Phytochemical Investigation and Antioxidant Activity of Globularia alypum L
The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. co...
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| Veröffentlicht in: | Molecules (Basel, Switzerland) Switzerland), 2021-02, Vol.26 (3), p.759, Article 759 |
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| creator | Asraoui, Fadoua Kounnoun, Ayoub El Cadi, Hafssa Cacciola, Francesco El Majdoub, Yassine Oulad Alibrando, Filippo Mandolfino, Filippo Dugo, Paola Mondello, Luigi Louajri, Adnane |
| description | The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 +/- 0.61 mu g GAE/mg of extract in comparison with the chloroform extract (18.9 +/- 0.48 mu g GAE/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 +/- 0.55 mu g CE/mg of extract) in comparison with the chloroform extract (18.0 +/- 0.36 mu g CE/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC50 = 12.3 +/- 3.83 mu g/mL and IC50 = 37.0 +/- 2.45 mu g/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 +/- 17.08 (mg AAE/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them n-hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants. |
| doi_str_mv | 10.3390/molecules26030759 |
| format | Article |
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The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 +/- 0.61 mu g GAE/mg of extract in comparison with the chloroform extract (18.9 +/- 0.48 mu g GAE/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 +/- 0.55 mu g CE/mg of extract) in comparison with the chloroform extract (18.0 +/- 0.36 mu g CE/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC50 = 12.3 +/- 3.83 mu g/mL and IC50 = 37.0 +/- 2.45 mu g/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 +/- 17.08 (mg AAE/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them n-hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules26030759</identifier><identifier>PMID: 33540622</identifier><language>eng</language><publisher>BASEL: Mdpi</publisher><subject>Acetic acid ; Acids ; Allelochemicals ; antioxidant activity ; Antioxidants ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Assaying ; Biochemistry & Molecular Biology ; Chemical composition ; Chemistry ; Chemistry, Multidisciplinary ; Chloroform ; Chromatography ; Ethyl acetate ; Flavonoids ; Flavonoids - analysis ; Free radicals ; GC-MS ; Globularia alypum L ; Herbal medicine ; High-performance liquid chromatography ; HPLC-DAD/ESI-MS ; Leaves ; Life Sciences & Biomedicine ; Liquid chromatography ; Medical treatment ; Medicinal plants ; Metabolites ; Palmitic acid ; Phenolic compounds ; Phenols ; Phenols - analysis ; Physical Sciences ; Phytochemicals ; Phytochemicals - chemistry ; Phytochemicals - pharmacology ; Plant extracts ; Plantaginaceae - chemistry ; Plants (botany) ; Science & Technology ; Solvents - chemistry ; Volatile compounds ; Volatiles</subject><ispartof>Molecules (Basel, Switzerland), 2021-02, Vol.26 (3), p.759, Article 759</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>32</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000615429500001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c493t-811760c876b56f19fdeac63c95e5bbf5a14bed00a31fa66413f566e293b9e9b93</citedby><cites>FETCH-LOGICAL-c493t-811760c876b56f19fdeac63c95e5bbf5a14bed00a31fa66413f566e293b9e9b93</cites><orcidid>0000-0002-0370-5482 ; 0000-0002-8890-675X ; 0000-0003-1296-7633 ; 0000-0002-3318-7073</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867259/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867259/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2115,27929,27930,39263,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33540622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asraoui, Fadoua</creatorcontrib><creatorcontrib>Kounnoun, Ayoub</creatorcontrib><creatorcontrib>El Cadi, Hafssa</creatorcontrib><creatorcontrib>Cacciola, Francesco</creatorcontrib><creatorcontrib>El Majdoub, Yassine Oulad</creatorcontrib><creatorcontrib>Alibrando, Filippo</creatorcontrib><creatorcontrib>Mandolfino, Filippo</creatorcontrib><creatorcontrib>Dugo, Paola</creatorcontrib><creatorcontrib>Mondello, Luigi</creatorcontrib><creatorcontrib>Louajri, Adnane</creatorcontrib><title>Phytochemical Investigation and Antioxidant Activity of Globularia alypum L</title><title>Molecules (Basel, Switzerland)</title><addtitle>MOLECULES</addtitle><addtitle>Molecules</addtitle><description>The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 +/- 0.61 mu g GAE/mg of extract in comparison with the chloroform extract (18.9 +/- 0.48 mu g GAE/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 +/- 0.55 mu g CE/mg of extract) in comparison with the chloroform extract (18.0 +/- 0.36 mu g CE/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC50 = 12.3 +/- 3.83 mu g/mL and IC50 = 37.0 +/- 2.45 mu g/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 +/- 17.08 (mg AAE/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them n-hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants.</description><subject>Acetic acid</subject><subject>Acids</subject><subject>Allelochemicals</subject><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Assaying</subject><subject>Biochemistry & Molecular Biology</subject><subject>Chemical composition</subject><subject>Chemistry</subject><subject>Chemistry, Multidisciplinary</subject><subject>Chloroform</subject><subject>Chromatography</subject><subject>Ethyl acetate</subject><subject>Flavonoids</subject><subject>Flavonoids - analysis</subject><subject>Free radicals</subject><subject>GC-MS</subject><subject>Globularia alypum L</subject><subject>Herbal medicine</subject><subject>High-performance liquid chromatography</subject><subject>HPLC-DAD/ESI-MS</subject><subject>Leaves</subject><subject>Life Sciences & Biomedicine</subject><subject>Liquid chromatography</subject><subject>Medical treatment</subject><subject>Medicinal plants</subject><subject>Metabolites</subject><subject>Palmitic acid</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Phenols - analysis</subject><subject>Physical Sciences</subject><subject>Phytochemicals</subject><subject>Phytochemicals - chemistry</subject><subject>Phytochemicals - pharmacology</subject><subject>Plant extracts</subject><subject>Plantaginaceae - chemistry</subject><subject>Plants (botany)</subject><subject>Science & Technology</subject><subject>Solvents - chemistry</subject><subject>Volatile compounds</subject><subject>Volatiles</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNqNkUtvEzEUhUcIREvhB7BBI7FBQgG_Z7xBiiIoEZFgAWvLj-vE0YwdZjwp-fe4SYla2LDykf2do-t7quolRu8oleh9nzqwUwcjEYiihstH1SVmBM0oYvLxPX1RPRvHLUIEM8yfVheUcoYEIZfVl2-bQ052A32wuquXcQ9jDmudQ4q1jq6exyJ_Badjruc2h33Ihzr5-rpLZur0EHStu8Nu6uvV8-qJ190IL-7Oq-rHp4_fF59nq6_Xy8V8NbNM0jxrMW4Esm0jDBceS-9AW0Gt5MCN8VxjZsAhpCn2WgiGqedCAJHUSJBG0qtqecp1SW_Vbgi9Hg4q6aCOF2lYKz3kYDtQzjnmPGYA0DLZ2tbhBjTHFLXGE-NL1odT1m4yPTgLMQ-6exD68CWGjVqnvWpa0RB-O8ybu4Ah_ZzK8lQfRgtdpyOkaVSEtQ3mRGJR0Nd_ods0DbGs6kihFpeqCoVPlB3SOA7gz8NgpG5rV__UXjyv7v_i7PjTcwHaE3ADJvnRBogWzhhCSGDOiORFIbwI-dj_Ik0xF-vb_7fS37ifzP8</recordid><startdate>20210202</startdate><enddate>20210202</enddate><creator>Asraoui, Fadoua</creator><creator>Kounnoun, Ayoub</creator><creator>El Cadi, Hafssa</creator><creator>Cacciola, Francesco</creator><creator>El Majdoub, Yassine Oulad</creator><creator>Alibrando, Filippo</creator><creator>Mandolfino, Filippo</creator><creator>Dugo, Paola</creator><creator>Mondello, Luigi</creator><creator>Louajri, Adnane</creator><general>Mdpi</general><general>MDPI AG</general><general>MDPI</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0370-5482</orcidid><orcidid>https://orcid.org/0000-0002-8890-675X</orcidid><orcidid>https://orcid.org/0000-0003-1296-7633</orcidid><orcidid>https://orcid.org/0000-0002-3318-7073</orcidid></search><sort><creationdate>20210202</creationdate><title>Phytochemical Investigation and Antioxidant Activity of Globularia alypum L</title><author>Asraoui, Fadoua ; Kounnoun, Ayoub ; El Cadi, Hafssa ; Cacciola, Francesco ; El Majdoub, Yassine Oulad ; Alibrando, Filippo ; Mandolfino, Filippo ; Dugo, Paola ; Mondello, Luigi ; Louajri, Adnane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-811760c876b56f19fdeac63c95e5bbf5a14bed00a31fa66413f566e293b9e9b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acetic acid</topic><topic>Acids</topic><topic>Allelochemicals</topic><topic>antioxidant activity</topic><topic>Antioxidants</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - pharmacology</topic><topic>Assaying</topic><topic>Biochemistry & Molecular Biology</topic><topic>Chemical composition</topic><topic>Chemistry</topic><topic>Chemistry, Multidisciplinary</topic><topic>Chloroform</topic><topic>Chromatography</topic><topic>Ethyl acetate</topic><topic>Flavonoids</topic><topic>Flavonoids - analysis</topic><topic>Free radicals</topic><topic>GC-MS</topic><topic>Globularia alypum L</topic><topic>Herbal medicine</topic><topic>High-performance liquid chromatography</topic><topic>HPLC-DAD/ESI-MS</topic><topic>Leaves</topic><topic>Life Sciences & Biomedicine</topic><topic>Liquid chromatography</topic><topic>Medical treatment</topic><topic>Medicinal plants</topic><topic>Metabolites</topic><topic>Palmitic acid</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Phenols - analysis</topic><topic>Physical Sciences</topic><topic>Phytochemicals</topic><topic>Phytochemicals - chemistry</topic><topic>Phytochemicals - pharmacology</topic><topic>Plant extracts</topic><topic>Plantaginaceae - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asraoui, Fadoua</au><au>Kounnoun, Ayoub</au><au>El Cadi, Hafssa</au><au>Cacciola, Francesco</au><au>El Majdoub, Yassine Oulad</au><au>Alibrando, Filippo</au><au>Mandolfino, Filippo</au><au>Dugo, Paola</au><au>Mondello, Luigi</au><au>Louajri, Adnane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical Investigation and Antioxidant Activity of Globularia alypum L</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><stitle>MOLECULES</stitle><addtitle>Molecules</addtitle><date>2021-02-02</date><risdate>2021</risdate><volume>26</volume><issue>3</issue><spage>759</spage><pages>759-</pages><artnum>759</artnum><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from Globularia alypum L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 +/- 0.61 mu g GAE/mg of extract in comparison with the chloroform extract (18.9 +/- 0.48 mu g GAE/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 +/- 0.55 mu g CE/mg of extract) in comparison with the chloroform extract (18.0 +/- 0.36 mu g CE/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC50 = 12.3 +/- 3.83 mu g/mL and IC50 = 37.0 +/- 2.45 mu g/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 +/- 17.08 (mg AAE/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them n-hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants.</abstract><cop>BASEL</cop><pub>Mdpi</pub><pmid>33540622</pmid><doi>10.3390/molecules26030759</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0370-5482</orcidid><orcidid>https://orcid.org/0000-0002-8890-675X</orcidid><orcidid>https://orcid.org/0000-0003-1296-7633</orcidid><orcidid>https://orcid.org/0000-0002-3318-7073</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetic acid Acids Allelochemicals antioxidant activity Antioxidants Antioxidants - chemistry Antioxidants - pharmacology Assaying Biochemistry & Molecular Biology Chemical composition Chemistry Chemistry, Multidisciplinary Chloroform Chromatography Ethyl acetate Flavonoids Flavonoids - analysis Free radicals GC-MS Globularia alypum L Herbal medicine High-performance liquid chromatography HPLC-DAD/ESI-MS Leaves Life Sciences & Biomedicine Liquid chromatography Medical treatment Medicinal plants Metabolites Palmitic acid Phenolic compounds Phenols Phenols - analysis Physical Sciences Phytochemicals Phytochemicals - chemistry Phytochemicals - pharmacology Plant extracts Plantaginaceae - chemistry Plants (botany) Science & Technology Solvents - chemistry Volatile compounds Volatiles |
| title | Phytochemical Investigation and Antioxidant Activity of Globularia alypum L |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T10%3A53%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_webof&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phytochemical%20Investigation%20and%20Antioxidant%20Activity%20of%20Globularia%20alypum%20L&rft.jtitle=Molecules%20(Basel,%20Switzerland)&rft.au=Asraoui,%20Fadoua&rft.date=2021-02-02&rft.volume=26&rft.issue=3&rft.spage=759&rft.pages=759-&rft.artnum=759&rft.issn=1420-3049&rft.eissn=1420-3049&rft_id=info:doi/10.3390/molecules26030759&rft_dat=%3Cproquest_webof%3E2487081049%3C/proquest_webof%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2487081049&rft_id=info:pmid/33540622&rft_doaj_id=oai_doaj_org_article_ddd4df14eee8498c8d17ea51308bf2bf&rfr_iscdi=true |