Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

Using the children's toy, Shrinky-Dink©, we present an aptamer-based electrochemical (E-AB) assay that recognizes the spike protein of SARS-CoV-2 in saliva for viral infection detection. The low-cost electrodes are implementable at population scale and demonstrate detection down to 1 ag mL −1 o...

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Veröffentlicht in:	Analytical methods 2021-02, Vol.13 (7), p.874-883
Hauptverfasser:	Zakashansky, Julia A, Imamura, Amanda H, Salgado, Darwin F, Romero Mercieca, Heather C, Aguas, Raphael F. L, Lao, Angelou M, Pariser, Joseph, Arroyo-Currás, Netzahualcóyotl, Khine, Michelle
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamers Aptamers, Nucleotide - chemistry Biosensing Techniques - methods Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Electrochemistry Electrodes Gold - chemistry Humans Limit of Detection Polystyrenes - chemistry Protein Domains Proteins Saliva Saliva - chemistry SARS-CoV-2 - chemistry Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - analysis Spike Glycoprotein, Coronavirus - chemistry Spike protein Viral diseases
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Beschreibung
Zusammenfassung:	Using the children's toy, Shrinky-Dink©, we present an aptamer-based electrochemical (E-AB) assay that recognizes the spike protein of SARS-CoV-2 in saliva for viral infection detection. The low-cost electrodes are implementable at population scale and demonstrate detection down to 1 ag mL −1 of the S1 subunit of the spike protein. We introduce a simple, scalable, and portable electrochemical aptamer-based system for detection of the SARS-CoV-2 spike protein in saliva with attomolar sensitivity.
ISSN:	1759-9660 1759-9679
DOI:	10.1039/d1ay00041a