CRISPR‐Cas9 mediated engineering of Bacillus licheniformis for industrial production of (2R,3S)‐butanediol

Bacillus lichenformis is an industrially promising generally recognized as safe (GRAS) strain that can be used for the production of a valuable chemical, 2,3‐butanediol (BDO). Conventional gene deletion vectors and/or methods are time‐consuming and have poor efficiency. Therefore, clustered regularl...

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Veröffentlicht in:	Biotechnology progress 2021-01, Vol.37 (1), p.e3072-n/a
Hauptverfasser:	Song, Chan Woo, Rathnasingh, Chelladurai, Park, Jong Myoung, Kwon, Mina, Song, Hyohak
Format:	Artikel
Sprache:	eng
Schlagworte:	2,3‐butanediol Bacillus licheniformis Bacillus licheniformis - genetics Bacillus licheniformis - growth & development Bacillus licheniformis - metabolism Bacterial Proteins - antagonists & inhibitors Bacterial Proteins - genetics Bacterial Proteins - metabolism Butanediol Butylene Glycols - metabolism CRISPR CRISPR-Cas Systems CRISPR‐Cas9 Deletion mutant Fermentation Gene deletion GTP-Binding Proteins - antagonists & inhibitors GTP-Binding Proteins - genetics GTP-Binding Proteins - metabolism Homologous recombination Homology Industrial production Isocitrate Dehydrogenase - antagonists & inhibitors Isocitrate Dehydrogenase - genetics Isocitrate Dehydrogenase - metabolism LDH gene Metabolic Engineering Mutants Mutation selectivity Vectors
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