Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract
Background/Aim Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues. Materials and Met...
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| Veröffentlicht in: | Dental traumatology 2021-02, Vol.37 (1), p.123-130 |
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| container_title | Dental traumatology |
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| creator | Bunwanna, Atittaya Damrongrungruang, Teerasak Puasiri, Subin Kantrong, Nutthapong Chailertvanitkul, Pattama |
| description | Background/Aim
Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues.
Materials and Methods
PDL cells from human premolars were tested for cytotoxicity of the extract by PrestoBlue assay to determine a non‐toxic concentration. Subsequently, 96 freshly extracted premolars were allocated into different treatment groups. Control groups were freshly extracted premolars or they had been stored dry for 12 hours. Experimental avulsed teeth were created by leaving them air‐dried for 30 minutes immediately after extraction, then they were immersed in Thai propolis extract, HBSS or milk for 3, 6 and 12 hours. After tooth storage, the remaining PDL cells were determined for their cell viability. RNA isolated from PDL tissues of three premolars treated similarly was analysed for periostin and S100A4 expressions using RT‐qPCR.
Results
Thai propolis extract at 0.625 mg mL−1 promoted the greatest PDL cell viability. Tooth storage in 0.625 mg mL−1 Thai propolis extract, HBSS or milk showed no difference in maintaining cell viability. Periostin mRNA level was preserved by Thai propolis extract. Expression of S100A4 mRNA in PDL tissues stored in all tested media was dampened.
Conclusions
PDL cells from mock avulsed teeth stored in 0.625 mg mL−1 Thai propolis extract for 3, 6 and 12 hours remained viable and the expression of periostin was preserved. This study suggests this extract as an alternative for a tooth storage medium for up to 12 hours. However, transporting an avulsed tooth in a storage medium for extended extra‐oral time might affect the PDL cell phenotypes. |
| doi_str_mv | 10.1111/edt.12612 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2478719647</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2478719647</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3532-e7c8a9f00ef488e0464a6090b45313b0a9f41bae4b5804a53acea5c28c52689d3</originalsourceid><addsrcrecordid>eNp1kD1PwzAQhi0EoqUw8AeQJSaGtHbiOMmIoHxIlWAos-Ukl9ZVEgfbLeTfY0hgw8tZuufeOz0IXVIyp_4toHRzGnIaHqEp5YQEGY-T4_HPGM8m6MzaHSGUJxk5RZMoommc8XCK2lcDFsxBOqVbrCvstoAPSuaqVq7Hsi3xBlrA8Nl50I7Qdt_IFndglC5162SNa7WRDbQOF1DXFuc9Xm-lwp3Rna6V9fPOyMKdo5NK1hYuxjpDbw_L9d1TsHp5fL67XQVFFEdhAEmRyqwiBCqWpkAYZ5KTjOQsjmiUE99jNJfA8jglTMaRLEDGRZgWccjTrIxm6HrI9Qe878E6sdN70_qVImRJmtCMs8RTNwNVGG2tgUp0RjXS9IIS8W1WeLPix6xnr8bEfd5A-Uf-qvTAYgA-VA39_0lieb8eIr8A4FeDjA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2478719647</pqid></control><display><type>article</type><title>Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Bunwanna, Atittaya ; Damrongrungruang, Teerasak ; Puasiri, Subin ; Kantrong, Nutthapong ; Chailertvanitkul, Pattama</creator><creatorcontrib>Bunwanna, Atittaya ; Damrongrungruang, Teerasak ; Puasiri, Subin ; Kantrong, Nutthapong ; Chailertvanitkul, Pattama</creatorcontrib><description>Background/Aim
Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues.
Materials and Methods
PDL cells from human premolars were tested for cytotoxicity of the extract by PrestoBlue assay to determine a non‐toxic concentration. Subsequently, 96 freshly extracted premolars were allocated into different treatment groups. Control groups were freshly extracted premolars or they had been stored dry for 12 hours. Experimental avulsed teeth were created by leaving them air‐dried for 30 minutes immediately after extraction, then they were immersed in Thai propolis extract, HBSS or milk for 3, 6 and 12 hours. After tooth storage, the remaining PDL cells were determined for their cell viability. RNA isolated from PDL tissues of three premolars treated similarly was analysed for periostin and S100A4 expressions using RT‐qPCR.
Results
Thai propolis extract at 0.625 mg mL−1 promoted the greatest PDL cell viability. Tooth storage in 0.625 mg mL−1 Thai propolis extract, HBSS or milk showed no difference in maintaining cell viability. Periostin mRNA level was preserved by Thai propolis extract. Expression of S100A4 mRNA in PDL tissues stored in all tested media was dampened.
Conclusions
PDL cells from mock avulsed teeth stored in 0.625 mg mL−1 Thai propolis extract for 3, 6 and 12 hours remained viable and the expression of periostin was preserved. This study suggests this extract as an alternative for a tooth storage medium for up to 12 hours. However, transporting an avulsed tooth in a storage medium for extended extra‐oral time might affect the PDL cell phenotypes.</description><identifier>ISSN: 1600-4469</identifier><identifier>EISSN: 1600-9657</identifier><identifier>DOI: 10.1111/edt.12612</identifier><identifier>PMID: 33185962</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Animals ; Cell Survival ; Cell viability ; Cytotoxicity ; Gene Expression ; Humans ; Isotonic Solutions ; Ligaments ; Milk ; Organ Preservation Solutions ; Periodontal Ligament ; Phenotypes ; Plant Extracts - pharmacology ; Premolars ; PrestoBlue ; Propolis ; Propolis - pharmacology ; S100A4 ; S100A4 protein ; storage medium ; Thailand ; Tooth Avulsion</subject><ispartof>Dental traumatology, 2021-02, Vol.37 (1), p.123-130</ispartof><rights>2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-e7c8a9f00ef488e0464a6090b45313b0a9f41bae4b5804a53acea5c28c52689d3</citedby><cites>FETCH-LOGICAL-c3532-e7c8a9f00ef488e0464a6090b45313b0a9f41bae4b5804a53acea5c28c52689d3</cites><orcidid>0000-0002-6520-6750</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fedt.12612$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fedt.12612$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33185962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bunwanna, Atittaya</creatorcontrib><creatorcontrib>Damrongrungruang, Teerasak</creatorcontrib><creatorcontrib>Puasiri, Subin</creatorcontrib><creatorcontrib>Kantrong, Nutthapong</creatorcontrib><creatorcontrib>Chailertvanitkul, Pattama</creatorcontrib><title>Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract</title><title>Dental traumatology</title><addtitle>Dent Traumatol</addtitle><description>Background/Aim
Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues.
Materials and Methods
PDL cells from human premolars were tested for cytotoxicity of the extract by PrestoBlue assay to determine a non‐toxic concentration. Subsequently, 96 freshly extracted premolars were allocated into different treatment groups. Control groups were freshly extracted premolars or they had been stored dry for 12 hours. Experimental avulsed teeth were created by leaving them air‐dried for 30 minutes immediately after extraction, then they were immersed in Thai propolis extract, HBSS or milk for 3, 6 and 12 hours. After tooth storage, the remaining PDL cells were determined for their cell viability. RNA isolated from PDL tissues of three premolars treated similarly was analysed for periostin and S100A4 expressions using RT‐qPCR.
Results
Thai propolis extract at 0.625 mg mL−1 promoted the greatest PDL cell viability. Tooth storage in 0.625 mg mL−1 Thai propolis extract, HBSS or milk showed no difference in maintaining cell viability. Periostin mRNA level was preserved by Thai propolis extract. Expression of S100A4 mRNA in PDL tissues stored in all tested media was dampened.
Conclusions
PDL cells from mock avulsed teeth stored in 0.625 mg mL−1 Thai propolis extract for 3, 6 and 12 hours remained viable and the expression of periostin was preserved. This study suggests this extract as an alternative for a tooth storage medium for up to 12 hours. However, transporting an avulsed tooth in a storage medium for extended extra‐oral time might affect the PDL cell phenotypes.</description><subject>Animals</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Cytotoxicity</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Isotonic Solutions</subject><subject>Ligaments</subject><subject>Milk</subject><subject>Organ Preservation Solutions</subject><subject>Periodontal Ligament</subject><subject>Phenotypes</subject><subject>Plant Extracts - pharmacology</subject><subject>Premolars</subject><subject>PrestoBlue</subject><subject>Propolis</subject><subject>Propolis - pharmacology</subject><subject>S100A4</subject><subject>S100A4 protein</subject><subject>storage medium</subject><subject>Thailand</subject><subject>Tooth Avulsion</subject><issn>1600-4469</issn><issn>1600-9657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EoqUw8AeQJSaGtHbiOMmIoHxIlWAos-Ukl9ZVEgfbLeTfY0hgw8tZuufeOz0IXVIyp_4toHRzGnIaHqEp5YQEGY-T4_HPGM8m6MzaHSGUJxk5RZMoommc8XCK2lcDFsxBOqVbrCvstoAPSuaqVq7Hsi3xBlrA8Nl50I7Qdt_IFndglC5162SNa7WRDbQOF1DXFuc9Xm-lwp3Rna6V9fPOyMKdo5NK1hYuxjpDbw_L9d1TsHp5fL67XQVFFEdhAEmRyqwiBCqWpkAYZ5KTjOQsjmiUE99jNJfA8jglTMaRLEDGRZgWccjTrIxm6HrI9Qe878E6sdN70_qVImRJmtCMs8RTNwNVGG2tgUp0RjXS9IIS8W1WeLPix6xnr8bEfd5A-Uf-qvTAYgA-VA39_0lieb8eIr8A4FeDjA</recordid><startdate>202102</startdate><enddate>202102</enddate><creator>Bunwanna, Atittaya</creator><creator>Damrongrungruang, Teerasak</creator><creator>Puasiri, Subin</creator><creator>Kantrong, Nutthapong</creator><creator>Chailertvanitkul, Pattama</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-6520-6750</orcidid></search><sort><creationdate>202102</creationdate><title>Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract</title><author>Bunwanna, Atittaya ; Damrongrungruang, Teerasak ; Puasiri, Subin ; Kantrong, Nutthapong ; Chailertvanitkul, Pattama</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-e7c8a9f00ef488e0464a6090b45313b0a9f41bae4b5804a53acea5c28c52689d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Cell Survival</topic><topic>Cell viability</topic><topic>Cytotoxicity</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Isotonic Solutions</topic><topic>Ligaments</topic><topic>Milk</topic><topic>Organ Preservation Solutions</topic><topic>Periodontal Ligament</topic><topic>Phenotypes</topic><topic>Plant Extracts - pharmacology</topic><topic>Premolars</topic><topic>PrestoBlue</topic><topic>Propolis</topic><topic>Propolis - pharmacology</topic><topic>S100A4</topic><topic>S100A4 protein</topic><topic>storage medium</topic><topic>Thailand</topic><topic>Tooth Avulsion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bunwanna, Atittaya</creatorcontrib><creatorcontrib>Damrongrungruang, Teerasak</creatorcontrib><creatorcontrib>Puasiri, Subin</creatorcontrib><creatorcontrib>Kantrong, Nutthapong</creatorcontrib><creatorcontrib>Chailertvanitkul, Pattama</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Dental traumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bunwanna, Atittaya</au><au>Damrongrungruang, Teerasak</au><au>Puasiri, Subin</au><au>Kantrong, Nutthapong</au><au>Chailertvanitkul, Pattama</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract</atitle><jtitle>Dental traumatology</jtitle><addtitle>Dent Traumatol</addtitle><date>2021-02</date><risdate>2021</risdate><volume>37</volume><issue>1</issue><spage>123</spage><epage>130</epage><pages>123-130</pages><issn>1600-4469</issn><eissn>1600-9657</eissn><abstract>Background/Aim
Success of tooth replantation depends on the quality and quantity of periodontal ligament (PDL) cells. The aims of this study were to evaluate Thai propolis extract as a storage medium for maintaining PDL cell viability and preserving gene expressions in PDL tissues.
Materials and Methods
PDL cells from human premolars were tested for cytotoxicity of the extract by PrestoBlue assay to determine a non‐toxic concentration. Subsequently, 96 freshly extracted premolars were allocated into different treatment groups. Control groups were freshly extracted premolars or they had been stored dry for 12 hours. Experimental avulsed teeth were created by leaving them air‐dried for 30 minutes immediately after extraction, then they were immersed in Thai propolis extract, HBSS or milk for 3, 6 and 12 hours. After tooth storage, the remaining PDL cells were determined for their cell viability. RNA isolated from PDL tissues of three premolars treated similarly was analysed for periostin and S100A4 expressions using RT‐qPCR.
Results
Thai propolis extract at 0.625 mg mL−1 promoted the greatest PDL cell viability. Tooth storage in 0.625 mg mL−1 Thai propolis extract, HBSS or milk showed no difference in maintaining cell viability. Periostin mRNA level was preserved by Thai propolis extract. Expression of S100A4 mRNA in PDL tissues stored in all tested media was dampened.
Conclusions
PDL cells from mock avulsed teeth stored in 0.625 mg mL−1 Thai propolis extract for 3, 6 and 12 hours remained viable and the expression of periostin was preserved. This study suggests this extract as an alternative for a tooth storage medium for up to 12 hours. However, transporting an avulsed tooth in a storage medium for extended extra‐oral time might affect the PDL cell phenotypes.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33185962</pmid><doi>10.1111/edt.12612</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-6520-6750</orcidid></addata></record> |
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| ispartof | Dental traumatology, 2021-02, Vol.37 (1), p.123-130 |
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| language | eng |
| recordid | cdi_proquest_journals_2478719647 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cell Survival Cell viability Cytotoxicity Gene Expression Humans Isotonic Solutions Ligaments Milk Organ Preservation Solutions Periodontal Ligament Phenotypes Plant Extracts - pharmacology Premolars PrestoBlue Propolis Propolis - pharmacology S100A4 S100A4 protein storage medium Thailand Tooth Avulsion |
| title | Preservation of the viability and gene expression of human periodontal ligament cells by Thai propolis extract |
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