Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip‐based capillary electrophoresis–mass spectrometry
Protein glycosylation can impact the efficacy, safety, and pharmacokinetics of therapeutic proteins. Achieving uniform and consistent protein glycosylation is an important requirement for product quality control at all stages of therapeutic protein drug discovery and development. The development of...
Gespeichert in:
| Veröffentlicht in: | Electrophoresis 2021-02, Vol.42 (4), p.460-464 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 464 |
|---|---|
| container_issue | 4 |
| container_start_page | 460 |
| container_title | Electrophoresis |
| container_volume | 42 |
| creator | Deyanova, Ekaterina G. Huang, Richard Y.‐C. Madia, Priyanka A. Nandi, Pradyot Gudmundsson, Olafur Chen, Guodong |
| description | Protein glycosylation can impact the efficacy, safety, and pharmacokinetics of therapeutic proteins. Achieving uniform and consistent protein glycosylation is an important requirement for product quality control at all stages of therapeutic protein drug discovery and development. The development of a new microfluidic CE device compatible with MS offers a fast and sensitive orthogonal mode of high‐resolution separation with MS characterization. Here, we describe a fast and robust chip‐based CE‐MS method for intact glycosylation fingerprinting of a therapeutic fusion protein with complex sialylated N and O‐linked glycoforms. The method effectively separates multiple sialylated glycoforms and offers a rapid detection of changes in glycosylation profile in 6 min. |
| doi_str_mv | 10.1002/elps.202000132 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2488493814</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2488493814</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4054-bc4b2f1225f31b8e478e29ed100830d62e5bae185fb2d67d464ae5d4a5ac5e1a3</originalsourceid><addsrcrecordid>eNqFkMtKAzEUhoMoWqtblxJwPTXXTroU8QYFxct6yGTOtJHMxWQGmV13bgXf0Ccx2urWVQ7hO__h_xA6omRCCWGn4NowYYQRQihnW2hEJWMJmyq-jUaEpjwhiss9tB_Cc2TETIhdtMeZUlISOkJv97q1BS5tvQDfelt3ccJNiTVe2sXSDXjhBtOEwekOItcH29S49U0Htsb5gCtrfFO63hbWYLO07efqPdchsiYmO6f9gMGB6XzTLhsPwYbP1UelQ8Ch_fmuoPPDAdoptQtwuHnH6Ony4vH8OpnfXt2cn80TI4gUSW5EzkrKmCw5zRWIVAGbQRFdKE6KKQOZa6BKljkrpmkhpkKDLISW2kigmo_RyTo3VnjpIXTZc9P7Op7MmFBKzLiiIlKTNRW7heChzKKaKlbJKMm-vWff3rM_73HheBPb5xUUf_iv6AiINfBqHQz_xGUX87uHlKWCfwGfMJR4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2488493814</pqid></control><display><type>article</type><title>Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip‐based capillary electrophoresis–mass spectrometry</title><source>Wiley Online Library All Journals</source><creator>Deyanova, Ekaterina G. ; Huang, Richard Y.‐C. ; Madia, Priyanka A. ; Nandi, Pradyot ; Gudmundsson, Olafur ; Chen, Guodong</creator><creatorcontrib>Deyanova, Ekaterina G. ; Huang, Richard Y.‐C. ; Madia, Priyanka A. ; Nandi, Pradyot ; Gudmundsson, Olafur ; Chen, Guodong</creatorcontrib><description>Protein glycosylation can impact the efficacy, safety, and pharmacokinetics of therapeutic proteins. Achieving uniform and consistent protein glycosylation is an important requirement for product quality control at all stages of therapeutic protein drug discovery and development. The development of a new microfluidic CE device compatible with MS offers a fast and sensitive orthogonal mode of high‐resolution separation with MS characterization. Here, we describe a fast and robust chip‐based CE‐MS method for intact glycosylation fingerprinting of a therapeutic fusion protein with complex sialylated N and O‐linked glycoforms. The method effectively separates multiple sialylated glycoforms and offers a rapid detection of changes in glycosylation profile in 6 min.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.202000132</identifier><identifier>PMID: 32885501</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>CE‐MS ; Electrophoresis ; Fingerprinting ; Glycoprofiling ; Mass spectrometry ; Microfluidics ; Proteins ; Quality control ; Therapeutic proteins</subject><ispartof>Electrophoresis, 2021-02, Vol.42 (4), p.460-464</ispartof><rights>2020 Wiley‐VCH GmbH</rights><rights>2020 Wiley-VCH GmbH.</rights><rights>2021 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4054-bc4b2f1225f31b8e478e29ed100830d62e5bae185fb2d67d464ae5d4a5ac5e1a3</citedby><cites>FETCH-LOGICAL-c4054-bc4b2f1225f31b8e478e29ed100830d62e5bae185fb2d67d464ae5d4a5ac5e1a3</cites><orcidid>0000-0002-9663-5590</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.202000132$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.202000132$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32885501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deyanova, Ekaterina G.</creatorcontrib><creatorcontrib>Huang, Richard Y.‐C.</creatorcontrib><creatorcontrib>Madia, Priyanka A.</creatorcontrib><creatorcontrib>Nandi, Pradyot</creatorcontrib><creatorcontrib>Gudmundsson, Olafur</creatorcontrib><creatorcontrib>Chen, Guodong</creatorcontrib><title>Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip‐based capillary electrophoresis–mass spectrometry</title><title>Electrophoresis</title><addtitle>Electrophoresis</addtitle><description>Protein glycosylation can impact the efficacy, safety, and pharmacokinetics of therapeutic proteins. Achieving uniform and consistent protein glycosylation is an important requirement for product quality control at all stages of therapeutic protein drug discovery and development. The development of a new microfluidic CE device compatible with MS offers a fast and sensitive orthogonal mode of high‐resolution separation with MS characterization. Here, we describe a fast and robust chip‐based CE‐MS method for intact glycosylation fingerprinting of a therapeutic fusion protein with complex sialylated N and O‐linked glycoforms. The method effectively separates multiple sialylated glycoforms and offers a rapid detection of changes in glycosylation profile in 6 min.</description><subject>CE‐MS</subject><subject>Electrophoresis</subject><subject>Fingerprinting</subject><subject>Glycoprofiling</subject><subject>Mass spectrometry</subject><subject>Microfluidics</subject><subject>Proteins</subject><subject>Quality control</subject><subject>Therapeutic proteins</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKAzEUhoMoWqtblxJwPTXXTroU8QYFxct6yGTOtJHMxWQGmV13bgXf0Ccx2urWVQ7hO__h_xA6omRCCWGn4NowYYQRQihnW2hEJWMJmyq-jUaEpjwhiss9tB_Cc2TETIhdtMeZUlISOkJv97q1BS5tvQDfelt3ccJNiTVe2sXSDXjhBtOEwekOItcH29S49U0Htsb5gCtrfFO63hbWYLO07efqPdchsiYmO6f9gMGB6XzTLhsPwYbP1UelQ8Ch_fmuoPPDAdoptQtwuHnH6Ony4vH8OpnfXt2cn80TI4gUSW5EzkrKmCw5zRWIVAGbQRFdKE6KKQOZa6BKljkrpmkhpkKDLISW2kigmo_RyTo3VnjpIXTZc9P7Op7MmFBKzLiiIlKTNRW7heChzKKaKlbJKMm-vWff3rM_73HheBPb5xUUf_iv6AiINfBqHQz_xGUX87uHlKWCfwGfMJR4</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Deyanova, Ekaterina G.</creator><creator>Huang, Richard Y.‐C.</creator><creator>Madia, Priyanka A.</creator><creator>Nandi, Pradyot</creator><creator>Gudmundsson, Olafur</creator><creator>Chen, Guodong</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-9663-5590</orcidid></search><sort><creationdate>202102</creationdate><title>Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip‐based capillary electrophoresis–mass spectrometry</title><author>Deyanova, Ekaterina G. ; Huang, Richard Y.‐C. ; Madia, Priyanka A. ; Nandi, Pradyot ; Gudmundsson, Olafur ; Chen, Guodong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4054-bc4b2f1225f31b8e478e29ed100830d62e5bae185fb2d67d464ae5d4a5ac5e1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>CE‐MS</topic><topic>Electrophoresis</topic><topic>Fingerprinting</topic><topic>Glycoprofiling</topic><topic>Mass spectrometry</topic><topic>Microfluidics</topic><topic>Proteins</topic><topic>Quality control</topic><topic>Therapeutic proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deyanova, Ekaterina G.</creatorcontrib><creatorcontrib>Huang, Richard Y.‐C.</creatorcontrib><creatorcontrib>Madia, Priyanka A.</creatorcontrib><creatorcontrib>Nandi, Pradyot</creatorcontrib><creatorcontrib>Gudmundsson, Olafur</creatorcontrib><creatorcontrib>Chen, Guodong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deyanova, Ekaterina G.</au><au>Huang, Richard Y.‐C.</au><au>Madia, Priyanka A.</au><au>Nandi, Pradyot</au><au>Gudmundsson, Olafur</au><au>Chen, Guodong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip‐based capillary electrophoresis–mass spectrometry</atitle><jtitle>Electrophoresis</jtitle><addtitle>Electrophoresis</addtitle><date>2021-02</date><risdate>2021</risdate><volume>42</volume><issue>4</issue><spage>460</spage><epage>464</epage><pages>460-464</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Protein glycosylation can impact the efficacy, safety, and pharmacokinetics of therapeutic proteins. Achieving uniform and consistent protein glycosylation is an important requirement for product quality control at all stages of therapeutic protein drug discovery and development. The development of a new microfluidic CE device compatible with MS offers a fast and sensitive orthogonal mode of high‐resolution separation with MS characterization. Here, we describe a fast and robust chip‐based CE‐MS method for intact glycosylation fingerprinting of a therapeutic fusion protein with complex sialylated N and O‐linked glycoforms. The method effectively separates multiple sialylated glycoforms and offers a rapid detection of changes in glycosylation profile in 6 min.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32885501</pmid><doi>10.1002/elps.202000132</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9663-5590</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0173-0835 |
| ispartof | Electrophoresis, 2021-02, Vol.42 (4), p.460-464 |
| issn | 0173-0835 1522-2683 |
| language | eng |
| recordid | cdi_proquest_journals_2488493814 |
| source | Wiley Online Library All Journals |
| subjects | CE‐MS Electrophoresis Fingerprinting Glycoprofiling Mass spectrometry Microfluidics Proteins Quality control Therapeutic proteins |
| title | Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip‐based capillary electrophoresis–mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T22%3A35%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20fingerprinting%20of%20a%20highly%20glycosylated%20fusion%20protein%20by%20microfluidic%20chip%E2%80%90based%20capillary%20electrophoresis%E2%80%93mass%20spectrometry&rft.jtitle=Electrophoresis&rft.au=Deyanova,%20Ekaterina%20G.&rft.date=2021-02&rft.volume=42&rft.issue=4&rft.spage=460&rft.epage=464&rft.pages=460-464&rft.issn=0173-0835&rft.eissn=1522-2683&rft_id=info:doi/10.1002/elps.202000132&rft_dat=%3Cproquest_cross%3E2488493814%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2488493814&rft_id=info:pmid/32885501&rfr_iscdi=true |