Label-Free Detection of C–T Mutations by Surface-Enhanced Raman Spectroscopy Using Thiosulfate-Modified Nanoparticles
Recent developments in molecular spectroscopy have widened the scope of surface-enhanced Raman spectroscopy (SERS) for detection of nucleic acids. In order to solve the interference of impurity signals in SERS analysis that hamper the reliable detection of DNA, Ag nanoparticles modified with thiosul...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2021-02, Vol.93 (4), p.1951-1956 |
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| container_end_page | 1956 |
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| container_issue | 4 |
| container_start_page | 1951 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 93 |
| creator | Zeng, Jiayu Dong, Meiyu Zhu, Bixue Gao, Xin Chen, Dongmei Li, Yang |
| description | Recent developments in molecular spectroscopy have widened the scope of surface-enhanced Raman spectroscopy (SERS) for detection of nucleic acids. In order to solve the interference of impurity signals in SERS analysis that hamper the reliable detection of DNA, Ag nanoparticles modified with thiosulfate ions were used to obtain SERS signals of DNA molecules in aqueous solutions, which showed good reproducibility. By using thiosulfate ions and calcium ions as aggregating agents, this method not only eliminated the influence of citrate on DNA signals completely but also obtained the signals for all bases indiscriminately, including the T base that was considered to have low Raman activity. Subsequently, the base stacking rule was used to identify mutations arising from C/T transition. It further identified the mutation sites of single-base C/T transition using this platform for the first time. This method has wide application prospects in DNA analysis, DNA sequencing, and genetic testing. |
| doi_str_mv | 10.1021/acs.analchem.0c04052 |
| format | Article |
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In order to solve the interference of impurity signals in SERS analysis that hamper the reliable detection of DNA, Ag nanoparticles modified with thiosulfate ions were used to obtain SERS signals of DNA molecules in aqueous solutions, which showed good reproducibility. By using thiosulfate ions and calcium ions as aggregating agents, this method not only eliminated the influence of citrate on DNA signals completely but also obtained the signals for all bases indiscriminately, including the T base that was considered to have low Raman activity. Subsequently, the base stacking rule was used to identify mutations arising from C/T transition. It further identified the mutation sites of single-base C/T transition using this platform for the first time. This method has wide application prospects in DNA analysis, DNA sequencing, and genetic testing.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.0c04052</identifier><identifier>PMID: 33464044</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aqueous solutions ; Base stacking ; Calcium ; Calcium ions ; Chemistry ; Citric acid ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; DNA sequencing ; Genetic analysis ; Genetic screening ; Ions ; Metal Nanoparticles - chemistry ; Molecular spectroscopy ; Mutation ; Nanoparticles ; Nucleic acids ; Raman spectroscopy ; Silver ; Silver - chemistry ; Spectroscopy ; Spectrum analysis ; Spectrum Analysis, Raman - methods ; Thiosulfate ; Thiosulfates ; Thiosulfates - chemistry</subject><ispartof>Analytical chemistry (Washington), 2021-02, Vol.93 (4), p.1951-1956</ispartof><rights>2021 American Chemical Society</rights><rights>Copyright American Chemical Society Feb 2, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-9c94f62ce4d1f39a042bc4daf3a884335a9880035d38c32940cf02839906a9f83</citedby><cites>FETCH-LOGICAL-a376t-9c94f62ce4d1f39a042bc4daf3a884335a9880035d38c32940cf02839906a9f83</cites><orcidid>0000-0002-0677-3245</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c04052$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c04052$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33464044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Jiayu</creatorcontrib><creatorcontrib>Dong, Meiyu</creatorcontrib><creatorcontrib>Zhu, Bixue</creatorcontrib><creatorcontrib>Gao, Xin</creatorcontrib><creatorcontrib>Chen, Dongmei</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><title>Label-Free Detection of C–T Mutations by Surface-Enhanced Raman Spectroscopy Using Thiosulfate-Modified Nanoparticles</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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| subjects | Aqueous solutions Base stacking Calcium Calcium ions Chemistry Citric acid Deoxyribonucleic acid DNA DNA - chemistry DNA sequencing Genetic analysis Genetic screening Ions Metal Nanoparticles - chemistry Molecular spectroscopy Mutation Nanoparticles Nucleic acids Raman spectroscopy Silver Silver - chemistry Spectroscopy Spectrum analysis Spectrum Analysis, Raman - methods Thiosulfate Thiosulfates Thiosulfates - chemistry |
| title | Label-Free Detection of C–T Mutations by Surface-Enhanced Raman Spectroscopy Using Thiosulfate-Modified Nanoparticles |
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