Single step separation and concentration of biomarker proteins using agarose based miniaturized isoelectric gates for point of care diagnostics

•Isoelectric trapping using agarose pH gates for rapid runtimes at a low voltage.•Targeted separation using pH gates tuned to the isoelectric point of the biomarker.•Separation of low concentration protein in high protein load without precipitation. Bedside diagnostics using protein biomarkers requi...

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Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2021-03, Vol.330, p.129265, Article 129265
Hauptverfasser:	Damodara, Sreekant, Dwivedi, Dhruva J., Liaw, Patricia C., Fox-Robichaud, Alison E., Selvaganapathy, P. Ravi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Biomarker concentration Biomarker detection Biomarkers Complexity Electrophoresis Fluorescent microscopy Gates Gels Isoelectric focusing Low cost Low cost sensing Microfluidics Ovalbumin Point-of-care testing Protein C Proteins Separation Serum albumin
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