circSETD3 regulates MAPRE1 through miR-615-5p and miR-1538 sponges to promote migration and invasion in nasopharyngeal carcinoma
Circular RNAs (circRNAs) play an essential role in tumorigenesis and development. However, they have rarely been investigated in nasopharyngeal carcinoma (NPC). This study aimed to investigate the role of circRNA in the invasion and metastasis of NPC. We screened and verified the high expression of...
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| Veröffentlicht in: | Oncogene 2021-01, Vol.40 (2), p.307-321 |
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| creator | Tang, Le Xiong, Wei Zhang, Lishen Wang, Dan Wang, Yian Wu, Yingfen Wei, Fang Mo, Yongzhen Hou, Xiangchan Shi, Lei Xiong, Fang Zhang, Shanshan Gong, Zhaojian Liao, Qianjin Xiang, Bo Zhang, Wenling Zhou, Ming Li, Xiaoling Li, Guiyuan Guo, Can Zeng, Zhaoyang |
| description | Circular RNAs (circRNAs) play an essential role in tumorigenesis and development. However, they have rarely been investigated in nasopharyngeal carcinoma (NPC). This study aimed to investigate the role of circRNA in the invasion and metastasis of NPC. We screened and verified the high expression of circSETD3 in NPC cell lines using RNA sequencing (RNA-Seq) and verified the results of NPC biopsy samples using real-time quantitative polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). In vivo and in vitro experiments indicated that circSETD3 could promote NPC cell invasion and migration. We compared the proteomic data of NPC cells before and after the overexpression or knockdown of circSETD3 in combination with bioinformatics prediction and experimental verification. It was found that circSETD3 competitively adsorbs to miR-615-5p and miR-1538 and negates their inhibitory effect on MAPRE1 mRNA, thereby upregulating the expression of MAPRE1. The upregulated MAPRE1 then inhibits the acetylation of α-tubulin, promotes the dynamic assembly of microtubules, and enhances the invasion and migration capabilities of NPC cells. The results of this study suggest that circSETD3 is a novel molecular marker and a potential target for NPC diagnosis and treatment. |
| doi_str_mv | 10.1038/s41388-020-01531-5 |
| format | Article |
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However, they have rarely been investigated in nasopharyngeal carcinoma (NPC). This study aimed to investigate the role of circRNA in the invasion and metastasis of NPC. We screened and verified the high expression of circSETD3 in NPC cell lines using RNA sequencing (RNA-Seq) and verified the results of NPC biopsy samples using real-time quantitative polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). In vivo and in vitro experiments indicated that circSETD3 could promote NPC cell invasion and migration. We compared the proteomic data of NPC cells before and after the overexpression or knockdown of circSETD3 in combination with bioinformatics prediction and experimental verification. It was found that circSETD3 competitively adsorbs to miR-615-5p and miR-1538 and negates their inhibitory effect on MAPRE1 mRNA, thereby upregulating the expression of MAPRE1. The upregulated MAPRE1 then inhibits the acetylation of α-tubulin, promotes the dynamic assembly of microtubules, and enhances the invasion and migration capabilities of NPC cells. The results of this study suggest that circSETD3 is a novel molecular marker and a potential target for NPC diagnosis and treatment.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/s41388-020-01531-5</identifier><identifier>PMID: 33122825</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/105 ; 13/109 ; 13/44 ; 13/89 ; 14/19 ; 38/22 ; 38/47 ; 38/71 ; 38/91 ; 45/90 ; 631/67/1536 ; 631/67/1857 ; 631/67/322 ; 631/67/395 ; 631/80/84 ; 64/60 ; 82/58 ; 82/80 ; Acetylation ; Animals ; Apoptosis ; Bioinformatics ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Biopsy ; Cancer ; Care and treatment ; Cell adhesion & migration ; Cell Biology ; Cell migration ; Cell Movement ; Cell Proliferation ; Cellular proteins ; Development and progression ; Experiments ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Health aspects ; Histone Methyltransferases - genetics ; Hospitals ; Human Genetics ; Humans ; Hybridization ; Internal Medicine ; Laboratories ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - secondary ; Lungs ; Male ; Medical research ; Medicine ; Medicine & Public Health ; Metastases ; Metastasis ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; MicroRNA ; MicroRNAs - genetics ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - metabolism ; Microtubules ; mRNA ; Nasopharyngeal cancer ; Nasopharyngeal carcinoma ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Nasopharyngeal Neoplasms - pathology ; Neoplasm Invasiveness ; Oncology ; Pathogenesis ; Polymerase chain reaction ; Prognosis ; RNA, Circular - genetics ; Survival Rate ; Throat cancer ; Tubulin ; Tumor Cells, Cultured ; Tumorigenesis ; Xenograft Model Antitumor Assays</subject><ispartof>Oncogene, 2021-01, Vol.40 (2), p.307-321</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020</rights><rights>COPYRIGHT 2021 Nature Publishing Group</rights><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-ce02bdb9d818e0a3c76647312462e831af0b5b18ef1019ecc60b14a539b5b79a3</citedby><cites>FETCH-LOGICAL-c508t-ce02bdb9d818e0a3c76647312462e831af0b5b18ef1019ecc60b14a539b5b79a3</cites><orcidid>0000-0003-2641-5434 ; 0000-0003-1635-8173 ; 0000-0002-0648-0565</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33122825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Le</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Zhang, Lishen</creatorcontrib><creatorcontrib>Wang, Dan</creatorcontrib><creatorcontrib>Wang, Yian</creatorcontrib><creatorcontrib>Wu, Yingfen</creatorcontrib><creatorcontrib>Wei, Fang</creatorcontrib><creatorcontrib>Mo, Yongzhen</creatorcontrib><creatorcontrib>Hou, Xiangchan</creatorcontrib><creatorcontrib>Shi, Lei</creatorcontrib><creatorcontrib>Xiong, Fang</creatorcontrib><creatorcontrib>Zhang, Shanshan</creatorcontrib><creatorcontrib>Gong, Zhaojian</creatorcontrib><creatorcontrib>Liao, Qianjin</creatorcontrib><creatorcontrib>Xiang, Bo</creatorcontrib><creatorcontrib>Zhang, Wenling</creatorcontrib><creatorcontrib>Zhou, Ming</creatorcontrib><creatorcontrib>Li, Xiaoling</creatorcontrib><creatorcontrib>Li, Guiyuan</creatorcontrib><creatorcontrib>Guo, Can</creatorcontrib><creatorcontrib>Zeng, Zhaoyang</creatorcontrib><title>circSETD3 regulates MAPRE1 through miR-615-5p and miR-1538 sponges to promote migration and invasion in nasopharyngeal carcinoma</title><title>Oncogene</title><addtitle>Oncogene</addtitle><addtitle>Oncogene</addtitle><description>Circular RNAs (circRNAs) play an essential role in tumorigenesis and development. However, they have rarely been investigated in nasopharyngeal carcinoma (NPC). This study aimed to investigate the role of circRNA in the invasion and metastasis of NPC. We screened and verified the high expression of circSETD3 in NPC cell lines using RNA sequencing (RNA-Seq) and verified the results of NPC biopsy samples using real-time quantitative polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). In vivo and in vitro experiments indicated that circSETD3 could promote NPC cell invasion and migration. We compared the proteomic data of NPC cells before and after the overexpression or knockdown of circSETD3 in combination with bioinformatics prediction and experimental verification. It was found that circSETD3 competitively adsorbs to miR-615-5p and miR-1538 and negates their inhibitory effect on MAPRE1 mRNA, thereby upregulating the expression of MAPRE1. The upregulated MAPRE1 then inhibits the acetylation of α-tubulin, promotes the dynamic assembly of microtubules, and enhances the invasion and migration capabilities of NPC cells. The results of this study suggest that circSETD3 is a novel molecular marker and a potential target for NPC diagnosis and treatment.</description><subject>13/1</subject><subject>13/105</subject><subject>13/109</subject><subject>13/44</subject><subject>13/89</subject><subject>14/19</subject><subject>38/22</subject><subject>38/47</subject><subject>38/71</subject><subject>38/91</subject><subject>45/90</subject><subject>631/67/1536</subject><subject>631/67/1857</subject><subject>631/67/322</subject><subject>631/67/395</subject><subject>631/80/84</subject><subject>64/60</subject><subject>82/58</subject><subject>82/80</subject><subject>Acetylation</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biopsy</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Cell adhesion & migration</subject><subject>Cell 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Fang</au><au>Mo, Yongzhen</au><au>Hou, Xiangchan</au><au>Shi, Lei</au><au>Xiong, Fang</au><au>Zhang, Shanshan</au><au>Gong, Zhaojian</au><au>Liao, Qianjin</au><au>Xiang, Bo</au><au>Zhang, Wenling</au><au>Zhou, Ming</au><au>Li, Xiaoling</au><au>Li, Guiyuan</au><au>Guo, Can</au><au>Zeng, Zhaoyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>circSETD3 regulates MAPRE1 through miR-615-5p and miR-1538 sponges to promote migration and invasion in nasopharyngeal carcinoma</atitle><jtitle>Oncogene</jtitle><stitle>Oncogene</stitle><addtitle>Oncogene</addtitle><date>2021-01-14</date><risdate>2021</risdate><volume>40</volume><issue>2</issue><spage>307</spage><epage>321</epage><pages>307-321</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>Circular RNAs (circRNAs) play an essential role in tumorigenesis and development. However, they have rarely been investigated in nasopharyngeal carcinoma (NPC). This study aimed to investigate the role of circRNA in the invasion and metastasis of NPC. We screened and verified the high expression of circSETD3 in NPC cell lines using RNA sequencing (RNA-Seq) and verified the results of NPC biopsy samples using real-time quantitative polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). In vivo and in vitro experiments indicated that circSETD3 could promote NPC cell invasion and migration. We compared the proteomic data of NPC cells before and after the overexpression or knockdown of circSETD3 in combination with bioinformatics prediction and experimental verification. It was found that circSETD3 competitively adsorbs to miR-615-5p and miR-1538 and negates their inhibitory effect on MAPRE1 mRNA, thereby upregulating the expression of MAPRE1. The upregulated MAPRE1 then inhibits the acetylation of α-tubulin, promotes the dynamic assembly of microtubules, and enhances the invasion and migration capabilities of NPC cells. The results of this study suggest that circSETD3 is a novel molecular marker and a potential target for NPC diagnosis and treatment.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33122825</pmid><doi>10.1038/s41388-020-01531-5</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-2641-5434</orcidid><orcidid>https://orcid.org/0000-0003-1635-8173</orcidid><orcidid>https://orcid.org/0000-0002-0648-0565</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-9232 |
| ispartof | Oncogene, 2021-01, Vol.40 (2), p.307-321 |
| issn | 0950-9232 1476-5594 |
| language | eng |
| recordid | cdi_proquest_journals_2477821437 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 13/1 13/105 13/109 13/44 13/89 14/19 38/22 38/47 38/71 38/91 45/90 631/67/1536 631/67/1857 631/67/322 631/67/395 631/80/84 64/60 82/58 82/80 Acetylation Animals Apoptosis Bioinformatics Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biopsy Cancer Care and treatment Cell adhesion & migration Cell Biology Cell migration Cell Movement Cell Proliferation Cellular proteins Development and progression Experiments Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Health aspects Histone Methyltransferases - genetics Hospitals Human Genetics Humans Hybridization Internal Medicine Laboratories Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - secondary Lungs Male Medical research Medicine Medicine & Public Health Metastases Metastasis Mice Mice, Inbred BALB C Mice, Nude MicroRNA MicroRNAs - genetics Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Microtubules mRNA Nasopharyngeal cancer Nasopharyngeal carcinoma Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Nasopharyngeal Neoplasms - pathology Neoplasm Invasiveness Oncology Pathogenesis Polymerase chain reaction Prognosis RNA, Circular - genetics Survival Rate Throat cancer Tubulin Tumor Cells, Cultured Tumorigenesis Xenograft Model Antitumor Assays |
| title | circSETD3 regulates MAPRE1 through miR-615-5p and miR-1538 sponges to promote migration and invasion in nasopharyngeal carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T14%3A59%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=circSETD3%20regulates%20MAPRE1%20through%20miR-615-5p%20and%20miR-1538%20sponges%20to%20promote%20migration%20and%20invasion%20in%20nasopharyngeal%20carcinoma&rft.jtitle=Oncogene&rft.au=Tang,%20Le&rft.date=2021-01-14&rft.volume=40&rft.issue=2&rft.spage=307&rft.epage=321&rft.pages=307-321&rft.issn=0950-9232&rft.eissn=1476-5594&rft_id=info:doi/10.1038/s41388-020-01531-5&rft_dat=%3Cgale_proqu%3EA648482689%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2477821437&rft_id=info:pmid/33122825&rft_galeid=A648482689&rfr_iscdi=true |