PAM-less plant genome editing using a CRISPR–SpRY toolbox

The rapid development of the CRISPR–Cas9, –Cas12a and –Cas12b genome editing systems has greatly fuelled basic and translational plant research 1 – 6 . DNA targeting by these Cas nucleases is restricted by their preferred protospacer adjacent motifs (PAMs). The PAM requirement for the most popular S...

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Veröffentlicht in:	Nature plants 2021, Vol.7 (1), p.25-33
Hauptverfasser:	Ren, Qiurong, Sretenovic, Simon, Liu, Shishi, Tang, Xu, Huang, Lan, He, Yao, Liu, Li, Guo, Yachong, Zhong, Zhaohui, Liu, Guanqing, Cheng, Yanhao, Zheng, Xuelian, Pan, Changtian, Yin, Desuo, Zhang, Yingxiao, Li, Wanfeng, Qi, Liwang, Li, Chenghao, Qi, Yiping, Zhang, Yong
Format:	Artikel
Sprache:	eng
Schlagworte:	45/70 631/1647/1511 631/449/447/2311 Adenine B30.2-SPRY Domain - genetics Biomedical and Life Sciences Coniferous trees CRISPR CRISPR-Associated Protein 9 CRISPR-Associated Proteins CRISPR-Cas Systems Cytosine Deoxyribonucleic acid Directed evolution DNA Gene Editing - methods Genetic modification Genome, Plant - genetics Genomes Herbicide resistance Herbicides Homology Larix - genetics Letter Life Sciences Non-homologous end joining Nuclease Oryza - genetics Plant Sciences Protoplasts Rice Site-directed mutagenesis
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