Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport
A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biom...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2021-02, Vol.60 (9), p.4907-4914 |
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| creator | Ma, Cheng Wu, Shaojun Zhou, Yang Wei, Hui‐Fang Zhang, Jianrong Chen, Zixuan Zhu, Jun‐Jie Lin, Yuehe Zhu, Wenlei |
| description | A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR‐1.
Amine‐rich biomolecules as consumed coreactants drive electrochemiluminescence with Ru(bpy)32+, enabling bio‐coreactant‐enhanced single‐cell electrochemiluminescence microscopy. This allows the imaging of intracellular hierarchical structures without the use of multiple labels. Dynamic signals disclose the universal edge effect of cellular electroporation and enable the visualization of heterogeneous molecular transport. |
| doi_str_mv | 10.1002/anie.202012171 |
| format | Article |
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Amine‐rich biomolecules as consumed coreactants drive electrochemiluminescence with Ru(bpy)32+, enabling bio‐coreactant‐enhanced single‐cell electrochemiluminescence microscopy. This allows the imaging of intracellular hierarchical structures without the use of multiple labels. Dynamic signals disclose the universal edge effect of cellular electroporation and enable the visualization of heterogeneous molecular transport.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.202012171</identifier><identifier>PMID: 33188721</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Actin ; Animals ; Autophagy ; bioelectrochemistry ; Biomolecules ; Cell membranes ; Cytoskeleton ; Damage detection ; DNA damage ; DNA Damage - drug effects ; Edge effect ; Electrochemical Techniques ; Electrochemiluminescence ; Electrochemistry ; Electrodes ; Electroporation ; HeLa Cells ; Humans ; Image enhancement ; Image processing ; Intracellular ; Liver - microbiology ; Liver - pathology ; Luminescent Measurements ; Mice ; Microscopy ; Microscopy, Atomic Force ; Microscopy, Fluorescence - methods ; Organometallic Compounds - chemistry ; Organometallic Compounds - pharmacology ; Phagocytosis ; Reactive Oxygen Species - metabolism ; Shewanella - isolation & purification ; Single-Cell Analysis ; single-cell studies</subject><ispartof>Angewandte Chemie International Edition, 2021-02, Vol.60 (9), p.4907-4914</ispartof><rights>2020 Wiley‐VCH GmbH</rights><rights>2020 Wiley-VCH GmbH.</rights><rights>2021 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4761-962ea295f8664c94766def9f28cf36fce7ed2c099484ff31d8d338a389ba2e53</citedby><cites>FETCH-LOGICAL-c4761-962ea295f8664c94766def9f28cf36fce7ed2c099484ff31d8d338a389ba2e53</cites><orcidid>0000-0003-3791-7587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.202012171$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.202012171$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33188721$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Cheng</creatorcontrib><creatorcontrib>Wu, Shaojun</creatorcontrib><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Wei, Hui‐Fang</creatorcontrib><creatorcontrib>Zhang, Jianrong</creatorcontrib><creatorcontrib>Chen, Zixuan</creatorcontrib><creatorcontrib>Zhu, Jun‐Jie</creatorcontrib><creatorcontrib>Lin, Yuehe</creatorcontrib><creatorcontrib>Zhu, Wenlei</creatorcontrib><title>Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport</title><title>Angewandte Chemie International Edition</title><addtitle>Angew Chem Int Ed Engl</addtitle><description>A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR‐1.
Amine‐rich biomolecules as consumed coreactants drive electrochemiluminescence with Ru(bpy)32+, enabling bio‐coreactant‐enhanced single‐cell electrochemiluminescence microscopy. This allows the imaging of intracellular hierarchical structures without the use of multiple labels. Dynamic signals disclose the universal edge effect of cellular electroporation and enable the visualization of heterogeneous molecular transport.</description><subject>Actin</subject><subject>Animals</subject><subject>Autophagy</subject><subject>bioelectrochemistry</subject><subject>Biomolecules</subject><subject>Cell membranes</subject><subject>Cytoskeleton</subject><subject>Damage detection</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Edge effect</subject><subject>Electrochemical Techniques</subject><subject>Electrochemiluminescence</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Electroporation</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Image enhancement</subject><subject>Image processing</subject><subject>Intracellular</subject><subject>Liver - microbiology</subject><subject>Liver - pathology</subject><subject>Luminescent Measurements</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Organometallic Compounds - chemistry</subject><subject>Organometallic Compounds - pharmacology</subject><subject>Phagocytosis</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Shewanella - isolation & purification</subject><subject>Single-Cell Analysis</subject><subject>single-cell studies</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOwzAUhi0EoqWwMqJIzCm-5GKPpQpQqcBAd8t1jtVUiV2cRKgbj8Az8iS4aikj07l95z86P0LXBI8JxvRO2QrGFFNMKMnJCRqSlJKY5Tk7DXnCWJzzlAzQRduuA885zs7RgDHCeU7JEK3uK_f9-TV1HpTulO1CUdiVshrKqKhBd97pFTRV3TeVhVZDmETPlfau1W6zjZyJZrbzSkNd97Xy0Vvne931HiJly2jhlW03zneX6MyouoWrQxyhxUOxmD7F89fH2XQyj3WSZyQWGQVFRWp4liVahF5WghGGcm1YZjTkUFKNhUh4YgwjJS8Z44pxsVQUUjZCt3vZjXfvPbSdXLve23BR0oSLlHBCcaDGe2r3RuvByI2vGuW3kmC581XufJVHX8PCzUG2XzZQHvFfIwMg9sBHVcP2Hzk5eZkVf-I_F32IOQ</recordid><startdate>20210223</startdate><enddate>20210223</enddate><creator>Ma, Cheng</creator><creator>Wu, Shaojun</creator><creator>Zhou, Yang</creator><creator>Wei, Hui‐Fang</creator><creator>Zhang, Jianrong</creator><creator>Chen, Zixuan</creator><creator>Zhu, Jun‐Jie</creator><creator>Lin, Yuehe</creator><creator>Zhu, Wenlei</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0003-3791-7587</orcidid></search><sort><creationdate>20210223</creationdate><title>Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport</title><author>Ma, Cheng ; Wu, Shaojun ; Zhou, Yang ; Wei, Hui‐Fang ; Zhang, Jianrong ; Chen, Zixuan ; Zhu, Jun‐Jie ; Lin, Yuehe ; Zhu, Wenlei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4761-962ea295f8664c94766def9f28cf36fce7ed2c099484ff31d8d338a389ba2e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Actin</topic><topic>Animals</topic><topic>Autophagy</topic><topic>bioelectrochemistry</topic><topic>Biomolecules</topic><topic>Cell membranes</topic><topic>Cytoskeleton</topic><topic>Damage detection</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>Edge effect</topic><topic>Electrochemical Techniques</topic><topic>Electrochemiluminescence</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Electroporation</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Image enhancement</topic><topic>Image processing</topic><topic>Intracellular</topic><topic>Liver - microbiology</topic><topic>Liver - pathology</topic><topic>Luminescent Measurements</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Organometallic Compounds - chemistry</topic><topic>Organometallic Compounds - pharmacology</topic><topic>Phagocytosis</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Shewanella - isolation & purification</topic><topic>Single-Cell Analysis</topic><topic>single-cell studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Cheng</creatorcontrib><creatorcontrib>Wu, Shaojun</creatorcontrib><creatorcontrib>Zhou, Yang</creatorcontrib><creatorcontrib>Wei, Hui‐Fang</creatorcontrib><creatorcontrib>Zhang, Jianrong</creatorcontrib><creatorcontrib>Chen, Zixuan</creatorcontrib><creatorcontrib>Zhu, Jun‐Jie</creatorcontrib><creatorcontrib>Lin, Yuehe</creatorcontrib><creatorcontrib>Zhu, Wenlei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Cheng</au><au>Wu, Shaojun</au><au>Zhou, Yang</au><au>Wei, Hui‐Fang</au><au>Zhang, Jianrong</au><au>Chen, Zixuan</au><au>Zhu, Jun‐Jie</au><au>Lin, Yuehe</au><au>Zhu, Wenlei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2021-02-23</date><risdate>2021</risdate><volume>60</volume><issue>9</issue><spage>4907</spage><epage>4914</epage><pages>4907-4914</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR‐1.
Amine‐rich biomolecules as consumed coreactants drive electrochemiluminescence with Ru(bpy)32+, enabling bio‐coreactant‐enhanced single‐cell electrochemiluminescence microscopy. This allows the imaging of intracellular hierarchical structures without the use of multiple labels. Dynamic signals disclose the universal edge effect of cellular electroporation and enable the visualization of heterogeneous molecular transport.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33188721</pmid><doi>10.1002/anie.202012171</doi><tpages>8</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0003-3791-7587</orcidid></addata></record> |
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| subjects | Actin Animals Autophagy bioelectrochemistry Biomolecules Cell membranes Cytoskeleton Damage detection DNA damage DNA Damage - drug effects Edge effect Electrochemical Techniques Electrochemiluminescence Electrochemistry Electrodes Electroporation HeLa Cells Humans Image enhancement Image processing Intracellular Liver - microbiology Liver - pathology Luminescent Measurements Mice Microscopy Microscopy, Atomic Force Microscopy, Fluorescence - methods Organometallic Compounds - chemistry Organometallic Compounds - pharmacology Phagocytosis Reactive Oxygen Species - metabolism Shewanella - isolation & purification Single-Cell Analysis single-cell studies |
| title | Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport |
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