed to evaluate the in vitro antiplasmodial activity of extracts and fractions of
A. indica
cell culture on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains.
A. indica
cell culture was carried on in stirred tank bioreactor. Biomass from this process was extracted with ethanol and subsequently fractionated with different solvents. Ethanolic extract and fractions obtained were tested in bioassays on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains. Chloroquine was used as positive control. Finally, hexane fraction was analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to tentatively identify some compounds that might be involved in antiplasmodial activity. The extract showed promising activity with a half-maximal (50%) inhibitory concentration (IC
50
) of 6.14 μg ml
−1
, which decreased with the time (IC
50
: 33.64 µg ml
−1
for at > 18 months). Some extract fractions showed impressive activity against
P. falciparum
with IC
50
values of 13.66 and 9.80 µg ml
−1
for the hexane and dichloromethane fractions at > 18 months, which was higher than that of the crude extract during the same time frame. Also, 16 compounds were partially identified in hexane fraction. This is the first study reporting in vitro antiplasmodial activity of extracts of
A. indica
obtained by biotechnology way. The in vitro antiplasmodial activity of the
A. indica
cell culture ethanolic extract was demonstrated, and this extract could be a potential future alternative for treating and controlling malaria.</description><identifier>ISSN: 0369-8211</identifier><identifier>EISSN: 2250-1746</identifier><identifier>DOI: 10.1007/s40011-020-01204-z</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Antiprotozoal agents ; Azadirachta indica ; Behavioral Sciences ; Biomedical and Life Sciences ; Bioreactors ; Biotechnology ; Cell culture ; Chloroquine ; Dichloromethane ; Ethanol ; Life Sciences ; Liquid chromatography ; Malaria ; Mass spectroscopy ; Metabolites ; Neem ; Nucleic Acid Chemistry ; Plant Biochemistry ; Plasmodium falciparum ; Research Article ; Secondary metabolites</subject><ispartof>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences, 2021-03, Vol.91 (1), p.81-88</ispartof><rights>The National Academy of Sciences, India 2020</rights><rights>The National Academy of Sciences, India 2020.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c234z-a7724526fabe17d070c8014c991125f4e47ed6bbd89dd7e57f3b27abb22a8d1a3</citedby><cites>FETCH-LOGICAL-c234z-a7724526fabe17d070c8014c991125f4e47ed6bbd89dd7e57f3b27abb22a8d1a3</cites><orcidid>0000-0002-4833-8698 ; 0000-0002-4577-0557 ; 0000-0002-0231-1940 ; 0000-0002-5535-0708 ; 0000-0003-1706-9808 ; 0000-0003-3575-1253</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40011-020-01204-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40011-020-01204-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Zuleta-Castro, Carolina</creatorcontrib><creatorcontrib>Ríos, Alexandra</creatorcontrib><creatorcontrib>Durango, Diego</creatorcontrib><creatorcontrib>Hoyos, Rodrigo</creatorcontrib><creatorcontrib>Pabón, Adriana</creatorcontrib><creatorcontrib>Orozco-Sánchez, Fernando</creatorcontrib><title>In Vitro Antiplasmodial Activity of an Azadirachta indica Cell Culture Extract</title><title>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</title><addtitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</addtitle><description>Azadirachta indica
A. Juss (neem tree) is used in Nigeria and India to control malaria. Due to the difficulty to obtain secondary metabolites from this plant, this study aimed to evaluate the in vitro antiplasmodial activity of extracts and fractions of
A. indica
cell culture on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains.
A. indica
cell culture was carried on in stirred tank bioreactor. Biomass from this process was extracted with ethanol and subsequently fractionated with different solvents. Ethanolic extract and fractions obtained were tested in bioassays on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains. Chloroquine was used as positive control. Finally, hexane fraction was analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to tentatively identify some compounds that might be involved in antiplasmodial activity. The extract showed promising activity with a half-maximal (50%) inhibitory concentration (IC
50
) of 6.14 μg ml
−1
, which decreased with the time (IC
50
: 33.64 µg ml
−1
for at > 18 months). Some extract fractions showed impressive activity against
P. falciparum
with IC
50
values of 13.66 and 9.80 µg ml
−1
for the hexane and dichloromethane fractions at > 18 months, which was higher than that of the crude extract during the same time frame. Also, 16 compounds were partially identified in hexane fraction. This is the first study reporting in vitro antiplasmodial activity of extracts of
A. indica
obtained by biotechnology way. The in vitro antiplasmodial activity of the
A. indica
cell culture ethanolic extract was demonstrated, and this extract could be a potential future alternative for treating and controlling malaria.</description><subject>Antiprotozoal agents</subject><subject>Azadirachta indica</subject><subject>Behavioral Sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Chloroquine</subject><subject>Dichloromethane</subject><subject>Ethanol</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Malaria</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Neem</subject><subject>Nucleic Acid Chemistry</subject><subject>Plant Biochemistry</subject><subject>Plasmodium falciparum</subject><subject>Research Article</subject><subject>Secondary metabolites</subject><issn>0369-8211</issn><issn>2250-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kDFPwzAQhS0EElXpH2CyxGw4O06cjFFUoFIFC7BaTuyAqzQptoNofj2GILFxyw333runD6FLCtcUQNx4DkApAQYEKANOphO0YCwFQgXPTtECkqwgOaP0HK2830GcLAXK8wV62PT4xQY34LIP9tApvx-0VR0um2A_bDjiocWqx-WktHWqeQsK217bRuHKdB2uxi6MzuD1Z4jXcIHOWtV5s_rdS_R8u36q7sn28W5TlVvSsIRPRAnBeMqyVtWGCg0Cmjz2aYqCUpa23HBhdFbXOi-0FiYVbVIzoeqaMZVrqpIluppzD254H40PcjeMro8vJeOFSEWaAUQVm1WNG7x3ppUHZ_fKHSUF-Y1OzuhkRCd_0MkpmpLZ5KO4fzXuL_of1xe0QHET</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Zuleta-Castro, Carolina</creator><creator>Ríos, Alexandra</creator><creator>Durango, Diego</creator><creator>Hoyos, Rodrigo</creator><creator>Pabón, Adriana</creator><creator>Orozco-Sánchez, Fernando</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-4833-8698</orcidid><orcidid>https://orcid.org/0000-0002-4577-0557</orcidid><orcidid>https://orcid.org/0000-0002-0231-1940</orcidid><orcidid>https://orcid.org/0000-0002-5535-0708</orcidid><orcidid>https://orcid.org/0000-0003-1706-9808</orcidid><orcidid>https://orcid.org/0000-0003-3575-1253</orcidid></search><sort><creationdate>20210301</creationdate><title>In Vitro Antiplasmodial Activity of an Azadirachta indica Cell Culture Extract</title><author>Zuleta-Castro, Carolina ; Ríos, Alexandra ; Durango, Diego ; Hoyos, Rodrigo ; Pabón, Adriana ; Orozco-Sánchez, Fernando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c234z-a7724526fabe17d070c8014c991125f4e47ed6bbd89dd7e57f3b27abb22a8d1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antiprotozoal agents</topic><topic>Azadirachta indica</topic><topic>Behavioral Sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Chloroquine</topic><topic>Dichloromethane</topic><topic>Ethanol</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Malaria</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Neem</topic><topic>Nucleic Acid Chemistry</topic><topic>Plant Biochemistry</topic><topic>Plasmodium falciparum</topic><topic>Research Article</topic><topic>Secondary metabolites</topic><toplevel>online_resources</toplevel><creatorcontrib>Zuleta-Castro, Carolina</creatorcontrib><creatorcontrib>Ríos, Alexandra</creatorcontrib><creatorcontrib>Durango, Diego</creatorcontrib><creatorcontrib>Hoyos, Rodrigo</creatorcontrib><creatorcontrib>Pabón, Adriana</creatorcontrib><creatorcontrib>Orozco-Sánchez, Fernando</creatorcontrib><collection>CrossRef</collection><jtitle>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zuleta-Castro, Carolina</au><au>Ríos, Alexandra</au><au>Durango, Diego</au><au>Hoyos, Rodrigo</au><au>Pabón, Adriana</au><au>Orozco-Sánchez, Fernando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Antiplasmodial Activity of an Azadirachta indica Cell Culture Extract</atitle><jtitle>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</jtitle><stitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</stitle><date>2021-03-01</date><risdate>2021</risdate><volume>91</volume><issue>1</issue><spage>81</spage><epage>88</epage><pages>81-88</pages><issn>0369-8211</issn><eissn>2250-1746</eissn><abstract>Azadirachta indica
A. Juss (neem tree) is used in Nigeria and India to control malaria. Due to the difficulty to obtain secondary metabolites from this plant, this study aimed to evaluate the in vitro antiplasmodial activity of extracts and fractions of
A. indica
cell culture on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains.
A. indica
cell culture was carried on in stirred tank bioreactor. Biomass from this process was extracted with ethanol and subsequently fractionated with different solvents. Ethanolic extract and fractions obtained were tested in bioassays on chloroquine-resistant
Plasmodium falciparum
(FCR3) strains. Chloroquine was used as positive control. Finally, hexane fraction was analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, to tentatively identify some compounds that might be involved in antiplasmodial activity. The extract showed promising activity with a half-maximal (50%) inhibitory concentration (IC
50
) of 6.14 μg ml
−1
, which decreased with the time (IC
50
: 33.64 µg ml
−1
for at > 18 months). Some extract fractions showed impressive activity against
P. falciparum
with IC
50
values of 13.66 and 9.80 µg ml
−1
for the hexane and dichloromethane fractions at > 18 months, which was higher than that of the crude extract during the same time frame. Also, 16 compounds were partially identified in hexane fraction. This is the first study reporting in vitro antiplasmodial activity of extracts of
A. indica
obtained by biotechnology way. The in vitro antiplasmodial activity of the
A. indica
cell culture ethanolic extract was demonstrated, and this extract could be a potential future alternative for treating and controlling malaria.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s40011-020-01204-z</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4833-8698</orcidid><orcidid>https://orcid.org/0000-0002-4577-0557</orcidid><orcidid>https://orcid.org/0000-0002-0231-1940</orcidid><orcidid>https://orcid.org/0000-0002-5535-0708</orcidid><orcidid>https://orcid.org/0000-0003-1706-9808</orcidid><orcidid>https://orcid.org/0000-0003-3575-1253</orcidid></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences, 2021-03, Vol.91 (1), p.81-88 |
| issn | 0369-8211 2250-1746 |
| language | eng |
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| subjects | Antiprotozoal agents Azadirachta indica Behavioral Sciences Biomedical and Life Sciences Bioreactors Biotechnology Cell culture Chloroquine Dichloromethane Ethanol Life Sciences Liquid chromatography Malaria Mass spectroscopy Metabolites Neem Nucleic Acid Chemistry Plant Biochemistry Plasmodium falciparum Research Article Secondary metabolites |
| title | In Vitro Antiplasmodial Activity of an Azadirachta indica Cell Culture Extract |
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