Melatonin delivery from PCL scaffold enhances glycosaminoglycans deposition in human chondrocytes – Bioactive scaffold model for cartilage regeneration
[Display omitted] •Melatonin albumin nanoparticle (MNP) embedded polycaprolactone scaffold fabricated.•Controlled release observed for 22 days.•MNP concentration modulated melatonin release from scaffolds.•Significant elevation in Glycosaminoglycans - Human chondrocytes.•Diffusion & dissolution...
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| Veröffentlicht in: | Process biochemistry (1991) 2020-12, Vol.99, p.36-47 |
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| container_title | Process biochemistry (1991) |
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| creator | S., Manjunath Kamath Rao, Subha Krishna D., Jaison K., Sridhar N., Kasthuri V., Gopinath P., Sivaperumal S., Shantanu Patil |
| description | [Display omitted]
•Melatonin albumin nanoparticle (MNP) embedded polycaprolactone scaffold fabricated.•Controlled release observed for 22 days.•MNP concentration modulated melatonin release from scaffolds.•Significant elevation in Glycosaminoglycans - Human chondrocytes.•Diffusion & dissolution mechanism of drug release – mathematical models.
The therapeutic potential of an engineered cartilage construct can be enhanced by sustained delivery of chondrogenic drug like melatonin from 3D porous scaffolds embedded with melatonin loaded bovine serum albumin nanoparticles (MNP). In this study, MNP was synthesized and loaded into polycaprolactone (PCL) scaffolds. 12 % (w/v) and 10 % (w/v) PCL scaffolds were fabricated with different concentrations of MNP. X- ray diffraction and Raman analysis of MNP and scaffolds revealed amorphization of melatonin which is highly desired in drug delivery applications. Additionally, Fourier Transform Infrared spectroscopic analysis confirmed the drug to be chemically inert to fabrication process. Field emission scanning electron microscopic analysis suggested highly interlinked porous scaffold (diameter 50 μm – 300 μm) and MNP diameters in the range of 110−200 nm. Importantly, UV spectrophotometric analysis showed that all groups of scaffolds showed sustained release for 21 days, wherein MNP concentration had an influence on release behaviour of melatonin from scaffolds. Drug release kinetics studied using mathematical models revealed, diffusion and dissolution mechanism of release. Furthermore, in vitro evaluation of MNP loaded scaffolds with Human chondrocytes for 21 days increased glycosaminoglycans deposition significantly. In brief, sustained release of melatonin from polycaprolactone scaffolds increased the therapeutic potential of the engineered construct. |
| doi_str_mv | 10.1016/j.procbio.2020.08.015 |
| format | Article |
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•Melatonin albumin nanoparticle (MNP) embedded polycaprolactone scaffold fabricated.•Controlled release observed for 22 days.•MNP concentration modulated melatonin release from scaffolds.•Significant elevation in Glycosaminoglycans - Human chondrocytes.•Diffusion & dissolution mechanism of drug release – mathematical models.
The therapeutic potential of an engineered cartilage construct can be enhanced by sustained delivery of chondrogenic drug like melatonin from 3D porous scaffolds embedded with melatonin loaded bovine serum albumin nanoparticles (MNP). In this study, MNP was synthesized and loaded into polycaprolactone (PCL) scaffolds. 12 % (w/v) and 10 % (w/v) PCL scaffolds were fabricated with different concentrations of MNP. X- ray diffraction and Raman analysis of MNP and scaffolds revealed amorphization of melatonin which is highly desired in drug delivery applications. Additionally, Fourier Transform Infrared spectroscopic analysis confirmed the drug to be chemically inert to fabrication process. Field emission scanning electron microscopic analysis suggested highly interlinked porous scaffold (diameter 50 μm – 300 μm) and MNP diameters in the range of 110−200 nm. Importantly, UV spectrophotometric analysis showed that all groups of scaffolds showed sustained release for 21 days, wherein MNP concentration had an influence on release behaviour of melatonin from scaffolds. Drug release kinetics studied using mathematical models revealed, diffusion and dissolution mechanism of release. Furthermore, in vitro evaluation of MNP loaded scaffolds with Human chondrocytes for 21 days increased glycosaminoglycans deposition significantly. In brief, sustained release of melatonin from polycaprolactone scaffolds increased the therapeutic potential of the engineered construct.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2020.08.015</identifier><language>eng</language><publisher>Barking: Elsevier Ltd</publisher><subject>Amorphization ; Bovine serum albumin ; Cartilage ; Chondrocyte ; Chondrocytes ; Construction engineering ; Controlled release ; Deposition ; Drug delivery ; Drug release ; Emission analysis ; Fabrication ; Field emission microscopy ; Fourier analysis ; Fourier transforms ; Glycosaminoglycans ; Infrared analysis ; Mathematical models ; Melatonin ; Microscopic analysis ; Nanoparticles ; Polycaprolactone ; Porous scaffolds ; Raman spectroscopy ; Regeneration ; Scaffolds ; Scanning electron microscopy ; Serum albumin ; Spectrophotometry ; Sustained release</subject><ispartof>Process biochemistry (1991), 2020-12, Vol.99, p.36-47</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright Elsevier BV Dec 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-50e7e79ad898efd5beac33b84c0151f4cc00e7a289c6d61274d7a5f8779b7d743</citedby><cites>FETCH-LOGICAL-c337t-50e7e79ad898efd5beac33b84c0151f4cc00e7a289c6d61274d7a5f8779b7d743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.procbio.2020.08.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids></links><search><creatorcontrib>S., Manjunath Kamath</creatorcontrib><creatorcontrib>Rao, Subha Krishna</creatorcontrib><creatorcontrib>D., Jaison</creatorcontrib><creatorcontrib>K., Sridhar</creatorcontrib><creatorcontrib>N., Kasthuri</creatorcontrib><creatorcontrib>V., Gopinath</creatorcontrib><creatorcontrib>P., Sivaperumal</creatorcontrib><creatorcontrib>S., Shantanu Patil</creatorcontrib><title>Melatonin delivery from PCL scaffold enhances glycosaminoglycans deposition in human chondrocytes – Bioactive scaffold model for cartilage regeneration</title><title>Process biochemistry (1991)</title><description>[Display omitted]
•Melatonin albumin nanoparticle (MNP) embedded polycaprolactone scaffold fabricated.•Controlled release observed for 22 days.•MNP concentration modulated melatonin release from scaffolds.•Significant elevation in Glycosaminoglycans - Human chondrocytes.•Diffusion & dissolution mechanism of drug release – mathematical models.
The therapeutic potential of an engineered cartilage construct can be enhanced by sustained delivery of chondrogenic drug like melatonin from 3D porous scaffolds embedded with melatonin loaded bovine serum albumin nanoparticles (MNP). In this study, MNP was synthesized and loaded into polycaprolactone (PCL) scaffolds. 12 % (w/v) and 10 % (w/v) PCL scaffolds were fabricated with different concentrations of MNP. X- ray diffraction and Raman analysis of MNP and scaffolds revealed amorphization of melatonin which is highly desired in drug delivery applications. Additionally, Fourier Transform Infrared spectroscopic analysis confirmed the drug to be chemically inert to fabrication process. Field emission scanning electron microscopic analysis suggested highly interlinked porous scaffold (diameter 50 μm – 300 μm) and MNP diameters in the range of 110−200 nm. Importantly, UV spectrophotometric analysis showed that all groups of scaffolds showed sustained release for 21 days, wherein MNP concentration had an influence on release behaviour of melatonin from scaffolds. Drug release kinetics studied using mathematical models revealed, diffusion and dissolution mechanism of release. Furthermore, in vitro evaluation of MNP loaded scaffolds with Human chondrocytes for 21 days increased glycosaminoglycans deposition significantly. In brief, sustained release of melatonin from polycaprolactone scaffolds increased the therapeutic potential of the engineered construct.</description><subject>Amorphization</subject><subject>Bovine serum albumin</subject><subject>Cartilage</subject><subject>Chondrocyte</subject><subject>Chondrocytes</subject><subject>Construction engineering</subject><subject>Controlled release</subject><subject>Deposition</subject><subject>Drug delivery</subject><subject>Drug release</subject><subject>Emission analysis</subject><subject>Fabrication</subject><subject>Field emission microscopy</subject><subject>Fourier analysis</subject><subject>Fourier transforms</subject><subject>Glycosaminoglycans</subject><subject>Infrared analysis</subject><subject>Mathematical models</subject><subject>Melatonin</subject><subject>Microscopic analysis</subject><subject>Nanoparticles</subject><subject>Polycaprolactone</subject><subject>Porous scaffolds</subject><subject>Raman spectroscopy</subject><subject>Regeneration</subject><subject>Scaffolds</subject><subject>Scanning electron microscopy</subject><subject>Serum albumin</subject><subject>Spectrophotometry</subject><subject>Sustained release</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkctKLDEURQtR8PkJQsBxlUm9khqJNj4u9OU60HFIJyfdaaqSNqkWeuY_OPL3_BJP0w0O7ygHsvc6j51ll4wWjLL2elmsYtAzF4qSlrSgoqCsOchOmOBVXpWdOMS6arq8Yaw6zk5TWlJaMcboSfb1F3o1Bu88MdC7d4gbYmMYyPNkSpJW1obeEPAL5TUkMu83OiQ1OB-2pfIJbauQ3OiCJwhZrAfliV4Eb3CmzYie749PcueC0iPif5lDwIbEhki0iqPr1RxIhDl4iGpLO8-OrOoTXOzfs-z14f5l8pRP_z3-mdxOc11VfMwbChx4p4zoBFjTzEDhx0zUGo_AbK01RYUqRadb07KS14arxgrOuxk3vK7OsqsdF4_4toY0ymVYR48tZVlzUTZtKxiqmp1Kx5BSBCtX0Q0qbiSjcpuCXMp9CnKbgqRC4gDou9n5AFd4dxBl0g7wlsZF0KM0wf2H8ANNG5h7</recordid><startdate>202012</startdate><enddate>202012</enddate><creator>S., Manjunath Kamath</creator><creator>Rao, Subha Krishna</creator><creator>D., Jaison</creator><creator>K., Sridhar</creator><creator>N., Kasthuri</creator><creator>V., Gopinath</creator><creator>P., Sivaperumal</creator><creator>S., Shantanu Patil</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>202012</creationdate><title>Melatonin delivery from PCL scaffold enhances glycosaminoglycans deposition in human chondrocytes – Bioactive scaffold model for cartilage regeneration</title><author>S., Manjunath Kamath ; Rao, Subha Krishna ; D., Jaison ; K., Sridhar ; N., Kasthuri ; V., Gopinath ; P., Sivaperumal ; S., Shantanu Patil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-50e7e79ad898efd5beac33b84c0151f4cc00e7a289c6d61274d7a5f8779b7d743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amorphization</topic><topic>Bovine serum albumin</topic><topic>Cartilage</topic><topic>Chondrocyte</topic><topic>Chondrocytes</topic><topic>Construction engineering</topic><topic>Controlled release</topic><topic>Deposition</topic><topic>Drug delivery</topic><topic>Drug release</topic><topic>Emission analysis</topic><topic>Fabrication</topic><topic>Field emission microscopy</topic><topic>Fourier analysis</topic><topic>Fourier transforms</topic><topic>Glycosaminoglycans</topic><topic>Infrared analysis</topic><topic>Mathematical models</topic><topic>Melatonin</topic><topic>Microscopic analysis</topic><topic>Nanoparticles</topic><topic>Polycaprolactone</topic><topic>Porous scaffolds</topic><topic>Raman spectroscopy</topic><topic>Regeneration</topic><topic>Scaffolds</topic><topic>Scanning electron microscopy</topic><topic>Serum albumin</topic><topic>Spectrophotometry</topic><topic>Sustained release</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>S., Manjunath Kamath</creatorcontrib><creatorcontrib>Rao, Subha Krishna</creatorcontrib><creatorcontrib>D., Jaison</creatorcontrib><creatorcontrib>K., Sridhar</creatorcontrib><creatorcontrib>N., Kasthuri</creatorcontrib><creatorcontrib>V., Gopinath</creatorcontrib><creatorcontrib>P., Sivaperumal</creatorcontrib><creatorcontrib>S., Shantanu Patil</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>S., Manjunath Kamath</au><au>Rao, Subha Krishna</au><au>D., Jaison</au><au>K., Sridhar</au><au>N., Kasthuri</au><au>V., Gopinath</au><au>P., Sivaperumal</au><au>S., Shantanu Patil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin delivery from PCL scaffold enhances glycosaminoglycans deposition in human chondrocytes – Bioactive scaffold model for cartilage regeneration</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2020-12</date><risdate>2020</risdate><volume>99</volume><spage>36</spage><epage>47</epage><pages>36-47</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>[Display omitted]
•Melatonin albumin nanoparticle (MNP) embedded polycaprolactone scaffold fabricated.•Controlled release observed for 22 days.•MNP concentration modulated melatonin release from scaffolds.•Significant elevation in Glycosaminoglycans - Human chondrocytes.•Diffusion & dissolution mechanism of drug release – mathematical models.
The therapeutic potential of an engineered cartilage construct can be enhanced by sustained delivery of chondrogenic drug like melatonin from 3D porous scaffolds embedded with melatonin loaded bovine serum albumin nanoparticles (MNP). In this study, MNP was synthesized and loaded into polycaprolactone (PCL) scaffolds. 12 % (w/v) and 10 % (w/v) PCL scaffolds were fabricated with different concentrations of MNP. X- ray diffraction and Raman analysis of MNP and scaffolds revealed amorphization of melatonin which is highly desired in drug delivery applications. Additionally, Fourier Transform Infrared spectroscopic analysis confirmed the drug to be chemically inert to fabrication process. Field emission scanning electron microscopic analysis suggested highly interlinked porous scaffold (diameter 50 μm – 300 μm) and MNP diameters in the range of 110−200 nm. Importantly, UV spectrophotometric analysis showed that all groups of scaffolds showed sustained release for 21 days, wherein MNP concentration had an influence on release behaviour of melatonin from scaffolds. Drug release kinetics studied using mathematical models revealed, diffusion and dissolution mechanism of release. Furthermore, in vitro evaluation of MNP loaded scaffolds with Human chondrocytes for 21 days increased glycosaminoglycans deposition significantly. In brief, sustained release of melatonin from polycaprolactone scaffolds increased the therapeutic potential of the engineered construct.</abstract><cop>Barking</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2020.08.015</doi><tpages>12</tpages></addata></record> |
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| ispartof | Process biochemistry (1991), 2020-12, Vol.99, p.36-47 |
| issn | 1359-5113 1873-3298 |
| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Amorphization Bovine serum albumin Cartilage Chondrocyte Chondrocytes Construction engineering Controlled release Deposition Drug delivery Drug release Emission analysis Fabrication Field emission microscopy Fourier analysis Fourier transforms Glycosaminoglycans Infrared analysis Mathematical models Melatonin Microscopic analysis Nanoparticles Polycaprolactone Porous scaffolds Raman spectroscopy Regeneration Scaffolds Scanning electron microscopy Serum albumin Spectrophotometry Sustained release |
| title | Melatonin delivery from PCL scaffold enhances glycosaminoglycans deposition in human chondrocytes – Bioactive scaffold model for cartilage regeneration |
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