Thymol‐Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
Thymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol‐loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxi...
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| Veröffentlicht in: | Environmental toxicology and chemistry 2021-02, Vol.40 (2), p.333-341 |
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| creator | Pereira, Iúri Barbosa Carvalho, Endrew Henrique de Sousa Rodrigues, Laís de Brito Mattos, Bruno Dufau Magalhães, Washington Luiz Esteves Leme, Daniela Morais Krawczyk‐Santos, Anna Paula Taveira, Stephânia Fleury Oliveira, Gisele Augusto Rodrigues |
| description | Thymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol‐loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxicogenetic effects induced by thymol, BSiO2 NP, and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early life stages. We also investigated the impact of BSiO2 aggregation in different exposure media (saline and freshwater). Based on the median lethal concentration at 48 h (LC5048h), BSiO2#THY (LC5048h = 1.06 mg/L) presented similar toxic potential as thymol (LC5048h = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. Because BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of thymol. For zebrafish early life stages, BSiO2#THY (LC5096h = 13.13 mg/L) was more toxic than free thymol (LC5096h = 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced thymol's availability for this aquatic organism. Also, BSiO2#THY significantly induced sublethal effects as thymol, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. Environ Toxicol Chem 2021;40:333–341. © 2020 SETAC
Toxicity of BSiO2#THY to Artemia salina and zebrafish (Danio rerio) early life stages and the aggregation impacts on different exposure media of the BSiO2 nanocarrier. BSiO2 = biogenic silica nanoparticles; BSiO2#THY = THY‐loaded BSiO2; THY = thymol. |
| doi_str_mv | 10.1002/etc.4938 |
| format | Article |
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Toxicity of BSiO2#THY to Artemia salina and zebrafish (Danio rerio) early life stages and the aggregation impacts on different exposure media of the BSiO2 nanocarrier. BSiO2 = biogenic silica nanoparticles; BSiO2#THY = THY‐loaded BSiO2; THY = thymol.</description><identifier>ISSN: 0730-7268</identifier><identifier>EISSN: 1552-8618</identifier><identifier>DOI: 10.1002/etc.4938</identifier><identifier>PMID: 33210755</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Agglomeration ; Aggregation ; Aquatic ecosystems ; Aquatic environment ; Aquatic organisms ; Aqueous solutions ; Artemia ; Artemia salina ; Biocide delivery systems ; Biocides ; Comet assay ; Danio rerio ; Environmental impact ; Freshwater ecosystems ; Genotoxicity ; Nanoparticles ; Phenols ; Silica ; Silicon dioxide ; Sublethal effects ; Thymol ; Toxicity ; Zebrafish</subject><ispartof>Environmental toxicology and chemistry, 2021-02, Vol.40 (2), p.333-341</ispartof><rights>2020 SETAC</rights><rights>2020 SETAC.</rights><rights>2021 SETAC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4028-7cb13d52a87a956734d265041393d73d87d2f3837f3de1c75db5b14750f281f93</citedby><cites>FETCH-LOGICAL-c4028-7cb13d52a87a956734d265041393d73d87d2f3837f3de1c75db5b14750f281f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fetc.4938$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fetc.4938$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33210755$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pereira, Iúri Barbosa</creatorcontrib><creatorcontrib>Carvalho, Endrew Henrique de Sousa</creatorcontrib><creatorcontrib>Rodrigues, Laís de Brito</creatorcontrib><creatorcontrib>Mattos, Bruno Dufau</creatorcontrib><creatorcontrib>Magalhães, Washington Luiz Esteves</creatorcontrib><creatorcontrib>Leme, Daniela Morais</creatorcontrib><creatorcontrib>Krawczyk‐Santos, Anna Paula</creatorcontrib><creatorcontrib>Taveira, Stephânia Fleury</creatorcontrib><creatorcontrib>Oliveira, Gisele Augusto Rodrigues</creatorcontrib><title>Thymol‐Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity</title><title>Environmental toxicology and chemistry</title><addtitle>Environ Toxicol Chem</addtitle><description>Thymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol‐loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxicogenetic effects induced by thymol, BSiO2 NP, and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early life stages. We also investigated the impact of BSiO2 aggregation in different exposure media (saline and freshwater). Based on the median lethal concentration at 48 h (LC5048h), BSiO2#THY (LC5048h = 1.06 mg/L) presented similar toxic potential as thymol (LC5048h = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. Because BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of thymol. For zebrafish early life stages, BSiO2#THY (LC5096h = 13.13 mg/L) was more toxic than free thymol (LC5096h = 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced thymol's availability for this aquatic organism. Also, BSiO2#THY significantly induced sublethal effects as thymol, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. Environ Toxicol Chem 2021;40:333–341. © 2020 SETAC
Toxicity of BSiO2#THY to Artemia salina and zebrafish (Danio rerio) early life stages and the aggregation impacts on different exposure media of the BSiO2 nanocarrier. BSiO2 = biogenic silica nanoparticles; BSiO2#THY = THY‐loaded BSiO2; THY = thymol.</description><subject>Agglomeration</subject><subject>Aggregation</subject><subject>Aquatic ecosystems</subject><subject>Aquatic environment</subject><subject>Aquatic organisms</subject><subject>Aqueous solutions</subject><subject>Artemia</subject><subject>Artemia salina</subject><subject>Biocide delivery systems</subject><subject>Biocides</subject><subject>Comet assay</subject><subject>Danio rerio</subject><subject>Environmental impact</subject><subject>Freshwater ecosystems</subject><subject>Genotoxicity</subject><subject>Nanoparticles</subject><subject>Phenols</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Sublethal effects</subject><subject>Thymol</subject><subject>Toxicity</subject><subject>Zebrafish</subject><issn>0730-7268</issn><issn>1552-8618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kMtKAzEUQIMoWh_gF0jAjZupeUwmGXe1VC0UFazrIU0yNWUmaTNTta78BL_RLzHa6k4IXAjnngsHgGOMuhghcm5a1U1zKrZABzNGEpFhsQ06iFOUcJKJPbDfNDOEcJbn-S7Yo5RgxBnrgLfx06r21ef7x8hLbTS8tH5qnFXwwVZWSXgrnZ_L0FpVmQZaB6WDvcVSxg84cM82eFcb117A8ZOBw3ouVQt9Ce83K7A3nQYzjbh3ML6B8q1_tcq2q0OwU8qqMUebeQAerwbj_k0yurse9nujRKWIiISrCaaaESm4zFnGaapJxlCKaU41p1pwTUoqKC-pNlhxpidsglPOUEkELnN6AE7X3nnwi6Vp2mLml8HFkwVJBSIpSkUWqbM1pYJvmmDKYh5sLcOqwKj4jlzEyMV35IiebITLSW30H_hbNQLJGnixlVn9Kyoi8yP8AplvhkE</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Pereira, Iúri Barbosa</creator><creator>Carvalho, Endrew Henrique de Sousa</creator><creator>Rodrigues, Laís de Brito</creator><creator>Mattos, Bruno Dufau</creator><creator>Magalhães, Washington Luiz Esteves</creator><creator>Leme, Daniela Morais</creator><creator>Krawczyk‐Santos, Anna Paula</creator><creator>Taveira, Stephânia Fleury</creator><creator>Oliveira, Gisele Augusto Rodrigues</creator><general>Blackwell Publishing Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>202102</creationdate><title>Thymol‐Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity</title><author>Pereira, Iúri Barbosa ; Carvalho, Endrew Henrique de Sousa ; Rodrigues, Laís de Brito ; Mattos, Bruno Dufau ; Magalhães, Washington Luiz Esteves ; Leme, Daniela Morais ; Krawczyk‐Santos, Anna Paula ; Taveira, Stephânia Fleury ; Oliveira, Gisele Augusto Rodrigues</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4028-7cb13d52a87a956734d265041393d73d87d2f3837f3de1c75db5b14750f281f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agglomeration</topic><topic>Aggregation</topic><topic>Aquatic ecosystems</topic><topic>Aquatic environment</topic><topic>Aquatic organisms</topic><topic>Aqueous solutions</topic><topic>Artemia</topic><topic>Artemia salina</topic><topic>Biocide delivery systems</topic><topic>Biocides</topic><topic>Comet assay</topic><topic>Danio rerio</topic><topic>Environmental impact</topic><topic>Freshwater ecosystems</topic><topic>Genotoxicity</topic><topic>Nanoparticles</topic><topic>Phenols</topic><topic>Silica</topic><topic>Silicon dioxide</topic><topic>Sublethal effects</topic><topic>Thymol</topic><topic>Toxicity</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pereira, Iúri Barbosa</creatorcontrib><creatorcontrib>Carvalho, Endrew Henrique de Sousa</creatorcontrib><creatorcontrib>Rodrigues, Laís de Brito</creatorcontrib><creatorcontrib>Mattos, Bruno Dufau</creatorcontrib><creatorcontrib>Magalhães, Washington Luiz Esteves</creatorcontrib><creatorcontrib>Leme, Daniela Morais</creatorcontrib><creatorcontrib>Krawczyk‐Santos, Anna Paula</creatorcontrib><creatorcontrib>Taveira, Stephânia Fleury</creatorcontrib><creatorcontrib>Oliveira, Gisele Augusto Rodrigues</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental toxicology and chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, Iúri Barbosa</au><au>Carvalho, Endrew Henrique de Sousa</au><au>Rodrigues, Laís de Brito</au><au>Mattos, Bruno Dufau</au><au>Magalhães, Washington Luiz Esteves</au><au>Leme, Daniela Morais</au><au>Krawczyk‐Santos, Anna Paula</au><au>Taveira, Stephânia Fleury</au><au>Oliveira, Gisele Augusto Rodrigues</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thymol‐Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity</atitle><jtitle>Environmental toxicology and chemistry</jtitle><addtitle>Environ Toxicol Chem</addtitle><date>2021-02</date><risdate>2021</risdate><volume>40</volume><issue>2</issue><spage>333</spage><epage>341</epage><pages>333-341</pages><issn>0730-7268</issn><eissn>1552-8618</eissn><abstract>Thymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol‐loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxicogenetic effects induced by thymol, BSiO2 NP, and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early life stages. We also investigated the impact of BSiO2 aggregation in different exposure media (saline and freshwater). Based on the median lethal concentration at 48 h (LC5048h), BSiO2#THY (LC5048h = 1.06 mg/L) presented similar toxic potential as thymol (LC5048h = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. Because BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of thymol. For zebrafish early life stages, BSiO2#THY (LC5096h = 13.13 mg/L) was more toxic than free thymol (LC5096h = 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced thymol's availability for this aquatic organism. Also, BSiO2#THY significantly induced sublethal effects as thymol, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. Environ Toxicol Chem 2021;40:333–341. © 2020 SETAC
Toxicity of BSiO2#THY to Artemia salina and zebrafish (Danio rerio) early life stages and the aggregation impacts on different exposure media of the BSiO2 nanocarrier. BSiO2 = biogenic silica nanoparticles; BSiO2#THY = THY‐loaded BSiO2; THY = thymol.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>33210755</pmid><doi>10.1002/etc.4938</doi><tpages>8</tpages></addata></record> |
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| ispartof | Environmental toxicology and chemistry, 2021-02, Vol.40 (2), p.333-341 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Agglomeration Aggregation Aquatic ecosystems Aquatic environment Aquatic organisms Aqueous solutions Artemia Artemia salina Biocide delivery systems Biocides Comet assay Danio rerio Environmental impact Freshwater ecosystems Genotoxicity Nanoparticles Phenols Silica Silicon dioxide Sublethal effects Thymol Toxicity Zebrafish |
| title | Thymol‐Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity |
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