A DNA based visual and colorimetric aggregation assay for the early growth factor receptor (EGFR) mutation by using unmodified gold nanoparticles
A genomic DNA-based colorimetric assay is described for the detection of the early growth factor receptor (EGFR) mutation, which is the protruding reason for non-small cell lung cancer. A DNA sequence was designed and immobilized on unmodified gold nanoparticles (GNPs). The formation of the respecti...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2019-08, Vol.186 (8), p.546, Article 546 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Ramanathan, Santheraleka Gopinath, Subash C. B. Arshad, M. K. Md Poopalan, Prabakaran Anbu, Periasamy |
| description | A genomic DNA-based colorimetric assay is described for the detection of the early growth factor receptor (EGFR) mutation, which is the protruding reason for non-small cell lung cancer. A DNA sequence was designed and immobilized on unmodified gold nanoparticles (GNPs). The formation of the respective duplex indicates the presence of an EGFR mutation. It is accompanied by the aggregation of the GNPs in the presence of monovalent ions, and it indicates the presence of an EGFR mutation. This is accompanied by a color change from red (520 nm) to purple (620 nm). Aggregation was evidenced by transmission electron microscopy, scanning electron microscopy and atomic force microscopy. The limit of detection is 313 nM of the mutant target strand. A similar peak shift was observed for 2.5 μM concentrations of wild type target. No significant peak shift was observed with probe and non-complementary DNA.
Graphical abstract
Schematic representation of high-specific genomic DNA sequence on gold nanoparticle (GNP) aggregation with sodium chloride (NaCl). It illustrates the detection method for EGFR mutation on lung cancer detection. Red and purple colors of tubes represent dispersed and aggregated GNP, respectively. |
| doi_str_mv | 10.1007/s00604-019-3696-y |
| format | Article |
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Graphical abstract
Schematic representation of high-specific genomic DNA sequence on gold nanoparticle (GNP) aggregation with sodium chloride (NaCl). It illustrates the detection method for EGFR mutation on lung cancer detection. Red and purple colors of tubes represent dispersed and aggregated GNP, respectively.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-019-3696-y</identifier><identifier>PMID: 31321546</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Agglomeration ; Analytical Chemistry ; Atomic force microscopy ; Cancer ; Carcinoma, Non-Small-Cell Lung - genetics ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Colorimetry ; Deoxyribonucleic acid ; DNA ; DNA sequencing ; DNA, Single-Stranded - chemistry ; Electron microscopy ; ErbB Receptors - genetics ; Genetic aspects ; Genetic research ; Gold ; Gold - chemistry ; Growth factors ; Lung cancer ; Lung cancer, Non-small cell ; Lung Neoplasms - genetics ; Metal Nanoparticles - chemistry ; Microengineering ; Microscopy ; Mutation ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Nucleotide sequencing ; Original Paper ; Receptors</subject><ispartof>Mikrochimica acta (1966), 2019-08, Vol.186 (8), p.546, Article 546</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-8e21bd1ac2060411cd81d68e15e5863af5cee730fc80452927379945914a69173</citedby><cites>FETCH-LOGICAL-c439t-8e21bd1ac2060411cd81d68e15e5863af5cee730fc80452927379945914a69173</cites><orcidid>0000-0002-8347-4687</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-019-3696-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-019-3696-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31321546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramanathan, Santheraleka</creatorcontrib><creatorcontrib>Gopinath, Subash C. B.</creatorcontrib><creatorcontrib>Arshad, M. K. Md</creatorcontrib><creatorcontrib>Poopalan, Prabakaran</creatorcontrib><creatorcontrib>Anbu, Periasamy</creatorcontrib><title>A DNA based visual and colorimetric aggregation assay for the early growth factor receptor (EGFR) mutation by using unmodified gold nanoparticles</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A genomic DNA-based colorimetric assay is described for the detection of the early growth factor receptor (EGFR) mutation, which is the protruding reason for non-small cell lung cancer. A DNA sequence was designed and immobilized on unmodified gold nanoparticles (GNPs). The formation of the respective duplex indicates the presence of an EGFR mutation. It is accompanied by the aggregation of the GNPs in the presence of monovalent ions, and it indicates the presence of an EGFR mutation. This is accompanied by a color change from red (520 nm) to purple (620 nm). Aggregation was evidenced by transmission electron microscopy, scanning electron microscopy and atomic force microscopy. The limit of detection is 313 nM of the mutant target strand. A similar peak shift was observed for 2.5 μM concentrations of wild type target. No significant peak shift was observed with probe and non-complementary DNA.
Graphical abstract
Schematic representation of high-specific genomic DNA sequence on gold nanoparticle (GNP) aggregation with sodium chloride (NaCl). It illustrates the detection method for EGFR mutation on lung cancer detection. Red and purple colors of tubes represent dispersed and aggregated GNP, respectively.</description><subject>Agglomeration</subject><subject>Analytical Chemistry</subject><subject>Atomic force microscopy</subject><subject>Cancer</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colorimetry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA sequencing</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Electron microscopy</subject><subject>ErbB Receptors - genetics</subject><subject>Genetic aspects</subject><subject>Genetic research</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Growth factors</subject><subject>Lung cancer</subject><subject>Lung cancer, Non-small cell</subject><subject>Lung Neoplasms - genetics</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microengineering</subject><subject>Microscopy</subject><subject>Mutation</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nucleotide sequencing</subject><subject>Original Paper</subject><subject>Receptors</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UU1v1DAQtRAVXQo_gAuyxAUOaf0R28lxVdqCVLUSgrPldSapq8RebKcoP4N_jFcpVEigOXg089688TyE3lBySglRZ4kQSeqK0LbispXV8gxtaM1lJYjiz9GGECZLR7Fj9DKle0Kokqx-gY455YyKWm7Qzy3-eLPFO5Ogww8uzWbExnfYhjFEN0GOzmIzDBEGk13w2KRkFtyHiPMdYDBxXPAQw498h3tjc6lHsLA_JO8vri6_fMDTnFfqbsFzcn7As59C53pXJIcwdtgbH_YmZmdHSK_QUW_GBK8f3xP07fLi6_mn6vr26vP59rqyNW9z1QCju44ayw43oNR2De1kA1SAaCQ3vbAAipPeNqQWrGWKq7atRUtrI1uq-Al6t87dx_B9hpT1fZijL5Ka1U1DSJFhT6jBjKCd70OOxk4uWb1VVBChmOQFdfoPVIkOJmeDh96V-l8EuhJsDClF6PW-XNvERVOiD97q1VtdvNUHb_VSOG8fF553E3R_GL_NLAC2AlJp-QHi04_-P_UXlfGuVA</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Ramanathan, Santheraleka</creator><creator>Gopinath, Subash C. B.</creator><creator>Arshad, M. K. Md</creator><creator>Poopalan, Prabakaran</creator><creator>Anbu, Periasamy</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-8347-4687</orcidid></search><sort><creationdate>20190801</creationdate><title>A DNA based visual and colorimetric aggregation assay for the early growth factor receptor (EGFR) mutation by using unmodified gold nanoparticles</title><author>Ramanathan, Santheraleka ; Gopinath, Subash C. B. ; Arshad, M. K. Md ; Poopalan, Prabakaran ; Anbu, Periasamy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-8e21bd1ac2060411cd81d68e15e5863af5cee730fc80452927379945914a69173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agglomeration</topic><topic>Analytical Chemistry</topic><topic>Atomic force microscopy</topic><topic>Cancer</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colorimetry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA sequencing</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Electron microscopy</topic><topic>ErbB Receptors - genetics</topic><topic>Genetic aspects</topic><topic>Genetic research</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Growth factors</topic><topic>Lung cancer</topic><topic>Lung cancer, Non-small cell</topic><topic>Lung Neoplasms - genetics</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microengineering</topic><topic>Microscopy</topic><topic>Mutation</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nucleotide sequencing</topic><topic>Original Paper</topic><topic>Receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramanathan, Santheraleka</creatorcontrib><creatorcontrib>Gopinath, Subash C. 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Md</au><au>Poopalan, Prabakaran</au><au>Anbu, Periasamy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A DNA based visual and colorimetric aggregation assay for the early growth factor receptor (EGFR) mutation by using unmodified gold nanoparticles</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>186</volume><issue>8</issue><spage>546</spage><pages>546-</pages><artnum>546</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A genomic DNA-based colorimetric assay is described for the detection of the early growth factor receptor (EGFR) mutation, which is the protruding reason for non-small cell lung cancer. A DNA sequence was designed and immobilized on unmodified gold nanoparticles (GNPs). The formation of the respective duplex indicates the presence of an EGFR mutation. It is accompanied by the aggregation of the GNPs in the presence of monovalent ions, and it indicates the presence of an EGFR mutation. This is accompanied by a color change from red (520 nm) to purple (620 nm). Aggregation was evidenced by transmission electron microscopy, scanning electron microscopy and atomic force microscopy. The limit of detection is 313 nM of the mutant target strand. A similar peak shift was observed for 2.5 μM concentrations of wild type target. No significant peak shift was observed with probe and non-complementary DNA.
Graphical abstract
Schematic representation of high-specific genomic DNA sequence on gold nanoparticle (GNP) aggregation with sodium chloride (NaCl). It illustrates the detection method for EGFR mutation on lung cancer detection. Red and purple colors of tubes represent dispersed and aggregated GNP, respectively.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>31321546</pmid><doi>10.1007/s00604-019-3696-y</doi><orcidid>https://orcid.org/0000-0002-8347-4687</orcidid></addata></record> |
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| subjects | Agglomeration Analytical Chemistry Atomic force microscopy Cancer Carcinoma, Non-Small-Cell Lung - genetics Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Colorimetry Deoxyribonucleic acid DNA DNA sequencing DNA, Single-Stranded - chemistry Electron microscopy ErbB Receptors - genetics Genetic aspects Genetic research Gold Gold - chemistry Growth factors Lung cancer Lung cancer, Non-small cell Lung Neoplasms - genetics Metal Nanoparticles - chemistry Microengineering Microscopy Mutation Nanochemistry Nanoparticles Nanotechnology Nucleotide sequencing Original Paper Receptors |
| title | A DNA based visual and colorimetric aggregation assay for the early growth factor receptor (EGFR) mutation by using unmodified gold nanoparticles |
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