Development of ROS‐responsive amino acid‐based poly(ester amide) nanoparticle for anticancer drug delivery
Reactive oxygen species (ROS) play an important role in cellular metabolism and many oxidative stress related diseases. Oxidative stress results from toxic effects of ROS and plays a critical role in the pathogenesis of a variety of diseases like cancers and many important biological processes. It i...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2021-04, Vol.109 (4), p.524-537 |
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| description | Reactive oxygen species (ROS) play an important role in cellular metabolism and many oxidative stress related diseases. Oxidative stress results from toxic effects of ROS and plays a critical role in the pathogenesis of a variety of diseases like cancers and many important biological processes. It is known that the unique feature of high intracellular ROS level in cancer cells can be considered as target and utilized as a useful cancer‐related stimulus to mediate intracellular drug delivery. Therefore, biomaterials responsive to excess level of ROS are of great importance in biomedical applications. In this study, a novel ROS‐responsive polymer based on L‐methionine poly(ester amide) (Met‐PEA‐PEG) was designed, synthesized, characterized and self‐assembled into nano‐micellar‐type nanoparticles (NP). The Met‐PEA‐PEG NP exhibited responsiveness to an oxidative environment. The size and morphology of the nanoparticle changed rapidly in the presence of H2O2. The Nile Red dye was loaded into the Met‐PEA‐PEG NP to demonstrate a H2O2 concentration induced time‐dependent release behavior. The Met‐PEA‐PEG NP was sensitive to high intracellular ROS level of PC3 prostate cancer cells. Furthermore, the Met‐PEA‐PEG NP was investigated as a carrier of a Chinese medicine‐based anticancer component, gambogic acid (GA). Compared to free GA, the GA‐loaded nanocomplex (GA‐NP) showed enhanced cytotoxicity toward PC3 and HeLa cells. The GA‐NP also induced a higher level of apoptosis and mitochondrial depolarization in PC3 cells than free GA. The Met‐PEA‐PEG NP improved the therapeutic effect of GA and may serve as a potential carrier for anticancer drug delivery. |
| doi_str_mv | 10.1002/jbm.a.37035 |
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Oxidative stress results from toxic effects of ROS and plays a critical role in the pathogenesis of a variety of diseases like cancers and many important biological processes. It is known that the unique feature of high intracellular ROS level in cancer cells can be considered as target and utilized as a useful cancer‐related stimulus to mediate intracellular drug delivery. Therefore, biomaterials responsive to excess level of ROS are of great importance in biomedical applications. In this study, a novel ROS‐responsive polymer based on L‐methionine poly(ester amide) (Met‐PEA‐PEG) was designed, synthesized, characterized and self‐assembled into nano‐micellar‐type nanoparticles (NP). The Met‐PEA‐PEG NP exhibited responsiveness to an oxidative environment. The size and morphology of the nanoparticle changed rapidly in the presence of H2O2. The Nile Red dye was loaded into the Met‐PEA‐PEG NP to demonstrate a H2O2 concentration induced time‐dependent release behavior. The Met‐PEA‐PEG NP was sensitive to high intracellular ROS level of PC3 prostate cancer cells. Furthermore, the Met‐PEA‐PEG NP was investigated as a carrier of a Chinese medicine‐based anticancer component, gambogic acid (GA). Compared to free GA, the GA‐loaded nanocomplex (GA‐NP) showed enhanced cytotoxicity toward PC3 and HeLa cells. The GA‐NP also induced a higher level of apoptosis and mitochondrial depolarization in PC3 cells than free GA. The Met‐PEA‐PEG NP improved the therapeutic effect of GA and may serve as a potential carrier for anticancer drug delivery.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.37035</identifier><identifier>PMID: 32529749</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Amino acids ; Antineoplastic Agents - administration & dosage ; Antineoplastic Agents - pharmacology ; Apoptosis ; Biocompatibility ; Biological activity ; Biomaterials ; Biomedical materials ; Cancer ; cancer therapy ; Cytotoxicity ; Delayed-Action Preparations - chemistry ; Delayed-Action Preparations - metabolism ; Depolarization ; Drug delivery ; Drug Delivery Systems ; gambogic acid ; HeLa Cells ; Herbal medicine ; Humans ; Hydrogen peroxide ; Intracellular ; L‐methionine‐based poly(ester amide) ; Methionine ; Methionine - analogs & derivatives ; Methionine - metabolism ; Mitochondria ; Morphology ; nanoparticle ; Nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - metabolism ; Neoplasms - drug therapy ; Neoplasms - metabolism ; Oxidative metabolism ; Oxidative stress ; Pathogenesis ; PC-3 Cells ; Polyesteramides ; Polyesters - chemistry ; Polyesters - metabolism ; Polyethylene glycol ; Polymers ; Prostate cancer ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; ROS‐responsive ; Toxicity ; Xanthones - administration & dosage ; Xanthones - pharmacology</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Reactive oxygen species (ROS) play an important role in cellular metabolism and many oxidative stress related diseases. Oxidative stress results from toxic effects of ROS and plays a critical role in the pathogenesis of a variety of diseases like cancers and many important biological processes. It is known that the unique feature of high intracellular ROS level in cancer cells can be considered as target and utilized as a useful cancer‐related stimulus to mediate intracellular drug delivery. Therefore, biomaterials responsive to excess level of ROS are of great importance in biomedical applications. In this study, a novel ROS‐responsive polymer based on L‐methionine poly(ester amide) (Met‐PEA‐PEG) was designed, synthesized, characterized and self‐assembled into nano‐micellar‐type nanoparticles (NP). The Met‐PEA‐PEG NP exhibited responsiveness to an oxidative environment. The size and morphology of the nanoparticle changed rapidly in the presence of H2O2. The Nile Red dye was loaded into the Met‐PEA‐PEG NP to demonstrate a H2O2 concentration induced time‐dependent release behavior. The Met‐PEA‐PEG NP was sensitive to high intracellular ROS level of PC3 prostate cancer cells. Furthermore, the Met‐PEA‐PEG NP was investigated as a carrier of a Chinese medicine‐based anticancer component, gambogic acid (GA). Compared to free GA, the GA‐loaded nanocomplex (GA‐NP) showed enhanced cytotoxicity toward PC3 and HeLa cells. The GA‐NP also induced a higher level of apoptosis and mitochondrial depolarization in PC3 cells than free GA. The Met‐PEA‐PEG NP improved the therapeutic effect of GA and may serve as a potential carrier for anticancer drug delivery.</description><subject>Amino acids</subject><subject>Antineoplastic Agents - administration & dosage</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Cancer</subject><subject>cancer therapy</subject><subject>Cytotoxicity</subject><subject>Delayed-Action Preparations - chemistry</subject><subject>Delayed-Action Preparations - metabolism</subject><subject>Depolarization</subject><subject>Drug delivery</subject><subject>Drug Delivery Systems</subject><subject>gambogic acid</subject><subject>HeLa Cells</subject><subject>Herbal medicine</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Intracellular</subject><subject>L‐methionine‐based poly(ester amide)</subject><subject>Methionine</subject><subject>Methionine - analogs & derivatives</subject><subject>Methionine - metabolism</subject><subject>Mitochondria</subject><subject>Morphology</subject><subject>nanoparticle</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - metabolism</subject><subject>Neoplasms - drug therapy</subject><subject>Neoplasms - metabolism</subject><subject>Oxidative metabolism</subject><subject>Oxidative stress</subject><subject>Pathogenesis</subject><subject>PC-3 Cells</subject><subject>Polyesteramides</subject><subject>Polyesters - chemistry</subject><subject>Polyesters - metabolism</subject><subject>Polyethylene glycol</subject><subject>Polymers</subject><subject>Prostate cancer</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>ROS‐responsive</subject><subject>Toxicity</subject><subject>Xanthones - administration & dosage</subject><subject>Xanthones - pharmacology</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQhi0EYj9xR5G4gFCK1yQ-lh1UhMRytpx4glIldrDbot54BJ6RJ8GlwJHTjGY-fTP6EdojeEAwpifjshvoAcsxEytokwhBUy4zsbrouUwZldkG2gphHOEMC7qONhgVVOZcbiJ7DjNoXd-BnSSuTh7uHz_fPzyE3tnQzCDRXWNdoqvGxHmpA5ikd-38EMIE_GJr4Cix2rpe-0lTtZDULs5t7LWtImL89CUx0EaZn--gtVq3AXZ_6jZ6vrx4OrtOR_dXN2fDUVrRXIg0k5hVXBNeUoEZloCxJEKWRWGqAhtBOBjMccY1zbWQuRQ0g6IGJgkjghC2jQ6W3t6712n8VY3d1Nt4UlEuoy1qi0gdL6nKuxA81Kr3Taf9XBGsFtmqmK3S6jvbSO__OKdlB-aP_Q0zAnQJvDUtzP9zqdvTu-HS-gVY5IW-</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Xu, Qinghua</creator><creator>Chu, Chih‐Chang</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-0530-3337</orcidid></search><sort><creationdate>202104</creationdate><title>Development of ROS‐responsive amino acid‐based poly(ester amide) nanoparticle for anticancer drug delivery</title><author>Xu, Qinghua ; Chu, Chih‐Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2755-6903c4a14b250309e009159b88dc80d514ed04064a27a5979526e8fe391315113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amino acids</topic><topic>Antineoplastic Agents - administration & dosage</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Biocompatibility</topic><topic>Biological activity</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Cancer</topic><topic>cancer therapy</topic><topic>Cytotoxicity</topic><topic>Delayed-Action Preparations - chemistry</topic><topic>Delayed-Action Preparations - metabolism</topic><topic>Depolarization</topic><topic>Drug delivery</topic><topic>Drug Delivery Systems</topic><topic>gambogic acid</topic><topic>HeLa Cells</topic><topic>Herbal medicine</topic><topic>Humans</topic><topic>Hydrogen peroxide</topic><topic>Intracellular</topic><topic>L‐methionine‐based poly(ester amide)</topic><topic>Methionine</topic><topic>Methionine - analogs & derivatives</topic><topic>Methionine - metabolism</topic><topic>Mitochondria</topic><topic>Morphology</topic><topic>nanoparticle</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - metabolism</topic><topic>Neoplasms - drug therapy</topic><topic>Neoplasms - metabolism</topic><topic>Oxidative metabolism</topic><topic>Oxidative stress</topic><topic>Pathogenesis</topic><topic>PC-3 Cells</topic><topic>Polyesteramides</topic><topic>Polyesters - chemistry</topic><topic>Polyesters - metabolism</topic><topic>Polyethylene glycol</topic><topic>Polymers</topic><topic>Prostate cancer</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>ROS‐responsive</topic><topic>Toxicity</topic><topic>Xanthones - administration & dosage</topic><topic>Xanthones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Qinghua</creatorcontrib><creatorcontrib>Chu, Chih‐Chang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Qinghua</au><au>Chu, Chih‐Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of ROS‐responsive amino acid‐based poly(ester amide) nanoparticle for anticancer drug delivery</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J Biomed Mater Res A</addtitle><date>2021-04</date><risdate>2021</risdate><volume>109</volume><issue>4</issue><spage>524</spage><epage>537</epage><pages>524-537</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Reactive oxygen species (ROS) play an important role in cellular metabolism and many oxidative stress related diseases. Oxidative stress results from toxic effects of ROS and plays a critical role in the pathogenesis of a variety of diseases like cancers and many important biological processes. It is known that the unique feature of high intracellular ROS level in cancer cells can be considered as target and utilized as a useful cancer‐related stimulus to mediate intracellular drug delivery. Therefore, biomaterials responsive to excess level of ROS are of great importance in biomedical applications. In this study, a novel ROS‐responsive polymer based on L‐methionine poly(ester amide) (Met‐PEA‐PEG) was designed, synthesized, characterized and self‐assembled into nano‐micellar‐type nanoparticles (NP). The Met‐PEA‐PEG NP exhibited responsiveness to an oxidative environment. The size and morphology of the nanoparticle changed rapidly in the presence of H2O2. The Nile Red dye was loaded into the Met‐PEA‐PEG NP to demonstrate a H2O2 concentration induced time‐dependent release behavior. The Met‐PEA‐PEG NP was sensitive to high intracellular ROS level of PC3 prostate cancer cells. Furthermore, the Met‐PEA‐PEG NP was investigated as a carrier of a Chinese medicine‐based anticancer component, gambogic acid (GA). Compared to free GA, the GA‐loaded nanocomplex (GA‐NP) showed enhanced cytotoxicity toward PC3 and HeLa cells. The GA‐NP also induced a higher level of apoptosis and mitochondrial depolarization in PC3 cells than free GA. The Met‐PEA‐PEG NP improved the therapeutic effect of GA and may serve as a potential carrier for anticancer drug delivery.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>32529749</pmid><doi>10.1002/jbm.a.37035</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-0530-3337</orcidid></addata></record> |
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| subjects | Amino acids Antineoplastic Agents - administration & dosage Antineoplastic Agents - pharmacology Apoptosis Biocompatibility Biological activity Biomaterials Biomedical materials Cancer cancer therapy Cytotoxicity Delayed-Action Preparations - chemistry Delayed-Action Preparations - metabolism Depolarization Drug delivery Drug Delivery Systems gambogic acid HeLa Cells Herbal medicine Humans Hydrogen peroxide Intracellular L‐methionine‐based poly(ester amide) Methionine Methionine - analogs & derivatives Methionine - metabolism Mitochondria Morphology nanoparticle Nanoparticles Nanoparticles - chemistry Nanoparticles - metabolism Neoplasms - drug therapy Neoplasms - metabolism Oxidative metabolism Oxidative stress Pathogenesis PC-3 Cells Polyesteramides Polyesters - chemistry Polyesters - metabolism Polyethylene glycol Polymers Prostate cancer Reactive oxygen species Reactive Oxygen Species - metabolism ROS‐responsive Toxicity Xanthones - administration & dosage Xanthones - pharmacology |
| title | Development of ROS‐responsive amino acid‐based poly(ester amide) nanoparticle for anticancer drug delivery |
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